FastQCFastQC Report
Tue 24 May 2016
ERR840988.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840988.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4919161
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC850601.7291566590318959No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT629971.2806452157186967No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA561221.140885610371362No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC367430.7469363169857625No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT314000.6383202338772811No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA224270.4559110791454071No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG161960.32924313719351733No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA142200.2890736855329598No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA141940.2885451401163735No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA106830.21717118020735648No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA97730.19867209062683658No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC90870.18472662309690616No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG84630.17204153309883535No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC84490.1717569317206735No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG82030.1667560789329725No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG73070.14854159073061443No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG72590.14756581457691667No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC71930.14622412236558224No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC65520.13319344497974347No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT65030.13219734015617704No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG63140.12835522155099213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA57930.11776398454939777No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC57640.11717445312320535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA55540.11290543245077768No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG55230.1122752436848479No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT55050.11190932762721122No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT51560.1048146218430338No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT50000.1016433493435161No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA49750.10113513259679852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT94750.0176.201544
ACACTCG402.2038876E-8158.2173344
AATCGCA5950.0148.9104344
ACGGATA352.1556443E-6144.6558544
CGTGCGC2400.0137.1216944
ACTTCTA35600.0117.6852644
TAATTCC11100.0117.4514244
GTCGTTG953.6379788E-12106.5885244
ACATTGG20800.0102.8412744
CCTTAAC115600.094.048743
CATGACC13050.090.2020644
GGTCGAG851.8855644E-889.3462544
TCGTGCG6300.084.1583543
CTAATTC7450.083.1619843
CCTATCA95850.082.41366643
CCCTATC115550.073.4811542
CAGGCAC17200.072.8535644
GAACACA35150.072.7394644
TGCGGCA7050.070.0195944
AACGACA5850.069.2469743
Produced by FastQC (version 0.11.5)