##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840985.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4070414
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.059544803059346	39.0	37.0	39.0	33.0	39.0
2	37.66751318170584	39.0	37.0	39.0	35.0	39.0
3	37.974604548824765	39.0	38.0	39.0	35.0	39.0
4	38.42113136403324	39.0	38.0	40.0	35.0	41.0
5	39.24539420314494	40.0	39.0	41.0	35.0	41.0
6	39.13930204642575	40.0	38.0	41.0	35.0	41.0
7	39.24643021569796	40.0	38.0	41.0	35.0	41.0
8	39.19880041686177	40.0	38.0	41.0	35.0	41.0
9	39.253410095385874	40.0	39.0	41.0	35.0	41.0
10	39.23241493371436	40.0	39.0	41.0	35.0	41.0
11	39.12719934630728	40.0	39.0	41.0	35.0	41.0
12	39.12564004545975	40.0	39.0	41.0	35.0	41.0
13	39.059802025051994	40.0	38.0	41.0	35.0	41.0
14	38.997024135628465	40.0	38.0	41.0	35.0	41.0
15	38.95578017371206	40.0	38.0	41.0	35.0	41.0
16	38.93777733665421	40.0	38.0	41.0	35.0	41.0
17	38.88689430608287	40.0	38.0	41.0	35.0	41.0
18	38.83008460564454	40.0	38.0	41.0	35.0	41.0
19	38.79448331299961	40.0	38.0	41.0	35.0	41.0
20	38.71407650425731	40.0	38.0	41.0	35.0	41.0
21	38.63606601195849	40.0	38.0	41.0	35.0	41.0
22	38.546245418770674	40.0	38.0	41.0	35.0	41.0
23	38.465405730227936	40.0	37.0	41.0	35.0	41.0
24	38.28988722031715	40.0	37.0	41.0	34.0	41.0
25	38.216612394747806	40.0	37.0	41.0	34.0	41.0
26	38.10342123823273	40.0	37.0	41.0	34.0	41.0
27	38.05150757164006	40.0	37.0	41.0	34.0	41.0
28	37.859307728398676	39.0	36.0	41.0	33.0	41.0
29	37.88354314855019	39.0	36.0	41.0	33.0	41.0
30	37.842482014710754	39.0	36.0	41.0	33.0	41.0
31	37.82528825579578	39.0	36.0	41.0	33.0	41.0
32	37.82435555495489	39.0	36.0	41.0	33.0	41.0
33	37.66904595270986	39.0	36.0	41.0	33.0	41.0
34	37.62861773686627	39.0	35.0	41.0	33.0	41.0
35	37.485074462830816	39.0	35.0	41.0	33.0	41.0
36	37.44575327842839	39.0	35.0	41.0	33.0	41.0
37	37.3518052363276	38.0	35.0	41.0	33.0	41.0
38	37.122643645252694	38.0	35.0	41.0	32.0	41.0
39	37.15044867651292	38.0	35.0	41.0	32.0	41.0
40	36.94210689352854	38.0	35.0	40.0	31.0	41.0
41	36.8833733901825	38.0	35.0	40.0	31.0	41.0
42	36.745987135242075	38.0	35.0	40.0	31.0	41.0
43	36.64290491129901	38.0	34.0	40.0	31.0	41.0
44	36.42917006071981	38.0	34.0	40.0	30.0	41.0
45	36.188104792766396	38.0	34.0	40.0	29.0	41.0
46	35.80099786448456	37.0	34.0	40.0	28.0	41.0
47	35.865479343120406	37.0	34.0	40.0	29.0	41.0
48	36.8056657343743	38.0	35.0	40.0	31.0	41.0
49	37.820981009590355	39.0	36.0	40.0	34.0	41.0
50	37.29288107540772	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	15.0
21	71.0
22	150.0
23	290.0
24	591.0
25	1049.0
26	1746.0
27	3261.0
28	6150.0
29	12161.0
30	23748.0
31	45660.0
32	83201.0
33	146248.0
34	209955.0
35	218206.0
36	375046.0
37	667236.0
38	654024.0
39	575570.0
40	1046030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.367217683508365	16.266134108225845	35.366648208265794
2	22.40297915641996	30.069914264249288	31.342585791027645	16.184520788303107
3	24.316617425156263	27.308843768717384	21.28817854891419	27.086360257212167
4	19.370019855474162	33.350072007417424	24.48660013453177	22.793308002576644
5	17.717558951005966	31.432404664488672	24.477215339766424	26.372821044738938
6	16.600105050739312	32.407956537099174	25.534994720438757	25.45694369172276
7	21.429589226059072	29.619886330972722	20.38517457929341	28.5653498636748
8	18.635205165862736	28.47803687782127	28.62138838948569	24.2653695668303
9	18.978413497988164	34.86984370631587	25.573246357741496	20.57849643795447
10	26.85522897670851	25.306320192491476	23.07352519915665	24.76492563164337
11	17.8367114499901	28.85158610401792	24.268809020409225	29.042893425582754
12	16.666437369761404	29.289256571935923	25.094032204095214	28.95027385420746
13	17.75870955632523	31.103543767292468	22.291467157886153	28.846279518496154
