FastQCFastQC Report
Tue 24 May 2016
ERR840992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52115
Sequences flagged as poor quality0
Sequence length24-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT15963.0624580255204834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA7241.3892353449102945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA7121.3662093447184114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA7081.3585340113211168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA6331.2146215101218458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5341.0246570085388085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.9536601746138348No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA4950.9498225079151875No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC4850.9306341744219515No Hit
AAAAAAAAAAAAAAAAAAAAAAAA4060.7790463398253862No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA3310.6351338386261153No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG3080.5910006715916722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.5910006715916722No Hit
CTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGCAC3060.5871630048930251No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC2750.5276791710639931No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA2380.4566823371390195No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT2210.42406217020051806No Hit
TCAGAGAGATGAGATGGGAGGAAGAGGACAGATTTGAAGTGTGAGAG2130.40871150340592916No Hit
AGCCTTTAAATGGGTGCTTCCACCACTACAGGCTCCTGACACGAGTAACA2070.3971985033099875No Hit
CTCATTCTCTCTTGCGCCGCCACATAAGAAGTACCGTTCGCCTCCCGCCA1980.37992900316607503No Hit
ACTTCCTATCAAAGATAAAAGTGGTATAATCCCACAGACTAGGGAGAAGC1840.35306533627554443No Hit
CATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG1830.35114650292622085No Hit
CAACCCTCTTGGGTTGCCTTCCATGCTGTGGAAGG1770.3396335028302792No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA1760.3377146694809556No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGG1710.3281205027343375No Hit
TTAAGACCTGCAATAATCCAGAATGGCTACTCTGATCTATGTTGATAA1630.31276983593974866No Hit
AGATGCAGATTTGAATCCTGGCTCCAGCATGTACTAAGTCAACTTTTGA1590.3050945025424542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.30317566919313055No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATG1550.29741916914515976No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG1540.2955003357958361No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT1520.2916626690971889No Hit
CCCAGCGGTTCAGCTGAGGTAGGGACGTGCTGTAGGCCGGAATGTTACC1500.2878250023985417No Hit
CAACTCGCTGGGGTGTGCTTCCAGTTGGTGGACGCTTTGTGCTCTGC1490.28590616904921806No Hit
CTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGC1470.28206850235057085No Hit
CCGAGAGAACTTTGAGCGTTAGCTGTCTCTTGGACTCTTAATTCGTCTCA1470.28206850235057085No Hit
TTCTTTTCGCTCTTTGCAATAAATCTTTCTGCTGTTTACACTTTGGGTC1440.27631200230260006No Hit
CTCCCGCTGTGCAGCTCCCCTTCAAGACCCTGCCCTATCAGCTCAAGTC1430.2743931689532764No Hit
CCTTACCCTGCCATTTCTAGTTACGTCAAGGATCTTACTGCTCCAGGAC1380.26479900220665836No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACAC1360.26096133550801115No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGGCT1360.26096133550801115No Hit
TGGCAGCCATCTCCTTCTCGGCATCATGGCCGCCCTCAGACCCCTTGTG1350.2590425021586875No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC1310.2513671687613931No Hit
AGCCTTTAAATGGGTGCTTCCACCACTACAGGCTCCTGACACGAGTAA1300.24944833541206946No Hit
