##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840996.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3159616
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.53166302487391	38.0	35.0	39.0	27.0	39.0
2	36.703546886710285	39.0	35.0	39.0	33.0	39.0
3	36.94862382010979	39.0	35.0	39.0	33.0	39.0
4	37.41016281725374	39.0	35.0	40.0	33.0	41.0
5	38.027232739674695	40.0	35.0	41.0	34.0	41.0
6	38.05188320352853	40.0	35.0	41.0	34.0	41.0
7	38.08400799337641	40.0	35.0	41.0	34.0	41.0
8	38.03231342036501	40.0	35.0	41.0	34.0	41.0
9	38.052872247766814	40.0	35.0	41.0	34.0	41.0
10	38.077496442605685	40.0	35.0	41.0	34.0	41.0
11	37.95111874354352	39.0	35.0	41.0	34.0	41.0
12	37.9197943674168	39.0	35.0	41.0	34.0	41.0
13	37.8521940640888	39.0	35.0	41.0	34.0	41.0
14	37.760347143450346	39.0	35.0	40.0	34.0	41.0
15	37.7115465930037	39.0	35.0	40.0	33.0	41.0
16	37.66034511788774	39.0	35.0	40.0	34.0	41.0
17	37.73376923018493	39.0	35.0	40.0	34.0	41.0
18	37.74537000698819	39.0	35.0	40.0	34.0	41.0
19	37.756949262188826	39.0	35.0	41.0	34.0	41.0
20	37.65295909376329	39.0	35.0	41.0	33.0	41.0
21	37.568749177115194	39.0	35.0	41.0	33.0	41.0
22	37.51192043590107	39.0	35.0	40.0	33.0	41.0
23	37.40842779628917	38.0	35.0	40.0	33.0	41.0
24	37.24089731157204	38.0	35.0	40.0	33.0	41.0
25	37.170896427535936	38.0	35.0	40.0	33.0	41.0
26	36.99337798140421	38.0	35.0	40.0	33.0	41.0
27	36.84679992880613	38.0	35.0	40.0	32.0	41.0
28	36.67792962430883	38.0	35.0	40.0	32.0	41.0
29	36.67316602726829	38.0	35.0	40.0	32.0	41.0
30	36.61206844664968	37.0	35.0	40.0	32.0	41.0
31	36.54536246135481	37.0	35.0	40.0	32.0	41.0
32	36.440140348951346	37.0	34.0	40.0	32.0	41.0
33	36.31690312141864	37.0	34.0	40.0	31.0	41.0
34	36.223685291212874	37.0	34.0	40.0	31.0	41.0
35	36.08388929738325	36.0	34.0	40.0	31.0	41.0
36	36.084086652678906	36.0	34.0	40.0	31.0	41.0
37	35.91276064752461	36.0	34.0	39.0	31.0	41.0
38	35.748996000004176	36.0	34.0	39.0	31.0	41.0
39	35.66296548620026	36.0	34.0	39.0	31.0	41.0
40	35.44079676074008	35.0	34.0	39.0	30.0	41.0
41	35.24242092711474	35.0	34.0	39.0	30.0	40.0
42	35.05458776334199	35.0	33.0	39.0	29.0	40.0
43	34.91672448090486	35.0	33.0	38.0	29.0	40.0
44	34.643960456751444	35.0	33.0	38.0	29.0	40.0
45	34.44162214161904	35.0	33.0	37.0	29.0	40.0
46	34.20320830393002	35.0	32.0	37.0	28.0	40.0
47	34.21502813462151	35.0	33.0	37.0	29.0	39.0
48	34.998165398226945	35.0	34.0	37.0	30.0	40.0
49	35.365385010482896	35.0	34.0	38.0	31.0	40.0
50	35.71986368062318	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	34.0
22	107.0
23	257.0
24	642.0
25	1339.0
26	2666.0
27	5713.0
28	11506.0
29	22203.0
30	41982.0
31	79244.0
32	144506.0
33	242026.0
34	367803.0
35	230088.0
36	348431.0
37	491784.0
38	429322.0
39	466963.0
40	272993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.498994814559744	19.546172699467277	31.95483248597298
2	27.496347657436853	22.187126536895622	29.29270518949138	21.023820616176142
3	25.198346887723066	24.411130972877718	24.550894792278555	25.839627347120665
4	21.188682422167755	25.880170248536533	29.503268751645766	23.427878577649942
5	17.58871964188053	28.454027324839476	28.178455862990948	25.778797170289046
6	23.07837408090097	26.781672203204437	27.43181449897709	22.708139216917502
7	24.960691425793513	26.326142164111083	23.936073244343618	24.777093165751786
