##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840988.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4919161
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.345194028006	39.0	37.0	39.0	34.0	39.0
2	37.884541083327015	39.0	38.0	39.0	35.0	39.0
3	38.09362734824089	39.0	38.0	39.0	35.0	39.0
4	38.667680321908556	39.0	38.0	41.0	35.0	41.0
5	39.25341780844335	40.0	39.0	41.0	35.0	41.0
6	39.25043173012634	40.0	39.0	41.0	35.0	41.0
7	39.28932149201866	40.0	39.0	41.0	36.0	41.0
8	39.24888675121632	40.0	39.0	41.0	35.0	41.0
9	39.286378713768464	40.0	39.0	41.0	35.0	41.0
10	39.26285986573727	40.0	39.0	41.0	35.0	41.0
11	39.14488852875521	40.0	39.0	41.0	35.0	41.0
12	39.11274280309183	40.0	38.0	41.0	35.0	41.0
13	39.01411094290266	40.0	38.0	41.0	35.0	41.0
14	38.942636559364495	40.0	38.0	41.0	35.0	41.0
15	38.91822203013888	40.0	38.0	41.0	35.0	41.0
16	38.89177646350668	40.0	38.0	41.0	35.0	41.0
17	38.88072254597888	40.0	38.0	41.0	35.0	41.0
18	38.81867070421155	40.0	38.0	41.0	35.0	41.0
19	38.80204856072001	40.0	38.0	41.0	35.0	41.0
20	38.67268707001052	40.0	38.0	41.0	35.0	41.0
21	38.600670114273555	40.0	38.0	41.0	35.0	41.0
22	38.56039759625676	40.0	38.0	41.0	35.0	41.0
23	38.45080472056109	40.0	38.0	41.0	35.0	41.0
24	38.271936820120345	40.0	37.0	41.0	34.0	41.0
25	38.17928125089399	40.0	37.0	41.0	34.0	41.0
26	38.103004295372216	40.0	37.0	41.0	34.0	41.0
27	38.02572957624499	39.0	37.0	41.0	34.0	41.0
28	37.86157712258893	39.0	36.0	41.0	33.0	41.0
29	37.86244507307755	39.0	36.0	41.0	33.0	41.0
30	37.82243788422819	39.0	36.0	41.0	33.0	41.0
31	37.74637144442773	39.0	36.0	41.0	33.0	41.0
32	37.72819726851161	39.0	36.0	41.0	33.0	41.0
33	37.561975209357186	39.0	36.0	41.0	33.0	41.0
34	37.484161577543794	39.0	35.0	41.0	33.0	41.0
35	37.36027452898725	38.0	35.0	41.0	32.0	41.0
36	37.34893802752749	38.0	35.0	41.0	32.0	41.0
37	37.263209794605146	38.0	35.0	40.0	32.0	41.0
38	37.059561064083056	38.0	35.0	40.0	32.0	41.0
39	37.071428047532315	38.0	35.0	40.0	32.0	41.0
40	36.77608611459113	38.0	35.0	40.0	31.0	41.0
41	36.73038068688419	38.0	35.0	40.0	31.0	41.0
42	36.59246284225851	38.0	34.0	40.0	31.0	41.0
43	36.47679433967597	38.0	34.0	40.0	31.0	41.0
44	36.159815040317795	37.0	34.0	40.0	30.0	41.0
45	35.967592899304925	37.0	34.0	40.0	29.0	41.0
46	35.624880409844145	37.0	33.0	40.0	28.0	41.0
47	35.951751611206106	37.0	34.0	40.0	30.0	41.0
48	36.807152779984	38.0	35.0	40.0	32.0	41.0
49	37.350846799504396	39.0	35.0	40.0	33.0	41.0
50	37.17967925749942	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	13.0
21	33.0
22	81.0
23	184.0
24	410.0
25	761.0
26	1404.0
27	2776.0
28	6002.0
29	12641.0
30	25825.0
31	50814.0
32	95199.0
33	166615.0
34	263888.0
35	280003.0
36	465312.0
37	797902.0
38	820898.0
39	763335.0
40	1165058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.122112083747616	17.04123934955575	31.836648566696642
2	26.822480500231645	22.191650161480787	31.18852991394264	19.797339424344923
3	24.42833239245473	26.60571589342166	23.9891314799414	24.976820234182213
4	22.042762983362408	26.470225308746752	26.812112878598604	24.67489882929223
5	17.648558361883257	27.35334338518296	26.93764241503785	28.06045583789593
6	19.982655578868023	27.65105675541012	25.961500345282456	26.4047873204394
7	24.622593161720058	23.502605423973723	24.070974704832796	27.803826709473423