TTCTTATCCTCAGGTCCGTGGTTTTCAGGGAGTTGGTAGTGAATCCTTTA1280.24561066871342224No Hit
AGTGTAACAGCTGCTTGTTATGATCCTAGCAAGCCACAGACACAAATGCA1270.24369183536409864No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA1260.241773002014775No Hit
CCTTCTTTCTATCACTCCCCCCATCCCTCCCTCCAACTCTCAGGTACTTT1220.23409766861748055No Hit
CTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAG1190.22834116856950976No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA1190.22834116856950976No Hit
AAGCCCTGCAGCAGAAACAGTGATGGCTCCTCCTCCTCTTCCCCTCCCT1180.22642233522018612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.22642233522018612No Hit
CATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA1160.22258466852153888No Hit
CAGGAGGCTGTTGTGGATCTTCATGCTGATGACTCTCGCATCTCT1150.2206658351722153No Hit
TCAGAGAGATGAGATGGGAGGAAGAGGACAGATTTGAAGTGTGAG1150.2206658351722153No Hit
AAGGTTTCCGTAGGAACCTGCGGAAGGATCATTATTTGTTTGTCCAGCAC1140.2187470018228917No Hit
TCCTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCG1130.21682816847356806No Hit
TTTGGAAAGGAGAATTCAACAATTAATAGTTGAAATTGTGAGGTTAATGT1110.21299050177492085No Hit
TGAAGCAGTGCTTCGTGGTGACTGAAAAAGGGAGTAAGGCATGTCGAG1100.21107166842559724No Hit
ACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCTCCTG1080.20723400172695003No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAA1080.20723400172695003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.20723400172695003No Hit
ACTTCCTATCAAAGATAAAAGTGGTATAATCCCACAGACTAGGGAGAA1060.2033963350283028No Hit
CAACTCGCTGGGGTGTGCTTCCAGTTGGTGGACGCTTTGTGCTCT1040.19955866832965558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.19955866832965558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.19188333493236112No Hit
CATTGAGCCTTTATCAAGACCCACTCTATCCTGGGTTTCCTTGTAATGAA1000.19188333493236112No Hit
ATGAAGGACAGTATGATGGGAAAGTTGATATTTGGTCACTTGGCATCACT990.18996450158303751No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTT990.18996450158303751No Hit
ACTCCAGAGGAGTGTGAGGAGACGAGTGAAAAACCCAAAAAGAAGAAAAA990.18996450158303751No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA980.1880456682337139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.1880456682337139No Hit
AACTGCAATGGTTTCAAAATCATCTTGATCCCCCAAAAAAGAGATATTCA980.1880456682337139No Hit
ACTTCCCTCCATGTGATGCTGTCCACCATGTTATGATGCAGGATGAGGCC980.1880456682337139No Hit
CTCAACATTGAAGCTGCTAAGGATCTCTTATACAGACGCACCAAA960.18420800153506667No Hit
CCTTACCCTGCCATTTCTAGTTACGTCAAGGATCTTACTGCTCCAGG960.18420800153506667No Hit
CCGAGAGAACTTTGAGCGTTAGCTGTCTCTTGGACTCTTAATTCGTCT960.18420800153506667No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC960.18420800153506667No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAATGG950.18228916818574306No Hit
TGGCAGCCATCTCCTTCTCGGCATCATGGCCGCCCTCAGACCCCTTG940.18037033483641945No Hit
ACCGGGACCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGATC940.18037033483641945No Hit
AGATGCAGATTTGAATCCTGGCTCCAGCATGTACTAAGTCAACTTTT930.17845150148709585No Hit
AGATGCGGCGCAGGAGCTGTCGCTGTGTTTGCTTTAACCTGAGTCTTGTT920.17653266813777221No Hit
CTCATTCTCTCTTGCGCCGCCACATAAGAAGTACCGTTCGCCTCCCGC910.1746138347884486No Hit
AGTGTAACAGCTGCTTGTTATGATCCTAGCAAGCCACAGACACAAATG910.1746138347884486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.17269500143912503No Hit
CGGGATAGTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAA880.1688573347404778No Hit
TGAAGCAGTGCTTCGTGGTGACTGAAAAAGGGAGTAAGGCATGTCGAGGC880.1688573347404778No Hit
AGCCTTTAAATGGGTGCTTCCACCACTACAGGCTCCTGACACGAGT870.16693850139115418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.16693850139115418No Hit