8	21.973872774413095	24.398439557212015	29.492824444489457	24.134863223885432
9	22.20102063035508	29.324924294597825	26.53540810022484	21.938646974822255
10	28.81432427231664	23.32913240089935	26.69213600640078	21.164407320383237
11	22.95171311956896	23.924679454718547	25.643590866738236	27.480016558974256
12	22.789225019749235	26.050159259859424	26.161027162794465	24.999588557596873
13	23.911544947234095	24.175880866535678	25.01114059430007	26.901433591930157
14	20.41906358241001	25.975846431971476	25.884031477242804	27.7210585083757
15	21.947002420547307	29.907146944438818	25.601750339281736	22.54410029573214
16	21.47868601754137	33.111302132917416	24.604382304685128	20.80562954485608
17	23.187374668314124	34.19798481840831	21.243309313536834	21.37133119974073
18	22.36566089043732	27.00188883712451	24.301434098320808	26.331016174117362
19	24.401889343515162	24.99493609349997	25.055069983187835	25.548104579797037
20	23.943700753509287	23.781339251352062	25.916345530596125	26.35861446454253
21	23.91664050314975	30.314664820028764	23.5959369746197	22.172757702201785
22	25.00927327877818	27.158806639794204	22.090184376835666	25.74173570459195
23	26.46881772974944	25.467398569952802	25.583108833478498	22.480674866819257
24	23.404901101906052	27.071201057343675	24.102074429297737	25.421823411452532
25	24.079449762433004	25.37617824733511	25.902870759033643	24.641501231198248
26	23.960470107006266	31.544242833799046	24.63134304814434	19.86394401105035
27	25.39374684785271	26.503882029308574	25.01251203327702	23.0898590895617
28	23.915715061298755	25.580982538965007	24.523331949829096	25.979970449907142
29	24.34262381635018	28.431722223766005	25.095059768901212	22.130594190982602
30	25.47594247018771	28.189331744967262	26.97733651138108	19.357389273463948
31	24.556388323844917	31.264724052684066	22.914273237874227	21.26461438559679
32	22.45288708328467	27.785384177476363	26.053478921050704	23.708249818188268
33	22.244143565757675	24.361558674998726	30.743222385770142	22.651075373473457
34	22.949291864502268	29.46383299337852	25.244105658635586	22.342769483483625
35	20.95647787624522	27.651542964594412	24.70990544370627	26.682073715454095
36	22.32968387381334	25.46940760862844	24.942324524340492	27.258583993217723
37	19.635731983511548	31.477535745773427	24.009277605490254	24.877454665224775
38	24.47411553541042	26.40891389867211	24.98628339258931	24.130687173328166
39	22.473427684246104	30.035885104616728	25.31768562967062	22.173001581466544
40	20.60853385625643	27.224094962532636	26.278051075420734	25.889320105790205
41	21.85064442165268	31.49822421440286	23.798342315014313	22.852789048930145
42	21.302187068855698	31.49605787722634	24.882956106690475	22.318798947227485
43	20.422802008610944	24.858405349963377	26.031605116879746	28.687187524545934
44	20.103531822294677	28.496414336670977	22.721552117373456	28.678501723660894
45	19.68988165313836	27.537371647911336	29.45226644805305	23.32048025089725
46	19.337820155483076	28.19744473037966	28.56326612383396	23.901468990303307
47	23.187599532859117	29.48911774073681	25.009714407049582	22.313568319354495
48	24.73352244855553	28.063028763136472	23.306675714738493	23.896773073569506
49	18.040270373656863	29.18706910299068	24.148820955230246	28.623839568122207
