FastQCFastQC Report
Tue 24 May 2016
ERR840989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639079
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC47790.7477948735602328No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA30090.4708338092786651No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT17970.2811858940756933No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG12020.1880831634273697No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC10640.16648958892406104No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT10630.1663331137465008No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10270.16070000735433335No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC8890.13910643285102467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA8810.13785463143054302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA8710.13628987965494094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA8490.13284742574861638No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG8110.12690136900132848No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT8020.1254930924032866No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACCC7960.12455424133792536No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.12298948956232328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7360.11516573068431289No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC7260.11360097890871082No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT7220.11297507819846997No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA7160.11203622713310873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA7160.11203622713310873No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.11187975195554854No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890.10781139733898314No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC6810.10655959591850148No Hit
TCTCATTGAACTCGCCTGCAGCTCTTGGGTTTTTTGTGGCTT6580.1029606668346167No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA6560.10264771647949626No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.10249124130193607No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT6430.10061353917121359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT100.0031918192189.2919544
CATATAC154.243203E-5189.2919544
CAACGGT205.631464E-7189.2919544
AACGGTG205.631464E-7189.2919544
GATATAC100.0031918192189.2919544
GCTAGTT205.631464E-7189.2919544
ACGAACA850.0167.0223144
ACCTGCC451.0913936E-11147.2270844
AGAATCG355.606853E-8135.2085644
AATCGCA900.0126.1946444
GGGCGTA302.5393092E-9119.1706343
GGCATCG152.683298E-4119.1706343
TAACTAA152.683298E-4119.1706343
CGCCAGT302.5393092E-9119.1706343
GTTCGGC500.0119.1706243
GAGTTAT251.193348E-7119.1706243
TAACGGT205.643409E-6119.17061643
AGACCGC205.643409E-6119.17061643
GACGATT205.643409E-6119.17061643
AGAGCCA1650.0114.722444
Produced by FastQC (version 0.11.5)