##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840989.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639079
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.156130932169575	39.0	35.0	39.0	30.0	39.0
2	37.04796120667398	39.0	37.0	39.0	33.0	39.0
3	37.294843047573146	39.0	37.0	39.0	33.0	39.0
4	37.74984156888272	39.0	37.0	39.0	34.0	41.0
5	38.54991636401759	40.0	37.0	41.0	35.0	41.0
6	38.520503724891604	40.0	37.0	41.0	35.0	41.0
7	38.48855775264091	40.0	37.0	41.0	35.0	41.0
8	38.48637648866572	40.0	37.0	41.0	35.0	41.0
9	38.486977353347555	40.0	37.0	41.0	35.0	41.0
10	38.3900159448206	40.0	37.0	41.0	34.0	41.0
11	38.28838844649879	40.0	36.0	41.0	34.0	41.0
12	38.12991821042469	40.0	36.0	41.0	34.0	41.0
13	37.96803055647268	40.0	35.0	41.0	34.0	41.0
14	37.93132930357593	39.0	35.0	41.0	34.0	41.0
15	37.88875240776179	39.0	35.0	41.0	34.0	41.0
16	37.80355010882849	39.0	35.0	41.0	34.0	41.0
17	37.76320141954281	39.0	35.0	41.0	34.0	41.0
18	37.74716897284999	39.0	35.0	41.0	34.0	41.0
19	37.695929611206125	39.0	35.0	41.0	34.0	41.0
20	37.557373970980116	39.0	35.0	41.0	33.0	41.0
21	37.404586913355	38.0	35.0	40.0	33.0	41.0
22	37.25732342949776	38.0	35.0	40.0	33.0	41.0
23	37.07108197891028	38.0	35.0	40.0	33.0	41.0
24	36.86523105907094	38.0	35.0	40.0	33.0	41.0
25	36.67492700586314	37.0	35.0	40.0	33.0	41.0
26	36.4256256963849	37.0	35.0	40.0	32.0	41.0
27	36.229834337084824	37.0	35.0	40.0	32.0	41.0
28	36.1399409628343	36.0	35.0	39.0	32.0	41.0
29	35.93603443786212	36.0	34.0	39.0	31.0	41.0
30	35.827853705928305	36.0	34.0	39.0	31.0	41.0
31	35.73511602853592	36.0	34.0	39.0	31.0	41.0
32	35.58443112173813	35.0	34.0	39.0	31.0	41.0
33	35.50221135920845	35.0	34.0	39.0	31.0	41.0
34	35.37037532649238	35.0	34.0	38.0	31.0	41.0
35	35.24489667091482	35.0	34.0	38.0	31.0	40.0
36	35.15517892777287	35.0	34.0	38.0	31.0	40.0
37	35.12444890888281	35.0	34.0	38.0	31.0	40.0
38	34.993944770602305	35.0	34.0	37.0	31.0	40.0
39	34.8788358889879	35.0	34.0	37.0	30.0	40.0
40	34.65322414076168	35.0	33.0	37.0	30.0	40.0
41	34.52354521230109	35.0	33.0	36.0	30.0	40.0
42	34.383409992012595	35.0	33.0	36.0	30.0	39.0
43	34.26110855195812	35.0	33.0	36.0	29.0	39.0
44	33.99969547680299	35.0	33.0	36.0	29.0	39.0
45	33.85110818973733	35.0	32.0	36.0	29.0	39.0
46	33.5507521317544	35.0	32.0	36.0	28.0	39.0
47	33.41695628025074	35.0	32.0	35.0	28.0	38.0
48	34.06996039504688	35.0	33.0	36.0	30.0	39.0
49	34.626198157868856	35.0	34.0	36.0	31.0	39.0
50	34.370020215987914	35.0	33.0	36.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	6.0
22	23.0
23	61.0
24	151.0
25	306.0
26	565.0
27	1204.0
28	2374.0
29	4708.0
30	8422.0
31	14799.0
32	26358.0
33	45205.0
34	73822.0
35	66551.0
36	98363.0
37	112359.0
38	86624.0
39	61940.0
40	35234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.78964415979871	18.977622484857115	35.23273335534417
2	23.301501066378336	19.84934569904503	34.37634470855716	22.472808526019474
3	20.309069770716924	21.490457361296492	30.82154162474436	27.378931243242228
4	17.62489457484912	23.26738947767021	35.55554164665089	23.552174300829787
5	16.91778324745454	28.664218351721775	32.091963591355686	22.326034809468002
6	19.57520118795955	28.28992972699776	28.724148344727336	23.410720740315362
