##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840995.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8866527
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.039683858178066	39.0	37.0	39.0	33.0	39.0
2	37.714015758368525	39.0	37.0	39.0	35.0	39.0
3	38.0545582278157	39.0	38.0	39.0	35.0	40.0
4	38.45840710799166	39.0	38.0	41.0	35.0	41.0
5	38.98143376769732	40.0	38.0	41.0	35.0	41.0
6	39.03914035337624	40.0	38.0	41.0	35.0	41.0
7	39.076748088625905	40.0	38.0	41.0	35.0	41.0
8	38.97576300168036	40.0	38.0	41.0	35.0	41.0
9	39.026770233711574	40.0	38.0	41.0	35.0	41.0
10	39.01540794947108	40.0	38.0	41.0	35.0	41.0
11	38.898795887047996	40.0	38.0	41.0	35.0	41.0
12	38.819909080522734	40.0	38.0	41.0	35.0	41.0
13	38.776042073745444	40.0	38.0	41.0	35.0	41.0
14	38.758561272074175	40.0	38.0	41.0	35.0	41.0
15	38.76443369540295	40.0	38.0	41.0	35.0	41.0
16	38.726230687618724	40.0	38.0	41.0	35.0	41.0
17	38.71949377698844	40.0	38.0	41.0	35.0	41.0
18	38.67694882111113	40.0	38.0	41.0	35.0	41.0
19	38.60416011816126	40.0	38.0	41.0	35.0	41.0
20	38.53768177776936	40.0	38.0	41.0	35.0	41.0
21	38.43578562384122	40.0	37.0	41.0	35.0	41.0
22	38.340410174130184	40.0	37.0	41.0	34.0	41.0
23	38.238280557877964	40.0	37.0	41.0	34.0	41.0
24	38.12548554806183	40.0	37.0	41.0	34.0	41.0
25	38.00235780311001	39.0	36.0	41.0	34.0	41.0
26	37.90207379763231	39.0	36.0	41.0	33.0	41.0
27	37.73951695827371	39.0	36.0	41.0	33.0	41.0
28	37.5878500827893	39.0	36.0	41.0	33.0	41.0
29	37.57506720360177	39.0	35.0	41.0	33.0	41.0
30	37.5713183711823	39.0	35.0	41.0	33.0	41.0
31	37.456381326765104	39.0	35.0	40.0	33.0	41.0
32	37.42545888583166	39.0	35.0	40.0	33.0	41.0
33	37.28029804968551	38.0	35.0	40.0	32.0	41.0
34	37.14661100708384	38.0	35.0	40.0	32.0	41.0
35	37.08011410040098	38.0	35.0	40.0	32.0	41.0
36	37.064451997132274	38.0	35.0	40.0	32.0	41.0
37	36.98930089651365	38.0	35.0	40.0	32.0	41.0
38	36.83570977213891	38.0	35.0	40.0	31.0	41.0
39	36.7782806960944	38.0	35.0	40.0	31.0	41.0
40	36.45094444186896	38.0	34.0	40.0	31.0	41.0
41	36.39804353890693	37.0	34.0	40.0	31.0	41.0
42	36.25631038829982	37.0	34.0	40.0	31.0	41.0
43	36.14358424831972	37.0	34.0	40.0	30.0	41.0
44	35.88613902607436	37.0	34.0	40.0	29.0	41.0
45	35.668433760227785	37.0	34.0	40.0	29.0	41.0
46	35.40808073196109	36.0	33.0	40.0	29.0	41.0
47	35.7492305810416	37.0	34.0	40.0	30.0	41.0
48	36.57522416761485	37.0	35.0	40.0	31.0	41.0
49	37.08241251400538	38.0	35.0	40.0	32.0	41.0
50	37.18364208876036	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	44.0
22	145.0
23	339.0
24	765.0
25	1551.0
26	3078.0
27	6409.0
28	13818.0
29	28391.0
30	57943.0
31	112965.0
32	205720.0
33	348798.0
34	552971.0
35	567973.0
36	909477.0
37	1456172.0
38	1390852.0
39	1421205.0
40	1787896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.24274397404982	17.39124011013557	30.366015915814614
2	29.640094706754965	23.545543818904516	27.63452928074318	19.179832193597335
3	29.836169223868602	20.52660528750434	23.473373509154147	26.16385197947291
4	22.53147145438118	24.150030784319497	29.34060878628126	23.977888975018065
5	19.301322829107722	28.45795202563529	28.208835319624022	24.03188982563297
