##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840984.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8871982
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.48743144429283	39.0	37.0	39.0	31.0	39.0
2	37.34471947756431	39.0	37.0	39.0	34.0	39.0
3	37.83030116607541	39.0	37.0	39.0	35.0	39.0
4	38.349682968247684	39.0	38.0	40.0	35.0	41.0
5	38.939724291595724	40.0	38.0	41.0	35.0	41.0
6	38.94675890911411	40.0	38.0	41.0	35.0	41.0
7	38.95617360359839	40.0	38.0	41.0	35.0	41.0
8	38.91071859703953	40.0	38.0	41.0	35.0	41.0
9	38.918661241648145	40.0	38.0	41.0	35.0	41.0
10	38.899266590035914	40.0	38.0	41.0	35.0	41.0
11	38.848765473149065	40.0	38.0	41.0	35.0	41.0
12	38.77624469932423	40.0	38.0	41.0	35.0	41.0
13	38.71967627977604	40.0	38.0	41.0	35.0	41.0
14	38.720223282689254	40.0	38.0	41.0	35.0	41.0
15	38.71069012538574	40.0	38.0	41.0	35.0	41.0
16	38.654459398136744	40.0	38.0	41.0	35.0	41.0
17	38.633677457866796	40.0	38.0	41.0	35.0	41.0
18	38.60195433218868	40.0	38.0	41.0	35.0	41.0
19	38.512691527101836	40.0	37.0	41.0	35.0	41.0
20	38.434499416252194	40.0	37.0	41.0	35.0	41.0
21	38.34400892607762	40.0	37.0	41.0	34.0	41.0
22	38.231100897183964	40.0	36.0	41.0	34.0	41.0
23	38.14188159984995	40.0	36.0	41.0	34.0	41.0
24	38.0163825850864	40.0	36.0	41.0	34.0	41.0
25	37.90219862588572	39.0	36.0	41.0	34.0	41.0
26	37.81959395682599	39.0	35.0	41.0	34.0	41.0
27	37.714799372423094	39.0	35.0	41.0	33.0	41.0
28	37.62141468446467	39.0	35.0	41.0	33.0	41.0
29	37.590007185571245	39.0	35.0	41.0	33.0	41.0
30	37.563835468413224	39.0	35.0	41.0	33.0	41.0
31	37.45782912049274	39.0	35.0	41.0	33.0	41.0
32	37.41808407555229	38.0	35.0	41.0	33.0	41.0
33	37.31147726471975	38.0	35.0	40.0	33.0	41.0
34	37.19923524006187	38.0	35.0	40.0	33.0	41.0
35	37.173208322704056	38.0	35.0	40.0	33.0	41.0
36	37.091379452781744	38.0	35.0	40.0	32.0	41.0
37	37.008398571089046	38.0	35.0	40.0	32.0	41.0
38	36.89913496525321	38.0	35.0	40.0	32.0	41.0
39	36.77991912594488	38.0	35.0	40.0	32.0	41.0
40	36.5729116624522	38.0	35.0	40.0	31.0	41.0
41	36.470038912552056	37.0	34.0	40.0	31.0	41.0
42	36.347996228445	37.0	34.0	40.0	31.0	41.0
43	36.242606701563055	37.0	34.0	40.0	31.0	41.0
44	36.07192971292907	37.0	34.0	40.0	30.0	41.0
45	35.84468855009672	37.0	34.0	40.0	29.0	41.0
46	35.53443011212588	36.0	33.0	40.0	29.0	41.0
47	35.71439565927348	37.0	34.0	40.0	29.0	41.0
48	36.547284624403474	37.0	35.0	40.0	31.0	41.0
49	37.128252749034075	38.0	35.0	40.0	33.0	41.0
50	36.880322575911	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	53.0
21	152.0
22	409.0
23	944.0
24	1755.0
25	3287.0
26	5152.0
27	9258.0
28	17864.0
29	34201.0
30	64599.0
31	125415.0
32	231999.0
33	419834.0
34	646235.0
35	505448.0
36	807185.0
37	1370226.0
38	1360932.0
39	1337310.0
40	1929713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.73799338186214	25.216417256031402	26.04558936210646
2	27.049953437687318	25.41765752004456	29.573910316770252	17.958478725497866
3	27.2151589126308	21.943315484634663	24.92354019654233	25.917985406192212
4	22.588639156391434	26.648746582218042	27.394374785701775	23.368239475688746
5	22.38331863162031	30.299396459550977	25.88258181768178	21.43470309114694