50	19.561433141903304	30.380144268028538	26.51153548079407	23.54688710927409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40269.0
1	34281.0
2	31311.5
3	32700.5
4	28119.0
5	18318.5
6	8645.5
7	6007.0
8	6035.5
9	5605.5
10	5169.0
11	4857.0
12	4538.5
13	4340.0
14	4677.0
15	4817.5
16	4540.5
17	4803.5
18	5408.0
19	5679.5
20	5868.5
21	7189.5
22	9008.0
23	11158.5
24	13552.5
25	15634.5
26	18900.5
27	24107.0
28	28622.0
29	32324.0
30	37764.0
31	49700.0
32	70893.0
33	85323.5
34	102344.5
35	127580.5
36	129657.0
37	118583.5
38	120943.5
39	139291.5
40	165532.5
41	184362.0
42	198216.5
43	221343.0
44	239975.5
45	245105.0
46	248454.5
47	248401.0
48	270861.5
49	311595.0
50	356453.5
51	382303.5
52	342819.0
53	282936.0
54	245540.5
55	228908.5
56	218480.0
57	198507.5
58	171500.0
59	145930.5
60	128721.0
61	110028.5
62	90927.5
63	75095.0
64	62659.5
65	53167.5
66	40361.5
67	27417.5
68	17784.5
69	12363.0
70	9119.5
71	6817.5
72	5478.0
73	4503.5
74	3552.5
75	2591.5
76	2068.0
77	1480.0
78	865.5
79	529.0
80	258.0
81	108.0
82	55.0
83	28.0
84	13.5
85	16.5
86	21.0
87	22.0
88	22.0
89	22.0
90	12.0
91	2.0
92	2.0
93	2.0
94	1.0
95	1.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	17555.0
25	16730.0
26	18322.0
27	25462.0
28	22682.0
29	18424.0
30	31334.0
31	21126.0
32	23848.0
33	17663.0
34	18373.0
35	16981.0
36	17697.0
37	19166.0
38	19308.0
39	23681.0
40	25182.0
41	35113.0
42	33754.0
43	62675.0
44	156856.0
45	291461.0
46	342423.0
47	363034.0
48	498625.0
49	518911.0
50	503230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.109362602683888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50321558717756	9.76689668198241
2	10.929763322871652	2.8656446112207927
3	3.7757126156207153	1.4849155728509993
4	1.8442385642189922	0.9670716825679955
5	1.0119959733452666	0.663331108351956
6	0.6613256455512762	0.5201734611591408
7	0.46030572082319976	0.42240202216527734
8	0.3625478425338716	0.38022169028778113
9	0.2928160680506899	0.345476881077179
>10	2.9988023035858626	9.412366726446459
>50	1.510421756342492	14.631415051006973
>100	1.5431117497547184	33.64486067847031
>500	0.0610439797243974	5.397779823323461
>1k	0.03869454452475464	9.679148560015406
>5k	0.003669310256657768	3.3587662648646135
>10k+	0.002335015617873125	6.459529184209213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	46602	1.4749260669650996	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	30905	0.9781251898964937	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	17874	0.5657016548846442	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	16721	0.5292098786688002	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	13709	0.4338818388057283	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT	11859	0.37533041989912697	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	10046	0.3179500293706577	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	9790	0.3098477789706091	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGG	8939	0.2829141262735725	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	8913	0.28209124146731757	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	7835	0.2479731714233628	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	7691	0.2434156555733355	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	6494	0.20553130506998318	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6183	