6	24.700212383044683	29.68320064891248	25.994980898383325	19.62160606965952
7	28.436162208720507	26.100084057715044	23.72710306978144	21.73665066378301
8	22.847096726824383	25.02517614845136	28.72350132131781	23.404225803406455
9	23.539532446018605	25.945739521235318	28.592627079351363	21.922100953394715
10	27.28815916310862	23.30974687157666	28.575878695232078	20.82621527008264
11	24.89530568169476	23.351454295464276	28.437481778378388	23.31575824446257
12	23.521261481524842	25.966356387343094	28.45037295888232	22.062009172249745
13	22.584502364905674	26.3778139963934	27.961929174749027	23.075754463951895
14	18.893440464344156	29.10102230557692	26.60466719381783	25.400870036261097
15	20.143569178777664	30.090846167840013	28.194444115491894	21.571140537890425
16	22.090193826737345	32.780726884382126	24.74817930402738	20.380899984853144
17	22.711305114167025	33.15873283868645	20.200299395693488	23.929662651453043
18	23.506114626392048	27.688485017865506	24.783807684790222	24.021592670952224
19	24.56359744914779	24.354868597366252	27.29712546975834	23.78440848372762
20	26.053233695673626	25.291458538388255	24.56341699517748	24.09189077076064
21	25.233916278606046	26.648675405826882	25.365704068797175	22.7517042467699
22	25.021262552970292	29.66539209771763	22.76256532010786	22.55078002920422
23	27.110107486279578	26.263981376247997	24.71338552287722	21.912525614595207
24	25.316225845813133	26.366208550427917	23.405985229616963	24.911580374141984
25	21.940205070506302	26.784190015018318	27.917340783409404	23.358264131065972
26	23.50142347567559	30.64803287624953	26.89530683865921	18.95523680941567
27	26.102084770208617	26.746273846410755	23.875771623471763	23.27586975990886
28	26.34376153366842	26.826714484124608	24.37671020429952	22.45281377790745
29	27.238761534450447	28.233351716770205	24.197632352074034	20.330254396705318
30	26.53372266882933	28.68807361608696	24.512227466314027	20.265976248769682
31	23.989008669733046	29.06036034867584	25.560290193757275	21.39034078783384
32	24.012190751496448	29.337878702614965	25.909469749698637	20.740460796189947
33	25.00657501095438	26.504974426275922	26.51847598932316	21.96997457344654
34	21.79010371179693	30.197743666416283	25.366860864221046	22.64529175756574
35	22.00424137934771	28.78671578700729	25.73970385477159	23.469338978873413
36	23.8213896671192	27.697412433654705	25.84628157643755	22.634916322788545
37	22.168204658483564	29.7027665845047	23.559807201262092	24.56922155574964
38	23.522473584745892	29.849665425544153	24.807701061602717	21.82015992810724
39	24.62333111978729	30.644028698089176	24.34882188041659	20.383818301706942
40	20.28714005733803	29.91116421154494	26.291103035569733	23.5105926955473
41	21.42292452488656	33.668450358097445	24.330483109924074	20.578142007091923
42	23.0324516214824	32.527063644316094	23.20135669607278	21.239128038128726
43	20.329083236174743	27.909319274670764	26.2537675847248	25.50782990442969
44	20.755182128278868	29.83406296714959	24.264075334135104	25.146679570436437
45	21.09295192733857	29.02732127276194	25.967408312817792	23.912318487081695
46	20.402440019331376	30.32222311453233	28.215920667919132	21.059416198217164
