##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840983.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 386279 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.976340934920096 39.0 37.0 39.0 33.0 39.0 2 37.63465267332679 39.0 37.0 39.0 35.0 39.0 3 38.04700747387251 39.0 38.0 39.0 35.0 40.0 4 38.60832714178094 39.0 38.0 41.0 35.0 41.0 5 38.9842704366533 40.0 38.0 41.0 35.0 41.0 6 39.005734197302985 40.0 38.0 41.0 35.0 41.0 7 39.01865232124967 40.0 38.0 41.0 35.0 41.0 8 38.997354243953204 40.0 38.0 41.0 35.0 41.0 9 38.9447912001429 40.0 38.0 41.0 35.0 41.0 10 38.94627199511234 40.0 38.0 41.0 35.0 41.0 11 38.77205853799973 40.0 38.0 41.0 35.0 41.0 12 38.778393337458155 40.0 38.0 41.0 35.0 41.0 13 38.79062801757279 40.0 38.0 41.0 35.0 41.0 14 38.6132355111202 40.0 38.0 41.0 35.0 41.0 15 38.65058157445784 40.0 38.0 41.0 35.0 41.0 16 38.665832209361625 40.0 38.0 41.0 35.0 41.0 17 38.56494140245781 40.0 38.0 41.0 35.0 41.0 18 38.519888474392864 40.0 38.0 41.0 35.0 41.0 19 38.464640842499854 40.0 37.0 41.0 35.0 41.0 20 38.39152788528499 40.0 37.0 41.0 34.0 41.0 21 38.29900149891659 40.0 37.0 41.0 34.0 41.0 22 38.15481556077343 40.0 36.0 41.0 34.0 41.0 23 38.02357622340329 40.0 36.0 41.0 34.0 41.0 24 37.86845259514496 39.0 36.0 41.0 33.0 41.0 25 37.763596102745794 39.0 36.0 41.0 33.0 41.0 26 37.60633752390145 39.0 35.0 41.0 33.0 41.0 27 37.511972402061396 39.0 35.0 41.0 33.0 41.0 28 37.32326839999787 38.0 35.0 40.0 33.0 41.0 29 37.30437771190446 38.0 35.0 40.0 33.0 41.0 30 37.284326174122334 38.0 35.0 40.0 33.0 41.0 31 37.212518361975285 38.0 35.0 40.0 33.0 41.0 32 37.10179081278317 38.0 35.0 40.0 32.0 41.0 33 37.054761772391174 38.0 35.0 40.0 32.0 41.0 34 36.943121182089484 38.0 35.0 40.0 32.0 41.0 35 36.78843558160943 38.0 35.0 40.0 32.0 41.0 36 36.69944686962333 38.0 35.0 40.0 31.0 41.0 37 36.72359070310354 38.0 35.0 40.0 32.0 41.0 38 36.45212521010338 37.0 34.0 40.0 31.0 41.0 39 36.307329153641376 37.0 34.0 40.0 31.0 41.0 40 36.10343265479096 37.0 34.0 40.0 31.0 41.0 41 36.046574058794405 37.0 34.0 40.0 31.0 41.0 42 35.88444308179564 36.0 34.0 40.0 30.0 41.0 43 35.627560442616165 36.0 34.0 40.0 30.0 41.0 44 35.32420790150393 36.0 33.0 39.0 29.0 41.0 45 35.14366419612508 36.0 33.0 39.0 28.0 41.0 46 34.878698350702834 35.0 33.0 39.0 28.0 41.0 47 35.282560129844086 36.0 34.0 39.0 29.0 41.0 48 35.95181825203035 36.0 34.0 39.0 31.0 41.0 49 36.652148826295594 38.0 35.0 40.0 32.0 41.0 50 37.067222339949616 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 3.0 22 11.0 23 17.0 24 38.0 25 104.0 26 182.0 27 376.0 28 765.0 29 1414.0 30 2877.0 31 5330.0 32 9912.0 33 17216.0 34 27503.0 35 26392.0 36 42668.0 37 66261.0 38 62158.0 39 55260.0 40 67789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 43.51129623924676 14.322031485014715 42.16667227573852 2 24.995663755989842 23.543345612886025 30.671354124868294 20.78963650625584 3 24.45745173825137 26.062768102847944 23.968426966001257 25.511353192899435 4 24.930684815897315 26.479047527823152 28.742178580766755 19.848089075512775 5 20.3831945303783 27.93654327571522 27.979258515218277 23.701003678688203 6 24.17579003777063 26.76536907261332 23.413387732700976 25.645453156915078 7 27.475218689082244 25.176621043339143 23.38439314588678 23.963767121691834 8 26.489661617639065 22.578240080356426 29.753623676151175 21.178474625853333 9 23.61583207992151 27.00172673119688 26.498204665539678 22.884236523341936 10 30.075152933501432 19.172670530885707 