##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840987.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3887528
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.77978576617326	38.0	35.0	39.0	30.0	39.0
2	36.88341743133426	39.0	35.0	39.0	33.0	39.0
3	36.8258072996516	39.0	35.0	39.0	33.0	39.0
4	37.270100434003304	39.0	35.0	39.0	33.0	41.0
5	37.9478262278754	39.0	35.0	41.0	34.0	41.0
6	37.980113069282076	39.0	35.0	41.0	34.0	41.0
7	38.03155244155155	39.0	36.0	41.0	34.0	41.0
8	37.8874547012909	39.0	35.0	41.0	34.0	41.0
9	37.994630520989176	39.0	35.0	41.0	34.0	41.0
10	38.00824997273332	39.0	36.0	41.0	34.0	41.0
11	37.88580275177439	39.0	35.0	41.0	34.0	41.0
12	37.84805897217975	39.0	35.0	41.0	34.0	41.0
13	37.6293536663916	39.0	35.0	40.0	34.0	41.0
14	37.55424166719828	39.0	35.0	40.0	33.0	41.0
15	37.29053038331814	38.0	35.0	40.0	33.0	41.0
16	37.3251408092752	38.0	35.0	40.0	33.0	41.0
17	37.391627790204986	38.0	35.0	40.0	33.0	41.0
18	37.41925357193569	38.0	35.0	40.0	33.0	41.0
19	37.40110887947302	38.0	35.0	40.0	33.0	41.0
20	37.29748133003801	38.0	35.0	40.0	33.0	41.0
21	37.046056517149204	38.0	35.0	40.0	33.0	41.0
22	37.01441584472189	38.0	35.0	40.0	33.0	41.0
23	36.95372689277093	38.0	35.0	40.0	33.0	41.0
24	36.7474454203288	38.0	35.0	40.0	33.0	41.0
25	36.56951146732979	37.0	35.0	40.0	32.0	41.0
26	36.341078021205256	37.0	35.0	40.0	32.0	41.0
27	36.18650815461531	37.0	35.0	40.0	32.0	41.0
28	36.02300376744802	36.0	34.0	40.0	31.0	41.0
29	35.9127168670216	36.0	34.0	39.0	31.0	41.0
30	35.83342564197333	36.0	34.0	39.0	31.0	41.0
31	35.691589739377704	35.0	34.0	39.0	31.0	41.0
32	35.57551655567046	35.0	34.0	39.0	31.0	40.0
33	35.41460663893914	35.0	34.0	39.0	31.0	40.0
34	35.29836385005038	35.0	34.0	38.0	31.0	40.0
35	35.1483756935538	35.0	34.0	38.0	31.0	40.0
36	35.11709646078469	35.0	34.0	38.0	31.0	40.0
37	35.013381698763276	35.0	34.0	38.0	31.0	40.0
38	34.89324888701257	35.0	34.0	37.0	30.0	40.0
39	34.783668015607816	35.0	33.0	37.0	30.0	40.0
40	34.55924148564059	35.0	33.0	37.0	30.0	40.0
41	34.435659154809834	35.0	33.0	37.0	29.0	40.0
42	34.307442870239036	35.0	33.0	36.0	29.0	40.0
43	34.18192271985706	35.0	33.0	36.0	29.0	40.0
44	33.93281412163203	35.0	32.0	36.0	29.0	39.0
45	33.76948391891362	35.0	32.0	36.0	28.0	39.0
46	33.582637080913706	35.0	32.0	36.0	27.0	39.0
47	33.50506293404931	35.0	32.0	35.0	27.0	39.0
48	34.22135755895595	35.0	33.0	36.0	29.0	39.0
49	34.52737482086246	35.0	33.0	37.0	30.0	39.0
50	35.009305341431535	35.0	34.0	38.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	29.0
22	93.0
23	209.0
24	569.0
25	1583.0
26	3516.0
27	7579.0
28	15916.0
29	30589.0
30	57772.0
31	105773.0
32	190646.0
33	320857.0
34	480925.0
35	380761.0
36	609667.0
37	659794.0
38	493862.0
39	346101.0
40	181281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.06366204950807	19.08948308539514	26.84685486509679
2	32.30626248865603	23.202353783689787	25.206146425183306	19.285237302470875
3	25.177747915899257	22.242437867971628	28.852653923007114	23.727160293122004
4	23.994759651891897	24.4684539892703	28.221584513346272	23.315201845491533
5	18.926037317287488	25.323804741727905	27.95869251616966	27.791465424814948
6	21.988446128233676	31.6822927063162	27.169604952041503	19.159656213408624
7	30.567265367606357	23.91375701988513	23.812690223710288	21.70628738879823
8	21.34592985568207	24.076045240060008	29.86918679428161	24.708838109976313
9	26.94015322847835	25.418569332490982	26.68351199013872	20.957765448891944
