FastQCFastQC Report
Tue 24 May 2016
ERR840991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921048
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC31660.34373887137261033No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT24500.26600133760672623No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA20990.2278925745455177No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA20780.2256125630803172No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG16690.18120662549617392No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC13860.15048075670323371No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA13060.1417949987405651No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA12630.1371264038356307No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT12220.13267495287976305No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG11600.12594349045869488No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG11100.120514891732027No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA10260.11139484587122496No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC10050.10911483440602444No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC9890.10737768281349072No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT9620.10444623950109007No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG9520.10336051975575648No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG9510.10325194778122314No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9320.10118908026508933No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA9280.10075479236695589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTG550.0220.0464644
GTCGTAT100.002034547220.0464544
AATAGCC258.091065E-7176.0371744
CTCGAAA600.0165.0348544
ACTGGCG207.333369E-5165.0348444
TAATAGG600.0146.6976544
TCCGAAC150.0068432656146.6976544
GGGGTAC150.0068432656146.6976544
TAACTCG150.0068432656146.6976544
CTAGTAC551.4551915E-11140.0295744
CGATACG251.7838881E-4132.0278844
CTATCAT3700.0124.8912344
CGAACTC152.5820936E-4120.3401743
CGATCAT251.12700945E-7120.34016443
TGGTAAA751.8189894E-12117.35811644
TCGTGCG1150.0115.10799443
GACCGCC801.8189894E-12110.02322444
GGGAATA408.388455E-6110.02322444
CTGGTAA500.0108.30614543
ACTTCTA2000.0104.5220744
Produced by FastQC (version 0.11.5)