8	20.331068651747728	24.157859439851634	29.076604729952933	26.434467178447708
9	21.352543655310328	27.934479070719583	28.589489142559067	22.123488131411026
10	29.087907470399934	20.294883619381434	27.2948578019707	23.32235110824793
11	23.135286688116125	23.48835502639576	24.832994894861134	28.54336339062698
12	22.742089555515665	25.423298810508538	25.637034445508082	26.197577188467708
13	24.36206092868276	24.977430094278272	23.33143802367924	27.32907095335973
14	19.675469048482046	23.927596596248833	25.21850779025122	31.178426565017897
15	21.977182694365972	29.238949487524398	25.48554519764651	23.298322620463125
16	20.708917638597313	30.97493657963218	22.85267751960141	25.463468262169098
17	21.94597818611751	33.72705223512709	20.354466950766604	23.9725026279888
18	22.409166929075912	27.923338959631533	21.671378513531067	27.996115597761488
19	25.87394476415795	23.496730438381665	24.318049358417014	26.311275439043364
20	23.884011928050334	22.70267226464025	27.296707710928754	26.116608096380663
21	22.485806014480925	31.834473399020684	22.17235825377539	23.507362332723
22	26.09676731458881	26.67361365078313	21.006427722125785	26.223191312502276
23	27.6119647232526	24.720800965855762	26.10223572678349	21.564998584108146
24	22.489139916339393	27.219275807398862	24.188108500616266	26.103475775645478
25	21.300119743838096	25.975074482302023	24.994155846809623	27.730649927050255
26	22.770546922971523	32.19448140503805	26.267426517580745	18.767545154409685
27	23.8319838424707	28.28852660058872	22.27814799939839	25.60134155754219
28	24.053581707653866	25.858077600061225	22.097297041002726	27.991043651282187
29	23.469318142343127	29.69956610922563	26.313736470472033	20.51737927795921
30	26.138891747592535	29.24542219825822	25.4485726931156	19.167113361033643
31	25.02043957407497	29.770537097614476	23.22819488390237	21.980828444408182
32	21.778576326146183	28.362946643479418	24.69170385023244	25.166773180141956
33	21.32487697678444	24.957182437757293	30.02611521875393	23.691825366704332
34	23.50558755215027	29.092966786799796	24.780299896207396	22.621145764842538
35	20.481625303398125	24.991508438211092	24.79988056398313	29.726985694407652
36	21.52045502927205	24.00389128360341	27.776823388966186	26.69883029815835
37	19.32904932904933	33.66814204023507	22.99319159784276	24.009617032872846
38	24.418736509136487	24.695201811713137	24.693676577374145	26.19238510177623
39	22.496102132464152	29.1448917654145	27.17283194662227	21.186174155499078
40	18.26828245016046	24.876572923836854	29.923973714585482	26.9311709114172
41	20.23139171359886	29.580691398711107	24.943647506292113	25.24426938139792
42	21.02044102176455	30.2307489037676	23.44534210956002	25.303467964907828
43	20.339055100704805	23.187323830066198	24.94087170037893	31.532749368850066
44	19.090290577915777	26.553244484289962	23.538025611894138	30.818439325900123
45	19.601299886829356	24.08591937317208	32.556051230250034	23.75672950974853
46	19.60348721533622	29.492164744261913	27.877417569614355	23.026930470787512
47	21.473859003025122	29.11334341707221	27.647145863474943	21.765651716427726
48	19.281545042065833	29.782345264535522	23.674908744931823	27.26120094846683
49	19.129811802752318	28.763291723052113	23.117678120786284	28.989218353409285
50	18.92507151830327	27.84789881933535	28.94537265614722	24.28165700621416
