##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840994.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1998535
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.297405849784965	39.0	37.0	39.0	34.0	39.0
2	37.83494209508465	39.0	38.0	39.0	35.0	39.0
3	38.107546277648375	39.0	38.0	39.0	35.0	39.0
4	38.72464230048511	39.0	38.0	41.0	35.0	41.0
5	39.27040256988244	40.0	39.0	41.0	35.0	41.0
6	39.2589531832067	40.0	39.0	41.0	35.0	41.0
7	39.2517108782183	40.0	39.0	41.0	35.0	41.0
8	39.20792580565264	40.0	38.0	41.0	35.0	41.0
9	39.21766293810216	40.0	39.0	41.0	35.0	41.0
10	39.148005914332245	40.0	39.0	41.0	35.0	41.0
11	39.080567515705255	40.0	38.0	41.0	35.0	41.0
12	39.00859679715391	40.0	38.0	41.0	35.0	41.0
13	38.904148288621414	40.0	38.0	41.0	35.0	41.0
14	38.854050091692166	40.0	38.0	41.0	35.0	41.0
15	38.814624212235465	40.0	38.0	41.0	35.0	41.0
16	38.77172478840751	40.0	38.0	41.0	35.0	41.0
17	38.73405169286502	40.0	38.0	41.0	35.0	41.0
18	38.67682427378055	40.0	38.0	41.0	35.0	41.0
19	38.61789260633414	40.0	38.0	41.0	35.0	41.0
20	38.532570607970335	40.0	38.0	41.0	35.0	41.0
21	38.43680245780034	40.0	38.0	41.0	34.0	41.0
22	38.35923964303853	40.0	37.0	41.0	34.0	41.0
23	38.206841511407106	40.0	37.0	41.0	34.0	41.0
24	38.01797866937532	39.0	37.0	41.0	34.0	41.0
25	37.919012217490035	39.0	36.0	41.0	33.0	41.0
26	37.78387265703465	39.0	36.0	41.0	33.0	41.0
27	37.67701918699915	39.0	36.0	41.0	33.0	41.0
28	37.502430291306744	39.0	35.0	41.0	33.0	41.0
29	37.46231112969804	39.0	35.0	41.0	33.0	41.0
30	37.34652666807761	38.0	35.0	40.0	33.0	41.0
31	37.24280121404797	38.0	35.0	40.0	32.0	41.0
32	37.137847539974814	38.0	35.0	40.0	32.0	41.0
33	37.01310158635942	38.0	35.0	40.0	32.0	41.0
34	36.92020861262968	38.0	35.0	40.0	32.0	41.0
35	36.73602454657442	38.0	35.0	40.0	31.0	41.0
36	36.689687122032126	38.0	35.0	40.0	31.0	41.0
37	36.65961790830805	38.0	35.0	40.0	31.0	41.0
38	36.418763624294506	37.0	34.0	40.0	31.0	41.0
39	36.2782133035783	37.0	34.0	40.0	31.0	41.0
40	36.11325164344056	37.0	34.0	40.0	31.0	41.0
41	36.01495513179403	37.0	34.0	40.0	31.0	41.0
42	35.848805165820664	36.0	34.0	40.0	30.0	41.0
43	35.64309223844887	36.0	34.0	40.0	30.0	41.0
44	35.40858170190137	36.0	33.0	39.0	29.0	41.0
45	35.15180118357351	36.0	33.0	39.0	29.0	41.0
46	34.86409173733516	35.0	33.0	39.0	28.0	41.0
47	35.023303991872886	35.0	33.0	39.0	29.0	41.0
48	35.95226785841554	36.0	34.0	40.0	31.0	41.0
49	36.41820841500647	37.0	35.0	40.0	32.0	41.0
50	36.871599469807556	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	4.0
21	20.0
22	37.0
23	79.0
24	203.0
25	379.0
26	709.0
27	1497.0
28	3169.0
29	6482.0
30	13222.0
31	25041.0
32	45976.0
33	77495.0
34	122687.0
35	136077.0
36	227974.0
37	350126.0
38	334663.0
39	307648.0
40	345044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.20092968099133	19.53490932107769	39.264160997930986
2	21.659265411914227	18.746832054479906	35.988211364824735	23.60569116878113
3	22.457750302096287	22.340264243558405	29.877335147995908	25.3246503063494
4	19.637234274105783	23.018711205958365	35.29155106115229	22.05250345878356
5	17.543100320985122	24.034004908595545	33.721851256045056	24.70104351437428
6	20.14720782973528	24.10240501167105	27.16344722509238	28.586939933501288
7	24.971041287743272	21.468025328553164	23.44102054755108	30.11991283615248
