##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840982.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865081
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54310752403532	39.0	37.0	39.0	31.0	39.0
2	37.37443083364448	39.0	37.0	39.0	35.0	39.0
3	37.793693307331914	39.0	37.0	39.0	35.0	39.0
4	38.38642046236133	39.0	38.0	40.0	35.0	41.0
5	38.930252774017696	40.0	38.0	41.0	35.0	41.0
6	38.841997454573615	40.0	38.0	41.0	35.0	41.0
7	38.842378921742586	40.0	38.0	41.0	35.0	41.0
8	38.81902388331266	40.0	38.0	41.0	35.0	41.0
9	38.83187817094584	40.0	38.0	41.0	35.0	41.0
10	38.73588022393279	40.0	38.0	41.0	35.0	41.0
11	38.6475023726102	40.0	38.0	41.0	35.0	41.0
12	38.59212605524801	40.0	38.0	41.0	35.0	41.0
13	38.405037216168196	40.0	37.0	41.0	34.0	41.0
14	38.35475637541456	40.0	37.0	41.0	34.0	41.0
15	38.29395628848628	40.0	37.0	41.0	34.0	41.0
16	38.19682318765526	40.0	37.0	41.0	34.0	41.0
17	38.234440474360206	40.0	37.0	41.0	34.0	41.0
18	38.204950750276566	40.0	37.0	41.0	34.0	41.0
19	38.18545662198106	40.0	36.0	41.0	34.0	41.0
20	38.018806331430234	39.0	36.0	41.0	34.0	41.0
21	37.94615070727481	39.0	36.0	41.0	34.0	41.0
22	37.88495296972191	39.0	36.0	41.0	34.0	41.0
23	37.79588847749517	39.0	35.0	41.0	33.0	41.0
24	37.50691438142787	39.0	35.0	40.0	33.0	41.0
25	37.44580307037799	39.0	35.0	40.0	33.0	41.0
26	37.29335123025633	38.0	35.0	40.0	33.0	41.0
27	37.12930620112045	38.0	35.0	40.0	32.0	41.0
28	37.02819887692576	38.0	35.0	40.0	32.0	41.0
29	36.991796636890406	38.0	35.0	40.0	32.0	41.0
30	36.94917122828663	38.0	35.0	40.0	32.0	41.0
31	36.795709592040474	38.0	35.0	40.0	32.0	41.0
32	36.68302579338729	38.0	35.0	40.0	31.0	41.0
33	36.59749877528526	38.0	35.0	40.0	32.0	41.0
34	36.51272639942876	37.0	35.0	40.0	31.0	41.0
35	36.32103088859573	37.0	34.0	40.0	31.0	41.0
36	36.29566138917928	37.0	34.0	40.0	31.0	41.0
37	36.18463562710548	37.0	34.0	40.0	31.0	41.0
38	36.0126022355867	36.0	34.0	40.0	31.0	41.0
39	35.88609077482043	36.0	34.0	39.0	31.0	41.0
40	35.69293546025693	36.0	34.0	39.0	30.0	41.0
41	35.589949323751966	36.0	34.0	39.0	30.0	41.0
42	35.38146273733332	35.0	34.0	39.0	30.0	41.0
43	35.237653005255815	35.0	33.0	39.0	30.0	41.0
44	34.98453133265456	35.0	33.0	39.0	29.0	41.0
45	34.72023722915378	35.0	33.0	39.0	28.0	41.0
46	34.43040707307515	35.0	32.0	38.0	28.0	40.0
47	34.52905303869875	35.0	33.0	38.0	29.0	40.0
48	35.33713574874847	35.0	34.0	39.0	31.0	40.0
49	35.90948079845308	36.0	34.0	39.0	31.0	41.0
50	36.140624173299166	37.0	34.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	29.0
22	75.0
23	113.0
24	213.0
25	351.0
26	573.0
27	1058.0
28	2111.0
29	4015.0
30	7928.0
31	14951.0
32	27128.0
33	46568.0
34	67361.0
35	70066.0
36	111946.0
37	149857.0
38	138331.0
39	118136.0
40	104267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.95456841613676	20.729735134629014	37.31569644923423
2	19.17323348911836	22.769890911949286	33.90792307309951	24.148952525832843
3	21.374761438524253	26.208644046048867	27.042901184975744	25.373693330451136
4	20.629628901802256	27.559615804762792	31.64027414773877	20.170481145696183
5	16.14773645473661	27.90374542961873	29.771316212007893	26.17720190363677
6	19.460721019187798	29.245700691611535	27.177223866898014	24.116354422302653
7	22.057125286533864	25.713314706946516	22.982356565454566	29.247203441065057
8	22.996574887207093	27.254904454033785	26.631147834711435	23.117372824047692
9	22.328544957061823	30.488590085783873	25.153367141342837	22.029497815811467
10	27.704573328971506	23.720322143244392	24.87778601078974	23.69731851699436
11	24.59099205739116	25.61263049356072	22.410618196446343	27.385759252601776
12	22.615916891019452	29.535846932252586	23.71731664433735	24.130919532390607
