FastQCFastQC Report
Tue 24 May 2016
ERR840995.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840995.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8866527
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG853600.9627219315973436No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC740660.8353439853056331No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA703930.7939185207466238No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA644880.7273197273295395No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA473970.5345610519203291No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG409510.46186065863217923No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG374450.4223186823882677No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT279990.3157831696672215No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA257540.2904632219582707No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC256360.28913237392724345No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG199600.2251163279602036No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT173180.1953188661129662No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG164810.18587886779118815No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG156840.17689000439518202No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT143850.16223939768073792No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA140530.15849497779683072No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCT132760.14973168186371055No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG122810.13850970058513326No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG99530.11225364790520573No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG93150.1050580458391431No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC89990.1014940799255447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT37700.0148.30144
GAACACA48550.0112.2472344
AATCGCA9150.090.96831544
CGCCCCA5250.090.9085843
CAATGAA112100.088.1026643
TCGTGCG24700.087.8537643
CCTTAGC115750.087.57854543
ACTTCTA31500.086.2523644
TCGTCGG559.9826E-485.6631344
TCCGGGC15650.077.664443
CCCATAC17900.075.4537444
CGACGTA654.3946784E-968.5310843
ACCTTAG103100.066.5709142
CCCTATC39100.063.46638542
GCCCCAC20100.063.2884344
AACGACA9850.062.0208843
TCCAGAA151800.061.4980443
GCAATGA111000.061.37439342
CCTATCA39100.059.7299643
TACCGCT1603.384539E-758.89340244
Produced by FastQC (version 0.11.5)