10	25.25206249318333	22.618203650237376	26.799524016290043	25.330209840289253
11	24.443270890910625	24.716426479757832	30.410610547370975	20.429692081960567
12	24.88761495737137	24.76609814771752	29.202104782267806	21.144182112643303
13	23.6335532502917	28.726609814771752	25.52840776966751	22.111429165269033
14	25.279843643569897	25.210776616914398	24.81808491154276	24.691294827972943
15	22.17571166046907	27.07149633391708	29.68896944279244	21.063822562821414
16	23.143035882956983	29.32241259741409	22.438192085047362	25.096359434581565
17	29.245397074953544	28.89507162392142	20.315403516064706	21.544127785060326
18	27.005258868875025	28.03485917014617	22.70339917808952	22.256482782889282
19	28.555601400169977	26.27710977258556	23.003898621437582	22.163390205806877
20	27.76957490724182	27.86773497194104	23.172386153874648	21.19030396694249
21	25.071485015670625	31.287543137952962	22.91422724157871	20.7267446047977
22	24.95830255113275	33.73719751986352	20.79408817119774	20.510411757805986
23	26.36366863466964	31.656569418921226	21.321364116219872	20.658397830189262
24	24.005203306574256	32.94481737494881	22.007172681457213	21.04280663701972
25	23.782012627465903	31.466780182853004	24.122638960974317	20.62856822870677
26	23.68531893697442	33.72412129609088	24.12846427211044	18.46209549482426
27	25.44232049804987	32.14237017619888	22.677599382722573	19.737709943028676
28	26.42571226433389	31.40625982151096	21.69606042895679	20.471967485198356
29	26.545402885922837	31.604629924029737	22.931437346520454	18.91852984352697
30	27.27337591012401	31.111383268206993	22.13201247232791	19.483228349341093
31	24.462128687320956	30.738107949418342	24.021098629349392	20.77866473391131
32	24.56340637765205	30.594565547468545	24.937439563161483	19.904588511717925
33	24.255297608055482	29.657272734728345	25.846835348890874	20.2405943083253
34	22.852724264938594	29.911543433645317	24.581339610601784	22.654392690814305
35	22.447181884627586	29.018015160499957	25.03413181084771	23.50067114402475
36	24.287218039888693	28.70498394423967	25.227721981163864	21.780076034707772
37	22.099700745466762	31.07418577354977	24.83853471769797	21.98757876328549
38	23.19494664549326	29.930780159883934	24.072912821038216	22.801360373584586
39	23.709065692009187	30.48544663489055	24.583621289521247	21.221866383579016
40	21.103291430465216	29.966684638190006	25.772665433075687	23.15735849826909
41	22.557128369556636	31.215287266279233	24.460263674069218	21.767320690094916
42	22.663301010987876	32.027251112713806	23.15763553158484	22.151812344713477
43	21.884760049833965	29.349480521225523	25.423446524262182	23.342312904678327
44	21.483606270034468	30.472587493957697	24.69462060785462	23.34918562815321
45	22.127950215764226	28.151096145530342	25.579937260973313	24.141016377732115
46	22.188605431655482	29.18522803440682	25.82528717333799	22.800879360599712
47	23.95345002015345	28.319164489479803	25.94256951995097	21.784815970415774
48	22.958152646412604	29.303949314435823	24.170757241441322	23.567140797710252
49	20.09380292699874	30.4859512745907	24.505406696486734	24.91483910192383
50	22.111395076929444	29.12402868169731	26.377607858150455	22.386968383222793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134681.0
1	130757.0
2	88312.5
3	48292.0
4	43806.5
5	29783.5
6	13512.5
7	8637.0
8	8413.0
9	6914.5
10	5378.5
11	4675.0
12	4578.0
13	4608.5
14	5061.0
15	6037.5
16	7166.5
17	8821.0
18	11232.5
19	14333.0
20	17755.0
21	24008.0
22	34523.0
23	50323.0
