##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840981.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1638043
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.309831304794805	39.0	37.0	39.0	34.0	39.0
2	37.881932281386995	39.0	38.0	39.0	35.0	39.0
3	38.090681380159126	39.0	38.0	39.0	35.0	39.0
4	38.57149293394618	39.0	38.0	40.0	35.0	41.0
5	39.22172128570495	40.0	39.0	41.0	35.0	41.0
6	39.21341686390406	40.0	38.0	41.0	36.0	41.0
7	39.26029780658994	40.0	39.0	41.0	36.0	41.0
8	39.22881755851342	40.0	38.0	41.0	36.0	41.0
9	39.24640867181142	40.0	39.0	41.0	36.0	41.0
10	39.1988861098274	40.0	39.0	41.0	35.0	41.0
11	39.15134218088292	40.0	39.0	41.0	35.0	41.0
12	39.09351891250718	40.0	38.0	41.0	35.0	41.0
13	38.98424949772381	40.0	38.0	41.0	35.0	41.0
14	38.96016588087126	40.0	38.0	41.0	35.0	41.0
15	38.934485236346056	40.0	38.0	41.0	35.0	41.0
16	38.854318842667745	40.0	38.0	41.0	35.0	41.0
17	38.81087431770717	40.0	38.0	41.0	35.0	41.0
18	38.721288146892356	40.0	38.0	41.0	35.0	41.0
19	38.69376017601491	40.0	38.0	41.0	35.0	41.0
20	38.62995782162007	40.0	38.0	41.0	35.0	41.0
21	38.556968895199944	40.0	38.0	41.0	35.0	41.0
22	38.4820929609296	40.0	38.0	41.0	35.0	41.0
23	38.37878187568947	40.0	37.0	41.0	34.0	41.0
24	38.221220077861204	40.0	37.0	41.0	34.0	41.0
25	38.138739903188444	40.0	37.0	41.0	34.0	41.0
26	38.004634826084434	39.0	37.0	41.0	34.0	41.0
27	37.89795352331297	39.0	36.0	41.0	33.0	41.0
28	37.77458020797818	39.0	36.0	41.0	33.0	41.0
29	37.69891018333388	39.0	36.0	41.0	33.0	41.0
30	37.65731765899132	39.0	36.0	41.0	33.0	41.0
31	37.628743087856456	39.0	36.0	41.0	33.0	41.0
32	37.54189276301823	39.0	36.0	41.0	33.0	41.0
33	37.43322543566781	39.0	35.0	40.0	33.0	41.0
34	37.34533745248397	38.0	35.0	40.0	33.0	41.0
35	37.1733076533225	38.0	35.0	40.0	32.0	41.0
36	37.11759520639148	38.0	35.0	40.0	32.0	41.0
37	37.05574560398552	38.0	35.0	40.0	32.0	41.0
38	36.8495027926636	38.0	35.0	40.0	31.0	41.0
39	36.8175436455473	38.0	35.0	40.0	31.0	41.0
40	36.6050002971001	38.0	34.0	40.0	31.0	41.0
41	36.51747965716198	38.0	34.0	40.0	31.0	41.0
42	36.37059296902502	37.0	34.0	40.0	31.0	41.0
43	36.20755294646368	37.0	34.0	40.0	30.0	41.0
44	35.98498152017402	37.0	34.0	40.0	30.0	41.0
45	35.7411347917564	37.0	34.0	40.0	29.0	41.0
46	35.40572996001924	36.0	33.0	40.0	28.0	41.0
47	35.47082386239237	36.0	33.0	40.0	29.0	41.0
48	36.33578943838901	37.0	35.0	40.0	31.0	41.0
49	36.97366667021205	38.0	35.0	40.0	32.0	41.0
50	37.06646396326518	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	18.0
22	40.0
23	53.0
24	146.0
25	236.0
26	503.0
27	928.0
28	2067.0
29	4238.0
30	8925.0
31	17339.0
32	31997.0
33	55040.0
34	85668.0
35	99776.0
36	171904.0
37	284222.0
38	282927.0
39	261559.0
40	330451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.768159932309466	16.857188730698766	35.37465133699176
2	23.568428911817332	21.167331993116175	34.12895754262861	21.135281552437878
3	23.109649746679423	23.50976134326144	29.28238147594416	24.098207434114975
4	21.741431696237523	23.679964445377806	34.36338362301844	20.21522023536623
5	18.325587301432257	25.69670026977314	31.813389514194686	24.16432291459992
6	19.161768036614426	27.14373188005443	29.016027051792904	24.678473031538246
7	24.622186352861313	24.302902915247035	24.75429521691433	26.320615514977323
8	24.19954787511683	24.33238932067107	29.915453989913576	21.552608814298527