14	15.300237273161896	30.4742465999773	25.19353068262835	29.031985444232454
15	16.83678859202037	38.26067815214865	24.187171132960923	20.715362122870058
16	16.841210746621844	40.93075053299246	19.4119320540859	22.816106666299792
17	19.02543574191716	40.8471226759735	18.13945706751205	21.987984514597287
18	18.504948145323795	32.003034580757635	20.947820049754153	28.54419722416442
19	18.967923164572447	28.871019016738835	23.785836035351686	28.37522178333703
20	18.405105721432758	29.475404713132374	26.341006099133896	25.778483466300973
21	17.411644122686294	38.80492254596216	22.273434594122367	21.509998737229186
22	19.778626940650263	32.53123638037801	20.097316882263083	27.59281979670864
23	22.72854800519063	28.93003021314294	25.532537967882384	22.808883813784053
24	17.04752882630612	33.35518205273468	24.17314307586403	25.42414604509517
25	17.065023045185757	30.890627632385687	22.83681814880634	29.207531173622215
26	18.605336842270127	37.77589558311007	25.16781268124652	18.450954893373286
27	20.69659188614864	32.92925228490466	20.34848862477316	26.025667204173537
28	19.989834373439212	28.92002199609519	21.24979189474162	29.84035173572398
29	18.55428333189276	32.81333733304183	25.843447003015037	22.788932332050376
30	20.15686013517924	31.443187475762574	28.156624640198224	20.24332774885996
31	18.70784957108674	35.813760090082766	21.294423159120814	24.183967179709683
32	16.796402096489892	33.31145807721957	23.943685033738145	25.9484547925524
33	16.32630627357846	29.79016707173506	29.45630626834389	24.42722038634259
34	17.77125544155354	36.30117757257707	23.73365889467889	22.193908091190504
35	17.202363848947773	32.25909648852172	21.76099596270701	28.777543699823493
36	15.408167099189429	32.06495222619763	25.38320166681367	27.143679007799264
37	14.628189851464708	39.69868217694458	20.38166673995652	25.291461231634187
38	21.448518477242807	31.0264247414179	22.069935829058824	25.45512095228047
39	16.567054324622347	34.52788475640559	26.310885022569625	22.59417589640244
40	15.50487237924226	32.55473500934378	26.200771221533508	25.73962138988045
41	16.249184052778705	36.60717103416431	21.982850021287987	25.160794891768994
42	15.297367205181233	37.2127923087609	23.583908123725955	23.905932362331914
43	17.56081484394228	29.135338664916503	21.780950575827955	31.52289591531326
44	17.74149258427601	30.85916823679143	20.93590289209346	30.4634362868391
45	15.682134008386178	29.297100016985123	31.25168868990304	23.76907728472566
46	16.351346028935936	32.82515542400228	26.624917640131933	24.198580906929845
47	16.849272245633536	35.88365463255981	24.39823176522787	22.868841356578788
48	19.548594857415726	29.843925319382663	23.5596788648772	27.047800958324412
49	16.48657673540173	31.222402148584067	21.36591636176928	30.925104754244927
50	17.08223442237652	33.77413118754149	25.60316755476613	23.54046683531586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120485.0
1	107151.5
2	69860.0
3	43683.5
4	38271.5
5	28087.0
6	16058.5
7	10557.0
8	9289.0
9	8595.5
10	8817.0
11	9120.0
12	9340.0
13	10483.5
14	13757.0
15	16747.0
16	17957.0
17	18950.0
18	20291.5
19	22020.5
20	23852.5
21	26147.5
22	28703.5
23	33730.5
24	38940.5
25	41595.5
26	46120.0
27	54158.5
28	60546.5
29	64495.5
30	73532.5
31	97834.5
32	136234.5
33	157908.5
34	192774.0
35	243423.5
36	241669.0
37	222104.0
38	217717.0
39	218982.5
40	234044.0
41	260888.0
42	284679.5
43	295751.0
44	298794.5
45	309627.5
46	326270.5
47	317530.5
48	333296.0
49	381647.0
50	430726.0
51	450728.0
52	383430.5
53	278596.0
54	212386.0
55	185823.0
56	168000.5
57	147544.5
58	129108.0
59	113607.0
60	98456.5
61	83316.0
62	70572.5
63	60480.0
64	49844.5
65	40531.0
66	31339.5
67	21944.5
68	14548.5
69	10460.5
70	8656.0
71	7216.5
72	5735.5
73	4172.5
74	2974.5
75	2093.5
76	1608.5