13	25.376814425469984	24.130919532390607	24.080288435418186	26.41197760672122
14	19.990497999609286	28.80504831339493	20.151638979471286	31.052814707524494
15	23.760087205706746	32.108207208342336	21.828822965710724	22.302882620240187
16	18.667847288288613	33.486112861107806	21.639476534567283	26.206563316036302
17	20.354047771249167	35.72220404794464	20.523974055608665	23.399774125197524
18	20.15233255614214	30.264911609433103	19.676538959935545	29.90621687448921
19	24.038442642943263	27.164277102375383	21.069703299459817	27.727576955221533
20	21.418225576564506	26.967532520076155	26.112699273247248	25.501542630112095
21	21.276389147374637	33.68655651898493	20.819900101840176	24.217154231800258
22	27.19040182364426	27.65336425144004	20.449992544050787	24.706241380864913
23	27.230976058889283	27.1732935990965	22.71706348885249	22.87866685316173
24	23.169853458809058	28.675465072056838	24.48059777061339	23.674083698520718
25	24.175611439980223	27.383251465916565	21.60938279897934	26.831754295123872
26	24.733579003562493	32.18005027322662	23.183782465179366	19.90258825803152
27	24.272405127305994	31.271223132274905	20.586035760418792	23.87033598000031
28	23.54698644965496	27.216549397869834	20.378795932963488	28.857668219511723
29	22.473934554249666	31.66973078966244	21.63744121657515	24.218893439512744
30	25.513855804498814	32.24894986739405	22.214129192014163	20.023065136092974
31	26.09918843999855	28.869189655021238	22.536541494277493	22.495080410702723
32	20.56097107492895	28.8450082149782	24.67440480933535	25.9196159007575
33	22.00990998346635	24.924220775244432	27.54472760302913	25.521141638260087
34	25.53152414991453	28.06447511548731	24.10213996663486	22.301860767963298
35	20.08347779255095	25.472896257351884	24.909084553630727	29.534541396466434
36	22.031836987947724	25.0794490835004	27.0384979365398	25.850215992012078
37	19.741281601897427	36.325653027170546	21.512509576332555	22.420555794599473
38	24.762264310342097	28.084623873362514	21.460233062817135	25.692878753478254
39	21.879839885777034	28.47371688604159	25.432955825012332	24.213487403169047
40	20.189551474393525	27.263476281262317	26.828813754749515	25.718158489594643
41	21.879697108106168	31.501543057191377	21.500607871014786	25.11815196368767
42	19.62982437402609	31.74590785729739	21.762366801679306	26.861900966997215
43	22.129819275017184	24.132582569030276	24.10272788496921	29.63487027098333
44	23.133606739208336	24.55206561741	22.169196903422502	30.145130739959157
45	22.032665235895387	24.458004437816015	31.05622446133886	22.45310586494974
46	19.72767835658254	29.271998514984222	25.467161213587897	25.533161914845344
47	20.318114251900777	29.910141467709693	26.070565077789954	23.701179202599572
48	23.411043624532883	24.690518639390003	22.674483022592895	29.223954713484225
49	19.08175677424364	30.42262998524807	21.663530186007478	28.832083054500817
50	16.823503624256443	28.72497222285209	30.41352047950538	24.03800367338609
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45492.0
1	42644.5
2	28413.0
3	16021.0
4	13707.5
5	9276.5
6	4370.5
7	2454.0
8	2053.0
9	1620.0
10	1371.5
11	1271.5
12	1251.0
13	1284.0
14	1410.5
15	1570.5
16	1606.0
17	1619.5
18	1748.0
19	1783.0
20	1799.5
21	2199.5
22	2805.5
23	3201.5
24	3368.5
25	3668.5
26	4400.0
27	5278.0
28	5838.5
29	6460.0
30	7812.0
31	11378.0
32	16661.0
33	18674.0
34	19516.0
35	21891.5
36	23488.5
37	25854.0
38	27748.0
39	29414.0
40	32795.5
41	37223.5
42	40612.5
43	40964.5
44	41310.5
45	44165.0
46	49060.5
47	50750.5
48	54703.5
49	68356.0
50	84451.0
51	95070.5
52	92978.0
53	80767.5
54	72471.5
55	68763.0
56	64129.0
57	62341.0
58	61611.5
59	57664.0
60	52724.0
61	47895.0
62	42111.5
63	35326.0
64	28214.5
