##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840998.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4379205
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.145681008310866	39.0	37.0	39.0	34.0	39.0
2	37.82428545820532	39.0	37.0	39.0	35.0	39.0
3	38.05374788346287	39.0	38.0	39.0	35.0	39.0
4	38.411165268581854	39.0	38.0	40.0	35.0	41.0
5	39.16133818809578	40.0	38.0	41.0	35.0	41.0
6	39.1790352358476	40.0	38.0	41.0	35.0	41.0
7	39.18434556043848	40.0	38.0	41.0	35.0	41.0
8	39.12515056956685	40.0	38.0	41.0	35.0	41.0
9	39.15689902619311	40.0	38.0	41.0	35.0	41.0
10	39.13129483547813	40.0	39.0	41.0	35.0	41.0
11	39.05314663277924	40.0	38.0	41.0	35.0	41.0
12	38.984882187520334	40.0	38.0	41.0	35.0	41.0
13	38.90539766921165	40.0	38.0	41.0	35.0	41.0
14	38.831694793918075	40.0	38.0	41.0	35.0	41.0
15	38.72648049132206	40.0	38.0	41.0	35.0	41.0
16	38.63772374209474	40.0	38.0	41.0	35.0	41.0
17	38.60498515141447	40.0	38.0	41.0	35.0	41.0
18	38.585059388633326	40.0	38.0	41.0	35.0	41.0
19	38.56210864757416	40.0	38.0	41.0	35.0	41.0
20	38.475868565184776	40.0	37.0	41.0	35.0	41.0
21	38.387426256592235	40.0	37.0	41.0	35.0	41.0
22	38.28742888263966	40.0	37.0	41.0	34.0	41.0
23	38.1886650659195	40.0	37.0	41.0	34.0	41.0
24	38.03915596552342	40.0	36.0	41.0	34.0	41.0
25	37.94074071179372	39.0	36.0	41.0	33.0	41.0
26	37.798404347012124	39.0	36.0	41.0	33.0	41.0
27	37.711657751010684	39.0	36.0	41.0	33.0	41.0
28	37.612113466913044	39.0	36.0	41.0	33.0	41.0
29	37.546012313901294	39.0	36.0	41.0	33.0	41.0
30	37.531602508658345	39.0	36.0	41.0	33.0	41.0
31	37.45409302673869	39.0	35.0	40.0	33.0	41.0
32	37.414970260327664	39.0	35.0	40.0	33.0	41.0
33	37.28638536021765	38.0	35.0	40.0	32.0	41.0
34	37.24363337683535	38.0	35.0	40.0	32.0	41.0
35	37.14584906065703	38.0	35.0	40.0	32.0	41.0
36	37.11954105581996	38.0	35.0	40.0	32.0	41.0
37	37.03725348932235	38.0	35.0	40.0	32.0	41.0
38	36.883390000350516	38.0	35.0	40.0	32.0	41.0
39	36.74987155656841	38.0	35.0	40.0	31.0	41.0
40	36.53405783932127	38.0	34.0	40.0	31.0	41.0
41	36.43711051707744	37.0	34.0	40.0	31.0	41.0
42	36.25205132099885	37.0	34.0	40.0	31.0	41.0
43	36.1047983106245	37.0	34.0	40.0	30.0	41.0
44	35.90328714985824	37.0	34.0	40.0	30.0	41.0
45	35.67186879335988	37.0	34.0	40.0	29.0	41.0
46	35.36290599380772	36.0	33.0	40.0	28.0	41.0
47	35.392180798669266	36.0	33.0	39.0	29.0	41.0
48	36.2091579589303	37.0	34.0	40.0	31.0	41.0
49	37.029436550834205	38.0	35.0	40.0	32.0	41.0
50	36.800829115228694	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	17.0
21	58.0
22	133.0
23	223.0
24	455.0
25	839.0
26	1613.0
27	3317.0
28	6845.0
29	14185.0
30	27853.0
31	52961.0
32	95617.0
33	163299.0
34	243435.0
35	278912.0
36	487375.0
37	766060.0
38	719725.0
39	687130.0
40	829148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.99884111385514	14.825156620893518	30.176002265251338
2	25.97884775889688	26.270361857917134	28.677648111928995	19.073142271256998
3	24.95573968334435	24.177767425822726	26.38901353099478	24.477479359838146
4	21.21222916031563	24.346862044594854	30.79935741761347	23.64155137747605
5	19.1134007199937	28.42251047850009	29.07388441509361	23.390204386412602
6	21.952386334962625	30.59642103989194	27.05870129395632	20.39249133118911
7	26.098161652628733	29.37713580432978	23.89863000247762	20.626072540563868