8	24.409029614192395	22.74476053709342	28.948404706447473	23.89780514226671
9	24.529868128404058	25.69081852456925	25.286972707508248	24.49234063951845
10	26.9825146920119	19.018180817448783	26.644116815567404	27.355187674971916
11	25.39990543072801	21.176912088104537	25.684263723177224	27.738918757990227
12	23.715371509630803	23.6658352243018	26.13234194047139	26.486451325596
13	25.8583412349546	21.888533350679374	24.94922530753777	27.303900106828248
14	23.177777722181496	21.839697578476233	24.232850563037424	30.749674136304844
15	22.472411041087597	29.2523773664209	24.417385735050924	23.857825857440577
16	21.763091464497744	29.71611705574333	22.914334750204524	25.6064567295544
17	22.819315148346163	30.79460704966388	21.39216976435239	24.993908037637567
18	22.293279827473626	25.242890417230623	22.778235057179383	29.68559469811637
19	24.802317697713576	22.608760917371974	22.230183609493952	30.358737775420497
20	24.489238367103905	21.358895390873816	25.20406197539698	28.947804266625305
21	24.90934609601533	27.58370506395935	23.199843885646235	24.30710495437908
22	26.122234536798207	24.549132239365335	22.38164455463627	26.946988669200188
23	27.84409580017363	22.664301600922677	27.33101997212958	22.16058262677411
24	25.536555526923472	25.222625573232392	24.150590307400172	25.090228592443964
25	25.913796416752945	23.970347206920593	24.104062468872716	26.011793907453747
26	27.04140619663579	30.66871753875111	23.749098737853515	18.54077752675958
27	26.991538412186966	25.835236825677445	22.269027370573554	24.90419739156204
28	26.557304412576183	23.230179715401043	22.58914486977249	27.62337100225029
29	24.78566947793964	26.625000968069205	24.822275401490568	23.767054152500588
30	27.70907097880625	25.905337671542384	25.81917322693385	20.566418122717515
31	25.953129787384484	28.602771150910144	22.91146301160364	22.532636050101736
32	23.39285356166133	26.446656090453015	24.908196987784578	25.252293360101074
33	24.507992430407295	23.484778374537406	30.298422363274497	21.708806831780798
34	26.278662160462606	27.70218379639664	25.139266858795793	20.879887184344955
35	21.8656626259096	23.43380213018875	25.231432041714935	29.469103202186712
36	22.320192520234773	24.503207312814425	27.328607948574373	25.847992218376426
37	23.665634803474315	31.732852695610557	22.401119788280468	22.20039271263466
38	27.29496724674267	25.10139744666915	22.09901094559404	25.504624360994143
39	22.334258476064768	30.417907458804226	24.59022741693769	22.657606648193315
40	21.006943863838288	26.968756514401075	25.730365386137464	26.29393423562317
41	23.250859477410753	29.395026835481747	22.87187996771198	24.48223371939552
42	21.957346143552478	30.605434817123232	23.287358980988802	24.149860058335488
43	22.72585523969454	23.93315786420803	24.17281507342194	29.168171822675486
44	23.023939407812797	25.789068378687084	22.436459554926344	28.75053265857378
45	22.94405655834168	25.19270916724346	29.362130008678378	22.501104265736487
46	22.027717974416568	27.30477756212442	27.232259605802	23.435244857657008
47	21.869535855585855	30.667405394764057	25.324557199234803	22.138501550415285
48	26.47759811176	24.99088161187948	23.666828771344363	24.86469150501616
49	20.187450401317616	26.000224105458503	23.066284561743355	30.746040931480522
