##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840986.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518367
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.038598136069616	39.0	37.0	39.0	33.0	39.0
2	37.672664347846215	39.0	37.0	39.0	35.0	39.0
3	38.001977363528155	39.0	38.0	39.0	35.0	40.0
4	38.389195299855125	39.0	38.0	40.0	35.0	41.0
5	38.99312649146261	40.0	38.0	41.0	35.0	41.0
6	39.017043909045135	40.0	38.0	41.0	35.0	41.0
7	39.07337079713793	40.0	38.0	41.0	35.0	41.0
8	38.999818661295954	40.0	38.0	41.0	35.0	41.0
9	39.04243132761152	40.0	38.0	41.0	35.0	41.0
10	39.02367627568884	40.0	38.0	41.0	35.0	41.0
11	38.93011900834737	40.0	38.0	41.0	35.0	41.0
12	38.85992742593568	40.0	38.0	41.0	35.0	41.0
13	38.85443324903013	40.0	38.0	41.0	35.0	41.0
14	38.80424293984764	40.0	38.0	41.0	35.0	41.0
15	38.79536313075485	40.0	38.0	41.0	35.0	41.0
16	38.77022071235245	40.0	38.0	41.0	35.0	41.0
17	38.72784147138996	40.0	38.0	41.0	35.0	41.0
18	38.70568728333401	40.0	38.0	41.0	35.0	41.0
19	38.6399095621442	40.0	38.0	41.0	35.0	41.0
20	38.67082202377852	40.0	38.0	41.0	35.0	41.0
21	38.52936433067691	40.0	38.0	41.0	35.0	41.0
22	38.43477883430079	40.0	37.0	41.0	34.0	41.0
23	38.29978760222005	40.0	37.0	41.0	34.0	41.0
24	38.20954265993013	40.0	37.0	41.0	34.0	41.0
25	38.10972268276933	40.0	37.0	41.0	34.0	41.0
26	37.95846435956459	40.0	36.0	41.0	33.0	41.0
27	37.82680441112086	39.0	36.0	41.0	33.0	41.0
28	37.73174260858564	39.0	36.0	41.0	33.0	41.0
29	37.68889932800408	39.0	36.0	41.0	33.0	41.0
30	37.7141012086407	39.0	36.0	41.0	33.0	41.0
31	37.61172811092614	39.0	36.0	41.0	33.0	41.0
32	37.6182547741091	39.0	36.0	41.0	33.0	41.0
33	37.450729971984195	39.0	35.0	41.0	33.0	41.0
34	37.4031546601244	39.0	35.0	41.0	33.0	41.0
35	37.308740440500095	38.0	35.0	40.0	32.0	41.0
36	37.26160069669966	38.0	35.0	40.0	32.0	41.0
37	37.22896869774302	38.0	35.0	40.0	32.0	41.0
38	37.10144869469866	38.0	35.0	40.0	32.0	41.0
39	36.93444703076496	38.0	35.0	40.0	32.0	41.0
40	36.70297438574794	38.0	35.0	40.0	31.0	41.0
41	36.611624478448284	38.0	34.0	40.0	31.0	41.0
42	36.40684484339307	37.0	34.0	40.0	31.0	41.0
43	36.28865127410643	37.0	34.0	40.0	31.0	41.0
44	36.01792417925533	37.0	34.0	40.0	30.0	41.0
45	35.796951184397436	37.0	34.0	40.0	29.0	41.0
46	35.51963603702529	36.0	33.0	40.0	29.0	41.0
47	35.650600349693214	36.0	34.0	40.0	29.0	41.0
48	36.353611148851925	37.0	34.0	40.0	31.0	41.0
49	37.244854595190766	39.0	35.0	40.0	32.0	41.0
50	36.69781388848328	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	5.0
22	16.0
23	23.0
24	58.0
25	101.0
26	186.0
27	391.0
28	835.0
29	1707.0
30	3299.0
31	6305.0
32	11407.0
33	20413.0
34	31496.0
35	30376.0
36	49364.0
37	80946.0
38	91564.0
39	82239.0
40	107635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.55427525286139	11.966232418344532	41.47949232879407
2	27.024675567696242	27.92596750950581	28.049239245553824	17.000117677244113
3	25.426580009915757	23.188783236587206	23.05972409509093	28.32491265840611
4	20.414493978204632	26.11528125825911	27.57390034473645	25.89632441879981
5	18.187693275227783	29.0714879612321	29.613574938219443	23.12724382532067
6	23.1081453873414	30.512551917849706	25.717493590448466	20.661809104360422
7	26.717557251908396	29.690354517166412	21.6020695761883	21.990018654736897