CTTTTGCGTTCTCTTTCCCTCTCCCAACATGGCGG860.16501966804183058No Hit
CTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACG850.16310083469250697No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA840.16118200134318333No Hit
TGAAGCAGTGCTTCGTGGTGACTGAAAAAGGGAGTAAGGCATGTCG810.15542550129521251No Hit
TCCTTGAAGTCAGAGACTGGAAATGTTGATCTCTTAGTCTTCTATAGACA810.15542550129521251No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATG810.15542550129521251No Hit
CTCATTCTCTCTTGCGCCGCCACATAAGAAGTACCGTTCGCCTCCC810.15542550129521251No Hit
TTCTTTTCGCTCTTTGCAATAAATCTTTCTGCTGTTTACACTTTGGG790.15158783459656527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.14775016789791806No Hit
CTCCCGCTGTGCAGCTCCCCTTCAAGACCCTGCCCTATCAGCTCAAG770.14775016789791806No Hit
CCATTTTGCGGGAAGAGGAGGCGCTGTACCTGCAGTGCTGCTTTTCTTGC760.14583133454859445No Hit
TCAGAGAGATGAGATGGGAGGAAGAGGACAGATTTGAAGTGTG750.14391250119927085No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT750.14391250119927085No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC750.14391250119927085No Hit
AACTGCAATGGTTTCAAAATCATCTTGATCCCCCAAAAAAGAGATATT750.14391250119927085No Hit
AAGGTTTCCGTAGGAACCTGCGGAAGGATCATTATTTGTTTGTCCAGC740.1419936678499472No Hit
ACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCTCC740.1419936678499472No Hit
CTGCCGCTATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGC730.14007483450062364No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAA730.14007483450062364No Hit
CATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATT720.13815600115130003No Hit
ATGAAGGACAGTATGATGGGAAAGTTGATATTTGGTCACTTGGCATCA720.13815600115130003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.13815600115130003No Hit
TTAAGACCTGCAATAATCCAGAATGGCTACTCTGATCTATGTTGAT720.13815600115130003No Hit
TTGTGTTGATTCAAATTATGTGTTCTTTGGAAAGTCATGTCAGTGGTAGT710.1362371678019764No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1362371678019764No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC710.1362371678019764No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGG700.1343183344526528No Hit
CAACCCGCTGGGGTCCCCTTCCACACTGTGGAAGG700.1343183344526528No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1343183344526528No Hit
ACTGAAGAAGAAAAGCATTCCTCAGCCCACAAATGCCCTGAAATCC680.13048066775400557No Hit
CACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGC670.12856183440468197No Hit
ACTGCAGACTGAGCGGTGGACCGAATTGGGACCGCTGGCTTATAA670.12856183440468197No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA660.12664300105535833No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGAT660.12664300105535833No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACA660.12664300105535833No Hit
ACTTCCTATCAAAGATAAAAGTGGTATAATCCCACAGACTAGGGAG660.12664300105535833No Hit
AGTGTAACAGCTGCTTGTTATGATCCTAGCAAGCCACAGACACAAA650.12472416770603473No Hit
ACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCT650.12472416770603473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.12472416770603473No Hit
CCAGGTCTTAGTCATCTCCGCAGTCTCAACTCCCAGCAGAGTCT650.12472416770603473No Hit
TCCTTGAAGTCAGAGACTGGAAATGTTGATCTCTTAGTCTTCTATAGA640.12280533435671112No Hit
TTAAGACCTGCAATAATCCAGAATGGCTACTCTGATCTATGTTG640.12280533435671112No Hit
CCTTCTTTCTATCACTCCCCCCATCCCTCCCTCCAACTCTCAGGTACT630.1208865010073875No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC630.1208865010073875No Hit
AGATGCAGATTTGAATCCTGGCTCCAGCATGTACTAAGTCAACTT630.1208865010073875No Hit