9	23.51427892918562	28.40108592997864	26.73299785170475	21.35163728913099
10	25.895657195812323	23.11325160572708	26.89477626655711	24.0963149319035
11	24.381411232794257	24.76937418614774	27.159482382330623	23.689732198727384
12	23.71097706226271	25.965008244594312	26.012870236007235	24.311144457135743
13	23.969944622943355	24.096803319570974	26.626407243277495	25.306844814208173
14	19.854057555265644	25.261424761132645	26.866449781843333	28.018067901758382
15	22.130554570301268	30.21098957719669	26.36548613192694	21.2929697205751
16	20.628029911302693	32.79584235578675	23.930751512628177	22.645376220282373
17	22.78108694338305	32.04305381482659	22.873392212536544	22.302467029253812
18	21.53514895518616	26.711569842794113	24.070003046318075	27.68327815570165
19	22.9868202483085	25.24842143948602	23.84497842852721	27.91977988367827
20	21.527334752506498	24.630672088583754	28.49003353391822	25.351959624991526
21	22.511680096310048	30.0424958319165	25.18371007354508	22.262113998228376
22	24.6284743440801	24.435622263884405	24.36950678339946	26.56639660863604
23	25.10208828461768	25.845597459895743	26.832689984328862	22.219624271157716
24	23.942839107398278	27.748294763934766	24.502104035119956	23.806762093546997
25	23.617010699352072	28.009066186475472	24.44243137298225	23.931491741190204
26	25.19778427283178	31.108653856839624	24.56568956852344	19.127872301805155
27	25.06113062426618	28.147885116714093	23.646793098644803	23.14419116037492
28	23.964182928541344	26.73407071659213	24.14756773831251	25.154178616554017
29	22.71256038036788	28.764562695738555	27.08440233862549	21.438474585268075
30	23.71850658824399	28.125577371251786	28.279691694900784	19.87622434560344
31	24.38262647328503	30.404699101838702	23.186276650117758	22.026397774758507
32	22.080120952918456	28.318762200141872	25.37299425784304	24.228122589096632
33	22.407226925621064	25.256860373636435	30.344946357768826	21.99096634297367
34	24.333396381557343	28.691210814872026	25.759993800257973	21.21539900331266
35	21.411161144935033	25.61389208308467	26.030718091969018	26.94422868001127
36	21.008921438082556	27.592676431424767	26.642343541944076	24.7560585885486
37	22.64320964329406	32.95808259670026	23.086060450140092	21.31264730986559
38	25.794399577326594	27.074819662939518	23.61963975717844	23.51114100255545
39	22.33601139021662	29.788264008949454	25.412115664937794	22.463608935896133
40	20.427031874401106	28.298886796666743	25.631815800671102	25.64226552826105
41	22.234410365844003	30.399571418045753	25.03658164575746	22.329436570352787
42	21.29226851045009	31.966002581537495	23.39297902344561	23.348749884566807
43	21.660510645469355	27.330135947418682	23.67619796640638	27.333155440705582
44	22.317277904108053	27.20014363263236	23.055970231993882	27.426608231265703
45	22.05103804212604	26.368953523946114	28.875604074444073	22.70440435948377
46	20.052590343417634	30.486258923377484	26.735414081703336	22.72573665150155
47	22.339047080694407	31.1315966513672	25.53939579709164	20.989960470846754
48	24.269096186869955	27.946667433076826	24.01222903209582	23.7720073479574
49	20.37318697993668	29.052638296514534	22.807092183494827	27.767082540053963
50	19.45797524122739	31.671062021035652	27.621234215506814	21.249728522230154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28275.0
1	27667.0
2	28513.0
3	29346.5
4	26901.5
5	18202.0
6	8620.5
7	6368.0
8	7118.0
