FastQCFastQC Report
Tue 24 May 2016
ERR840998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379205
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG178080.40664915207212265No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC153400.35029189087973733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA140200.32014943351590075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA139690.3189848385722979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA120810.27587199046402255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA114320.26105194892680295No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA101320.23136619546241843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA94980.2168886818497878No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA77060.17596801245888238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73730.16836389253300543No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG72570.1657150099161834No Hit
AAAAAAAAAAAAAAAAAAAAAAAA72050.16452757977760804No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA70710.16146766365127918No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT59330.13548121177245642No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT55980.12783142145663426No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.12742038794712737No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT54040.12340139363194917No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT52500.11988477360616824No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50870.11616263682563388No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC46950.10721124039637332No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG46780.10682304208183907No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT44370.10131976009344161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT207.975657E-490.643443
AACGACA4950.087.8966443
TCCGGGC4700.077.14332643
TGGTACG702.7560236E-670.3564544
TCGTGCG3200.069.8709643
CCTTAGC30950.064.8219943
TTAGAGT24750.060.49233244
ACGACTA403.3293873E-759.03737342
TCGCAAA4550.057.1086743
CTATCAT19150.055.03600744
GACCGAC601.8189894E-1254.96025541
ACCTTAG24300.054.5832542
TATCGCA655.820766E-1154.49603742
ATTAGTC2900.054.3442944
GCCGCAA1451.2823875E-954.3442944
TCGATCA550.006344613653.7267444
AACCTTA35300.052.50028241
GGTAGAC1709.094947E-1152.1465544
ATCGCAA855.4906195E-849.7650143
ATCGTCG200.00879892849.46423341
Produced by FastQC (version 0.11.5)