##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840991.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 921048 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.35050181966629 39.0 37.0 39.0 34.0 39.0 2 37.869555115477155 39.0 38.0 39.0 35.0 39.0 3 38.07556283711598 39.0 38.0 39.0 35.0 39.0 4 38.60198599855816 39.0 38.0 40.0 35.0 41.0 5 39.26451064439638 40.0 39.0 41.0 35.0 41.0 6 39.22354318124571 40.0 38.0 41.0 35.0 41.0 7 39.28165958777393 40.0 39.0 41.0 36.0 41.0 8 39.222171917207355 40.0 39.0 41.0 35.0 41.0 9 39.1968822471793 40.0 39.0 41.0 35.0 41.0 10 39.13694726007765 40.0 39.0 41.0 35.0 41.0 11 39.09161737499023 40.0 38.0 41.0 35.0 41.0 12 39.029825807124055 40.0 38.0 41.0 35.0 41.0 13 38.94199759404504 40.0 38.0 41.0 35.0 41.0 14 38.89422809668986 40.0 38.0 41.0 35.0 41.0 15 38.87477199885348 40.0 38.0 41.0 35.0 41.0 16 38.817673997446384 40.0 38.0 41.0 35.0 41.0 17 38.75364693262458 40.0 38.0 41.0 35.0 41.0 18 38.76043268103291 40.0 38.0 41.0 35.0 41.0 19 38.70270930505251 40.0 38.0 41.0 35.0 41.0 20 38.630734771694854 40.0 38.0 41.0 35.0 41.0 21 38.56369917745872 40.0 38.0 41.0 35.0 41.0 22 38.43016867741964 40.0 38.0 41.0 34.0 41.0 23 38.356189905412094 40.0 37.0 41.0 34.0 41.0 24 38.203106678479294 40.0 37.0 41.0 34.0 41.0 25 38.085827450381345 40.0 37.0 41.0 34.0 41.0 26 37.946922077138645 39.0 36.0 41.0 33.0 41.0 27 37.87916975782582 39.0 36.0 41.0 33.0 41.0 28 37.73989072613779 39.0 36.0 41.0 33.0 41.0 29 37.62845289846585 39.0 36.0 41.0 33.0 41.0 30 37.585258628333236 39.0 36.0 41.0 33.0 41.0 31 37.49355639203196 39.0 35.0 41.0 33.0 41.0 32 37.360562087663084 39.0 35.0 40.0 32.0 41.0 33 37.318451091876184 38.0 35.0 40.0 32.0 41.0 34 37.236851242806004 38.0 35.0 40.0 32.0 41.0 35 37.172514897970075 38.0 35.0 40.0 32.0 41.0 36 37.0704862208835 38.0 35.0 40.0 32.0 41.0 37 37.008556405746354 38.0 35.0 40.0 32.0 41.0 38 36.88214491653037 38.0 35.0 40.0 32.0 41.0 39 36.71757187815704 38.0 35.0 40.0 31.0 41.0 40 36.56819211707807 38.0 34.0 40.0 31.0 41.0 41 36.41463564649938 37.0 34.0 40.0 31.0 41.0 42 36.25361217562152 37.0 34.0 40.0 31.0 41.0 43 36.09126239630531 37.0 34.0 40.0 30.0 41.0 44 35.88286123910129 37.0 34.0 40.0 30.0 41.0 45 35.67087087087087 37.0 34.0 40.0 29.0 41.0 46 35.36124600772238 36.0 33.0 40.0 28.0 41.0 47 35.45104971983829 36.0 33.0 39.0 29.0 41.0 48 36.235412157919605 37.0 34.0 40.0 31.0 41.0 49 36.84716289922516 38.0 35.0 40.0 32.0 41.0 50 36.89310542878681 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 7.0 22 10.0 23 23.0 24 63.0 25 125.0 26 239.0 27 549.0 28 1198.0 29 2432.0 30 5261.0 31 10119.0 32 18776.0 33 32792.0 34 53877.0 35 57858.0 36 94868.0 37 153535.0 38 152801.0 39 152492.0 40 184021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.41370699464088 20.522274626295264 32.064018379063846 2 26.37549834536311 21.204540914262886 29.27578150107269 23.14417923930132 3 23.141464939937983 20.83963050785627 27.964340620684265 28.054563931521486 4 19.91307727718859 22.971984087691414 33.62083192189766 23.49410671322233 5 19.649790238945204 22.697188420147484 