##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840997.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884826 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88890910885143 39.0 37.0 39.0 32.0 39.0 2 37.62364801843778 39.0 37.0 39.0 35.0 39.0 3 37.88538570669123 39.0 38.0 39.0 35.0 39.0 4 38.35931009016217 39.0 38.0 40.0 35.0 41.0 5 38.94053880835685 40.0 38.0 41.0 35.0 41.0 6 38.94333482241862 40.0 38.0 41.0 35.0 41.0 7 38.93591875324301 40.0 38.0 41.0 35.0 41.0 8 38.906376503719706 40.0 38.0 41.0 35.0 41.0 9 38.900431127329526 40.0 38.0 41.0 35.0 41.0 10 38.9163599186344 40.0 38.0 41.0 35.0 41.0 11 38.75330614072599 40.0 38.0 41.0 35.0 41.0 12 38.69288358713218 40.0 38.0 41.0 35.0 41.0 13 38.59538811540163 40.0 38.0 41.0 35.0 41.0 14 38.485815136251304 40.0 38.0 41.0 35.0 41.0 15 38.4113530904179 40.0 38.0 41.0 34.0 41.0 16 38.383684753924236 40.0 38.0 41.0 35.0 41.0 17 38.39473351916835 40.0 38.0 41.0 35.0 41.0 18 38.390644547560356 40.0 38.0 41.0 35.0 41.0 19 38.41195420691353 40.0 37.0 41.0 34.0 41.0 20 38.28055427928095 40.0 37.0 41.0 34.0 41.0 21 38.163341337608884 40.0 37.0 41.0 34.0 41.0 22 38.09751669384866 40.0 37.0 41.0 34.0 41.0 23 38.00674916411382 39.0 36.0 41.0 34.0 41.0 24 37.79446272494119 39.0 36.0 41.0 33.0 41.0 25 37.70032066859723 39.0 36.0 41.0 33.0 41.0 26 37.544490598979245 39.0 36.0 40.0 33.0 41.0 27 37.38324377203465 39.0 35.0 40.0 33.0 41.0 28 37.263235741203694 38.0 35.0 40.0 33.0 41.0 29 37.20068814548101 38.0 35.0 40.0 32.0 41.0 30 37.17096438929307 38.0 35.0 40.0 33.0 41.0 31 37.108806854045746 38.0 35.0 40.0 32.0 41.0 32 37.01425104086823 38.0 35.0 40.0 32.0 41.0 33 36.85015687054235 38.0 35.0 40.0 32.0 41.0 34 36.75723498099557 38.0 35.0 40.0 32.0 41.0 35 36.58440765168841 38.0 35.0 40.0 31.0 41.0 36 36.57156087908621 37.0 35.0 40.0 31.0 41.0 37 36.41455666281066 37.0 34.0 40.0 31.0 41.0 38 36.18842688194987 37.0 34.0 40.0 31.0 41.0 39 36.17826884764291 37.0 34.0 40.0 31.0 41.0 40 35.93951257894439 36.0 34.0 40.0 31.0 41.0 41 35.82163855801504 36.0 34.0 40.0 30.0 41.0 42 35.68688106567024 36.0 34.0 39.0 30.0 41.0 43 35.524494608336816 36.0 34.0 39.0 30.0 41.0 44 35.19904052811742 36.0 33.0 39.0 29.0 41.0 45 34.99008699587984 35.0 33.0 39.0 29.0 41.0 46 34.70955304152496 35.0 32.0 39.0 28.0 41.0 47 34.84430967741935 35.0 33.0 39.0 29.0 40.0 48 35.52163096317092 36.0 34.0 39.0 31.0 40.0 49 36.220829222395736 37.0 35.0 39.0 32.0 41.0 50 36.28819378819379 37.0 35.0 39.0 32.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 6.0 21 27.0 22 62.0 23 131.0 24 265.0 25 458.0 26 848.0 27 1689.0 28 3659.0 29 7620.0 30 14858.0 31 27697.0 32 49945.0 33 83762.0 34 124853.0 35 150918.0 36 242584.0 37 334359.0 38 300107.0 39 286770.0 40 254208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.459128853273455 18.23892497238472 34.30194617434182 2 23.987360106450144 22.754195878028 32.29629684649936 20.9621471690225 3 23.65130786608419 24.195867416939283 22.73101071398633 29.4218140029902 4 20.0268884236529 27.055547833062576 27.44741424407346 25.470149499211068 5 14.949496664413587 30.137636047040946 27.34735195715679 27.565515331388678 6 23.372714510517152 26.26571365208247 25.72385992128716 24.63771191611321 7 21.894434817855863 26.64590789812959 23.262624772790698 28.197032511223846 8 19.867404206011592 25.82227749404985 26.616462209243718 27.693856090694847 9 20.974296831643876 30.061448643004713 25.692132854703832 23.272121670647582 10 29.279360535136927 21.908070028745357 26.80915904173648 22.00341039438123 11 23.222727190732726 23.776942805330574 25.256177493307074 27.744152510629625 12 21.20991539802613 27.49909010168578 25.526812554580637 25.764181945707453 13 24.164883124490004 26.237276013807108 23.545462551980926 26.05237830972196 14 