FastQCFastQC Report
Tue 24 May 2016
ERR840985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070414
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC835292.052100842813532No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA569861.400005011775215No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT385190.946316517189652No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT303200.7448873750925581No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC232900.572177670379475No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA195190.4795335314786162No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA158760.390034035849916No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC136510.33537129147059735No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA136310.33487994095932255No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA126600.31102487363693226No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT124500.3058656932685471No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG112070.2753282589928199No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC103940.2553548607095003No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT91750.2254070470473028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA85920.21108417964364312No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG85890.21101047706695192No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC84670.20801323894817578No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG84020.20641634978653278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA83940.20621980958202288No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA83180.20435267763917872No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC83100.20415613743466882No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT80370.19744920295576815No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC75910.18649208655434066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA75480.1854356829550999No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG73830.181382041237083No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT73070.17951490929423886No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT70230.17253773203413708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA69210.17003184442663571No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA68780.16897544082739496No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA64030.15730586618461906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA61820.1518764430350328No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61400.15084460696135576No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC59540.14627504720650036No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT55140.13546533595845534No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT54740.1344826349359058No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC54240.13325425865771884No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA53640.13178020712389452No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA52340.1285864288006085No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC48340.11875941857511299No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG47270.11613069333979295No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT46920.1152708299450621No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC46740.1148286144849148No Hit
AAAAAAAAAAAAAAAAAAAAAAAA46700.11473034438265985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46460.11414072376913012No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACA41510.10197979861507946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC401.5478327E-7114.1353444
CTATCAT72700.0103.2398844
ACATTGG19450.0101.87092644
AACGACA2250.0100.9027443
GAACACA41450.091.8589844
CCTATCG209.251741E-487.3196843
ACTTCTA39700.081.6484544
CCTTAGC135250.077.3018643
TTAGAGT8500.075.19504544
GACATTG17500.073.1822143
GTCATCG506.419394E-573.04661644
TGAACAC35550.070.0849943
TGGCCGA551.0291504E-466.4060144
TGCGAAT551.0291504E-466.4060144
TAATTCC7300.066.29230544
GATAGTT1259.094947E-1265.7419644
CCCTATC75250.065.7016942
CCTATCA73400.065.588943
CAGGCAC15350.063.0532744
AATCGCA7450.062.50633244
Produced by FastQC (version 0.11.5)