##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840993.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193494 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.29761356152608 39.0 37.0 39.0 34.0 39.0 2 37.80894583466695 39.0 38.0 39.0 35.0 39.0 3 38.03878192936035 39.0 38.0 39.0 35.0 39.0 4 38.56966771512886 39.0 38.0 40.0 35.0 41.0 5 39.26608261122385 40.0 39.0 41.0 35.0 41.0 6 39.24525636492517 40.0 39.0 41.0 35.0 41.0 7 39.27843793098248 40.0 39.0 41.0 35.0 41.0 8 39.25089527052503 40.0 39.0 41.0 35.0 41.0 9 39.24078713424617 40.0 39.0 41.0 35.0 41.0 10 39.2049210134278 40.0 39.0 41.0 35.0 41.0 11 39.16123583361123 40.0 39.0 41.0 35.0 41.0 12 39.08274444613882 40.0 38.0 41.0 35.0 41.0 13 38.986689501581075 40.0 38.0 41.0 35.0 41.0 14 38.915668616683455 40.0 38.0 41.0 35.0 41.0 15 38.91921450799082 40.0 38.0 41.0 35.0 41.0 16 38.879154817703316 40.0 38.0 41.0 35.0 41.0 17 38.8434512448324 40.0 38.0 41.0 35.0 41.0 18 38.772857676703865 40.0 38.0 41.0 35.0 41.0 19 38.7793235659333 40.0 38.0 41.0 35.0 41.0 20 38.684249774192416 40.0 38.0 41.0 35.0 41.0 21 38.625823003718494 40.0 38.0 41.0 35.0 41.0 22 38.53160216976374 40.0 38.0 41.0 35.0 41.0 23 38.451858995520716 40.0 37.0 41.0 35.0 41.0 24 38.29683014744942 40.0 37.0 41.0 34.0 41.0 25 38.21006498591326 40.0 37.0 41.0 34.0 41.0 26 38.04959107435189 40.0 37.0 41.0 34.0 41.0 27 37.96712836669285 39.0 36.0 41.0 33.0 41.0 28 37.867337189396515 39.0 36.0 41.0 33.0 41.0 29 37.80552355555248 39.0 36.0 41.0 33.0 41.0 30 37.77992050461231 39.0 36.0 41.0 33.0 41.0 31 37.73619026109427 39.0 36.0 41.0 33.0 41.0 32 37.63150059092272 39.0 36.0 41.0 33.0 41.0 33 37.512196226978276 39.0 35.0 41.0 33.0 41.0 34 37.465984440379955 39.0 35.0 41.0 33.0 41.0 35 37.35225172644201 38.0 35.0 40.0 33.0 41.0 36 37.33997677195327 38.0 35.0 41.0 32.0 41.0 37 37.27169588034756 38.0 35.0 40.0 32.0 41.0 38 37.08843284674739 38.0 35.0 40.0 32.0 41.0 39 37.06862267654399 38.0 35.0 40.0 32.0 41.0 40 36.79139949851141 38.0 35.0 40.0 31.0 41.0 41 36.70965865229676 38.0 35.0 40.0 31.0 41.0 42 36.6024669929675 38.0 34.0 40.0 31.0 41.0 43 36.48104613464073 38.0 34.0 40.0 31.0 41.0 44 36.238646428036134 37.0 34.0 40.0 30.0 41.0 45 36.016584791127734 37.0 34.0 40.0 29.0 41.0 46 35.64963754935858 37.0 33.0 40.0 29.0 41.0 47 35.754684886959794 37.0 34.0 40.0 29.0 41.0 48 36.61760818911962 38.0 35.0 40.0 31.0 41.0 49 37.32305960171698 38.0 35.0 40.0 33.0 41.0 50 36.95881768591296 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 3.0 21 14.0 22 19.0 23 66.0 24 124.0 25 225.0 26 397.0 27 736.0 28 1538.0 29 3212.0 30 6821.0 31 12918.0 32 23999.0 33 41768.0 34 64351.0 35 67068.0 36 112383.0 37 193672.0 38 198114.0 39 189499.0 40 276566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.25955890854918 16.7529120380999 35.98752905335092 2 24.524463466091994 21.615860657866733 33.21658927485182 20.64308660118945 3 21.978158247967734 22.47602417775037 26.562764454618122 28.983053119663776 4 19.52519241822749 24.67888401617436 29.96554653814766 25.830377027450496 5 17.588777153467046 28.2394381538575 28.500101382998157 25.671683309677302 6 19.952341612106974 