FastQCFastQC Report
Tue 24 May 2016
ERR840981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638043
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC235871.4399499891028502No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA153230.9354455285972346No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT86650.5289848923379912No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT86170.5260545663331183No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC48740.29755018641146785No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA37530.2291148645059989No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA34700.2118381507689359No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA31240.19071538415047712No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC27850.17001995674106235No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.15848179809687535No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT24340.14859194783042937No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC23570.14389121653094578No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23160.14138822973511683No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC22770.13900733985615762No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC22410.13680959535250295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA22350.13644330460189383No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC22220.13564967464224076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA22090.13485604468258772No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA21640.13210886405301936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA20320.12405046753961892No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT20180.123195789121531No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.12063175386726721No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC19160.1169688463611761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA18640.11379432652256381No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC18380.11220706660325767No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT16650.10164568329402829No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16620.10146253791872376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG255.176844E-9201.5740244
CGGGTAG100.0026462085201.57444
CGTTAAT301.5392288E-8167.9783544
TCTAGAC353.8649887E-8143.9814544
TAACGTA150.008897821134.3826844
GACTTAT451.1023076E-9134.3826844
CCCATAC3750.0120.94440544
CTATCAT15750.0117.10490444
ATAGACG451.7311322E-7111.98556544
CGCTTAG359.566065E-998.5102843
GACGAAC1000.091.94292443
GTGCGTC252.0503785E-591.94292443
CACGGGG209.736989E-486.1964943
TCGCCAG1200.083.98917444
CGGATAG753.8366124E-880.629644
CGACGGT150.001480261177.5742
TAATCGT651.5458281E-677.52846544
AATCGCA2350.077.19855544
AACGTAT305.0651965E-576.619143
CAGGCCG455.4722477E-876.619143
Produced by FastQC (version 0.11.5)