32.62316404791064 25.02985729299667 6 21.05601445310125 27.58488156969018 29.33137035203377 22.0277336251748 7 27.572070076695244 25.082297556696286 23.120510548853048 24.225121817755426 8 26.460184485499127 23.882034378230017 27.190873874108622 22.466907262162234 9 26.809460527572938 24.575266435625505 25.672060522361484 22.943212514440074 10 24.534877661099095 22.30806646341993 27.55610999643884 25.600945879042136 11 26.88622091356802 22.93376675265567 27.92319184233612 22.256820491440184 12 24.778296028002885 24.73161007895354 25.847187117283788 24.642906775759787 13 24.976765597449862 23.02203576795129 28.185718876757782 23.815479757841068 14 22.002653499057594 24.345310993563853 26.816517705917608 26.835517801460945 15 23.41984348264151 27.001307206573383 25.152326480270297 24.426522830514806 16 23.48151236417646 29.681514969903848 22.47743874369197 24.359533922227723 17 24.28157924451277 28.4214286334697 23.150910701722385 24.146081420295143 18 23.837302724722274 27.503995448662828 22.366478185718876 26.292223640896022 19 25.76467241663844 22.82324048258071 25.494002484126778 25.918084616654074 20 25.03843447898481 23.2069338405816 28.40470854939156 23.34992313104203 21 26.465613084225797 26.46181306511713 23.39226620111004 23.68030764954704 22 26.29211506892149 27.172633782387017 23.618964483935688 22.9162866647558 23 28.93334549339448 24.710655687868602 23.32690587244096 23.02909294629596 24 26.118291337693584 26.97470707281271 24.69350131589233 22.213500273601376 25 26.169079859027377 25.200493185959477 26.46040873331951 22.170018221693635 26 26.959258995212426 28.27389537530094 24.398865898534115 20.367979730952523 27 28.036161325988772 28.27669300903813 21.64178857683951 22.045357088133585 28 26.583689207126866 26.533232938295864 22.37120067068025 24.51187718389702 29 26.99872944268381 25.599108490423966 24.168253468933816 23.233908597958408 30 28.865900701355464 27.992713714759667 23.40578839918338 19.735597184701493 31 25.86468988793529 27.09595399781933 25.210375826234095 21.828980288011294 32 23.120472406874768 27.955674279741206 27.41310323310856 21.510750080275464 33 24.78773211965377 25.417030472615437 28.21002889822371 21.58520850950708 34 23.889422738614485 27.292935841517004 26.744871485772357 22.072769934096158 35 24.22123456561022 26.229242007056385 25.332232271473604 24.21729115585979 36 23.097562654353755 26.945192678980355 27.244188366116312 22.713056300549578 37 25.722020072116937 26.668950878111115 23.838132328593204 23.770896721178747 38 24.440086647571498 25.44712293035766 23.41677741314312 26.696013008927714 39 24.513778501667332 27.48482542561817 24.212127075856845 23.78926899685765 40 21.130726417293886 28.654355454855235 25.58225176806777 24.63266635978311 41 23.924933156934372 29.272016604336088 24.188807992464852 22.614242246264688 42 23.85616465004085 29.764521651169122 23.569962650274288 22.80935104851574 43 23.30191685233188 28.29070312471166 25.12010314414961 23.287276878806853 44 23.5668485683878 28.830522296258977 23.091633986763853 24.51099514858937 45 24.121820935980228 28.497302612346857 