47	25.478553241680956	29.39844810047898	24.860021256677108	20.262977401162953
48	23.025484825391565	31.04667272347153	23.502385950781875	22.425456500355025
49	19.94321704793917	33.19822906808515	22.507007219339563	24.351546664636114
50	19.605666987010572	34.491915376064554	25.125886297104948	20.77653133981993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71730.0
1	65294.0
2	63123.5
3	64152.5
4	55860.0
5	41849.5
6	27590.0
7	22180.0
8	21007.0
9	19387.5
10	18446.0
11	17852.5
12	17417.5
13	16612.5
14	15568.0
15	15843.0
16	17609.5
17	19803.0
18	22286.5
19	26141.5
20	31238.0
21	38457.0
22	46819.0
23	56565.5
24	66244.0
25	74706.0
26	86502.5
27	102860.0
28	123482.5
29	146593.0
30	170292.0
31	197227.0
32	238068.5
33	275364.0
34	317795.5
35	367001.5
36	388249.5
37	399150.0
38	419348.0
39	451733.0
40	490720.0
41	531483.5
42	601156.5
43	662399.5
44	678950.0
45	695015.0
46	677791.5
47	639818.5
48	669057.0
49	732412.0
50	823942.0
51	872864.0
52	813703.5
53	761160.5
54	682628.0
55	623974.0
56	609815.0
57	565696.5
58	477082.0
59	395418.5
60	368386.5
61	332712.5
62	291202.5
63	234279.5
64	172661.5
65	134793.0
66	103669.5
67	73163.5
68	49900.0
69	35157.5
70	27617.0
71	21651.0
72	16820.5
73	12378.0
74	8449.0
75	5488.0
76	4008.5
77	3118.5
78	2350.0
79	1607.0
80	1328.5
81	1168.5
82	848.5
83	533.5
84	239.5
85	116.0
86	67.0
87	34.0
88	18.5
89	19.0
90	17.0
91	14.0
92	12.5
93	12.0
94	6.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42635.0
25	38705.0
26	42257.0
27	50986.0
28	42854.0
29	37494.0
30	130366.0
31	40405.0
32	49204.0
33	44451.0
34	41853.0
35	45123.0
36	41468.0
37	46554.0
38	44941.0
39	81262.0
40	63086.0
41	64234.0
42	94671.0
43	185900.0
44	510555.0
45	864130.0
46	957766.0
47	1153823.0
48	1393934.0
49	1628875.0
50	1128995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.461057039731498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94188771738214	7.316660768780305
2	12.800221392146113	2.6780769220886365
3	4.600962510126283	1.4439279376829182
4	2.1193312234509416	0.8868177925841696
5	1.1675311750526436	0.6106805108945221
6	0.755112266815392	0.473956349673406
7	0.5757444293508795	0.42160267210230495
8	0.43066196029202425	0.36041434651659576
9	0.3651389769322284	0.3437765698605521
>10	3.4157487693804507	8.206746757898538
>50	1.7194631012249426	13.428382438784976
>100	1.9831633835312736	34.36224884100956
>500	0.07015351172490872	4.964650992140481
>1k	0.04653220271373692	9.20194340900896
>5k	0.0051505109873983615	3.726604911223342
>10k+	0.0031968688887300174	11.573508779750737
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	85360	0.9627219315973436	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	74066	0.8353439853056331	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	70393	0.7939185207466238	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	64488	0.7273197273295395	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	47397	0.5345610519203291	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	40951	0.46186065863217923	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	37445	0.4223186823882677	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	27999	0.3157831696672215	