29.425622412815606 21.326554122797255 11 22.011551236282585 21.576891314309087 28.60730197603287 27.804255473375463 12 24.445543247238394 24.11857750486048 27.700703377610488 23.735175870290647 13 25.980444186714784 21.59630733226502 26.814297437862272 25.608951043157923 14 20.446102428555527 24.70623564832673 24.580160971732866 30.26750095138488 15 20.067878398773946 31.090222352237628 25.85307510892386 22.988824140064565 16 22.36492276308057 31.760981052555277 20.08444673409634 25.78964945026781 17 21.720569847182993 32.92490660895363 22.554940858809307 22.79958268505407 18 21.23206283541171 24.112105498875167 23.546969936237797 31.10886172947533 19 23.054579720875328 22.046500068603265 25.292081630117092 29.60683858040432 20 24.250606426960825 22.78586203236521 23.250552062110547 29.712979478563422 21 24.601389151364685 30.039427460462516 21.47126817662881 23.887915211543987 22 24.030817103699658 24.66326152858426 25.06633806134944 26.239583306366644 23 25.353954007336664 23.568198115869617 29.49474343673873 21.58310444005499 24 23.08020886457716 24.289956223351517 26.593990354122283 26.035844557949044 25 23.778200385557234 26.751159276819674 24.693638305632263 24.77700203199083 26 24.795083579670194 32.289581983617175 23.728075582356073 19.187258854356557 27 24.99636799716836 26.75848369825056 24.628677237776 23.61647106680508 28 23.22631850893033 24.88587393664339 23.97446346101603 27.913344093410252 29 21.625692743910296 27.961614469218542 28.35443785711217 22.058254929758988 30 26.099127953411433 26.293788461694657 26.10589639945744 21.50118718543647 31 25.06866969195568 30.973171038045464 22.241062018424778 21.717097251574078 32 22.781464619183577 27.678226935499872 25.084331903916752 24.4559765413998 33 23.794129093234 21.303997331406016 32.15044198587869 22.751431589481292 34 25.780939422953097 29.452572522802477 24.464302711661322 20.302185342583105 35 23.300521265721272 20.37539038453936 26.212662860240922 30.111425489498444 36 21.038008161872394 24.355084107099692 28.77159554651841 25.835312184509508 37 22.701238212945164 29.871029021331136 24.267314382495652 23.160418383228052 38 24.30464414818908 26.28278188390781 24.7412562455389 24.67131772236421 39 26.56491958636129 29.12638251721078 23.229189019898968 21.07950887652896 40 21.065306659262614 25.72226037906797 25.419473630372202 27.79295933129722 41 23.159727952096937 29.551616021122612 23.152655649590987 24.13600037718947 42 21.310504335505303 32.47982568678988 21.826657181043437 24.383012796661376 43 23.821599451112434 23.12098195260102 23.551991682056272 29.50542691423027 44 25.255824826618873 24.35190524429206 22.4680121561599 27.924257772929167 45 21.907502109618846 22.247752281607852 29.532291571352566 26.31245403742074 46 21.999265260349194 26.48311065565846 28.37023144299 23.14739264100234 47 22.182630555335315 28.213571132078112 26.770081758864983 22.83371655372159 48 24.21504149808996 26.318759988355584 24.96717581288134 24.499022700673116 49 18.072451364112823 26.604287074345834 23.933760950888093 31.389500610653254 50 18.848155211791575 31.633349815168 28.07327352781898 21.445221445221446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7109.0 1 6782.5 2 6543.0 3 6599.0 4 6262.5 5 4213.5 6 1851.5 7 1329.0 8 1386.5 9 1276.0 10 1115.5 11 963.0 12 910.0 13 923.0 14 934.0 15 882.5 16 782.5 17 848.0 18 1037.0 19 1173.0 20 1304.0 21 1435.5 22 1600.0 23 1847.0 24 2130.0 25 2522.5 26 2941.5 27 3160.0 28 3185.0 29 3450.0 30 4367.0 31 