8	23.942450741630044	26.644722044297996	26.852979022448135	22.55984819162382
9	23.687724141710653	28.027735627813726	27.194639209628228	21.08990102084739
10	25.288471309290156	25.27314889346354	27.05710283030824	22.381276966938064
11	24.904360494655993	26.329642024066015	26.487570232496537	22.27842724878146
12	23.846383076380302	26.034725480994837	27.14077098468786	22.978120457937003
13	23.268355786038793	26.57418412702762	26.104806694365756	24.05265339256783
14	22.550051892980573	26.622457729199706	27.170707925296945	23.656782452522776
15	20.19919140574602	29.67273740325013	27.25104214121056	22.877029049793286
16	22.87157143819483	32.78170352838015	23.725607730170204	20.621117303254817
17	24.303292492587126	32.577465544545184	21.459260299529255	21.65998166333844
18	24.04144131183628	27.84395797867421	24.221930692899736	23.892670016589772
19	24.62373878363767	26.540205356908388	25.4147956078786	23.421260251575344
20	25.326857272039106	26.976448921665003	24.757530190982152	22.93916361531374
21	24.51700708233572	28.208179338487238	24.725880610750124	22.548932968426918
22	25.55146424978963	29.175706549476445	22.81656145350583	22.456267747228093
23	25.60451040771099	29.118687067629857	24.08448565435964	21.192316870299518
24	25.371066209506065	29.35509984118122	24.26536323373763	21.008470715575086
25	24.041215377333703	29.29209803364013	25.424961168639477	21.24172542038669
26	24.601828184401395	30.270603914386918	25.866293047041967	19.261274854169717
27	24.59157257211513	29.022137396550534	24.667681925547942	21.71860810578639
28	26.029425905882746	28.870871292317197	23.532360912352875	21.567341889447185
29	25.88866849731804	28.712037392678543	25.498389103229247	19.900905006774174
30	27.620260830018978	27.456346377246916	25.611186908074966	19.312205884659136
31	24.254595322381007	28.534116998716243	26.213954549169717	20.997333129733033
32	24.257717823906184	27.61076898542467	26.806635104317202	21.32487808635194
33	22.873289508403488	27.433047299379854	28.128194771855135	21.565468420361526
34	23.149796965495923	27.787779395096162	26.711694014734487	22.35072962467342
35	23.267854592968227	27.42133389608404	26.61211017614181	22.69870133480592
36	24.23110666900081	27.464158344495154	25.83433755786165	22.470397428642382
37	23.506009857866886	29.649920786826954	24.914642301575356	21.9294270537308
38	23.609218201349698	28.201024954793535	25.041907475716346	23.14784936814042
39	23.91026673440907	28.540662169006918	25.527655472312627	22.021415624271388
40	21.74077555785959	28.283399338164827	26.800376024883537	23.17544907909205
41	23.187367216111177	29.80929233358082	25.326336806540056	21.677003643767947
42	22.631553885798514	30.79092340502394	24.37811289374789	22.199409815429657
43	22.8925444200842	28.633139198622676	25.02554238906748	23.448773992225643
44	23.040476434794492	28.7093612764484	24.87953866642815	23.370623622328957
45	21.49376795595422	28.16613703337614	27.401074816860817	22.939020193808833
46	22.475200595320313	29.320070236003353	25.738405819627815	22.466323349048515
47	23.940454334653076	28.70905181500063	25.191656861204482	22.158836989141808
48	22.94589945606843	29.61546490333296	25.349654015039018	22.088981625559587
49	21.884343426192505	28.92951043867485	24.46396231088607	24.722183824246578