7	24.799437941162203	26.406594489883094	23.943518719907868	24.85044884904683
8	24.909752941342152	26.467306858776457	26.474504716944224	22.148435482937163
9	24.152100131595624	25.88115084363592	26.03136701409372	23.935382010674736
10	24.955600168367294	22.97931867578187	28.054121634414525	24.010959521436316
11	27.119182448492285	24.580059742222794	25.743296212205376	22.55746159707955
12	25.18812228222176	24.056650273283896	27.633203406777568	23.122024037716777
13	23.68752532941937	23.84071452825081	27.753376343143803	24.718383799186018
14	20.843432502085033	26.329608702523476	25.394825991778795	27.432132803612696
15	21.652252694893743	31.0014880789386	25.031490629483994	22.314768596683663
16	22.555583894948825	32.69110704623372	23.141270484556685	21.612038574260772
17	21.156382857205447	33.3828838062274	21.840492333498677	23.62024100306848
18	20.75189452321231	28.600689429632332	22.495810377120826	28.151605670034535
19	23.717725038688485	25.292647700831978	23.23672034286841	27.752906917611124
20	21.65491277291227	26.052491163064346	26.194414149111456	26.098181914911926
21	23.56985599589409	29.318128118745886	23.401332229661747	23.71068365569828
22	26.012120567253817	27.51584702360741	22.759940476842456	23.71209193229632
23	25.133825395608365	25.79790604917389	26.11555065962111	22.952717895596635
24	25.760977907269677	26.3031643975158	24.72965001197035	23.206207683244166
25	25.338527525282334	27.32113485224098	24.571046314878213	22.769291307598476
26	25.442476824152017	31.115467946879004	23.36762624477563	20.07442898419335
27	26.639261136690674	28.307389112210952	23.215007242661915	21.838342508436465
28	27.06815376732525	26.338084450216392	23.70187520342364	22.89188657903472
29	24.195853134561553	28.29031313065556	24.405637653798582	23.10819608098431
30	25.620006958257026	30.014211911748294	24.97800928205229	19.387771847942393
31	23.958821163975635	29.2658669415894	24.34224570612503	22.433066188309937
32	22.333980187248358	28.50323121050673	25.9024112689405	23.26037733330441
33	25.072157046007366	25.494424954150645	28.49854509206012	20.934872907781866
34	24.8301900854724	28.31514687498941	24.617271319825328	22.237391719712857
35	22.04006913059575	27.547825849588065	26.179886272322	24.232218747494187
36	23.527138838609517	27.949758029338728	25.96006883026333	22.563034301788417
37	23.041919232085863	31.289181497861335	23.056960777872675	22.611938492180126
38	25.57390737116765	28.06244835833877	22.72511415525114	23.638530115242443
39	22.81987405837011	32.1475026128424	22.632554703378926	22.400068625408558
40	21.38876286275666	30.396796955130185	25.454179546341233	22.760260635771925
41	22.022915603942455	31.128693375378568	24.45288282150157	22.395508199177407
42	20.99624429567472	33.909340218490755	23.69285081938542	21.4015646664491
43	23.378045392062237	28.07445672837881	24.90823608551958	23.639261794039367
44	23.29303538634279	29.240765754404848	24.010626551807242	23.455572307445124
45	23.0523901881142	27.535257902938337	27.423643084106093	21.98870882484137
46	22.31994671438338	30.33499154772294	25.60237587023282	21.742685867660864
47	22.92866777751329	30.978867465418286	24.796477029278744	21.29598772778968
48	24.408119015390785	28.313154860380006	23.615393292224397	23.663332832004812