8	23.427610168085543	26.958120405041214	26.78334076050366	22.83092866636958
9	23.39462195703044	29.589460748851682	27.035285811018063	19.980631483099813
10	26.24742701599446	24.472429765012045	27.346262397104752	21.933880821888742
11	23.68129144023443	24.483425835363747	26.889829020751705	24.945453703650116
12	21.457963180526537	26.80976991205073	26.78989982001169	24.942367087411043
13	23.52271653095201	25.25565863567704	25.588820275982076	25.632804557388877
14	19.2541191858278	29.003389490457533	26.3516003140632	25.39089100965146
15	20.446710535199962	30.711638665269973	27.931562001439136	20.91008879809093
16	21.83645949684297	35.87111062239687	21.87465637280151	20.417773507958646
17	21.716274377034033	34.59826725080879	19.248331780379537	24.43712659177764
18	23.37513769202129	26.62129340795228	24.163382314074777	25.840186585951653
19	20.057218148531835	25.006800201401706	26.65871862985105	28.27726302021541
20	25.310831900950486	23.822697046687	24.291669801511283	26.57480125085123
21	22.177916418290515	30.593189767095513	23.583484288158775	23.645409526455193
22	23.321121907837497	29.375519660780874	21.36883713662328	25.934521294758344
23	24.678268485455284	27.332565537543864	25.445871361409967	22.543294615590884
24	23.14325564706087	29.363559022854464	23.49185036856127	24.001334961523398
25	21.95985580512361	28.509902247663817	25.988431056582336	23.54181089063024
26	24.572978034411456	30.87940384690414	24.995708321953884	19.55190979673052
27	23.057471399767156	27.50432414709757	26.248996267784957	23.189208185350317
28	25.42879790310561	26.900458794483395	23.077303213538094	24.5934400888729
29	24.090536322812827	28.350425172776205	25.96320742698812	21.595831077422847
30	25.545850653979546	27.59188953232253	27.228856797302388	19.633403016395544
31	21.259355539919337	30.481368002481968	23.063851876785687	25.195424580813008
32	21.30845046874554	27.910239001096933	26.906483327828273	23.87482720232925
33	22.132076866830435	26.23606398448861	29.236261163190026	22.395597985490934
34	21.627677413492595	29.40198580857268	24.489298606464615	24.48103817147011
35	20.89624140086256	27.460967754662168	23.466275184856798	28.176515659618474
36	21.608131504148663	28.042161189067492	23.91647122459166	26.43323608219218
37	20.406998579554394	33.29633600567339	22.794060565114947	23.502604849657267
38	25.048289823288766	28.382607228712413	24.509721226434987	22.05938172156383
39	22.911074662145676	31.1623801410193	25.95807614533094	19.968469051504087
40	20.192914624152415	30.49724300695186	26.48660445869004	22.823237910205687
41	22.861071358419622	30.041162922718613	25.09276227602686	22.005003442834912
42	21.565220042377405	33.73685505665201	21.85768074092947	22.840244160041117
43	19.475548131317918	27.22949342066117	28.658414303312917	24.63654414470799
44	23.129297742162542	26.53477899904344	25.103100691248397	25.232822567545615
45	20.76366760087534	26.993031827081815	28.428177909164205	23.815122662878643
46	20.827392105073788	29.87875896514687	26.199417382057177	23.094431547722166
47	22.90135683546999	31.49658371668398	22.174227223164916	23.427832224681115
48	23.119116476776462	29.585605869451292	25.033287397371947	22.261990256400303
49	18.828162484499916	28.503953689798923	22.017908438900072	30.649975386801092