50	21.301161219394885	30.816976248826183	26.190650695128376	21.69121183665056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127397.0
1	126606.5
2	103039.0
3	78383.5
4	72375.0
5	49483.0
6	22718.0
7	15379.5
8	15986.0
9	15183.0
10	13607.0
11	11597.5
12	9677.0
13	8553.5
14	8702.0
15	9510.0
16	10437.5
17	11835.5
18	12796.5
19	13401.0
20	14443.0
21	16815.5
22	20050.0
23	25715.5
24	32424.5
25	38358.0
26	44771.0
27	52238.0
28	59531.0
29	65070.5
30	70537.0
31	81465.5
32	99166.0
33	113556.0
34	133016.5
35	157085.5
36	172830.5
37	184851.0
38	194947.0
39	207505.5
40	224806.5
41	245183.0
42	267702.0
43	290339.0
44	309610.5
45	318059.0
46	321239.5
47	323255.0
48	341313.0
49	370644.5
50	401673.5
51	408918.5
52	379198.0
53	352786.5
54	330459.0
55	311006.0
56	292080.5
57	271184.0
58	244889.5
59	213465.5
60	185501.5
61	156980.0
62	130829.0
63	105733.5
64	86768.5
65	76013.5
66	60558.0
67	43650.0
68	32771.0
69	25984.0
70	20611.0
71	15569.5
72	11894.0
73	9114.0
74	6102.0
75	4034.5
76	3135.0
77	2150.0
78	1507.5
79	1258.0
80	931.0
81	754.5
82	430.0
83	149.5
84	136.0
85	136.0
86	120.0
87	88.0
88	50.5
89	37.5
90	29.0
91	14.5
92	5.5
93	5.0
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	28984.0
25	34245.0
26	37921.0
27	45526.0
28	46041.0
29	41643.0
30	58407.0
31	35620.0
32	37425.0
33	27371.0
34	29267.0
35	26691.0
36	24148.0
37	25940.0
38	24193.0
39	31568.0
40	32810.0
41	32242.0
42	41813.0
43	72612.0
44	180706.0
45	343695.0
46	511247.0
47	415064.0
48	768893.0
49	551913.0
50	873220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.909517796065126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39657498199365	9.346048731042103
2	12.079710456631004	3.1188647422238382
3	4.047261340441756	1.5674457689927774
4	1.8666163595997882	0.9638846845071906
5	1.0171536161602233	0.6565481354576198
6	0.7101708807144326	0.5500778173698125
7	0.5325039221314147	0.48120582014609836
8	0.3905253987065348	0.40331956675339514
9	0.30865682065972333	0.3586149647265222
>10	3.1027306563377315	9.380431988343844
>50	1.6445069075008016	15.838136272154891
>100	1.8141983151269816	37.8534228824678
>500	0.05051263882947814	4.501791895234899
>1k	0.03348590663976641	8.61518499426134
>5k	0.0034053464379423465	2.892732102076252
>10k+	0.0019864520887997025	3.4722896342416774
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	17808	0.40664915207212265	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	15340	0.35029189087973733	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	14020	0.32014943351590075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	13969	0.3189848385722979	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	12081	0.27587199046402255	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11432	0.26105194892680295	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10132	0.23136619546241843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9498	0.2168886818497878	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	7706	0.17596801245888238	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7373	0.16836389253300543	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	7257	0.1657150099161834	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	7205	0.16452757977760804	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7071	0.16146766365127918	