5861.5 32 7333.0 33 7989.5 34 10051.5 35 12539.0 36 12165.5 37 11661.5 38 12154.0 39 13051.0 40 14052.5 41 14334.0 42 16107.5 43 18884.5 44 21039.0 45 21453.5 46 21274.0 47 23594.0 48 28579.0 49 33927.5 50 41229.5 51 45873.0 52 42607.5 53 37937.5 54 34191.0 55 31492.5 56 28462.0 57 25282.0 58 25423.5 59 26723.0 60 24749.5 61 20733.0 62 17487.0 63 15279.0 64 13066.0 65 10920.0 66 8171.5 67 5599.5 68 4723.5 69 4066.0 70 3107.5 71 2248.0 72 1678.5 73 1071.0 74 565.5 75 326.0 76 236.5 77 186.0 78 133.5 79 98.0 80 78.0 81 49.5 82 21.5 83 13.0 84 8.5 85 7.0 86 8.0 87 9.0 88 8.5 89 6.5 90 3.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 2419.0 25 2607.0 26 2674.0 27 3117.0 28 3030.0 29 3071.0 30 3115.0 31 3394.0 32 3112.0 33 2542.0 34 3060.0 35 2503.0 36 2099.0 37 2088.0 38 2606.0 39 2687.0 40 2803.0 41 3405.0 42 5096.0 43 10026.0 44 25748.0 45 36482.0 46 44184.0 47 46090.0 48 64334.0 49 61516.0 50 42471.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.535035557200883 #Duplication Level Percentage of deduplicated Percentage of total 1 67.92838972514966 9.194131702732998 2 11.472180249794388 3.1055273519916953 3 4.370445460283457 1.774624041172313 4 2.279899776217891 1.2343409815185395 5 1.4976187288411147 1.0135161372997237 6 1.0557925138190234 0.8574113529340193 7 0.8052330585467551 0.7629200655484767 8 0.6082282960044374 0.6585913290652611 9 0.5202455865195188 0.6337388260816663 >10 5.63280607463229 18.327426549204073 >50 2.2703364382304 22.138920314073506 >100 1.516745404816097 32.97849481851201 >500 0.030602681559971694 2.617796980938648 >1k 0.009563337987491155 2.986960202340795 >5k 0.0019126675974982309 1.7155993465862756 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 6627 1.7155993465862756 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 4348 1.1256112809653127 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 2549 0.6598857302623233 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 1975 0.5112884728395797 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 1516 0.39246244294926724 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 1150 0.2977122753243122 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 844 0.21849492206410392 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 749 0.19390129931992162 No Hit AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGCCG 676 0.1750030418428131 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 634 0.1641300717874904 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 634 0.1641300717874904 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 632 0.1636123113086655 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.16335343106925307 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 619 0.1602468681963037 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 616 0.15947022747806636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 594 0.15377486221099257 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC 592 0.15325710173216767 No Hit TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT 592 0.15325710173216767 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.15092717957745566 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.15092717957745566 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 578 0.14963277838039346 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 555 0.14367853287390722 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 469 0.1214148322844369 No Hit