50	19.86275398063791	29.907216321873793	29.154879951678662	21.075149745809636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24371.0
1	23025.5
2	24279.5
3	25918.5
4	22966.0
5	15528.0
6	7621.0
7	5332.5
8	5510.0
9	5560.0
10	5627.0
11	5316.5
12	4752.5
13	4429.0
14	4681.5
15	4837.0
16	4352.5
17	4267.0
18	4881.5
19	5389.0
20	5740.5
21	6726.5
22	7740.0
23	8342.0
24	8977.5
25	10034.5
26	11363.0
27	14025.5
28	18005.0
29	21064.0
30	23405.5
31	30887.5
32	44336.5
33	50050.5
34	51567.0
35	57049.5
36	57432.5
37	55189.0
38	56696.5
39	60760.5
40	65985.0
41	74969.5
42	83542.0
43	85345.5
44	87084.5
45	92907.5
46	105514.0
47	117222.5
48	137010.5
49	162823.0
50	194467.0
51	221418.5
52	214378.0
53	192696.0
54	176782.5
55	170964.5
56	171399.0
57	171201.5
58	166300.5
59	150985.0
60	130077.0
61	116500.0
62	104766.0
63	87792.5
64	73116.5
65	64830.5
66	51720.5
67	36524.5
68	26665.5
69	19947.5
70	15802.5
71	11928.0
72	8533.5
73	5417.0
74	3208.5
75	2318.5
76	1960.0
77	1561.0
78	1054.5
79	633.0
80	353.5
81	250.5
82	233.5
83	173.5
84	110.5
85	61.5
86	32.5
87	25.0
88	19.5
89	16.5
90	11.5
91	4.5
92	1.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10729.0
25	11411.0
26	12461.0
27	13962.0
28	13127.0
29	13778.0
30	13752.0
31	14497.0
32	14664.0
33	11619.0
34	14427.0
35	12847.0
36	10915.0
37	10527.0
38	12170.0
39	13547.0
40	15114.0
41	15396.0
42	22355.0
43	37519.0
44	98579.0
45	190317.0
46	210951.0
47	249755.0
48	302637.0
49	353474.0
50	298005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.535010463010964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41236061600485	7.891372955048951
2	11.479694097221236	2.6483678304722433
3	4.413163337636701	1.5271765582384715
4	2.280792421537044	1.0523585778554365
5	1.4681695131664119	0.8467675347924136
6	0.9584142173702154	0.6633190815158339
7	0.7441393580499506	0.6008558696731109
8	0.6189268333668422	0.5711461998979813
9	0.48706512059904117	0.5056471136049874
>10	5.038178151883212	13.614242164579684
>50	2.229863733661947	18.60359124718244
>100	1.7719785259909318	34.42789696052572
>500	0.05490899844194589	4.285378206111372
>1k	0.039553092098011874	7.6622377680102005
>5k	9.306609905414558E-4	0.6859245985106995
>10k+	0.0018613219810829115	4.413717333980461
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	34064	1.7044485085325003	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	22558	1.128726792375415	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	13011	0.6510268771875399	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	12596	0.6302616666708364	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7726	0.3865831721736172	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5053	0.2528352017853077	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4593	0.22981834193546774	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3671	0.18368454893209277	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3365	0.16837333346676442	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3319	0.1660716474817804	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	3201	0.16016732256377797	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	2921	0.14615706004648404	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	2777	0.13895178218044718	