50	20.94015311124554	32.999228185819476	24.542647948434468	21.517970754500514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7666.0
1	7356.5
2	6105.0
3	5005.0
4	4445.0
5	2969.5
6	1462.5
7	1158.0
8	1308.0
9	1323.0
10	1342.5
11	1340.0
12	1298.5
13	1325.0
14	1434.5
15	1361.5
16	1195.5
17	1346.0
18	1696.5
19	1953.5
20	2206.5
21	2603.0
22	2843.5
23	3428.0
24	4394.5
25	4718.5
26	4685.0
27	5194.5
28	6174.0
29	7376.0
30	8399.0
31	9136.0
32	10616.0
33	12736.5
34	17066.5
35	22108.0
36	24235.5
37	25533.5
38	27261.5
39	29264.5
40	30874.5
41	31963.5
42	33298.5
43	33299.5
44	33054.0
45	33651.5
46	35283.5
47	37716.5
48	41905.5
49	45675.0
50	48600.0
51	49367.0
52	44286.0
53	41144.5
54	40187.5
55	37260.0
56	34704.5
57	32873.0
58	28730.5
59	23623.5
60	21168.0
61	20365.5
62	19249.5
63	15324.0
64	10312.0
65	8199.5
66	6870.0
67	4709.5
68	3511.5
69	2802.0
70	2024.0
71	1659.5
72	1508.5
73	1202.5
74	821.0
75	567.0
76	396.0
77	210.5
78	115.0
79	94.0
80	74.0
81	50.0
82	28.0
83	16.5
84	11.5
85	10.5
86	9.5
87	7.0
88	4.0
89	3.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	2676.0
25	3071.0
26	3271.0
27	3458.0
28	3507.0
29	2980.0
30	6247.0
31	2699.0
32	3590.0
33	2632.0
34	2647.0
35	2760.0
36	2218.0
37	2391.0
38	2937.0
39	3926.0
40	4076.0
41	4074.0
42	5438.0
43	10513.0
44	26504.0
45	41549.0
46	65217.0
47	52381.0
48	97122.0
49	64605.0
50	95878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.237403229758067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.36668382970652	9.876014483946701
2	11.174493916154034	3.1819155154552665
3	4.048670767729872	1.7292767479411306
4	2.1367984607463213	1.2168984522548696
5	1.3793664128343406	0.981929791055372
6	0.9037695455407714	0.7720398867983493
7	0.7208476735047831	0.7184099296444411
8	0.6409040405409068	0.7299847405409681
9	0.45662719167502236	0.5851066908194387
>10	5.4483618330126555	18.899158318334308
>50	2.3021056339936585	23.40503928683732
>100	1.382076366494133	30.624248843001194
>500	0.02438958293813176	2.332903136195012
>1k	0.013549768298962088	3.781876546925248
>5k	0.0013549768298962088	1.1651976302503824
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	6040	1.1651976302503824	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4055	0.7822643030902816	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	3181	0.6136578910308719	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2219	0.42807508965655605	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2131	0.4110987003416498	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1967	0.3794608838911428	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1289	0.2486655207603879	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1263	0.24364976937189287	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1260	0.24307102882706655	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	1236	0.23844110446845576	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	1003	0.19349225548694263	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	840	0.1620473525513777	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	835	0.16108278497666711	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	802	0.1547166389835773	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	738	0.14237017402728183	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	715	0.13793316318361315	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	712	0.1373544226387868	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	701	0.1352323739744235	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	684	0.13195284422040757	No Hit
