##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840980.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2961092
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.03289225731588	39.0	37.0	39.0	33.0	39.0
2	37.75246699528417	39.0	37.0	39.0	35.0	39.0
3	37.914815885490896	39.0	38.0	39.0	35.0	39.0
4	38.30474635708718	39.0	38.0	40.0	35.0	41.0
5	39.09542695735222	40.0	38.0	41.0	35.0	41.0
6	39.006514488573806	40.0	38.0	41.0	35.0	41.0
7	39.07359514665536	40.0	38.0	41.0	35.0	41.0
8	38.99864644529789	40.0	38.0	41.0	35.0	41.0
9	39.04886508085531	40.0	38.0	41.0	35.0	41.0
10	39.02596508315175	40.0	38.0	41.0	35.0	41.0
11	38.996750523117825	40.0	38.0	41.0	35.0	41.0
12	38.95798171755555	40.0	38.0	41.0	35.0	41.0
13	38.81067221146793	40.0	38.0	41.0	35.0	41.0
14	38.797966763612884	40.0	38.0	41.0	35.0	41.0
15	38.71895773586231	40.0	38.0	41.0	35.0	41.0
16	38.66356938588872	40.0	38.0	41.0	35.0	41.0
17	38.65569560148756	40.0	38.0	41.0	35.0	41.0
18	38.6194505270353	40.0	38.0	41.0	35.0	41.0
19	38.62820574301643	40.0	38.0	41.0	35.0	41.0
20	38.5893352857662	40.0	38.0	41.0	35.0	41.0
21	38.51148157504056	40.0	38.0	41.0	35.0	41.0
22	38.379995285523044	40.0	37.0	41.0	34.0	41.0
23	38.30873002257275	40.0	37.0	41.0	34.0	41.0
24	38.195296194782195	40.0	37.0	41.0	34.0	41.0
25	38.09981863924476	40.0	37.0	41.0	34.0	41.0
26	37.94303795093662	39.0	37.0	41.0	33.0	41.0
27	37.86848483290048	39.0	36.0	41.0	33.0	41.0
28	37.74376735412527	39.0	36.0	41.0	33.0	41.0
29	37.657333579794276	39.0	36.0	41.0	33.0	41.0
30	37.61603820435342	39.0	36.0	41.0	33.0	41.0
31	37.46536384803321	39.0	35.0	40.0	33.0	41.0
32	37.33130255983162	38.0	35.0	40.0	33.0	41.0
33	37.245733143766685	38.0	35.0	40.0	32.0	41.0
34	37.16512230829794	38.0	35.0	40.0	32.0	41.0
35	37.088748396044714	38.0	35.0	40.0	32.0	41.0
36	37.059093904382166	38.0	35.0	40.0	32.0	41.0
37	36.96551282323492	38.0	35.0	40.0	32.0	41.0
38	36.865568729137124	38.0	35.0	40.0	32.0	41.0
39	36.67474589494101	38.0	35.0	40.0	31.0	41.0
40	36.55494762167011	38.0	34.0	40.0	31.0	41.0
41	36.42188831745518	37.0	34.0	40.0	31.0	41.0
42	36.28732120559922	37.0	34.0	40.0	31.0	41.0
43	36.1562174209325	37.0	34.0	40.0	30.0	41.0
44	35.93043540136896	37.0	34.0	40.0	30.0	41.0
45	35.66938192880191	36.0	34.0	40.0	29.0	41.0
46	35.312175568911776	36.0	33.0	40.0	28.0	41.0
47	35.16959029114732	36.0	33.0	39.0	28.0	41.0
48	36.0060838296452	37.0	34.0	40.0	31.0	41.0
49	36.83238899194925	38.0	35.0	40.0	32.0	41.0
50	36.60395952789249	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	26.0
22	77.0
23	128.0
24	311.0
25	587.0
26	915.0
27	1743.0
28	3854.0
29	8031.0
30	16723.0
31	33759.0
32	62169.0
33	106800.0
34	166198.0
35	195618.0
36	329177.0
37	507805.0
38	509085.0
39	524288.0
40	493786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.785988412383	17.017809645900904	34.1962019417161
2	23.213496912625477	22.305217129356333	33.61692240565305	20.86436355236514
3	22.402782487001417	20.532155029293246	30.526609777744156	26.53845270596118
4	21.167494964695457	22.022179655343365	35.66717954052086	21.143145839440315
5	19.63974776872856	23.874638140253662	33.5881154655107	22.897498625507076