6	23.58422278133567	35.33738008034732	23.54366814540426	17.534728992912747
7	26.98113003385264	32.78301286003511	22.099458722977573	18.136398383134683
8	25.167251241041743	30.24979085845756	25.08985027246449	19.493107628036217
9	23.92422572543542	30.55347722752368	26.02525568694797	19.497041360092933
10	24.719053758224486	29.262424112222053	25.390842767715267	20.6276793618382
11	23.382509116903076	29.81221107076187	28.051815253908313	18.753464558426742
12	22.912275971705082	28.876174455719138	28.196326367659445	20.015223204916328
13	19.50892145633298	30.044402705055084	29.107216403279445	21.33945943533249
14	17.836386503038444	32.49436259000525	28.0544978562851	21.614753050671204
15	18.53709802386885	35.771803865246795	25.841001480841598	19.850096630042756
16	19.145721891681024	38.99211021843823	22.71857630008717	19.143591589793576
17	20.46590040421633	37.94967122340871	21.844882011708318	19.739546360666647
18	22.385775805225936	32.480476177701895	23.418848234813822	21.71489978225835
19	20.56092990269818	32.195590568150386	26.170769958730755	21.07270957042068
20	22.545976761449698	30.65328581595409	25.24683886869924	21.553898553896975
21	23.466492605598162	30.8331892467771	24.445360687160996	21.25495746046374
22	20.73724901605977	35.31439761712772	23.903091778139316	20.045261588673196
23	22.266106942056467	32.673927877671524	24.18467485619335	20.875290324078655
24	24.22227637522258	31.744823197341926	24.487267895719356	19.545632531716137
25	20.828427548374336	33.090694917344436	25.674223868676066	20.406653665605166
26	21.878275328438686	33.52542715715058	25.68113464288262	18.915162871528114
27	22.8761792781677	32.626078819233804	23.502601874959144	20.99514002763935
28	21.444558624506847	33.39869047579395	23.895112640284218	21.261638259414983
29	22.247583439070258	33.3296593743775	23.668528421323305	20.754228765228945
30	24.009602352240066	32.9155347786717	23.9702862734595	19.10457659562874
31	21.190816346827763	34.47556833273075	23.819766945260568	20.51384837518092
32	21.85833829191254	34.73452982085614	24.807955991335618	18.59917589589571
33	22.119552288992754	32.78000027429795	24.08261523233273	21.017832204376568
34	19.723352580128605	34.64278114408467	25.490626846366826	20.1432394294199
35	23.146818354284658	33.09953710953704	24.476950034559586	19.276694501618717
36	21.40368003669142	34.46613517502319	24.36459523456497	19.76558955372042
37	20.58108319771742	34.9786975671382	23.83533294991978	20.60488628522461
38	21.451937004980685	34.77636792478657	23.210465831462102	20.56122923877064
39	22.571126717955163	34.41299315083761	23.58350988402157	19.43237024718566
40	20.198242342593282	34.723072234346404	24.845575404472463	20.233110018587848
41	21.239507146071446	34.754550920501345	24.234263630223825	19.77167830320338
42	21.956733486910483	34.99555781126219	22.570282314517094	20.47742638731023
43	20.341597273209786	34.39932331814349	24.563438216706153	20.695641191940574
44	21.08276814021718	34.02490722102412	24.012183775243592	20.880140863515102
45	22.104349598486785	32.286866883169516	24.463360447639065	21.145423070704638
46	21.150730943978036	33.722042161176475	25.257504725268532	19.869722169576953
47	21.792780758529585	33.17341163081722	25.263409078049776	19.77039853260342