CGTCTTGTTCTTGCCTGGTGTCGGTGGTTAGTTTCTGCGACTTGTGTT 467 0.12089707180561199 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 464 0.12012043108737466 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 455 0.11779050893266266 No Hit CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTAT 446 0.11546058677795067 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 446 0.11546058677795067 No Hit CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA 442 0.11442506582030086 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.11390730534147599 No Hit AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGC 425 0.1100241017502893 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 417 0.10795305983498973 No Hit AAGCTCTGTTTGGTGCTTTGGATCCATTTCCGTCGGTCCTTACAGCCGC 405 0.1048464969620404 No Hit AAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTCCTTACAGCCG 403 0.10432873648321551 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 395 0.10225769456791595 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 393 0.10173993408909106 No Hit CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC 392 0.10148105384967859 No Hit ATCAACATCGTGCGACAGTCCGGGCAGTTGAGAAGGCCACCCCGCTTCG 389 0.10070441313144125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.08077063469668297 0.0 0.0 0.0 0.0 2 0.14807949694391878 0.0 0.0 0.0 0.0 3 0.18380496998283624 0.0 0.0 0.0 0.0 4 0.21564723943056702 0.0 0.0 0.0 0.0 5 0.24878391007536005 0.0 0.0 0.0 0.0 6 0.28813370646605174 0.0 0.0 0.0 0.0 7 0.31557501184377096 0.0 0.0 0.0 0.0 8 0.3562192094315249 0.0 0.0 0.0 0.0 9 0.386508197442781 0.0 0.0 0.0 0.0 10 0.412655101623438 0.0 0.0 0.0 0.0 11 0.44268520939528166 0.0 0.0 0.0 0.0 12 0.47349195788536264 0.0 0.0 0.0 0.0 13 0.5032631854177939 0.0 0.0 0.0 0.0 14 0.5332932931896376 0.0 0.0 0.0 0.0 15 0.5646178021585434 0.0 0.0 0.0 0.0 16 0.5918002272968502 0.0 0.0 0.0 0.0 17 0.6303733829693046 0.0 0.0 0.0 0.0 18 0.6676521374446968 0.0 0.0 0.0 0.0 19 0.6925046404282915 0.0 0.0 0.0 0.0 20 0.7202048260454231 0.0 0.0 0.0 0.0 21 0.7416918859166561 0.0 0.0 0.0 0.0 22 0.763178945787889 0.0 0.0 0.0 0.0 23 0.7919146523626706 0.0 0.0 0.0 0.0 24 0.8126250715156661 0.0 0.0 0.0 0.0 25 0.8322999697110119 0.0 0.0 0.0 0.0 26 0.8563758319763695 0.0 0.0 0.0 0.0 27 0.8781217720870148 0.0 0.0 0.0 0.0 28 0.9065985984223838 0.0 0.0 0.0 0.0 29 0.9399941493065893 0.0 0.0 0.0 0.0 30 0.9715775385149076 0.0 0.0 0.0 0.0 31 0.9912524367102534 0.0 0.0 0.0 0.0 32 1.0127394965814864 0.0 0.0 0.0 0.0 33 1.0329321552556572 0.0 0.0 0.0 0.0 34 1.060632340872789 0.0 0.0 0.0 0.0 35 1.074094113322236 0.0 0.0 0.0 0.0 36 1.096098933672294 0.0 0.0 0.0 0.0 37 1.1108551073188033 0.0 0.0 0.0 0.0 38 1.1196570354588264 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAAC 10 4.401863E-4 366.54294 44 ATAGCGA 10 4.401863E-4 366.54294 44 GTGATCA 20 2.0689185E-8 366.54294 44 TCTAGGT 10 4.401863E-4 366.54294 44 GTAATAG 30 1.8189894E-12 366.54294 44 CGTTAAT 20 2.0689185E-8 366.54294 44 GCTAAGT 15 3.0214087E-6 366.54294 44 CTATCAT 170 0.0 344.9816 44 GCGTCCT 25 6.299342E-8 293.23434 44 TCCGCTC 20 9.528432E-6 274.9072 44 ACCACGC 20 9.528432E-6 274.9072 44 CTCTGTA 40 7.2759576E-12 274.9072 44 CCCCTTG 15 0.0014826041 244.36195 44 CCCTAAC 15 0.0014826041 244.36195 44 TTATCAG 15 0.0014826041 244.36195 44 GTATGAA 30 1.5639489E-7 244.36195 44 GCCTAAA 30 1.5639489E-7 244.36195 44 TGGATTC 30 1.5639489E-7 244.36195 44 TAGATCT 15 0.0014826041 244.36195 44 CTGAGAC 25 2.3212328E-5 219.92575 44 >>END_MODULE