9	7257.5
10	6673.5
11	5600.5
12	4616.0
13	4106.0
14	3967.5
15	4250.0
16	4958.5
17	5986.0
18	6562.0
19	6541.0
20	6882.5
21	8864.0
22	11463.0
23	13338.0
24	14920.0
25	17290.0
26	19697.0
27	21304.5
28	23075.0
29	26603.0
30	31871.0
31	38949.5
32	47536.5
33	50792.5
34	54169.0
35	63270.5
36	67496.0
37	66804.0
38	67344.0
39	68540.0
40	71339.5
41	80186.0
42	88295.0
43	89340.0
44	90833.0
45	92516.5
46	96526.0
47	100695.5
48	112110.5
49	127767.5
50	144909.5
51	156308.5
52	142695.5
53	126229.5
54	122633.0
55	120111.5
56	112699.5
57	106403.5
58	101709.5
59	94266.0
60	85766.5
61	75829.5
62	64162.0
63	52984.5
64	43258.5
65	37061.0
66	29014.5
67	20959.0
68	16564.0
69	13343.0
70	10289.5
71	7431.5
72	5545.0
73	3936.0
74	2458.0
75	1406.5
76	788.5
77	394.5
78	246.5
79	214.0
80	184.5
81	148.0
82	114.0
83	74.5
84	38.5
85	26.0
86	14.5
87	8.5
88	8.5
89	9.5
90	10.0
91	6.5
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8692.0
25	9657.0
26	11662.0
27	12480.0
28	13084.0
29	12854.0
30	12210.0
31	12340.0
32	12130.0
33	10290.0
34	11222.0
35	9422.0
36	9398.0
37	8714.0
38	8938.0
39	10797.0
40	11922.0
41	12004.0
42	16147.0
43	26069.0
44	67670.0
45	140769.0
46	184744.0
47	169766.0
48	270948.0
49	241804.0
50	322310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.495012417961103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.53915134953891	8.563975472290043
2	11.47647968013569	2.8679751223554746
3	4.281642601690358	1.6049753243217693
4	2.279704566435546	1.1393974746757913
5	1.3727401387661466	0.8576202540258325
6	1.032640673569561	0.774171482372603
7	0.7154580083009914	0.6257759688775425
8	0.5551146593957851	0.5548931650034066
9	0.4563918334943186	0.5132359464270794
>10	5.15898685702452	15.313152382662848
>50	2.4237635248585323	21.73035777187197
>100	1.6407014629799415	32.7418100390594
>500	0.040965017321522225	3.5093799387677023
>1k	0.02415885636910285	5.5157992444227855
>5k	0.0010503850595262108	1.1340944706567828
>10k+	0.0010503850595262108	2.55338594220897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23587	1.4399499891028502	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	15323	0.9354455285972346	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8665	0.5289848923379912	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8617	0.5260545663331183	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	4874	0.29755018641146785	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3753	0.2291148645059989	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3470	0.2118381507689359	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3124	0.19071538415047712	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2785	0.17001995674106235	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2596	0.15848179809687535	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2434	0.14859194783042937	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	2357	0.14389121653094578	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2316	0.14138822973511683	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2277	0.13900733985615762	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2241	0.13680959535250295	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2235	0.13644330460189383	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2222	0.13564967464224076	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2209	