48	22.026614581056915	34.15295084516114	23.995086641765504	19.82534793201643
49	20.48387987438077	35.2215882674628	23.03043042222812	21.26410143592831
50	20.323749981655144	36.72735144337311	24.16809756527099	18.78080100970076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	217190.0
1	191711.5
2	161183.5
3	152659.5
4	135512.5
5	110995.5
6	86765.5
7	71839.0
8	67379.0
9	62441.0
10	58388.0
11	54582.5
12	51280.5
13	50074.5
14	52986.0
15	57557.5
16	60619.0
17	62302.5
18	64614.5
19	70472.0
20	77017.0
21	86533.0
22	100404.5
23	116695.5
24	126752.5
25	130614.0
26	138129.0
27	152087.0
28	173678.5
29	198585.5
30	223158.0
31	247482.5
32	284194.0
33	327626.5
34	375200.5
35	407379.5
36	421329.5
37	446618.0
38	473219.0
39	506360.5
40	545089.0
41	579150.5
42	606122.5
43	608388.0
44	602375.5
45	614106.5
46	619752.0
47	602206.0
48	594370.0
49	605357.5
50	626655.5
51	624870.5
52	607212.0
53	588006.5
54	530191.0
55	478855.0
56	441651.5
57	402383.0
58	353471.5
59	299788.5
60	269836.5
61	243174.5
62	218416.0
63	186840.0
64	146701.5
65	120081.0
66	99315.0
67	78775.0
68	61234.5
69	53005.5
70	48453.0
71	41176.5
72	34953.5
73	23787.0
74	11657.5
75	5115.5
76	3199.5
77	2403.5
78	1776.5
79	1369.0
80	1087.5
81	735.0
82	449.0
83	313.5
84	227.5
85	151.5
86	81.0
87	49.0
88	40.0
89	36.0
90	39.5
91	38.0
92	31.0
93	31.0
94	15.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	41421.0
25	40412.0
26	40305.0
27	43323.0
28	41935.0
29	39616.0
30	78480.0
31	42710.0
32	45824.0
33	46166.0
34	46528.0
35	49421.0
36	48006.0
37	49090.0
38	53951.0
39	79942.0
40	98226.0
41	89618.0
42	121419.0
43	200597.0
44	480705.0
45	847023.0
46	962803.0
47	1097508.0
48	1380058.0
49	1444115.0
50	1362780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.79261633788614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.17256137014023	9.1048327139286
2	12.671696225505668	3.242082963262679
3	4.397507865830085	1.6876689291120228
4	1.9660274214683675	1.0060253805043358
5	1.1309345070180803	0.7233805625779351
6	0.7016555029406113	0.5385605790291468
7	0.534423059273515	0.47856684115838216
8	0.42119279489558414	0.431052626350494
9	0.34314768143401564	0.39507809722384823
>10	3.3684787399222578	9.52249435425117
>50	1.4081134799810453	13.448547723765067
>100	1.7831922227786303	38.16731880643551
>500	0.05895699180691477	5.205559310822775
>1k	0.035945716943501606	8.672366342168896
>5k	0.0046624151684039735	4.181001680228764
>10k+	0.0015040048930335399	3.19546308918039
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	33914	0.38225956725340515	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23822	0.2685082093268449	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	15947	0.1797456306832002	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15467	0.1743353401753971	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	14750	0.16625371872936623	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13441	0.15149940565704484	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	13010	0.14664141563858	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	12524	0.14116349649942933	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	12371	0.1394389664000671	