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72795.0
1	67181.5
2	53786.0
3	44065.5
4	39126.5
5	27195.5
6	13730.5
7	9389.5
8	9154.0
9	7965.0
10	6426.0
11	5360.5
12	5150.0
13	5471.0
14	5979.5
15	6294.0
16	6477.5
17	6754.5
18	6928.5
19	7532.5
20	8859.0
21	10899.0
22	13410.5
23	17294.5
24	21087.5
25	23575.5
26	26670.5
27	31223.5
28	36975.5
29	43403.5
30	52937.5
31	74128.5
32	112130.0
33	135593.5
34	142649.0
35	155573.0
36	162027.5
37	167375.0
38	176361.5
39	186707.0
40	203523.0
41	233247.5
42	271753.5
43	309661.0
44	343041.5
45	371291.0
46	395106.5
47	398434.5
48	479389.5
49	603656.5
50	604422.5
51	563215.0
52	536468.0
53	471825.0
54	402276.0
55	367284.5
56	344777.0
57	314406.5
58	270601.0
59	225395.0
60	194726.5
61	168892.5
62	144166.5
63	119547.0
64	98854.0
65	84071.5
66	65206.0
67	44735.0
68	31502.5
69	24636.5
70	19443.5
71	14730.5
72	11657.5
73	8561.0
74	5334.5
75	3385.5
76	2486.0
77	1839.5
78	1433.0
79	1122.0
80	711.5
81	362.5
82	222.0
83	148.0
84	135.5
85	168.0
86	193.5
87	163.0
88	92.0
89	48.0
90	38.0
91	26.5
92	21.0
93	21.0
94	11.5
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	25381.0
25	25983.0
26	27453.0
27	31688.0
28	30034.0
29	27051.0
30	45015.0
31	26397.0
32	29614.0
33	24011.0
34	24907.0
35	26273.0
36	25094.0
37	26365.0
38	24640.0
39	34878.0
40	34227.0
41	32816.0
42	45357.0
43	86615.0
44	235165.0
45	459669.0
46	529328.0
47	617499.0
48	778034.0
49	870341.0
50	775326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.941912367834474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08642091874721	7.8876330060376185
2	12.236332222655502	2.6777774976801147
3	4.173264122106436	1.3699047093574885
4	1.8825021029070905	0.8239269216909401
5	1.0764578335314963	0.5889253641085291
6	0.7253066565785335	0.47617451256535964
7	0.5216528674899374	0.3995515973753124
8	0.3922780966973372	0.34338180463065293
9	0.3211683005070282	0.31627758595268085
>10	3.04265233737087	7.636191564809473
>50	1.528161106850406	12.452833162665442
>100	1.8955112762155293	35.052934736334684
>500	0.061840984457242586	4.647216994661295
>1k	0.04879196938828314	10.787180180260682
>5k	0.0048224620907024035	3.6750013206913437
>10k+	0.0028367424062955317	10.865089041178393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	85060	1.7291566590318959	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	62997	1.2806452157186967	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	56122	1.140885610371362	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	36743	0.7469363169857625	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	31400	0.6383202338772811	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	22427	0.4559110791454071	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	16196	0.32924313719351733	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	14220	0.2890736855329598	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	14194	0.2885451401163735	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	10683	0.21717118020735648	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9773	0.19867209062683658	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	9087	0.18472662309690616	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	8463	0.17204153309883535	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	8449	0.1717569317206735	