19.059849556404675 28.04062550070935 22.866513938156626 30.03301100472935 15 20.87704647537757 33.5148708687168 24.626835580578792 20.98124707532685 16 20.5269345817598 34.10595991354109 21.15728454509859 24.209820959600513 17 20.65050036448988 36.613830666597345 19.62945120663658 23.1062177622762 18 19.731370428888397 30.048609261544566 23.670938325341435 26.549081984225598 19 23.411550986669326 24.477219647861396 25.063586771404893 27.047642594064385 20 23.364968437404833 24.532874652620453 26.774620044502782 25.327536865471934 21 21.511269475272517 32.75188266715336 22.559695165495384 23.17715269207874 22 24.736394765352347 27.880186287752824 21.87109048792833 25.512328458966504 23 26.742627701443 25.518854260287156 26.105858047374138 21.63265999089571 24 20.770458387140245 28.675909606510096 24.459658345120452 26.093973661229207 25 21.10847507234271 25.499883033939387 26.749069879476888 26.64257201424102 26 23.059219142723293 32.13703862871011 24.650483833751164 20.153258394815442 27 23.239490435503672 28.36510659284973 23.363602613401405 25.031800358245192 28 23.16817910408588 25.897128009291155 23.969617833419726 26.96507505320324 29 23.475465518287137 30.102339584355718 24.5343162932779 21.887878604079248 30 24.929272415492132 30.424109982296578 25.147357235266636 19.49926036694465 31 24.49810778738768 29.31226698011844 22.801091844549614 23.388533387944264 32 21.32194078503296 29.380811196584723 24.482022958692315 24.81522505969 33 20.907108824915156 24.587513366897042 30.384060790682227 24.12131701750558 34 21.751135910355927 29.821257456202687 25.811528286574937 22.61607834686645 35 19.4459937649064 25.856662061964485 25.99618742195318 28.701156751175926 36 22.002382366313757 26.04660576592961 25.5679867496822 26.38302511807444 37 18.703519312503136 32.66077021220466 22.09868876455777 26.537021710734432 38 22.47141872124458 26.888651017722186 23.17550953708586 27.464420723947374 39 20.26277211691928 30.727768063217642 27.288123866456697 21.72133595340638 40 17.86987997770631 27.15394863510779 29.271133550508015 25.705037836677885 41 18.9030556840681 30.74502716611488 24.080289691676178 26.271627458140838 42 19.078974123651204 33.28720252456921 23.558882638973614 24.074940712805972 43 19.53532214880499 24.662253445014862 26.12752081746948 29.674903588710666 44 19.25708387064386 27.54763630430758 22.843274754719705 30.352005070328854 45 17.799609949612947 25.526268495776673 30.953094111304143 25.721027443306244 46 19.017520040939786 30.301436414082268 26.44714728957481 24.23389625540313 47 21.506206896551724 29.648142380422694 26.391012235817573 22.45463848720801 48 22.42116273311978 28.421116924449507 23.084079632919856 26.073640709510858 49 18.274124942011277 28.816474677713384 22.641410808444466 30.267989571830878 50 17.875952875952876 28.037863037863037 27.992187992187993 26.09399609399609 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45717.0 1 42560.0 2 30140.5 3 19954.5 4 17485.0 5 11945.5 6 5776.0 7 3636.5 8 3433.5 9 2991.0 10 2689.5 11 2594.5 12 2465.0 13 2292.0 14 2429.5 15 2607.0 16 2521.0 17 2465.0 18 2738.0 19 3292.0 20 3665.0 21 3654.5 22 3643.5 23 4790.5 24 6799.5 25 8361.0 26 10251.5 27 12407.0 28 13445.0 29 14187.5 30 18114.0 31 30528.5 32 48757.5 33 55519.5 34 63268.5 35 79549.0 36 80091.5 37 76058.5 38 77418.0 39 77950.0 40 82529.0 41 93356.0 42 107582.0 43 121408.0 44 131270.5 45 138957.5 46 147457.0 47 149188.5 48 158351.5 49 184917.5 50 219901.0 51 239599.0 52 221996.0 53 182153.5 54 146362.5 55 130138.0 56 122834.5 57 112929.5 58 100073.0 59 85876.5 60 75581.5 61 64931.0 62 52787.5 63 43085.5 64 35365.0 65 29281.0 66 22009.0 67 14799.5 68 10045.0 69 7245.0 70 5557.0 71 4448.0 72 3499.5 73 2774.0 74 2056.5 75 1447.5 76 1062.0 77 706.5 78 471.0 79 346.0 80 269.0 81 162.0 82 67.0 83 42.0 84 36.0 85 31.5 86 24.0 87 16.5 88 9.5 89 4.5 90 3.0 91 3.0 92 2.