24	68162.0
25	79444.0
26	83055.5
27	78248.0
28	66470.0
29	59160.0
30	59733.0
31	65680.0
32	75202.5
33	80567.0
34	87494.5
35	100501.5
36	112720.5
37	120708.0
38	128866.0
39	145328.5
40	162523.0
41	179201.5
42	206172.0
43	238953.0
44	260677.5
45	270346.0
46	278036.5
47	288890.0
48	312717.5
49	342869.0
50	372625.0
51	386013.5
52	373825.0
53	351656.0
54	325559.5
55	310662.0
56	299613.0
57	278742.5
58	245700.5
59	206578.5
60	178592.0
61	153497.0
62	128070.0
63	102401.5
64	82542.0
65	70886.0
66	55160.0
67	39278.0
68	28199.0
69	21602.0
70	16343.0
71	11087.5
72	7828.0
73	6021.0
74	4108.0
75	2844.5
76	2134.0
77	1428.5
78	957.5
79	674.0
80	559.0
81	431.5
82	294.0
83	198.0
84	131.0
85	106.5
86	88.5
87	71.5
88	39.5
89	24.0
90	20.0
91	14.0
92	10.5
93	8.5
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	30222.0
25	34858.0
26	38088.0
27	45755.0
28	43080.0
29	37507.0
30	54182.0
31	30891.0
32	36868.0
33	28819.0
34	26634.0
35	26412.0
36	26695.0
37	30803.0
38	28146.0
39	37372.0
40	37736.0
41	40602.0
42	52631.0
43	90898.0
44	200135.0
45	371441.0
46	421490.0
47	403586.0
48	561327.0
49	571468.0
50	579882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.340040698230945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7282939273723	8.851300662784459
2	11.794517783848619	2.910896589374012
3	4.174447253738883	1.5453854701126866
4	2.008097175886249	0.9912000350575575
5	1.200277332187324	0.7405735564177819
6	0.7917168150360566	0.5861890631411232
7	0.5915234877002227	0.5109596738526182
8	0.4408546210300215	0.4352131172410916
9	0.3554327604827834	0.39474492568579067
>10	3.5838725444010655	10.02796905278872
>50	1.5637862265326854	14.24307873425136
>100	1.6484039081592707	33.350356061318806
>500	0.0661577077183634	5.542178815418738
>1k	0.04523340946325312	10.792887841399876
>5k	0.0040002334899475545	3.4125052080194167
>10k+	0.003384812953032546	5.664561193135958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	17649	0.4539902992338576	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	17396	0.4474823075229298	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	16635	0.4279068858153562	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	16277	0.41869794892795625	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13143	0.3380811662321146	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12988	0.334094056685894	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	12847	0.33046707316320295	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12096	0.3111488843295791	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	10080	0.25929073694131594	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	10058	0.2587248246186265	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	10010	0.2574901068236679	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9051	0.23282147421188992	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8429	0.21682158945221744	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8367	0.2152267456337292	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	8009	0.2060178087463293	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	7280	0.18726553223539485	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7087	0.18230093776816528	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	7046	0.18124628298497142	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	6748	0.17358074334126983	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	6692	0.17214023924715138	