65	23079.0
66	17811.0
67	12801.5
68	9818.0
69	7788.5
70	6411.5
71	4773.5
72	3349.0
73	2276.5
74	1460.0
75	1018.5
76	781.5
77	541.5
78	380.5
79	324.0
80	280.5
81	203.5
82	101.0
83	41.0
84	30.5
85	22.0
86	13.5
87	8.5
88	4.0
89	2.5
90	3.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	7597.0
25	8921.0
26	10150.0
27	10685.0
28	10746.0
29	9703.0
30	8940.0
31	7710.0
32	6773.0
33	5199.0
34	6236.0
35	5263.0
36	4862.0
37	4732.0
38	5351.0
39	6347.0
40	8045.0
41	7519.0
42	10147.0
43	16927.0
44	43444.0
45	77972.0
46	90039.0
47	94657.0
48	126643.0
49	138170.0
50	132303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.367600220742833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8327807002434	9.468642949244026
2	10.578065952583298	2.8280671352556954
3	4.209650236072519	1.6881876427491938
4	2.135038331694814	1.1416135549623205
5	1.3359639750107655	0.8929316163629192
6	0.9935346356688477	0.7968704289049525
7	0.686268594340754	0.6421634949238837
8	0.6004828678235996	0.6421611933176833
9	0.43591878221543523	0.5244469208432099
>10	4.752214404769459	15.27694264045227
>50	1.979141047268154	18.914839257338095
>100	1.3707805749765278	30.859637066588924
>500	0.060398377824636286	5.461759441844801
>1k	0.026260164271580993	6.7553248087191555
>5k	0.0017506776181053995	1.5253657279578554
>10k+	0.0017506776181053995	2.5810461205350093
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11352	1.3122470612578474	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10706	1.2375719730291153	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6869	0.7940296920172794	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6167	0.7128812215272327	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4222	0.48804678405837143	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4121	0.4763715767656439	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4037	0.4666615033736725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3961	0.4578761988761746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3844	0.4443514537945002	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3263	0.37719011283336473	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3055	0.35314612157705466	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2352	0.27188205497519885	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2128	0.2459885259299418	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2012	0.23257937696007655	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1935	0.22367847635076946	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1721	0.1989409084236043	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1692	0.19558862118113796	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1431	0.16541803599894114	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1288	0.14888779201022795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1265	0.1462290814386167	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1247	0.14414835142605142	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1243	0.1436859669788147	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1200	0.13871533417101983	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	1147	0.13258874024513312	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1133	0.13097039467980456	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	1094	0.1264621463192464	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1079	0.12472820464210865	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1059	0.12241628240592499	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.12172270573506991	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	1052	0.12160710962326071	