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	2749	0.13755075592871777	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2727	0.13644994958807327	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	2658	0.13299742061059727	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	2474	0.12379067667066126	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2343	0.1172358752786416	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2315	0.11583484902691221	No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC	2165	0.10832935124979047	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2060	0.10307550280580526	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	2056	0.10287535619841533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05203811792137741	0.0	0.0	0.0	0.0
2	0.10377601593166995	0.0	0.0	0.0	0.0
3	0.1342983735586317	0.0	0.0	0.0	0.0
4	0.15791567323064146	0.0	0.0	0.0	0.0
5	0.18528572179121205	0.0	0.0	0.0	0.0
6	0.2183599486623952	0.0	0.0	0.0	0.0
7	0.2427778347639646	0.0	0.0	0.0	0.0
8	0.2752015851611305	0.0	0.0	0.0	0.0
9	0.3022213771587688	0.0	0.0	0.0	0.0
10	0.32633904334925334	0.0	0.0	0.0	0.0
11	0.3473544371251942	0.0	0.0	0.0	0.0
12	0.37527488885608706	0.0	0.0	0.0	0.0
13	0.4005934346909111	0.0	0.0	0.0	0.0
14	0.4230098547185814	0.0	0.0	0.0	0.0
15	0.45002964671621964	0.0	0.0	0.0	0.0
16	0.4760987423287558	0.0	0.0	0.0	0.0
17	0.5079720895555995	0.0	0.0	0.0	5.003665184747828E-5
18	0.5375437507974591	0.0	0.0	0.0	5.003665184747828E-5
19	0.5638630296692327	0.0	0.0	0.0	5.003665184747828E-5
20	0.5867297795635303	0.0	0.0	0.0	5.003665184747828E-5
21	0.6108474457540148	0.0	0.0	0.0	5.003665184747828E-5
22	0.6329636458706002	0.0	0.0	0.0	5.003665184747828E-5
23	0.655129882639033	0.0	0.0	0.0	5.003665184747828E-5
24	0.6750945067261769	0.0	0.0	0.0	5.003665184747828E-5
25	0.6986617697463392	0.0	0.0	0.0	5.003665184747828E-5
26	0.7298846404991657	0.0	0.0	0.0	5.003665184747828E-5
27	0.753251756911938	0.0	0.0	0.0	5.003665184747828E-5
28	0.7781700095319822	0.0	0.0	0.0	5.003665184747828E-5
29	0.8066909010850448	0.0	0.0	0.0	5.003665184747828E-5
30	0.8338107663863781	0.0	0.0	0.0	5.003665184747828E-5
31	0.8603301918655415	0.0	0.0	0.0	5.003665184747828E-5
32	0.8862491775225353	0.0	0.0	0.0	5.003665184747828E-5
33	0.9108672102314945	0.0	0.0	0.0	5.003665184747828E-5
34	0.934134253340572	0.0	0.0	0.0	5.003665184747828E-5
35	0.953798657516631	0.0	0.0	0.0	5.003665184747828E-5
36	0.9738133182556222	0.0	0.0	0.0	5.003665184747828E-5
37	0.9912761097503922	0.0	0.0	0.0	5.003665184747828E-5
38	1.0038353093641093	0.0	0.0	0.0	5.003665184747828E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACA	10	0.0011238082	268.33252	44
ACGATAA	35	6.9958332E-9	191.6661	44
AATCGCA	385	0.0	191.66609	44
CATGACC	200	0.0	181.12447	44
CTATCAT	2125	0.0	166.68185	44
ACCGTAA	15	2.3736671E-4	122.9277	43
GAACGTT	55	1.04006176E-7	121.96934	44
ACGAACA	120	0.0	111.80522	44
CCCAGTT	180	0.0	104.35154	44
ACATTGG	445	0.0	102.50907	44
AAGTAGC	160	0.0	100.6247	44
CTATGAC	55	1.5250502E-5	97.57547	44
TTAAGCC	105	0.0	93.6592	43
ACTCGAC	20	7.4533577E-4	92.19577	43
AACGATA	35	7.3806586E-7	87.805504	43
ACTTCTA	1475	0.0	86.41217	44
GCGTATT	25	1.016695E-6	83.20913	42
AATACTT	30	3.090281E-8	83.20912	42
GGGGTAG	65	3.4941786E-5	82.56386	44
TAAGCCA	215	0.0	81.123795	44
>>END_MODULE