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	25754	0.2904632219582707	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	25636	0.28913237392724345	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	19960	0.2251163279602036	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	17318	0.1953188661129662	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	16481	0.18587886779118815	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	15684	0.17689000439518202	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	14385	0.16223939768073792	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	14053	0.15849497779683072	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCT	13276	0.14973168186371055	No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG	12281	0.13850970058513326	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	9953	0.11225364790520573	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	9315	0.1050580458391431	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	8999	0.1014940799255447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04280142608261386	0.0	0.0	0.0	0.0
2	0.07441470600608333	0.0	0.0	0.0	0.0
3	0.09138865758825299	0.0	0.0	0.0	0.0
4	0.1115431103971149	0.0	0.0	0.0	0.0
5	0.13129154177278207	0.0	0.0	0.0	0.0
6	0.16337851336831208	0.0	0.0	0.0	0.0
7	0.18386003899835865	0.0	0.0	0.0	0.0
8	0.20667618786927508	0.0	0.0	0.0	0.0
9	0.22798103473885548	0.0	0.0	0.0	0.0
10	0.24833849826431476	0.0	0.0	0.0	0.0
11	0.26775985681879727	0.0	0.0	0.0	0.0
12	0.2897301277038913	0.0	0.0	0.0	0.0
13	0.3101101479756392	0.0	0.0	0.0	0.0
14	0.33080596269542745	0.0	0.0	0.0	0.0
15	0.3534078224766022	0.0	0.0	0.0	0.0
16	0.3754119284811291	0.0	0.0	0.0	0.0
17	0.3994010281590526	0.0	0.0	0.0	0.0
18	0.42426984094223136	0.0	0.0	0.0	0.0
19	0.4470295979474263	0.0	0.0	0.0	0.0
20	0.4710863678642156	0.0	0.0	0.0	0.0
21	0.4933498764510614	0.0	0.0	0.0	0.0
22	0.5161434685756893	0.0	0.0	0.0	0.0
23	0.5411814569560325	0.0	0.0	0.0	0.0
24	0.563850986976073	0.0	0.0	0.0	0.0
25	0.5892724400433225	0.0	0.0	0.0	0.0
26	0.6161939167387637	0.0	0.0	0.0	0.0
27	0.6398108301029253	0.0	0.0	0.0	0.0
28	0.6657398099616681	0.0	0.0	0.0	0.0
29	0.6933943809114888	0.0	0.0	0.0	0.0
30	0.7217707677425446	0.0	0.0	0.0	0.0
31	0.7508125785891139	0.0	0.0	0.0	0.0
32	0.7804070297197538	0.0	0.0	0.0	0.0
33	0.8091894379840043	0.0	0.0	0.0	0.0
34	0.835716171619395	0.0	0.0	0.0	0.0
35	0.8606752113877283	0.0	0.0	0.0	0.0
36	0.8806153751068485	0.0	0.0	0.0	0.0
37	0.8973975943455651	0.0	0.0	0.0	0.0
38	0.9092060510276458	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	3770	0.0	148.301	44
GAACACA	4855	0.0	112.24723	44
AATCGCA	915	0.0	90.968315	44
CGCCCCA	525	0.0	90.90858	43
CAATGAA	11210	0.0	88.10266	43
TCGTGCG	2470	0.0	87.85376	43
CCTTAGC	11575	0.0	87.578545	43
ACTTCTA	3150	0.0	86.25236	44
TCGTCGG	55	9.9826E-4	85.66313	44
TCCGGGC	1565	0.0	77.6644	43
CCCATAC	1790	0.0	75.45374	44
CGACGTA	65	4.3946784E-9	68.53108	43
ACCTTAG	10310	0.0	66.57091	42
CCCTATC	3910	0.0	63.466385	42
GCCCCAC	2010	0.0	63.28843	44
AACGACA	985	0.0	62.02088	43
TCCAGAA	15180	0.0	61.49804	43
GCAATGA	11100	0.0	61.374393	42
CCTATCA	3910	0.0	59.72996	43
TACCGCT	160	3.384539E-7	58.893402	44
>>END_MODULE