FastQCFastQC Report
Tue 24 May 2016
ERR840993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193494
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC162031.357610511657369No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT108670.9105198685540103No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA100920.8455844771737436No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT62790.5261023515828316No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC61050.5115233088729395No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA45500.38123358810350116No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA41640.3488915738160393No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA32220.2699636529383474No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA27540.23075105530484444No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA27170.22765091403894783No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC20170.16899959279225535No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG18960.15886129297675564No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA17030.14269028583302473No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC16220.13590349008876457No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT15850.13280334882286798No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG15120.12668685389285575No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT14690.12308398701627322No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13800.1156268904577652No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC13630.11420250122748836No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC13190.11051584674912483No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC12510.10481828982801757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAAC400.0205.6931644
ATTCGTC100.0024903105205.6931644
GACCGCA305.638867E-11205.6931544
GACCCGT153.0492278E-5205.6931544
CTATACG501.2732926E-11143.9852144
CTATCAT17150.0140.327144
CGGCAAA302.8081213E-6137.1287744
CCGGGCT750.0123.41588644
TCAACGA356.04496E-6117.5389544
ACGGGTC251.4706893E-7115.0996943
ACCGTCA206.7155015E-6115.09968643
TGCGTCA451.5330443E-7114.2739844
AATCGCA2100.0112.6414944
ACCGCAC553.8635335E-9112.19626644
TCGTGCG1200.0110.3038643
AAAGGCG455.456968E-12102.3108443
GTGCGTC402.0736479E-10100.7122343
GATTTTC3450.095.3939344
GCGCGAG252.0346466E-592.0797543
TGAACGT252.0346466E-592.0797543
Produced by FastQC (version 0.11.5)