6	19.59790509717361	28.530656933320547	30.78455515735411	21.08688281215173
7	26.547131936461277	26.011316095548537	25.062173009146626	22.379378958843564
8	24.40342954558656	25.612578062417512	27.88960964400971	22.094382747986216
9	23.188911388096013	25.481815492392673	28.896603009970644	22.43267010954067
10	22.77744156547652	23.434867947365365	29.332725899769407	24.454964587388705
11	24.45023660190227	24.17287271047303	29.00777821155168	22.369112476073017
12	23.92293113486511	25.237682584668086	28.83193092278119	22.00745535768561
13	23.97341926559526	24.060279113246057	28.579186327206312	23.387115293952366
14	20.208456880096936	26.000272872305217	28.879852432818705	24.911417814779142
15	21.312441491179605	28.80325906793845	27.479862158960277	22.40443728192167
16	21.559782674769984	31.925316741256264	24.4483454077077	22.066555176266053
17	21.577411306369406	31.49655600028638	24.698861095838968	22.227171597505244
18	21.95176644292038	26.76522715268556	25.254804646393964	26.02820175800009
19	21.488761578498742	26.335351957993876	26.38979133373769	25.78609512976969
20	22.313592417932306	25.67508878481317	27.7928547981623	24.218463999092226
21	24.190467570747547	26.626595863958297	26.519304364741114	22.66363220055304
22	24.32916640212462	27.446529861280904	25.034379208751368	23.189924527843107
23	24.650838271826743	26.716056103626634	26.189628691036955	22.443476933509665
24	25.230995862337274	27.70835894325472	25.959409569172454	21.101235625235553
25	24.395973017753406	27.513301824192016	26.342971982375506	21.74775317567907
26	25.90591518607089	28.24311275244657	25.704466158371137	20.146505903111407
27	25.615298242005025	28.562764106388265	25.269699229430774	20.552238422175936
28	25.14775379038598	27.403453604654132	24.88423681190009	22.5645557930598
29	24.018719450077064	29.225565968376095	25.039262340535828	21.71645224101101
30	25.80692882323317	28.740977001846566	25.497453975714844	19.954640199205418
31	24.568921340977855	28.994717417588863	25.32219737677643	21.114163864656852
32	23.118327435269983	28.610496199925727	26.289093660914975	21.982082703889315
33	22.507528276112247	28.010042938130674	28.15064692831339	21.331781857443694
34	23.541833087174656	28.62284275919934	26.512439154746648	21.322884998879353
35	21.95284651670988	27.63271927772569	27.881940213730545	22.53249399183388
36	22.87860688226302	29.26506402430926	26.070198049451783	21.78613104397594
37	22.249332096488537	31.84909517286419	24.9530509041682	20.94852182647907
38	24.542213132254613	29.26202666030565	24.413336337145523	21.782423870294213
39	22.531463536970538	30.66990087698567	24.399457079416212	22.39917850662759
40	21.673688750785413	30.21619213496729	25.346029798710205	22.76408931553709
41	21.541213845727963	30.815765042192144	25.25110965061777	22.391911461462122
42	22.4406399929474	30.461109249164213	25.13347119340156	21.964779564486822
43	23.235784674883476	29.62341382915873	25.180675777729807	21.960125718227992
44	22.07475518129701	28.3326602048943	25.43862495694389	24.153959656864796
45	22.323614893470946	28.535262309606086	27.413086547294846	21.728036249628122