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 12488.0 25 13125.0 26 14108.0 27 16269.0 28 15266.0 29 13353.0 30 17912.0 31 11908.0 32 15128.0 33 10413.0 34 11121.0 35 9399.0 36 10990.0 37 12145.0 38 10154.0 39 13442.0 40 15424.0 41 14692.0 42 19049.0 43 34855.0 44 91215.0 45 167727.0 46 210893.0 47 206893.0 48 304669.0 49 294728.0 50 317460.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.5023112189003 #Duplication Level Percentage of deduplicated Percentage of total 1 68.70908520945152 7.9031328164505075 2 11.606060167008685 2.6699303213243177 3 4.4078103436848926 1.5210001910085453 4 2.3105666384755255 1.0630742627101515 5 1.4076345247243196 0.8095525192923966 6 0.9680595606219776 0.6680953406823519 7 0.7258683713518046 0.5844414737871326 8 0.5554870182923103 0.5111507649965693 9 0.4790407328418159 0.4959068036108975 >10 4.8470770675832755 13.16039645600913 >50 2.238315712975627 18.65852337172092 >100 1.6426072631863347 31.08812868933712 >500 0.0553577753474667 4.4565766853317035 >1k 0.04164080446490857 9.989135262031471 >5k 0.0034292427206395297 2.803356852346752 >10k+ 0.0019595672689368742 3.6175981893600295 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 22399 1.1883855591975068 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 16890 0.8961039374456846 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 14804 0.785430591471043 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 10107 0.5362298694945846 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 9341 0.4955895133025542 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 8735 0.4634380043569008 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 8238 0.43706952259784193 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 6491 0.34438192172646176 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 5926 0.3144056798876926 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 5570 0.29551799476450347 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 5449 0.28909830403443076 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 4827 0.2560979103641397 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 4708 0.24978433022464674 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 4095 0.2172614342119644 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 4015 0.21301701058877584 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 3962 0.21020507993841342 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 3908 0.20734009399276115 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 3876 0.20564232454348572 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 3817 0.20251206212138417 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC 3549 0.18829324298370248 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 3523 0.1869138053061662 No Hit CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG 3446 0.18282854756884723 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 3396 0.18017578280435437 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 3302 0.1751885850471078 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3041 0.16134115297645513 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA 3006 0.15948421764131013 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT 2992 0.15874144350725214 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 2973 0.15773339289674485 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2935 0.15571729167573028 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 2900 0.15386035634058529 No Hit TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG 2653 0.1407556984039906 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 2601 