FastQCFastQC Report
Tue 24 May 2016
ERR840983.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840983.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386279
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC66271.7155993465862756No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA43481.1256112809653127No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT25490.6598857302623233No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT19750.5112884728395797No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA15160.39246244294926724No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC11500.2977122753243122No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG8440.21849492206410392No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA7490.19390129931992162No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGCCG6760.1750030418428131No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA6340.1641300717874904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.1641300717874904No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC6320.1636123113086655No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.16335343106925307No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA6190.1602468681963037No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC6160.15947022747806636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA5940.15377486221099257No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC5920.15325710173216767No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT5920.15325710173216767No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.15092717957745566No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.15092717957745566No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT5780.14963277838039346No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.14367853287390722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.1214148322844369No Hit
CGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGACTTGTGTT4670.12089707180561199No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG4640.12012043108737466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4550.11779050893266266No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTAT4460.11546058677795067No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT4460.11546058677795067No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA4420.11442506582030086No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT4400.11390730534147599No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGC4250.1100241017502893No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG4170.10795305983498973No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGCCGC4050.1048464969620404No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTCCTTACAGCCG4030.10432873648321551No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC3950.10225769456791595No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA3930.10173993408909106No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC3920.10148105384967859No Hit
ATCAACATCGTGCGACAGTCCGGGCAGTTGAGAAGGCCACCCCGCTTCG3890.10070441313144125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAC104.401863E-4366.5429444
ATAGCGA104.401863E-4366.5429444
GTGATCA202.0689185E-8366.5429444
TCTAGGT104.401863E-4366.5429444
GTAATAG301.8189894E-12366.5429444
CGTTAAT202.0689185E-8366.5429444
GCTAAGT153.0214087E-6366.5429444
CTATCAT1700.0344.981644
GCGTCCT256.299342E-8293.2343444
TCCGCTC209.528432E-6274.907244
ACCACGC209.528432E-6274.907244
CTCTGTA407.2759576E-12274.907244
CCCCTTG150.0014826041244.3619544
CCCTAAC150.0014826041244.3619544
TTATCAG150.0014826041244.3619544
GTATGAA301.5639489E-7244.3619544
GCCTAAA301.5639489E-7244.3619544
TGGATTC301.5639489E-7244.3619544
TAGATCT150.0014826041244.3619544
CTGAGAC252.3212328E-5219.9257544
Produced by FastQC (version 0.11.5)