28.901862933538 27.589749089647707 23.556046364707324 7 24.05977742661463 25.698243979441877 21.964500868877433 28.27747772506607 8 22.585785936083465 23.531245234580147 30.085279021092692 23.797689808243693 9 22.933169333067447 27.481914446155574 27.0675009677468 22.51741525303018 10 28.488203543545254 21.486325025513324 26.622505014687963 23.402966416253452 11 24.74817636284724 22.89010250575202 24.79585150826062 27.565869623140127 12 22.5904780417832 26.398373179923823 25.39200029493236 25.619148483360622 13 23.401709602226738 23.97004090510719 25.890201375122118 26.73804811754395 14 20.069309104193234 25.73837824069497 26.820662692900004 27.37164996221179 15 20.913469192136702 30.93999634686056 25.31106147161192 22.835472989390816 16 20.691850985425987 32.73279966216839 22.09487437724865 24.480474975156977 17 21.406894379024948 33.83938251888991 21.208569125609344 23.545153976475795 18 20.378401567163305 27.474708712402407 23.298064338823657 28.84882538161063 19 22.72277866499538 23.741971053059338 24.584287813763623 28.950962468181658 20 21.844181872719933 23.13995713426293 27.734031339914573 27.28182965310257 21 21.942045791600126 30.21163072457842 24.296393614044142 23.54992986977731 22 24.344990423077117 26.878895076137795 21.893113832160026 26.883000668625062 23 26.298079420591975 25.81965221442253 26.030880758512403 21.851387606473093 24 24.845621343718527 26.81831664005014 23.054577568048103 25.281484448183235 25 22.56050223889017 27.616571323681715 23.25684002418237 26.566086413245742 26 22.493154931032702 31.096981055694684 25.774892295496947 20.63497171777567 27 23.67048964966118 29.516995547710046 22.62642509912437 24.18608970350441 28 23.757586049559333 26.157762385544565 22.85369259217329 27.230958972722814 29 22.63343425568185 30.55641432625265 24.558500707262436 22.251650710803066 30 25.78863057764968 27.994006120779797 24.801239801235457 21.41612350033507 31 24.65899123382933 29.09179880701644 22.849907120959852 23.399302838194384 32 20.41329311530931 28.498615300488613 26.25063943130303 24.83745215289905 33 20.222515181988424 24.93968068964782 29.962973360007677 24.874830768356084 34 23.0054352342208 29.12197244587327 24.35642555837806 23.51616676152787 35 19.507198492044292 26.235533544550254 25.197914690067797 29.059353273337656 36 20.599728975947293 26.549313432889864 26.55139544799081 26.299562143172032 37 20.0279468191149 32.658361515318404 22.61316413521891 24.700527530347788 38 23.177041391528427 27.23520842179241 23.87324484985139 25.71450533682777 39 21.97560618600168 29.938071859473663 25.789521651834413 22.296800302690244 40 17.797367788316485 28.483846441631165 27.97298162910453 25.745804140947815 41 20.864217886934426 30.73440907762297 24.63753588104357 23.763837154399035 42 18.973767188162512 30.557415380082553 23.374201206677235 27.094616225077694 43 18.906240903394888 25.932900100307222 25.371046048744915 29.789812947552974 44 21.175718315013324 26.36368414188583 23.17927266286057 29.281324880240277 45 20.09241093153702 26.24890505340266 30.664959147606485 22.99372486745383 46 19.330433116180433 28.8654612137076 26.90179004829998 24.90231562181199 47 