49	18.977603169177314	28.839607224300774	24.602799252658542	27.57999035386337
50	22.569760188782755	29.694205000638796	25.494690484958255	22.241344325620194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15840.0
1	13517.5
2	10772.5
3	9927.5
4	8933.0
5	6365.5
6	3362.0
7	2365.0
8	2229.5
9	2003.5
10	1797.5
11	1660.5
12	1653.0
13	1626.5
14	1573.0
15	1446.0
16	1317.0
17	1412.0
18	1628.0
19	1809.0
20	1904.0
21	2146.5
22	2512.0
23	2920.0
24	3699.5
25	4943.5
26	5845.0
27	6062.5
28	6405.5
29	7695.5
30	9398.0
31	10422.0
32	12441.0
33	16021.5
34	18857.5
35	19717.0
36	20783.5
37	22515.5
38	23473.0
39	26030.0
40	29377.0
41	33529.5
42	37927.5
43	40085.5
44	40317.0
45	39024.5
46	41784.5
47	45680.0
48	47637.0
49	53706.5
50	63005.5
51	67763.0
52	65051.5
53	59572.0
54	56805.0
55	54433.5
56	50058.0
57	48350.5
58	46758.5
59	39165.0
60	30116.5
61	24407.0
62	20614.5
63	17005.0
64	12537.5
65	9199.5
66	6457.0
67	4245.5
68	3277.5
69	2624.5
70	2139.0
71	1609.0
72	1034.0
73	749.0
74	671.5
75	636.5
76	603.0
77	452.0
78	297.5
79	172.5
80	65.5
81	36.0
82	27.5
83	23.0
84	17.5
85	15.0
86	11.5
87	8.0
88	6.0
89	4.0
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3754.0
25	4388.0
26	4786.0
27	5525.0
28	6186.0
29	5092.0
30	5057.0
31	4980.0
32	4428.0
33	4517.0
34	4229.0
35	3835.0
36	3904.0
37	3523.0
38	3658.0
39	4756.0
40	5795.0
41	6043.0
42	7567.0
43	11793.0
44	26370.0
45	53986.0
46	76817.0
47	58938.0
48	105595.0
49	80494.0
50	133063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.082609505241136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.99297769975222	9.575185540441792
2	11.227902532250358	3.1623633384917986
3	4.418937988199869	1.8669053434708385
4	2.395582173135257	1.3494419312792316
5	1.4544606051178346	1.0241300371315596
6	1.0722341359348437	0.9059912780736028
7	0.7977866420738009	0.7864442424176041
8	0.642229358103979	0.7235412210384006
9	0.5377837531528128	0.6816058734522649
>10	5.762286247624973	19.399166613204315
>50	2.24224713607929	22.478285157234083
>100	1.4066822964699608	32.045490463620304
>500	0.04111156790631007	3.820028509777351
>1k	0.007777864198491094	2.1814204503668564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	4779	0.7477948735602328	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	3009	0.4708338092786651	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	1797	0.2811858940756933	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1202	0.1880831634273697	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	1064	0.16648958892406104	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1063	0.1663331137465008	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1027	0.16070000735433335	No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC	889	0.13910643285102467	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.13785463143054302	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	871	0.13628987965494094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	849	0.13284742574861638	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	811	0.12690136900132848	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	802	0.1254930924032866	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACCC	796	0.12455424133792536	