FastQCFastQC Report
Tue 24 May 2016
ERR840982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865081
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT113521.3122470612578474No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC107061.2375719730291153No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA68690.7940296920172794No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC61670.7128812215272327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA42220.48804678405837143No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT41210.4763715767656439No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA40370.4666615033736725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA39610.4578761988761746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA38440.4443514537945002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA32630.37719011283336473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30550.35314612157705466No Hit
AAAAAAAAAAAAAAAAAAAAAAAA23520.27188205497519885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21280.2459885259299418No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG20120.23257937696007655No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA19350.22367847635076946No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC17210.1989409084236043No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16920.19558862118113796No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG14310.16541803599894114No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA12880.14888779201022795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12650.1462290814386167No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC12470.14414835142605142No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG12430.1436859669788147No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC12000.13871533417101983No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC11470.13258874024513312No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA11330.13097039467980456No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT10940.1264621463192464No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10790.12472820464210865No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG10590.12241628240592499No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.12172270573506991No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT10520.12160710962326071No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC10450.12079793684059642No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA10320.11929518738707705No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC10120.11698326515089338No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA10090.11663647681546584No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA9730.11247501679033524No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA9590.11085667122500667No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC9550.11039428677776994No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT9400.10866034510063219No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA9340.1079667684297771No Hit
CCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTTT9110.10530805785816588No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.10496126952273833No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC8770.10137779005665365No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG8680.100337425050371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATA100.0013638264251.4827944
CGTTCGG201.3665522E-7251.4827944
AGCATAG100.0013638264251.4827944
GTTCCGA100.0013638264251.4827944
CCAACGC100.0013638264251.4827944
CGATCCT151.3663041E-5251.4827944
TCGGTAC100.0013638264251.4827944
GACCGCA950.0225.0109144
CTATCAT14650.0210.2842444
TCAATAC304.089088E-9209.56944
GAGCATT254.1566273E-7201.1862344
ACGAACA451.8189894E-12195.5977244
ATGGATC401.03682396E-10188.6120944
TCGAAGT204.3045075E-5188.6120944
CGACCCA150.00458923167.655244
CGTGCGC402.2821041E-8157.1767444
GAATACA251.0475743E-4150.8896844
TGGCCTT352.2208005E-6143.7044544
CATTGTT454.6105924E-8139.7126644
TGATATG404.3155287E-6125.74139444
Produced by FastQC (version 0.11.5)