0.13485604468258772	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2164	0.13210886405301936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2032	0.12405046753961892	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	2018	0.123195789121531	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.12063175386726721	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	1916	0.1169688463611761	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1864	0.11379432652256381	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	1838	0.11220706660325767	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	1665	0.10164568329402829	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1662	0.10146253791872376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08687195635279416	0.0	0.0	0.0	0.0
2	0.15799341042939655	0.0	0.0	0.0	0.0
3	0.1934015163216106	0.0	0.0	0.0	0.0
4	0.22301001866251374	0.0	0.0	0.0	0.0
5	0.2500544857491531	0.0	0.0	0.0	0.0
6	0.2923610674445054	0.0	0.0	0.0	0.0
7	0.32044335832453724	0.0	0.0	0.0	0.0
8	0.35609565805049076	0.0	0.0	0.0	0.0
9	0.38466633659800137	0.0	0.0	0.0	0.0
10	0.4126875790195984	0.0	0.0	0.0	0.0
11	0.44241817827737123	0.0	0.0	0.0	0.0
12	0.4723929713688835	0.0	0.0	0.0	0.0
13	0.5015741345007426	0.0	0.0	0.0	0.0
14	0.531121588383211	0.0	0.0	0.0	0.0
15	0.5560293594246305	0.0	0.0	0.0	6.104845843485184E-5
16	0.5831959234281395	0.0	0.0	0.0	6.104845843485184E-5
17	0.6157958002323505	0.0	0.0	0.0	6.104845843485184E-5
18	0.6473578532431689	0.0	0.0	0.0	6.104845843485184E-5
19	0.6733034480779808	0.0	0.0	0.0	6.104845843485184E-5
20	0.7024235627514052	0.0	0.0	0.0	6.104845843485184E-5
21	0.726781897666911	0.0	0.0	0.0	6.104845843485184E-5
22	0.7504686995396336	0.0	0.0	0.0	6.104845843485184E-5
23	0.7778184089184472	0.0	0.0	0.0	6.104845843485184E-5
24	0.7997958539549939	0.0	0.0	0.0	6.104845843485184E-5
25	0.8259245941651104	0.0	0.0	0.0	6.104845843485184E-5
26	0.8546173696294908	0.0	0.0	0.0	6.104845843485184E-5
27	0.8836764358444803	0.0	0.0	0.0	6.104845843485184E-5
28	0.9138343743112971	0.0	0.0	0.0	6.104845843485184E-5
29	0.9425881982341123	0.0	0.0	0.0	6.104845843485184E-5
30	0.9705483921972744	0.0	0.0	0.0	6.104845843485184E-5
31	0.9970434231580001	0.0	0.0	0.0	6.104845843485184E-5
32	1.0261024893729895	0.0	0.0	0.0	6.104845843485184E-5
33	1.0521701811246713	0.0	0.0	0.0	6.104845843485184E-5
34	1.082816507258967	0.0	0.0	0.0	6.104845843485184E-5
35	1.1085789567184745	0.0	0.0	0.0	6.104845843485184E-5
36	1.1289691418357148	0.0	0.0	0.0	6.104845843485184E-5
37	1.14954247232826	0.0	0.0	0.0	6.104845843485184E-5
38	1.1627899878086227	0.0	0.0	0.0	6.104845843485184E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	25	5.176844E-9	201.57402	44
CGGGTAG	10	0.0026462085	201.574	44
CGTTAAT	30	1.5392288E-8	167.97835	44
TCTAGAC	35	3.8649887E-8	143.98145	44
TAACGTA	15	0.008897821	134.38268	44
GACTTAT	45	1.1023076E-9	134.38268	44
CCCATAC	375	0.0	120.944405	44
CTATCAT	1575	0.0	117.104904	44
ATAGACG	45	1.7311322E-7	111.985565	44
CGCTTAG	35	9.566065E-9	98.51028	43
GACGAAC	100	0.0	91.942924	43
GTGCGTC	25	2.0503785E-5	91.942924	43
CACGGGG	20	9.736989E-4	86.19649	43
TCGCCAG	120	0.0	83.989174	44
CGGATAG	75	3.8366124E-8	80.6296	44
CGACGGT	15	0.0014802611	77.57	42
TAATCGT	65	1.5458281E-6	77.528465	44
AATCGCA	235	0.0	77.198555	44
AACGTAT	30	5.0651965E-5	76.6191	43
CAGGCCG	45	5.4722477E-8	76.6191	43
>>END_MODULE