46	23.033403259787708	29.80834061765303	25.14498923970695	22.01326688285231
47	21.94956035783276	30.10012308570269	26.891161101229176	21.059155455235373
48	22.5255023008385	28.437833823493225	25.956285014988733	23.08037886067954
49	20.60838468655124	28.64259206258801	25.566438433664256	25.182584817196492
50	20.239647313670332	29.398481217900528	28.067426680600093	22.294444787829043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37721.0
1	33236.0
2	37495.5
3	44822.0
4	39776.0
5	26598.5
6	13730.0
7	10647.0
8	10451.0
9	9733.0
10	9156.0
11	8614.5
12	8278.0
13	8051.5
14	8273.5
15	8880.5
16	9465.5
17	10430.0
18	11477.5
19	12714.5
20	14285.5
21	16051.5
22	18013.5
23	21742.0
24	25986.0
25	29717.0
26	34453.5
27	42666.5
28	51246.0
29	58310.0
30	65974.0
31	79006.0
32	98060.5
33	108223.5
34	115051.0
35	122923.5
36	125126.0
37	130144.5
38	138614.0
39	149699.0
40	159363.0
41	167474.5
42	175801.5
43	180430.5
44	183632.5
45	187416.5
46	202593.0
47	215074.0
48	221509.0
49	229245.0
50	241188.0
51	249879.0
52	246779.5
53	233317.0
54	217300.5
55	206883.0
56	198361.5
57	193486.5
58	185654.5
59	162725.5
60	130958.0
61	108248.0
62	92036.0
63	73977.0
64	55725.5
65	44416.5
66	34049.5
67	24497.0
68	18416.5
69	14003.0
70	10807.0
71	8028.0
72	5763.0
73	4170.0
74	2977.5
75	2028.5
76	1465.0
77	951.0
78	506.5
79	334.5
80	237.5
81	162.0
82	94.5
83	64.0
84	54.0
85	41.0
86	29.5
87	28.5
88	26.0
89	21.5
90	12.5
91	2.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	13376.0
25	15957.0
26	15084.0
27	18949.0
28	19650.0
29	18716.0
30	20029.0
31	20044.0
32	18513.0
33	16662.0
34	18293.0
35	17566.0
36	16571.0
37	15134.0
38	17074.0
39	19358.0
40	25001.0
41	23461.0
42	28372.0
43	45573.0
44	110677.0
45	235661.0
46	367940.0
47	258574.0
48	533154.0
49	356014.0
50	695689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.625410406750962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91703230487514	11.862347866262448
2	10.612590380824603	3.3165216035824376
3	3.5442378216876853	1.6614051162899737
4	1.6582065914744866	1.0364063412387392
5	0.9167447551991106	0.7162256519111272
6	0.6089901831944924	0.5709432927657837
7	0.41221028435484725	0.4508668406849602
8	0.3259313712220063	0.4074249151823158
9	0.2740633198919876	0.38541166656740833
>10	2.812131961715579	10.333941744027825
>50	1.397362890579609	16.006732001426936
>100	1.441846122062273	37.10149564365684
>500	0.045450548448629585	5.030365529868077
>1k	0.03223443152385077	9.989007565753475
>5k	9.670329457155231E-4	1.130904220781672
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	9000	0.3039419241279906	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8043	0.27162276619571424	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC	5410	0.18270286772582547	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	4920	0.16615491852330153	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	4794	0.16189973158550966	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4741	0.16010985136564485	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4254	0.14366321613783023	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	