0.19568833681054915	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	5615	0.17771146873544127	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA	5156	0.16318438696347912	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	5142	0.16274129514472643	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5050	0.15982954890720896	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	4971	0.15732924507281898	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	4944	0.15647471085093886	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4799	0.15188554558528633	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	4722	0.14944854058214668	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4562	0.1443846340821163	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	4130	0.13071208653203426	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4032	0.12761044380076567	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	3810	0.12058427353197351	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	3407	0.10782955903502198	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC	3327	0.10529760578500678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04722092811278333	0.0	0.0	0.0	0.0
2	0.08124404990986246	0.0	0.0	0.0	0.0
3	0.10365183617249692	0.0	0.0	0.0	0.0
4	0.12637611659138326	0.0	0.0	0.0	0.0
5	0.1469165873321315	0.0	0.0	0.0	0.0
6	0.1819524904292167	0.0	0.0	0.0	0.0
7	0.20375893779497256	0.0	0.0	0.0	0.0
8	0.23015455042638092	0.0	0.0	0.0	0.0
9	0.253163675585894	0.0	0.0	0.0	0.0
10	0.2719634284672568	0.0	0.0	0.0	0.0
11	0.2922507038830035	0.0	0.0	0.0	0.0
12	0.3174436387206547	0.0	0.0	0.0	0.0
13	0.34222513115517833	0.0	0.0	0.0	0.0
14	0.36669012943345014	0.0	0.0	0.0	0.0
15	0.3912184265429723	0.0	0.0	0.0	0.0
16	0.41565177540561893	0.0	0.0	0.0	0.0
17	0.44242718102452955	0.0	0.0	0.0	0.0
18	0.4720193846340821	0.0	0.0	0.0	0.0
19	0.49540830278109743	0.0	0.0	0.0	0.0
20	0.5183224796937349	0.0	0.0	0.0	0.0
21	0.5460157183657761	0.0	0.0	0.0	0.0
22	0.5699426765784197	0.0	0.0	0.0	0.0
23	0.5944709736879418	0.0	0.0	0.0	0.0
24	0.6155178350786931	0.0	0.0	0.0	0.0
25	0.6366279953006948	0.0	0.0	0.0	0.0
26	0.6623906196195993	0.0	0.0	0.0	0.0
27	0.6832159351009743	0.0	0.0	0.0	0.0
28	0.7070479450667423	0.0	0.0	0.0	0.0
29	0.7339182989325285	0.0	0.0	0.0	0.0
30	0.7627825659827017	0.0	0.0	0.0	0.0
31	0.7894313739391116	0.0	0.0	0.0	0.0
32	0.8130418379955032	0.0	0.0	0.0	0.0
33	0.8399438412769147	0.0	0.0	0.0	0.0
34	0.8663078044926978	0.0	0.0	0.0	0.0
35	0.8882724989365797	0.0	0.0	0.0	0.0
36	0.9068507059085661	0.0	0.0	0.0	0.0
37	0.9251124187243007	0.0	0.0	0.0	0.0
38	0.9368227025056209	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACTA	10	0.0013646209	251.50252	44
CGCTATA	15	0.004591892	167.66835	44
CTATACG	65	0.0	154.77077	44
CTATCAT	3520	0.0	130.03822	44
TCGTCTA	15	2.4492983E-4	121.96807	43
TAATCGT	1535	0.0	114.41955	43
TCGTAGG	115	0.0	109.34891	44
ACTTCTA	1930	0.0	107.507545	44
TCGTCGG	60	2.5735062E-7	104.79272	44
CCAGAAT	1470	0.0	100.08774	44
TCGCAAA	80	0.0	99.09905	43
TATCCAC	70	6.445389E-7	89.82233	44
CCTTAGC	6890	0.0	85.7671	43
AATACCG	45	4.905511E-5	83.834175	44
ACGATCT	15	0.0011692134	82.32194	42
AATCGTT	20	3.550145E-5	82.32193	42
ACCACTC	625	0.0	78.46879	44
AACGCTT	415	0.0	77.883224	43
TTAATCG	1655	0.0	76.85039	42
GAACACA	2330	0.0	76.63811	44
>>END_MODULE