77	1118.5
78	714.5
79	565.0
80	490.5
81	371.5
82	254.5
83	210.0
84	170.5
85	137.5
86	99.5
87	91.0
88	96.5
89	87.0
90	76.5
91	41.0
92	9.0
93	4.5
94	2.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22280.0
25	25643.0
26	26263.0
27	31888.0
28	30720.0
29	26955.0
30	34670.0
31	24421.0
32	26820.0
33	20139.0
34	24570.0
35	23256.0
36	23305.0
37	23601.0
38	23943.0
39	30849.0
40	38725.0
41	34685.0
42	42771.0
43	64379.0
44	161753.0
45	312780.0
46	478459.0
47	391954.0
48	733040.0
49	500753.0
50	891792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.213033564390695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91568634839552	9.027347583079298
2	11.707229773188455	2.8596158033196937
3	3.925538350224355	1.4382819488877878
4	1.8352714284197456	0.8965692622023044
5	1.0712294077729727	0.654148035614684
6	0.7204366871423264	0.5279230464652603
7	0.5255563806939262	0.44930464021762306
8	0.3816542335214609	0.3728924771191525
9	0.3033960834738231	0.3334847895693434
>10	2.85472515994877	7.936731380420009
>50	1.2542442096814586	11.37449390049442
>100	1.3735271171717724	29.291747329293415
>500	0.08059034345407455	6.86468198287297
>1k	0.0398587619249394	9.472524901229626
>5k	0.0072734967016312765	6.346972237795693
>10k+	0.0037822182848482643	12.153280681418718
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	83529	2.052100842813532	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	56986	1.400005011775215	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	38519	0.946316517189652	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	30320	0.7448873750925581	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	23290	0.572177670379475	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	19519	0.4795335314786162	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	15876	0.390034035849916	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	13651	0.33537129147059735	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13631	0.33487994095932255	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	12660	0.31102487363693226	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	12450	0.3058656932685471	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	11207	0.2753282589928199	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	10394	0.2553548607095003	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	9175	0.2254070470473028	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	8592	0.21108417964364312	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	8589	0.21101047706695192	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	8467	0.20801323894817578	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	8402	0.20641634978653278	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	8394	0.20621980958202288	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	8318	0.20435267763917872	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	8310	0.20415613743466882	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	8037	0.19744920295576815	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	7591	0.18649208655434066	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	7548	0.1854356829550999	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	7383	0.181382041237083	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	7307	0.17951490929423886	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT	7023	0.17253773203413708	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6921	0.17003184442663571	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	6878	0.16897544082739496	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	6403	0.15730586618461906	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6182	0.1518764430350328	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6140	