0.13799682304891803 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT 2557 0.13566239005616434 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 2549 0.13523794769384548 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC 2545 0.13502572651268605 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 2538 0.13465433944565705 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA 2408 0.12775715105797564 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG 2387 0.12664298985688865 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC 2371 0.12579410513225095 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 2234 0.11852552967754053 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG 2227 0.11815414261051153 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 2176 0.11544832255072882 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 2155 0.11433416134964182 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2135 0.11327305544384467 No Hit CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT 2078 0.11024890361232284 No Hit ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC 2066 0.10961224006884454 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA 1998 0.10600447998913429 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG 1948 0.10335171522464143 No Hit ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT 1937 0.102768106976453 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 1924 0.10207838813768486 No Hit ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC 1887 0.10011534221196015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.04950059050543658 0.0 0.0 0.0 0.0 2 0.10043367398369929 0.0 0.0 0.0 0.0 3 0.13948237131703403 0.0 0.0 0.0 0.0 4 0.16860972843116553 0.0 0.0 0.0 0.0 5 0.19805541731703616 0.0 0.0 0.0 0.0 6 0.24108326179711018 0.0 0.0 0.0 0.0 7 0.2733408813333432 0.0 0.0 0.0 0.0 8 0.3089940397681271 0.0 0.0 0.0 0.0 9 0.34215359932428774 0.0 0.0 0.0 0.0 10 0.3739337212029121 0.0 0.0 0.0 0.0 11 0.4053424560145074 0.0 0.0 0.0 0.0 12 0.4406772826775522 0.0 0.0 0.0 0.0 13 0.47839959762864054 0.0 0.0 0.0 0.0 14 0.5103388853931344 0.0 0.0 0.0 0.0 15 0.5406865142989327 0.0 0.0 0.0 0.0 16 0.5679569360779191 0.0 0.0 0.0 0.0 17 0.5996840026612537 0.0 0.0 0.0 0.0 18 0.6383082576322695 0.0 0.0 0.0 0.0 19 0.6628728593514733 0.0 0.0 0.0 0.0 20 0.6895596728822714 0.0 0.0 0.0 0.0 21 0.7121612286757505 0.0 0.0 0.0 0.0 22 0.734975005650389 0.0 0.0 0.0 0.0 23 0.7599109944366217 0.0 0.0 0.0 0.0 24 0.7865978079674198 0.0 0.0 0.0 0.0 25 0.8133376767935078 0.0 0.0 0.0 0.0 26 0.8378492232174216 0.0 0.0 0.0 0.0 27 0.8626260461177849 0.0 0.0 0.0 0.0 28 0.8917003479366266 0.0 0.0 0.0 0.0 29 0.9187585485344536 0.0 0.0 0.0 0.0 30 0.9444903667500342 0.0 0.0 0.0 0.0 31 0.9725566179583686 0.0 0.0 0.0 0.0 32 1.0007289797572827 0.0 0.0 0.0 0.0 33 1.0277341250598198 0.0 0.0 0.0 0.0 34 1.052882335027212 0.0 0.0 0.0 0.0 35 1.075590001411271 0.0 0.0 0.0 0.0 36 1.0959632348025758 0.0 0.0 0.0 0.0 37 1.114479532858736 0.0 0.0 0.0 0.0 38 1.125568089574316 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTTA 10 0.0016534487 235.86708 44 CGTATCG 15 1.76684E-5 235.86707 44 TTGCGTC 15 1.76684E-5 235.86707 44 GTTTCGC 30 6.019036E-9 196.5559 44 CTATGCG 25 5.7332545E-7 188.69366 44 CTATCAT 3330 0.0 139.89114 44 CCAGAAT 870 0.0 139.62247 44 AATCGCA 415 0.0 139.24683 44 ACAACGG 20 4.9984847E-6 122.15199 43 CCGTATC 15 2.4341737E-4 122.15199 43 CAATCGT 40 1.8189894E-12 122.15199 43 CTGGACG 70 6.002665E-11 117.93354 44 CGTGCGC 50 1.2714372E-7 117.93353 44 ACGCTTA 120 0.0 117.93353 44 CGGACTT 60 2.733941E-9 117.93353 44 TAATTCC 235 0.0 110.40586 44 TAGTGCA 55 2.244251E-7 107.21231 44 ACTGTAA 110 0.0 105.4949 43 ATTAGAC 45 1.0681582E-5 104.82982 44 ACTTCTA 2000 0.0 103.19185 44 >>END_MODULE