AGACGCTGTAGTGAGTGCTGAGGGTAAAGCCTCTGCCTTCCAGGTGGAG	669	0.1290591414962758	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	667	0.12867331446639158	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	657	0.1267441793169704	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	607	0.11709850356986459	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	605	0.11671267653998035	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	597	0.11516936842044342	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	594	0.11459062787561708	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	593	0.11439771436067496	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	554	0.10687408727793243	No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA	523	0.10089376831472682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07060634646881457	0.0	0.0	0.0	0.0
2	0.13870481724338163	0.0	0.0	0.0	0.0
3	0.17362216344790468	0.0	0.0	0.0	0.0
4	0.2058387204432381	0.0	0.0	0.0	0.0
5	0.22513007193744972	0.0	0.0	0.0	0.0
6	0.2662206506201205	0.0	0.0	0.0	0.0
7	0.29245688865224834	0.0	0.0	0.0	0.0
8	0.32698840782688715	0.0	0.0	0.0	0.0
9	0.35457504046360977	0.0	0.0	0.0	0.0
10	0.38409080824975356	0.0	0.0	0.0	0.0
11	0.40897665167728653	0.0	0.0	0.0	0.0
12	0.4363703707990671	0.0	0.0	0.0	0.0
13	0.4676223602196899	0.0	0.0	0.0	0.0
14	0.5017680523644446	0.0	0.0	0.0	0.0
15	0.5235672795529036	0.0	0.0	0.0	0.0
16	0.5505751716448	0.0	0.0	0.0	0.0
17	0.5791263718562332	0.0	0.0	0.0	0.0
18	0.6049767828584767	0.0	0.0	0.0	0.0
19	0.6310201073756624	0.0	0.0	0.0	0.0
20	0.658413826497443	0.0	0.0	0.0	0.0
21	0.6904374699778343	0.0	0.0	0.0	0.0
22	0.7155162269203094	0.0	0.0	0.0	0.0
23	0.7419453784673793	0.0	0.0	0.0	0.0
24	0.7681816164995071	0.0	0.0	0.0	0.0
25	0.7940320275017507	0.0	0.0	0.0	0.0
26	0.8256698439522577	0.0	0.0	0.0	0.0
27	0.8474690711407169	0.0	0.0	0.0	0.0
28	0.8729336551130763	0.0	0.0	0.0	0.0
29	0.903992731018757	0.0	0.0	0.0	0.0
30	0.9441187421267172	0.0	0.0	0.0	0.0
31	0.9734415963979188	0.0	0.0	0.0	0.0
32	1.0008353155196994	0.0	0.0	0.0	0.0
33	1.028421948156422	0.0	0.0	0.0	0.0
34	1.056394407823029	0.0	0.0	0.0	0.0
35	1.0797369431310249	0.0	0.0	0.0	0.0
36	1.1003786892298313	0.0	0.0	0.0	0.0
37	1.1219850029033485	0.0	0.0	0.0	0.0
38	1.1324023327102226	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	15	2.6027219E-5	213.92587	44
TCCCTAG	15	2.6027219E-5	213.92587	44
TAAGAGT	25	3.608875E-9	213.92587	44
CCTGATA	15	2.6027219E-5	213.92587	44
AGCCCTT	20	3.0564843E-7	213.92586	44
CGAATTA	10	0.0022123477	213.92586	44
TACGACA	45	0.0	190.15634	44
CCGTACC	40	1.8189894E-12	187.18513	44
AATAGGG	35	1.2551027E-10	183.36502	44
CGCGGGT	30	1.07302185E-8	178.27156	44
TTATCCC	30	1.07302185E-8	178.27156	44
TATCACA	25	9.2914706E-7	171.1407	44
GAGCAAT	25	9.2914706E-7	171.1407	44
CTTTCGA	25	9.2914706E-7	171.1407	44
TAGGAAC	25	9.2914706E-7	171.1407	44
GCCGTCA	20	8.195265E-5	160.4444	44
TCCGATG	15	0.0074405964	142.61725	44
TAGGTCC	15	0.0074405964	142.61725	44
GTACTTC	15	0.0074405964	142.61725	44
TCACTAC	15	0.0074405964	142.61725	44
>>END_MODULE