0.15084460696135576	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	5954	0.14627504720650036	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	5514	0.13546533595845534	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	5474	0.1344826349359058	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	5424	0.13325425865771884	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5364	0.13178020712389452	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	5234	0.1285864288006085	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC	4834	0.11875941857511299	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	4727	0.11613069333979295	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	4692	0.1152708299450621	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	4674	0.1148286144849148	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	4670	0.11473034438265985	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4646	0.11414072376913012	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACA	4151	0.10197979861507946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.09139119509710805	0.0	0.0	0.0	0.0
2	0.18833465097162108	0.0	0.0	0.0	0.0
3	0.23874721342841293	0.0	0.0	0.0	0.0
4	0.2896265588709158	0.0	0.0	0.0	0.0
5	0.32883632967064286	0.0	0.0	0.0	0.0
6	0.38961638791533243	0.0	0.0	0.0	0.0
7	0.4302510751977563	0.0	0.0	0.0	0.0
8	0.4853068999860948	0.0	0.0	0.0	0.0
9	0.5359405701729603	0.0	0.0	0.0	4.913505112747745E-5
10	0.5825697336929364	0.0	0.0	0.0	4.913505112747745E-5
11	0.6280687910369805	0.0	0.0	0.0	4.913505112747745E-5
12	0.6797834323486506	0.0	0.0	0.0	4.913505112747745E-5
13	0.7380821705114026	0.0	0.0	0.0	4.913505112747745E-5
14	0.7838514706366477	0.0	0.0	0.0	4.913505112747745E-5
15	0.8379737294535642	0.0	0.0	0.0	4.913505112747745E-5
16	0.8865437274930756	0.0	0.0	0.0	4.913505112747745E-5
17	0.9513282924046547	0.0	0.0	0.0	7.370257669121618E-5
18	1.0044432826734577	0.0	0.0	0.0	7.370257669121618E-5
19	1.0487385312648787	0.0	0.0	0.0	7.370257669121618E-5
20	1.0987580133126507	0.0	0.0	0.0	7.370257669121618E-5
21	1.1381151892657602	0.0	0.0	0.0	7.370257669121618E-5
22	1.1793886322128413	0.0	0.0	0.0	7.370257669121618E-5
23	1.222381801949384	0.0	0.0	0.0	7.370257669121618E-5
24	1.265178431481417	0.0	0.0	0.0	7.370257669121618E-5
25	1.3045110399089626	0.0	0.0	0.0	7.370257669121618E-5
26	1.3485851807703098	0.0	0.0	0.0	7.370257669121618E-5
27	1.3834464995452551	0.0	0.0	0.0	7.370257669121618E-5
28	1.422951080651747	0.0	0.0	0.0	7.370257669121618E-5
29	1.4647650091612303	0.0	0.0	0.0	7.370257669121618E-5
30	1.5054488314947816	0.0	0.0	0.0	7.370257669121618E-5
31	1.5443883595133074	0.0	0.0	0.0	7.370257669121618E-5
32	1.5809694050777146	0.0	0.0	0.0	7.370257669121618E-5
33	1.6168134248752093	0.0	0.0	0.0	7.370257669121618E-5
34	1.6519204189057919	0.0	0.0	0.0	7.370257669121618E-5
35	1.6822613129770092	0.0	0.0	0.0	7.370257669121618E-5
36	1.7079835122422438	0.0	0.0	0.0	7.370257669121618E-5
37	1.7319614171924527	0.0	0.0	0.0	7.370257669121618E-5
38	1.7478566062321916	0.0	0.0	0.0	7.370257669121618E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATAC	40	1.5478327E-7	114.13534	44
CTATCAT	7270	0.0	103.23988	44
ACATTGG	1945	0.0	101.870926	44
AACGACA	225	0.0	100.90274	43
GAACACA	4145	0.0	91.85898	44
CCTATCG	20	9.251741E-4	87.31968	43
ACTTCTA	3970	0.0	81.64845	44
CCTTAGC	13525	0.0	77.30186	43
TTAGAGT	850	0.0	75.195045	44
GACATTG	1750	0.0	73.18221	43
GTCATCG	50	6.419394E-5	73.046616	44
TGAACAC	3555	0.0	70.08499	43
TGGCCGA	55	1.0291504E-4	66.40601	44
TGCGAAT	55	1.0291504E-4	66.40601	44
TAATTCC	730	0.0	66.292305	44
GATAGTT	125	9.094947E-12	65.74196	44
CCCTATC	7525	0.0	65.70169	42
CCTATCA	7340	0.0	65.5889	43
CAGGCAC	1535	0.0	63.05327	44
AATCGCA	745	0.0	62.506332	44
>>END_MODULE