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	8203	0.1667560789329725	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	7307	0.14854159073061443	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	7259	0.14756581457691667	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	7193	0.14622412236558224	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	6552	0.13319344497974347	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	6503	0.13219734015617704	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	6314	0.12835522155099213	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5793	0.11776398454939777	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	5764	0.11717445312320535	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5554	0.11290543245077768	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	5523	0.1122752436848479	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	5505	0.11190932762721122	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	5156	0.1048146218430338	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	5000	0.1016433493435161	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4975	0.10113513259679852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0473454721242098	0.0	0.0	0.0	0.0
2	0.09139769972968968	0.0	0.0	0.0	0.0
3	0.12134183044628952	0.0	0.0	0.0	0.0
4	0.1490294788074633	0.0	0.0	0.0	0.0
5	0.17328158196082624	0.0	0.0	0.0	0.0
6	0.21259722948689827	0.0	0.0	0.0	0.0
7	0.24097605262360797	0.0	0.0	0.0	0.0
8	0.275087560663292	0.0	0.0	0.0	0.0
9	0.3009659574061512	0.0	0.0	0.0	0.0
10	0.3268240254791417	0.0	0.0	0.0	0.0
11	0.35367819837569864	0.0	0.0	0.0	0.0
12	0.3877287204057765	0.0	0.0	0.0	0.0
13	0.4160262288630114	0.0	0.0	0.0	0.0
14	0.4439374925927409	0.0	0.0	0.0	0.0
15	0.4736173506010476	0.0	0.0	0.0	0.0
16	0.5132582568450189	0.0	0.0	0.0	0.0
17	0.5512931981693626	0.0	0.0	0.0	0.0
18	0.5980288101975113	0.0	0.0	0.0	0.0
19	0.6248423257543309	0.0	0.0	0.0	0.0
20	0.6490334428980877	0.0	0.0	0.0	0.0
21	0.6744646089038354	0.0	0.0	0.0	0.0
22	0.6975579778746823	0.0	0.0	0.0	0.0
23	0.7236599899860973	0.0	0.0	0.0	0.0
24	0.7496806874180374	0.0	0.0	0.0	0.0
25	0.7764332169652508	0.0	0.0	0.0	0.0
26	0.808959088755176	0.0	0.0	0.0	0.0
27	0.8341259820526306	0.0	0.0	0.0	0.0
28	0.8611631129780059	0.0	0.0	0.0	0.0
29	0.8899688381819583	0.0	0.0	0.0	0.0
30	0.9196283675203962	0.0	0.0	0.0	0.0
31	0.9469704284938021	0.0	0.0	0.0	0.0
32	0.9759794403964416	0.0	0.0	0.0	0.0
33	1.0042769488536765	0.0	0.0	0.0	0.0
34	1.0319239398751128	0.0	0.0	0.0	0.0
35	1.0561557143586071	0.0	0.0	0.0	0.0
36	1.0760574821600675	0.0	0.0	0.0	0.0
37	1.093967040314395	0.0	0.0	0.0	0.0
38	1.1075669204565575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	9475	0.0	176.2015	44
ACACTCG	40	2.2038876E-8	158.21733	44
AATCGCA	595	0.0	148.91043	44
ACGGATA	35	2.1556443E-6	144.65585	44
CGTGCGC	240	0.0	137.12169	44
ACTTCTA	3560	0.0	117.68526	44
TAATTCC	1110	0.0	117.45142	44
GTCGTTG	95	3.6379788E-12	106.58852	44
ACATTGG	2080	0.0	102.84127	44
CCTTAAC	11560	0.0	94.0487	43
CATGACC	1305	0.0	90.20206	44
GGTCGAG	85	1.8855644E-8	89.34625	44
TCGTGCG	630	0.0	84.15835	43
CTAATTC	745	0.0	83.16198	43
CCTATCA	9585	0.0	82.413666	43
CCCTATC	11555	0.0	73.48115	42
CAGGCAC	1720	0.0	72.85356	44
GAACACA	3515	0.0	72.73946	44
TGCGGCA	705	0.0	70.01959	44
AACGACA	585	0.0	69.24697	43
>>END_MODULE