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	10837	0.122148579652213	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9879	0.11135054151372263	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	9805	0.11051645506043632	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	9750	0.10989652593975055	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	9370	0.10561337928773976	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	9258	0.10435097816925236	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	8945	0.10082301790062242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03678997545306111	0.0	0.0	0.0	0.0
2	0.06999563344470267	0.0	0.0	0.0	0.0
3	0.09591994212792587	0.0	0.0	0.0	0.0
4	0.1265782550054768	0.0	0.0	0.0	0.0
5	0.1632667875115166	0.0	0.0	0.0	0.0
6	0.2166257776447247	0.0	0.0	0.0	0.0
7	0.25138689415735965	0.0	0.0	0.0	0.0
8	0.2851673955154553	0.0	0.0	0.0	0.0
9	0.31695285224879854	0.0	0.0	0.0	0.0
10	0.3443649908216676	0.0	0.0	0.0	0.0
11	0.3744484603327644	0.0	0.0	0.0	0.0
12	0.4039570864774072	0.0	0.0	0.0	0.0
13	0.43798555948377715	0.0	0.0	0.0	0.0
14	0.47030077382934277	0.0	0.0	0.0	0.0
15	0.5040474608717647	0.0	0.0	0.0	0.0
16	0.5358216461665499	0.0	0.0	0.0	0.0
17	0.5699402906813833	1.1271438557923134E-5	0.0	0.0	0.0
18	0.6000575745081539	1.1271438557923134E-5	0.0	0.0	0.0
19	0.6338718901819232	1.1271438557923134E-5	0.0	0.0	0.0
20	0.6662321902817205	1.1271438557923134E-5	0.0	0.0	0.0
21	0.6954364875852994	1.1271438557923134E-5	0.0	0.0	0.0
22	0.7268274439691154	1.1271438557923134E-5	0.0	0.0	0.0
23	0.7592102869460285	1.1271438557923134E-5	0.0	0.0	0.0
24	0.7890345133702932	1.1271438557923134E-5	0.0	0.0	0.0
25	0.8205043698240145	1.1271438557923134E-5	0.0	0.0	0.0
26	0.8515909973667666	1.1271438557923134E-5	0.0	0.0	0.0
27	0.8812236093355464	1.1271438557923134E-5	0.0	0.0	0.0
28	0.9117241220732865	1.1271438557923134E-5	0.0	0.0	0.0
29	0.944794522802233	1.1271438557923134E-5	0.0	0.0	0.0
30	0.9788117243700449	1.1271438557923134E-5	0.0	0.0	0.0
31	1.0124907827811191	1.1271438557923134E-5	0.0	0.0	0.0
32	1.0459894981752669	1.1271438557923134E-5	0.0	0.0	0.0
33	1.0805026430396274	1.1271438557923134E-5	0.0	0.0	0.0
34	1.112829128823751	1.1271438557923134E-5	0.0	0.0	0.0
35	1.140692124938937	1.1271438557923134E-5	0.0	0.0	0.0
36	1.1645763032431762	1.1271438557923134E-5	0.0	0.0	0.0
37	1.1847296353847427	1.1271438557923134E-5	0.0	0.0	0.0
38	1.1984131617940614	1.1271438557923134E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGCG	45	6.5949826E-6	115.53901	44
ACGGTCG	60	2.7525515E-5	86.65427	44
AGGTACG	50	0.0014491515	77.98884	44
CCTTAGC	2805	0.0	71.329025	43
CGGACGG	190	1.8189894E-11	61.570137	44
CTATCAT	2525	0.0	60.743782	44
TATCGCA	65	0.0041009486	59.991413	44
TCGTGCG	2950	0.0	58.409096	43
TCGCGTT	100	3.8198777E-11	56.804455	43
TTCGCGT	100	0.0	54.839584	42
TACGCCC	240	3.6379788E-12	54.158916	44
ACCTTAG	2650	0.0	52.531384	42
TAACGGT	230	1.1641532E-10	50.862286	44
GTTACGC	20	4.263081E-4	49.81389	39
TCGACTA	35	5.009303E-4	48.21062	42
GCGCATT	40	0.0012532561	47.33704	43
ACTCGTG	3270	0.0	45.49759	41
ACTTCTA	2385	0.0	43.599632	44
GGTAAGT	245	1.1430529E-8	42.442905	44
CGCGTAG	50	5.6997138E-5	42.184296	42
>>END_MODULE