ACTTCCCTCCATGTGATGCTGTCCACCATGTTATGATGCAGGATGAGG620.1189676676580639No Hit
TTCTTTTCGCTCTTTGCAATAAATCTTTCTGCTGTTTACACTTTG620.1189676676580639No Hit
CATTGAGCCTTTATCAAGACCCACTCTATCCTGGGTTTCCTTGTAATG620.1189676676580639No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC620.1189676676580639No Hit
CGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT610.11704883430874027No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.11704883430874027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11513000095941667No Hit
ACCGGGACCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGA600.11513000095941667No Hit
ACTCCAGAGGAGTGTGAGGAGACGAGTGAAAAACCCAAAAAGAAGAAA600.11513000095941667No Hit
TCTAGGGAGGAAGAAGTTGACACACTTGGTAACGGCTG590.11321116761009306No Hit
CAACCCTCTTGGGTTGCCTTCCATGCTGTGGAAGGTTTGTTCT590.11321116761009306No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC590.11321116761009306No Hit
CCCAGCGGTTCAGCTGAGGTAGGGACGTGCTGTAGGCCGGAATGT590.11321116761009306No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGCCGCGAAACCTG580.11129233426076944No Hit
ACCGCGGCCTCAGATGAATGCGGCTG580.11129233426076944No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACG580.11129233426076944No Hit
TCCTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCT580.11129233426076944No Hit
CAACTCGCTGGGGTGTGCTTCCAGTTGGTGGACGCTTTGTGCT570.10937350091144585No Hit
CATTCCGTATGGAGAGCCGGGAATAAGTTCCCCTGTCAGTGGAGGA570.10937350091144585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10937350091144585No Hit
TTTATGTGCTTACCTCCTCAAAGCAATACACTGAATGTTTAGACG570.10937350091144585No Hit
CATTAAAACGAGTCTTCACTACCCAGAATCATTTAATCATCCATTTCA560.10745466756212224No Hit
CAACAGTACATAGTACATAAAGCCATTCATCGTACATAGCACATTA560.10745466756212224No Hit
CTCTCTGATTTGGTGTCTATTTGTAAATCTGTTCAATACAGGGATTTTGA560.10745466756212224No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10745466756212224No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT560.10745466756212224No Hit
AGATGCGGCGCAGGAGCTGTCGCTGTGTTTGCTTTAACCTGAGTCTTG560.10745466756212224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10553583421279862No Hit
CTTTTTGTGTTTCTCTGCGTCCAGAGCTCCAGTTCTTCTCTTCACT550.10553583421279862No Hit
CTCCCGCTGTGCAGCTCCCCTTCAAGACCCTGCCCTATCAGCTCA550.10553583421279862No Hit
TGGCAGCCATCTCCTTCTCGGCATCATGGCCGCCCTCAGACCCCT550.10553583421279862No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10553583421279862No Hit
CATTAAAACGAGTCTTCACTACCCAGAATCATTTAATCATCCATTTCATC550.10553583421279862No Hit
TTTGTCTCTCCCTTCAGCCCGAGCTCCGGGTCTCGTCTTCACTGC540.10361700086347501No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA540.10361700086347501No Hit
CCGAGAGAACTTTGAGCGTTAGCTGTCTCTTGGACTCTTAATTCGT540.10361700086347501No Hit
AAGCCTTTGCTGCCTAGTCAGAAAGACACATGTCAGGACTGCTTTG530.1016981675141514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAG105.353384E-4340.6238444
CATCACT202.7523129E-8340.6238444
ATAGACA153.859046E-6340.6238444
TTAATGT105.353384E-4340.6238444
TGGTGAG105.353384E-4340.6238444
TCGAGGC105.353384E-4340.6238444
CGTCTCA153.859046E-6340.6238444
CTTAGCC700.0267.6330344
GAGAAGC401.0913936E-11255.4678844
AGTAACA201.2168937E-5255.4678844
TGAGGCC201.2168937E-5255.4678844
CAAGATC302.0803418E-7227.0825744
TCCTTTA302.0803418E-7227.0825744
CCTCCTG150.001802904227.0825744
CCCGCCA252.9642206E-5204.374344
ATTTTGA252.9642206E-5204.374344
TCAAGTC100.0025966847201.7826143
GTCGAGG100.0025966847201.7826143
AGGAACA100.0025966847201.7826143
GAGTAAC153.1904674E-5201.7826143
Produced by FastQC (version 0.11.5)