FastQCFastQC Report
Tue 24 May 2016
ERR840984.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840984.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8871982
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG339140.38225956725340515No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238220.2685082093268449No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA159470.1797456306832002No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154670.1743353401753971No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC147500.16625371872936623No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134410.15149940565704484No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA130100.14664141563858No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT125240.14116349649942933No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA123710.1394389664000671No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG108370.122148579652213No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98790.11135054151372263No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA98050.11051645506043632No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA97500.10989652593975055No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG93700.10561337928773976No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG92580.10435097816925236No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT89450.10082301790062242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCG456.5949826E-6115.5390144
ACGGTCG602.7525515E-586.6542744
AGGTACG500.001449151577.9888444
CCTTAGC28050.071.32902543
CGGACGG1901.8189894E-1161.57013744
CTATCAT25250.060.74378244
TATCGCA650.004100948659.99141344
TCGTGCG29500.058.40909643
TCGCGTT1003.8198777E-1156.80445543
TTCGCGT1000.054.83958442
TACGCCC2403.6379788E-1254.15891644
ACCTTAG26500.052.53138442
TAACGGT2301.1641532E-1050.86228644
GTTACGC204.263081E-449.8138939
TCGACTA355.009303E-448.2106242
GCGCATT400.001253256147.3370443
ACTCGTG32700.045.4975941
ACTTCTA23850.043.59963244
GGTAAGT2451.1430529E-842.44290544
CGCGTAG505.6997138E-542.18429642
Produced by FastQC (version 0.11.5)