21.033635155817336 30.2646834956118 25.60885115577369 23.09283019279718 48 22.103504302517575 27.750332040326008 24.764276086175588 25.381887570980826 49 18.618166112215164 28.461682184029367 22.88004192963683 30.04010977411864 50 16.672040293487164 32.329336516844144 28.015911744714966 22.98271144495373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18425.0 1 17056.0 2 16265.0 3 15960.5 4 13907.5 5 9851.5 6 5549.0 7 4240.5 8 4200.5 9 3926.5 10 3706.5 11 3402.5 12 3086.5 13 2964.5 14 3203.0 15 3493.5 16 3411.5 17 3360.0 18 3603.0 19 3805.5 20 3775.0 21 4101.0 22 4703.5 23 5242.0 24 5874.5 25 6945.5 26 8255.5 27 9948.5 28 12384.5 29 14516.0 30 16627.0 31 22405.0 32 30984.5 33 34911.0 34 39333.0 35 47239.0 36 48444.0 37 45621.5 38 45881.5 39 49268.5 40 54175.0 41 59592.0 42 64104.0 43 65192.5 44 65757.0 45 68979.5 46 73185.0 47 75953.0 48 84241.0 49 102841.5 50 124372.0 51 134515.5 52 125543.0 53 108329.0 54 99393.5 55 95425.0 56 87003.0 57 79859.5 58 74630.5 59 67368.0 60 59303.5 61 51780.0 62 43775.0 63 34922.0 64 27819.0 65 24088.5 66 18696.5 67 12384.0 68 8683.0 69 6637.0 70 5615.5 71 4480.0 72 3364.0 73 2252.5 74 1252.0 75 858.0 76 681.5 77 491.0 78 339.5 79 249.5 80 194.5 81 126.0 82 84.0 83 74.5 84 71.5 85 57.5 86 37.5 87 21.0 88 11.5 89 7.0 90 4.5 91 3.5 92 3.0 93 2.0 94 1.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 5852.0 25 6865.0 26 6999.0 27 7822.0 28 7970.0 29 7479.0 30 8161.0 31 8526.0 32 8144.0 33 6497.0 34 7210.0 35 7270.0 36 6184.0 37 5731.0 38 6525.0 39 9066.0 40 9777.0 41 8475.0 42 11160.0 43 20307.0 44 51399.0 45 104646.0 46 138327.0 47 126248.0 48 194735.0 49 182603.0 50 229516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.72736885759694 #Duplication Level Percentage of deduplicated Percentage of total 1 70.09915778828072 9.622769955666186 2 11.215736840334468 3.079251132340201 3 4.2337588297341995 1.7435510732960795 4 2.1053694193139494 1.1560473040170904 5 1.3282932989498863 0.9116986032879684 6 0.955085771587591 0.7866488806335263 7 0.6968772299038176 0.6696403548345061 8 0.515707182758 0.5663442176184976 9 0.44584801322803075 0.5508288118807143 >10 4.726718718937478 15.75647757918199 >50 2.202287787726699 21.75798240165952 >100 1.414987321766306 31.17403984250749 >500 0.03990340253862009 3.856117931425053 >1k 0.017101458230837183 4.060705202961539 >5k 0.001266774683765717 1.076756808630617 >10k+ 0.0019001620256485757 3.231139900059026 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 16203 1.357610511657369 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 10867 0.9105198685540103 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 10092 0.8455844771737436 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 6279 0.5261023515828316 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 6105 0.5115233088729395 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 4550 0.38123358810350116 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 4164 0.3488915738160393 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 3222 0.2699636529383474 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 2754 0.23075105530484444 