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	786	0.12298948956232328	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	736	0.11516573068431289	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	726	0.11360097890871082	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	722	0.11297507819846997	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	716	0.11203622713310873	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	716	0.11203622713310873	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	715	0.11187975195554854	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.10781139733898314	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	681	0.10655959591850148	No Hit
TCTCATTGAACTCGCCTGCAGCTCTTGGGTTTTTTGTGGCTT	658	0.1029606668346167	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA	656	0.10264771647949626	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.10249124130193607	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT	643	0.10061353917121359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0627465462016433	0.0	0.0	0.0	0.0
2	0.12205063849696203	0.0	0.0	0.0	0.0
3	0.15850935486849044	0.0	0.0	0.0	0.0
4	0.18777021307224928	0.0	0.0	0.0	0.0
5	0.22626310675206038	0.0	0.0	0.0	0.0
6	0.28024704301033204	0.0	0.0	0.0	0.0
7	0.3104467522794522	0.0	0.0	0.0	0.0
8	0.3459666175856193	0.0	0.0	0.0	0.0
9	0.3742886247240169	0.0	0.0	0.0	0.0
10	0.40605308576873905	0.0	0.0	0.0	0.0
11	0.43547041915005813	0.0	0.0	0.0	0.0
12	0.46175824898017304	0.0	0.0	0.0	0.0
13	0.48961083058589	0.0	0.0	0.0	0.0
14	0.5210623412754918	0.0	0.0	0.0	0.0
15	0.5518879512548527	0.0	0.0	0.0	0.0
16	0.5816182349912922	0.0	0.0	0.0	0.0
17	0.613852121568695	0.0	0.0	0.0	0.0
18	0.6465554336787783	0.0	0.0	0.0	0.0
19	0.6767551429478985	0.0	0.0	0.0	0.0
20	0.7093019798804217	0.0	0.0	0.0	0.0
21	0.7415358664578244	0.0	0.0	0.0	0.0
22	0.7714226253718242	0.0	0.0	0.0	0.0
23	0.7986493062673002	0.0	0.0	0.0	0.0
24	0.823998285032054	0.0	0.0	0.0	0.0
25	0.8527897177031322	0.0	0.0	0.0	0.0
26	0.8854930298132155	0.0	0.0	0.0	0.0
27	0.9105290582228488	0.0	0.0	0.0	0.0
28	0.9366604128754035	0.0	0.0	0.0	0.0
29	0.9720238030040105	0.0	0.0	0.0	0.0
30	1.0053530158243347	0.0	0.0	0.0	0.0
31	1.035865675448575	0.0	0.0	0.0	0.0
32	1.0638747322318525	0.0	0.0	0.0	0.0
33	1.0957956684541348	0.0	0.0	0.0	0.0
34	1.1256824273681345	0.0	0.0	0.0	0.0
35	1.1541609096840924	0.0	0.0	0.0	0.0
36	1.1782580870283643	0.0	0.0	0.0	0.0
37	1.194531505494626	0.0	0.0	0.0	0.0
38	1.208144845942364	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	10	0.0031918192	189.29195	44
CATATAC	15	4.243203E-5	189.29195	44
CAACGGT	20	5.631464E-7	189.29195	44
AACGGTG	20	5.631464E-7	189.29195	44
GATATAC	10	0.0031918192	189.29195	44
GCTAGTT	20	5.631464E-7	189.29195	44
ACGAACA	85	0.0	167.02231	44
ACCTGCC	45	1.0913936E-11	147.22708	44
AGAATCG	35	5.606853E-8	135.20856	44
AATCGCA	90	0.0	126.19464	44
GGGCGTA	30	2.5393092E-9	119.17063	43
GGCATCG	15	2.683298E-4	119.17063	43
TAACTAA	15	2.683298E-4	119.17063	43
CGCCAGT	30	2.5393092E-9	119.17063	43
GTTCGGC	50	0.0	119.17062	43
GAGTTAT	25	1.193348E-7	119.17062	43
TAACGGT	20	5.643409E-6	119.170616	43
AGACCGC	20	5.643409E-6	119.170616	43
GACGATT	20	5.643409E-6	119.170616	43
AGAGCCA	165	0.0	114.7224	44
>>END_MODULE