FastQCFastQC Report
Tue 24 May 2016
ERR840986.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840986.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518367
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC60401.1651976302503824No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA40550.7822643030902816No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG31810.6136578910308719No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT22190.42807508965655605No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA21310.4110987003416498No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA19670.3794608838911428No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG12890.2486655207603879No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA12630.24364976937189287No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG12600.24307102882706655No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG12360.23844110446845576No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT10030.19349225548694263No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG8400.1620473525513777No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC8350.16108278497666711No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT8020.1547166389835773No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC7380.14237017402728183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA7150.13793316318361315No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA7120.1373544226387868No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC7010.1352323739744235No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA6840.13195284422040757No Hit
AGACGCTGTAGTGAGTGCTGAGGGTAAAGCCTCTGCCTTCCAGGTGGAG6690.1290591414962758No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC6670.12867331446639158No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG6570.1267441793169704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.11709850356986459No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT6050.11671267653998035No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG5970.11516936842044342No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC5940.11459062787561708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA5930.11439771436067496No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT5540.10687408727793243No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA5230.10089376831472682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA152.6027219E-5213.9258744
TCCCTAG152.6027219E-5213.9258744
TAAGAGT253.608875E-9213.9258744
CCTGATA152.6027219E-5213.9258744
AGCCCTT203.0564843E-7213.9258644
CGAATTA100.0022123477213.9258644
TACGACA450.0190.1563444
CCGTACC401.8189894E-12187.1851344
AATAGGG351.2551027E-10183.3650244
CGCGGGT301.07302185E-8178.2715644
TTATCCC301.07302185E-8178.2715644
TATCACA259.2914706E-7171.140744
GAGCAAT259.2914706E-7171.140744
CTTTCGA259.2914706E-7171.140744
TAGGAAC259.2914706E-7171.140744
GCCGTCA208.195265E-5160.444444
TCCGATG150.0074405964142.6172544
TAGGTCC150.0074405964142.6172544
GTACTTC150.0074405964142.6172544
TCACTAC150.0074405964142.6172544
Produced by FastQC (version 0.11.5)