3941	0.13309279144315678	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3887	0.13126913989838884	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	3704	0.12508898744111968	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3652	0.12333287854615797	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3603	0.12167808362590557	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3546	0.1197531181064283	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	3501	0.11823340848578835	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	3265	0.11026337580865439	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	3235	0.10925023606156106	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	3125	0.10553539032221897	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3085	0.10418453732609456	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3050	0.10300254095448572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06852201822841034	0.0	0.0	0.0	0.0
2	0.1254267006901508	0.0	0.0	0.0	0.0
3	0.15284901651147617	0.0	0.0	0.0	0.0
4	0.17763716899035897	0.0	0.0	0.0	0.0
5	0.19648156828629437	0.0	0.0	0.0	0.0
6	0.23531859192487095	0.0	0.0	0.0	0.0
7	0.2618290819738124	0.0	0.0	0.0	0.0
8	0.29256098763564253	0.0	0.0	0.0	0.0
9	0.3193078769589057	0.0	0.0	0.0	0.0
10	0.34186712199418323	0.0	0.0	0.0	0.0
11	0.3650004795528136	0.0	0.0	0.0	0.0
12	0.3903289732301462	0.0	0.0	0.0	0.0
13	0.41342855946387347	0.0	0.0	0.0	0.0
14	0.44014167746223354	0.0	0.0	0.0	0.0
15	0.46688856678549673	0.0	0.0	0.0	0.0
16	0.496438476075718	0.0	0.0	0.0	0.0
17	0.5251778735682647	0.0	0.0	0.0	0.0
18	0.5549304108079046	0.0	0.0	0.0	0.0
19	0.5828592965027767	0.0	0.0	0.0	0.0
20	0.6101127556995865	0.0	0.0	0.0	0.0
21	0.636657017073431	0.0	0.0	0.0	0.0
22	0.6610736849783796	0.0	0.0	0.0	0.0
23	0.6865034926304214	0.0	0.0	0.0	0.0
24	0.7123723275062038	0.0	0.0	0.0	0.0
25	0.7390516741796608	0.0	0.0	0.0	0.0
26	0.7684327268453665	0.0	0.0	0.0	0.0
27	0.7938963058223115	0.0	0.0	0.0	0.0
28	0.8231085018635017	0.0	0.0	0.0	0.0
29	0.8572850826654491	0.0	0.0	0.0	0.0
30	0.8890976707241788	0.0	0.0	0.0	0.0
31	0.9197282624112996	0.0	0.0	0.0	0.0
32	0.9518785637190604	0.0	0.0	0.0	0.0
33	0.9822727561318595	0.0	0.0	0.0	0.0
34	1.0105055837508594	0.0	0.0	0.0	0.0
35	1.0375564149982506	0.0	0.0	0.0	0.0
36	1.0596766328097877	0.0	0.0	0.0	0.0
37	1.0815266800221	0.0	0.0	0.0	0.0
38	1.0967237762284996	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACGG	45	3.5342964E-9	113.68813	44
CGTTTGA	310	0.0	100.34268	43
TGCGAAT	30	4.8402217E-7	94.26131	43
GGACGCA	25	2.2198452E-5	90.49085	43
CTATCAT	1905	0.0	90.4134	44
CCGATTG	60	2.6077032E-8	85.266106	44
CCCGACT	60	2.6077032E-8	85.266106	44
CGATTGC	20	0.001037502	84.835175	43
ACGAATA	20	0.001037502	84.835175	43
CGTTATA	30	6.586379E-8	74.60985	42
GCGTTAA	35	0.0018747366	73.085236	44
CAGTCGA	55	2.9776857E-9	71.98136	43
CCCGAGC	1345	0.0	71.63621	44
AATCGCA	280	0.0	70.040016	44
AACGCAC	170	0.0	65.20349	44
CACGGTA	80	1.9138861E-7	63.949577	44
CGTCGAT	40	0.0031832757	63.949577	44
TCGCAAA	775	0.0	63.48955	43
CCCTATC	1825	0.0	62.549625	42
CTAATCG	230	0.0	61.63422	42
>>END_MODULE