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5636	0.14497644775806118	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	5215	0.13414694376477804	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	5177	0.1331694588437691	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5133	0.13203763419839035	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4804	0.12357467264544461	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	4769	0.1226743575866206	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4739	0.12190265896477144	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4707	0.12107951376813236	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA	4693	0.12071938774460274	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4641	0.1193817768000642	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	4536	0.11668083162359216	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4514	0.11611491930090279	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4436	0.11410850288409498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08704760454458463	0.0	0.0	0.0	0.0
2	0.14541374364377568	0.0	0.0	0.0	0.0
3	0.18435880075976302	0.0	0.0	0.0	0.0
4	0.23433914816819326	0.0	0.0	0.0	0.0
5	0.27114917243039793	0.0	0.0	0.0	0.0
6	0.3693864069917953	0.0	0.0	0.0	0.0
7	0.4048588203094614	0.0	0.0	0.0	0.0
8	0.4470707349246102	0.0	0.0	0.0	0.0
9	0.4876620824338757	0.0	0.0	0.0	0.0
10	0.5275074546086871	0.0	0.0	0.0	0.0
11	0.5691020103263565	0.0	0.0	0.0	0.0
12	0.6097705276978069	0.0	0.0	0.0	0.0
13	0.6592106860709428	0.0	0.0	0.0	0.0
14	0.6905159268306235	0.0	0.0	0.0	0.0
15	0.7234417346961874	0.0	0.0	0.0	0.0
16	0.7545669124441033	0.0	0.0	0.0	0.0
17	0.7915569997180728	0.0	0.0	0.0	0.0
18	0.8306563965584299	0.0	0.0	0.0	0.0
19	0.8656915139903816	0.0	0.0	0.0	0.0
20	0.9081092149046901	0.0	0.0	0.0	0.0
21	0.9590413239467342	0.0	0.0	0.0	0.0
22	1.003131038541716	0.0	0.0	0.0	0.0
23	1.0452143367198898	0.0	0.0	0.0	0.0
24	1.0849310924577265	0.0	0.0	0.0	2.572328739497182E-5
25	1.128969360477918	0.0	0.0	0.0	2.572328739497182E-5
26	1.1758886366863468	0.0	0.0	0.0	2.572328739497182E-5
27	1.2228593594695651	0.0	0.0	0.0	2.572328739497182E-5
28	1.2719908383939613	0.0	0.0	0.0	2.572328739497182E-5
29	1.3229486707234006	0.0	0.0	0.0	2.572328739497182E-5
30	1.3754499002965381	0.0	0.0	0.0	2.572328739497182E-5
31	1.425584587429338	0.0	0.0	0.0	2.572328739497182E-5
32	1.4753334252512136	0.0	0.0	0.0	2.572328739497182E-5
33	1.5154103070125797	0.0	0.0	0.0	2.572328739497182E-5
34	1.5511142299168006	0.0	0.0	0.0	2.572328739497182E-5
35	1.580927520007573	0.0	0.0	0.0	2.572328739497182E-5
36	1.6031267170294337	0.0	0.0	0.0	2.572328739497182E-5
37	1.621956163402553	0.0	0.0	0.0	2.572328739497182E-5
38	1.6333258564311306	0.0	0.0	0.0	2.572328739497182E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	100	0.0	169.9669	44
TTTACGC	290	0.0	153.2858	44
TACGGTA	55	7.2941475E-10	142.62956	44
CTATCAT	1975	0.0	116.51091	44
ACGACAC	365	0.0	100.29659	44
CTATGCG	105	5.456968E-12	99.614296	44
ACTCGAC	80	0.0	98.71989	43
AACGACA	530	0.0	91.89021	43
CCGGGCT	460	0.0	90.952194	44
TACGCGA	25	7.2930015E-7	87.992485	42
ATGTTCT	750	0.0	83.67602	44
CCTTCGT	110	9.440555E-10	83.200584	44
CTCGACA	175	0.0	82.1818	44
TCCGGGC	685	0.0	79.74453	43
GTACGGC	25	0.0013785246	78.97591	43
CGTGCGC	150	1.8189894E-12	78.446266	44
TATTCGT	50	0.0014154853	78.446266	44
CAATGAA	2800	0.0	76.39039	43
CCGAAAC	35	5.5538276E-5	75.21516	43
TGCGTAT	35	5.5538276E-5	75.21516	43
>>END_MODULE