26.170373913736743 21.210502537936165 46 22.825102488321097 29.640426875774622 23.845963628563258 23.688507007341023 47 22.816866444428683 28.163167600539047 25.360131924249945 23.659834030782324 48 23.101039807876127 28.38293584435032 25.90975097691018 22.60627337086337 49 21.093532985401914 30.496504890538855 24.63504782695368 23.774914297105546 50 23.133069292714154 30.305242123652377 24.423557847630214 22.138130736003255 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7560.0 1 6557.5 2 7329.0 3 8834.0 4 7799.5 5 5473.5 6 3206.5 7 2577.5 8 2358.0 9 1880.5 10 1639.5 11 1590.5 12 1614.0 13 1686.0 14 1950.5 15 2232.0 16 2221.0 17 2106.5 18 2100.5 19 2015.0 20 2014.0 21 2747.5 22 3737.5 23 4392.5 24 4753.0 25 5866.5 26 7758.0 27 9106.5 28 10165.5 29 11180.5 30 12417.5 31 16207.5 32 21479.5 33 24419.0 34 29162.5 35 33428.0 36 33501.5 37 35012.0 38 35885.5 39 36944.0 40 41037.0 41 45444.5 42 49538.0 43 52574.5 44 55237.5 45 58628.0 46 63730.5 47 66823.0 48 69094.5 49 73863.0 50 80215.0 51 84765.0 52 85286.0 53 82396.5 54 76347.0 55 71756.5 56 69772.0 57 66213.5 58 61680.5 59 58785.0 60 55569.5 61 50591.0 62 44342.5 63 36953.0 64 28985.0 65 22285.0 66 16435.5 67 12372.0 68 9462.0 69 6862.5 70 4932.5 71 3175.5 72 2130.0 73 1750.5 74 1475.0 75 1250.5 76 1103.0 77 869.5 78 561.5 79 293.0 80 139.5 81 63.0 82 45.0 83 37.5 84 30.5 85 19.5 86 12.5 87 10.0 88 5.5 89 3.0 90 2.5 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 4558.0 25 4755.0 26 4578.0 27 5386.0 28 5314.0 29 5468.0 30 6857.0 31 5906.0 32 5508.0 33 5849.0 34 4671.0 35 4995.0 36 4976.0 37 4194.0 38 5670.0 39 5998.0 40 7184.0 41 6685.0 42 9653.0 43 16992.0 44 43271.0 45 81284.0 46 107336.0 47 100509.0 48 157452.0 49 138853.0 50 167146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.138441011730254 #Duplication Level Percentage of deduplicated Percentage of total 1 67.69020288923325 8.893437377322435 2 11.455406944810013 3.0101237681950286 3 4.454797604065643 1.7558728662064114 4 2.331716313470574 1.2254046896248907 5 1.4797327638702367 0.9720690815616838 6 0.9988076107009063 0.7873664925156655 7 0.7739478745015815 0.7117927946705125 8 0.6169206036718216 0.6484299968210596 9 0.5285427598858996 0.6249805085644198 >10 5.517091012635745 17.34455216458828 >50 2.4436508592445407 22.979631990681206 >100 1.6559853084065883 34.85154939950958 >500 0.04204315031705846 3.8342036511420403 >1k 0.011154305186158366 2.360585218596799 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 3166 0.34373887137261033 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 2450 0.26600133760672623 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 2099 0.2278925745455177 No Hit CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA 2078 0.2256125630803172 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 1669 0.18120662549617392 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 1386 0.15048075670323371 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 1306 0.1417949987405651 No Hit CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA 1263 0.1371264038356307 