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1045	0.12079793684059642	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1032	0.11929518738707705	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	1012	0.11698326515089338	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	1009	0.11663647681546584	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	973	0.11247501679033524	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	959	0.11085667122500667	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	955	0.11039428677776994	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	940	0.10866034510063219	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	934	0.1079667684297771	No Hit
CCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTTT	911	0.10530805785816588	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	0.10496126952273833	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	877	0.10137779005665365	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	868	0.100337425050371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06924207097370073	0.0	0.0	0.0	0.0
2	0.12969883744990354	0.0	0.0	0.0	0.0
3	0.18668772057183083	0.0	0.0	0.0	0.0
4	0.23350414585455004	0.0	0.0	0.0	0.0
5	0.2680673832854958	0.0	0.0	0.0	0.0
6	0.3156929813508793	0.0	0.0	0.0	0.0
7	0.35776996604942196	0.0	0.0	0.0	0.0
8	0.4084010630218442	0.0	0.0	0.0	0.0
9	0.44724135658972974	0.0	0.0	0.0	0.0
10	0.4910522829654102	0.0	0.0	0.0	0.0
11	0.5326668832167161	0.0	0.0	0.0	0.0
12	0.5857254985371312	0.0	0.0	0.0	0.0
13	0.6251437726640626	0.0	0.0	0.0	0.0
14	0.6602849906540543	0.0	0.0	0.0	0.0
15	0.6934610747432899	0.0	0.0	0.0	0.0
16	0.7229380832546316	0.0	0.0	0.0	0.0
17	0.7553049945612029	0.0	0.0	0.0	1.1559611180918319E-4
18	0.798884728713265	0.0	0.0	0.0	1.1559611180918319E-4
19	0.821657162739674	0.0	0.0	0.0	1.1559611180918319E-4
20	0.8436204239834189	0.0	0.0	0.0	2.3119222361836638E-4
21	0.8673176269043015	0.0	0.0	0.0	2.3119222361836638E-4
22	0.8854662164583432	0.0	0.0	0.0	2.3119222361836638E-4
23	0.9043083826832401	0.0	0.0	0.0	2.3119222361836638E-4
24	0.9243065100262288	0.0	0.0	0.0	2.3119222361836638E-4
25	0.9480037129471113	0.0	0.0	0.0	2.3119222361836638E-4
26	0.9726256847624674	0.0	0.0	0.0	2.3119222361836638E-4
27	0.9910054665401274	0.0	0.0	0.0	2.3119222361836638E-4
28	1.0166678033617662	0.0	0.0	0.0	2.3119222361836638E-4
29	1.0414053712889313	0.0	0.0	0.0	2.3119222361836638E-4
30	1.0698420147939904	0.0	0.0	0.0	2.3119222361836638E-4
31	1.0900713343605974	0.0	0.0	0.0	2.3119222361836638E-4
32	1.1182767856420381	0.0	0.0	0.0	2.3119222361836638E-4
33	1.1410492196684472	0.0	0.0	0.0	2.3119222361836638E-4
34	1.1637060575830471	0.0	0.0	0.0	2.3119222361836638E-4
35	1.183704184926036	0.0	0.0	0.0	2.3119222361836638E-4
36	1.2025463511509327	0.0	0.0	0.0	2.3119222361836638E-4
37	1.2174582495743174	0.0	0.0	0.0	2.3119222361836638E-4
38	1.2271683229662886	0.0	0.0	0.0	2.3119222361836638E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATATA	10	0.0013638264	251.48279	44
CGTTCGG	20	1.3665522E-7	251.48279	44
AGCATAG	10	0.0013638264	251.48279	44
GTTCCGA	10	0.0013638264	251.48279	44
CCAACGC	10	0.0013638264	251.48279	44
CGATCCT	15	1.3663041E-5	251.48279	44
TCGGTAC	10	0.0013638264	251.48279	44
GACCGCA	95	0.0	225.01091	44
CTATCAT	1465	0.0	210.28424	44
TCAATAC	30	4.089088E-9	209.569	44
GAGCATT	25	4.1566273E-7	201.18623	44
ACGAACA	45	1.8189894E-12	195.59772	44
ATGGATC	40	1.03682396E-10	188.61209	44
TCGAAGT	20	4.3045075E-5	188.61209	44
CGACCCA	15	0.00458923	167.6552	44
CGTGCGC	40	2.2821041E-8	157.17674	44
GAATACA	25	1.0475743E-4	150.88968	44
TGGCCTT	35	2.2208005E-6	143.70445	44
CATTGTT	45	4.6105924E-8	139.71266	44
TGATATG	40	4.3155287E-6	125.741394	44
>>END_MODULE