FastQCFastQC Report
Tue 24 May 2016
ERR840987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3887528
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA176490.4539902992338576No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG173960.4474823075229298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA166350.4279068858153562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA162770.41869794892795625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA131430.3380811662321146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA129880.334094056685894No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA128470.33046707316320295No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC120960.3111488843295791No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA100800.25929073694131594No Hit
AAAAAAAAAAAAAAAAAAAAAAAA100580.2587248246186265No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG100100.2574901068236679No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT90510.23282147421188992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84290.21682158945221744No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA83670.2152267456337292No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA80090.2060178087463293No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG72800.18726553223539485No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA70870.18230093776816528No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA70460.18124628298497142No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG67480.17358074334126983No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC66920.17214023924715138No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA56360.14497644775806118No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA52150.13414694376477804No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA51770.1331694588437691No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC51330.13203763419839035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48040.12357467264544461No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC47690.1226743575866206No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47390.12190265896477144No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG47070.12107951376813236No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA46930.12071938774460274No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT46410.1193817768000642No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG45360.11668083162359216No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG45140.11611491930090279No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA44360.11410850288409498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1000.0169.966944
TTTACGC2900.0153.285844
TACGGTA557.2941475E-10142.6295644
CTATCAT19750.0116.5109144
ACGACAC3650.0100.2965944
CTATGCG1055.456968E-1299.61429644
ACTCGAC800.098.7198943
AACGACA5300.091.8902143
CCGGGCT4600.090.95219444
TACGCGA257.2930015E-787.99248542
ATGTTCT7500.083.6760244
CCTTCGT1109.440555E-1083.20058444
CTCGACA1750.082.181844
TCCGGGC6850.079.7445343
GTACGGC250.001378524678.9759143
CGTGCGC1501.8189894E-1278.44626644
TATTCGT500.001415485378.44626644
CAATGAA28000.076.3903943
CCGAAAC355.5538276E-575.2151643
TGCGTAT355.5538276E-575.2151643
Produced by FastQC (version 0.11.5)