No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT	5933	0.13548121177245642	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5598	0.12783142145663426	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5580	0.12742038794712737	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5404	0.12340139363194917	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	5250	0.11988477360616824	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5087	0.11616263682563388	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	4695	0.10721124039637332	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	4678	0.10682304208183907	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	4437	0.10131976009344161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10558994155331847	0.0	0.0	0.0	0.0
2	0.21638630756039054	0.0	0.0	0.0	0.0
3	0.2715561386142005	0.0	0.0	0.0	0.0
4	0.31489733867220193	0.0	0.0	0.0	0.0
5	0.3584440554849567	0.0	0.0	0.0	0.0
6	0.4108508279470817	0.0	0.0	0.0	0.0
7	0.4515431453882611	0.0	0.0	0.0	0.0
8	0.4931488706283446	0.0	0.0	0.0	0.0
9	0.5346175846985926	0.0	0.0	0.0	0.0
10	0.5746248462905938	0.0	0.0	0.0	0.0
11	0.6165045938703486	0.0	0.0	0.0	0.0
12	0.6558496348081444	0.0	0.0	0.0	0.0
13	0.6982774270672416	0.0	0.0	0.0	0.0
14	0.736206685916736	0.0	0.0	0.0	0.0
15	0.7760769363389017	0.0	0.0	0.0	0.0
16	0.8096446729486289	0.0	0.0	0.0	0.0
17	0.8509535406540685	0.0	0.0	0.0	2.2835194972603476E-5
18	0.8784014450111378	0.0	0.0	0.0	2.2835194972603476E-5
19	0.913750326828728	0.0	0.0	0.0	2.2835194972603476E-5
20	0.943299069123277	0.0	0.0	0.0	2.2835194972603476E-5
21	0.9720714147887574	0.0	0.0	0.0	2.2835194972603476E-5
22	0.9998161766804705	0.0	0.0	0.0	2.2835194972603476E-5
23	1.027720784936992	0.0	0.0	0.0	2.2835194972603476E-5
24	1.0566529769672806	0.0	0.0	0.0	2.2835194972603476E-5
25	1.0840095405444596	0.0	0.0	0.0	2.2835194972603476E-5
26	1.1257066065644334	0.0	0.0	0.0	2.2835194972603476E-5
27	1.1559860750981057	0.0	0.0	0.0	2.2835194972603476E-5
28	1.1878411720848876	0.0	0.0	0.0	2.2835194972603476E-5
29	1.222185305323683	0.0	0.0	0.0	2.2835194972603476E-5
30	1.2538577207506842	0.0	0.0	0.0	2.2835194972603476E-5
31	1.2820363513468769	0.0	0.0	0.0	2.2835194972603476E-5
32	1.3132748980693985	0.0	0.0	0.0	2.2835194972603476E-5
33	1.3430291571187007	0.0	0.0	0.0	2.2835194972603476E-5
34	1.3736054831870168	0.0	0.0	0.0	2.2835194972603476E-5
35	1.3998659574055108	0.0	0.0	0.0	2.2835194972603476E-5
36	1.4234547138122102	0.0	0.0	0.0	2.2835194972603476E-5
37	1.4438465429227452	0.0	0.0	0.0	2.2835194972603476E-5
38	1.4571822967867456	0.0	0.0	0.0	2.2835194972603476E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGT	20	7.975657E-4	90.6434	43
AACGACA	495	0.0	87.89664	43
TCCGGGC	470	0.0	77.143326	43
TGGTACG	70	2.7560236E-6	70.35645	44
TCGTGCG	320	0.0	69.87096	43
CCTTAGC	3095	0.0	64.82199	43
TTAGAGT	2475	0.0	60.492332	44
ACGACTA	40	3.3293873E-7	59.037373	42
TCGCAAA	455	0.0	57.10867	43
CTATCAT	1915	0.0	55.036007	44
GACCGAC	60	1.8189894E-12	54.960255	41
ACCTTAG	2430	0.0	54.58325	42
TATCGCA	65	5.820766E-11	54.496037	42
ATTAGTC	290	0.0	54.34429	44
GCCGCAA	145	1.2823875E-9	54.34429	44
TCGATCA	55	0.0063446136	53.72674	44
AACCTTA	3530	0.0	52.500282	41
GGTAGAC	170	9.094947E-11	52.14655	44
ATCGCAA	85	5.4906195E-8	49.76501	43
ATCGTCG	20	0.008798928	49.464233	41
>>END_MODULE