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 2717 0.22765091403894783 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 2017 0.16899959279225535 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 1896 0.15886129297675564 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA 1703 0.14269028583302473 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 1622 0.13590349008876457 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 1585 0.13280334882286798 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 1512 0.12668685389285575 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 1469 0.12308398701627322 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1380 0.1156268904577652 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 1363 0.11420250122748836 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 1319 0.11051584674912483 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 1251 0.10481828982801757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05010498586503158 0.0 0.0 0.0 0.0 2 0.10431556421733164 0.0 0.0 0.0 0.0 3 0.13556833968164061 0.0 0.0 0.0 0.0 4 0.16305067306580512 0.0 0.0 0.0 0.0 5 0.18584090075023418 0.0 0.0 0.0 0.0 6 0.2310024181101874 0.0 0.0 0.0 0.0 7 0.2568089994587321 0.0 0.0 0.0 0.0 8 0.2863860228874213 0.0 0.0 0.0 0.0 9 0.3178063735552923 0.0 0.0 0.0 0.0 10 0.3447859813287708 0.0 0.0 0.0 0.0 11 0.3690005982434767 0.0 0.0 0.0 0.0 12 0.39656671922942216 0.0 0.0 0.0 0.0 13 0.4258923798527684 0.0 0.0 0.0 0.0 14 0.4531233504315899 0.0 0.0 0.0 0.0 15 0.48722490435645255 0.0 0.0 0.0 0.0 16 0.5141207245281502 0.0 0.0 0.0 0.0 17 0.5515737825242524 0.0 0.0 0.0 0.0 18 0.593383795812966 0.0 0.0 0.0 0.0 19 0.6206147663917875 0.0 0.0 0.0 0.0 20 0.6465051353421132 0.0 0.0 0.0 0.0 21 0.671473840672848 0.0 0.0 0.0 0.0 22 0.694264068357277 0.0 0.0 0.0 0.0 23 0.7212436761307556 0.0 0.0 0.0 0.0 24 0.7455420806472425 0.0 0.0 0.0 0.0 25 0.7732757768367499 0.0 0.0 0.0 0.0 26 0.804193401893935 0.0 0.0 0.0 0.0 27 0.8287431692157649 0.0 0.0 0.0 0.0 28 0.8590742810604829 0.0 0.0 0.0 0.0 29 0.8911649325426018 0.0 0.0 0.0 0.0 30 0.9212446815819769 0.0 0.0 0.0 0.0 31 0.9504027670017612 0.0 0.0 0.0 0.0 32 0.9789743392090785 0.0 0.0 0.0 0.0 33 1.006708035398586 0.0 0.0 0.0 0.0 34 1.038044598464676 0.0 0.0 0.0 0.0 35 1.0644376930256876 0.0 0.0 0.0 0.0 36 1.0895739735599843 0.0 0.0 0.0 0.0 37 1.10783967074824 0.0 0.0 0.0 0.0 38 1.1207429614225124 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGAAC 40 0.0 205.69316 44 ATTCGTC 10 0.0024903105 205.69316 44 GACCGCA 30 5.638867E-11 205.69315 44 GACCCGT 15 3.0492278E-5 205.69315 44 CTATACG 50 1.2732926E-11 143.98521 44 CTATCAT 1715 0.0 140.3271 44 CGGCAAA 30 2.8081213E-6 137.12877 44 CCGGGCT 75 0.0 123.415886 44 TCAACGA 35 6.04496E-6 117.53895 44 ACGGGTC 25 1.4706893E-7 115.09969 43 ACCGTCA 20 6.7155015E-6 115.099686 43 TGCGTCA 45 1.5330443E-7 114.27398 44 AATCGCA 210 0.0 112.64149 44 ACCGCAC 55 3.8635335E-9 112.196266 44 TCGTGCG 120 0.0 110.30386 43 AAAGGCG 45 5.456968E-12 102.31084 43 GTGCGTC 40 2.0736479E-10 100.71223 43 GATTTTC 345 0.0 95.39393 44 GCGCGAG 25 2.0346466E-5 92.07975 43 TGAACGT 25 2.0346466E-5 92.07975 43 >>END_MODULE