FastQCFastQC Report
Tue 24 May 2016
ERR840996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159616
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC466021.4749260669650996No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA309050.9781251898964937No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT178740.5657016548846442No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT167210.5292098786688002No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA137090.4338818388057283No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT118590.37533041989912697No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG100460.3179500293706577No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC97900.3098477789706091No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGG89390.2829141262735725No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA89130.28209124146731757No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG78350.2479731714233628No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA76910.2434156555733355No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA64940.20553130506998318No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA61830.19568833681054915No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC56150.17771146873544127No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA51560.16318438696347912No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT51420.16274129514472643No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC50500.15982954890720896No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG49710.15732924507281898No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT49440.15647471085093886No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG47990.15188554558528633No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC47220.14944854058214668No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG45620.1443846340821163No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG41300.13071208653203426No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA40320.12761044380076567No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG38100.12058427353197351No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA34070.10782955903502198No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC33270.10529760578500678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTA100.0013646209251.5025244
CGCTATA150.004591892167.6683544
CTATACG650.0154.7707744
CTATCAT35200.0130.0382244
TCGTCTA152.4492983E-4121.9680743
TAATCGT15350.0114.4195543
TCGTAGG1150.0109.3489144
ACTTCTA19300.0107.50754544
TCGTCGG602.5735062E-7104.7927244
CCAGAAT14700.0100.0877444
TCGCAAA800.099.0990543
TATCCAC706.445389E-789.8223344
CCTTAGC68900.085.767143
AATACCG454.905511E-583.83417544
ACGATCT150.001169213482.3219442
AATCGTT203.550145E-582.3219342
ACCACTC6250.078.4687944
AACGCTT4150.077.88322443
TTAATCG16550.076.8503942
GAACACA23300.076.6381144
Produced by FastQC (version 0.11.5)