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 1222 0.13267495287976305 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 1160 0.12594349045869488 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 1110 0.120514891732027 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 1026 0.11139484587122496 No Hit CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC 1005 0.10911483440602444 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 989 0.10737768281349072 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 962 0.10444623950109007 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 952 0.10336051975575648 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG 951 0.10325194778122314 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 932 0.10118908026508933 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 928 0.10075479236695589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0333315961817408 0.0 0.0 0.0 0.0 2 0.06557747261814802 0.0 0.0 0.0 0.0 3 0.09054902676082029 0.0 0.0 0.0 0.0 4 0.11443486115815897 0.0 0.0 0.0 0.0 5 0.13061208536362925 0.0 0.0 0.0 0.0 6 0.16285796180003648 0.0 0.0 0.0 0.0 7 0.18261806116510756 0.0 0.0 0.0 0.0 8 0.20867533505311342 0.0 0.0 0.0 0.0 9 0.2340811770939191 0.0 0.0 0.0 0.0 10 0.2535155605353901 0.0 0.0 0.0 0.0 11 0.27142993633339413 0.0 0.0 0.0 0.0 12 0.2930357592655323 0.0 0.0 0.0 0.0 13 0.3144244382486038 0.0 0.0 0.0 0.0 14 0.3385274165950092 0.0 0.0 0.0 0.0 15 0.36111038729794753 0.0 0.0 0.0 0.0 16 0.3807619146884853 0.0 0.0 0.0 0.0 17 0.4048648930348907 0.0 0.0 0.0 0.0 18 0.43037930705022975 0.0 0.0 0.0 0.0 19 0.4536137096003683 0.0 0.0 0.0 0.0 20 0.4751109605579731 0.0 0.0 0.0 0.0 21 0.49910536692984514 0.0 0.0 0.0 0.0 22 0.5203854739383832 0.0 0.0 0.0 0.0 23 0.540362717252521 0.0 0.0 0.0 0.0 24 0.5653342713951933 0.0 0.0 0.0 0.0 25 0.590740113435999 0.0 0.0 0.0 0.0 26 0.6163630994258714 0.0 0.0 0.0 0.0 27 0.6381860663070763 0.0 0.0 0.0 0.0 28 0.6622890446534817 0.0 0.0 0.0 0.0 29 0.6867177389234872 0.0 0.0 0.0 0.0 30 0.712775012811493 0.0 0.0 0.0 0.0 31 0.7401351503938991 0.0 0.0 0.0 0.0 32 0.7669524281036385 0.0 0.0 0.0 0.0 33 0.7907296905264438 0.0 0.0 0.0 0.0 34 0.8161355325672495 0.0 0.0 0.0 0.0 35 0.842409950404322 0.0 0.0 0.0 0.0 36 0.8633643414892601 0.0 0.0 0.0 0.0 37 0.8799758535928638 0.0 0.0 0.0 0.0 38 0.8924616306641999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCTTG 55 0.0 220.04646 44 GTCGTAT 10 0.002034547 220.04645 44 AATAGCC 25 8.091065E-7 176.03717 44 CTCGAAA 60 0.0 165.03485 44 ACTGGCG 20 7.333369E-5 165.03484 44 TAATAGG 60 0.0 146.69765 44 TCCGAAC 15 0.0068432656 146.69765 44 GGGGTAC 15 0.0068432656 146.69765 44 TAACTCG 15 0.0068432656 146.69765 44 CTAGTAC 55 1.4551915E-11 140.02957 44 CGATACG 25 1.7838881E-4 132.02788 44 CTATCAT 370 0.0 124.89123 44 CGAACTC 15 2.5820936E-4 120.34017 43 CGATCAT 25 1.12700945E-7 120.340164 43 TGGTAAA 75 1.8189894E-12 117.358116 44 TCGTGCG 115 0.0 115.107994 43 GACCGCC 80 1.8189894E-12 110.023224 44 GGGAATA 40 8.388455E-6 110.023224 44 CTGGTAA 50 0.0 108.306145 43 ACTTCTA 200 0.0 104.52207 44 >>END_MODULE