##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840979.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2806270
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.33916551151528	39.0	37.0	39.0	34.0	39.0
2	37.8772897832354	39.0	38.0	39.0	35.0	39.0
3	38.00316327366932	39.0	38.0	39.0	35.0	39.0
4	38.359782558342566	39.0	38.0	39.0	35.0	41.0
5	39.24277100920439	40.0	39.0	41.0	36.0	41.0
6	39.16399277332545	40.0	38.0	41.0	35.0	41.0
7	39.21870383106401	40.0	38.0	41.0	36.0	41.0
8	39.16551329700991	40.0	38.0	41.0	36.0	41.0
9	39.19204068033368	40.0	38.0	41.0	36.0	41.0
10	39.15047839302704	40.0	39.0	41.0	35.0	41.0
11	39.12381844940081	40.0	38.0	41.0	35.0	41.0
12	39.054697516632395	40.0	38.0	41.0	35.0	41.0
13	38.92060742551501	40.0	38.0	41.0	35.0	41.0
14	38.901551525690685	40.0	38.0	41.0	35.0	41.0
15	38.84421349335595	40.0	38.0	41.0	35.0	41.0
16	38.72568035149861	40.0	38.0	41.0	35.0	41.0
17	38.698222195298385	40.0	38.0	41.0	35.0	41.0
18	38.71164214419853	40.0	38.0	41.0	35.0	41.0
19	38.67097250086414	40.0	38.0	41.0	35.0	41.0
20	38.583569293047354	40.0	38.0	41.0	35.0	41.0
21	38.473981833537046	40.0	38.0	41.0	35.0	41.0
22	38.39787440267686	40.0	37.0	41.0	34.0	41.0
23	38.323739697178105	40.0	37.0	41.0	34.0	41.0
24	38.165032587741024	40.0	37.0	41.0	34.0	41.0
25	38.04209928141431	39.0	37.0	41.0	34.0	41.0
26	37.876360007864584	39.0	36.0	41.0	33.0	41.0
27	37.706841385946	39.0	36.0	41.0	33.0	41.0
28	37.624267030151984	39.0	36.0	41.0	33.0	41.0
29	37.50149071312811	39.0	35.0	41.0	33.0	41.0
30	37.44664684677796	39.0	35.0	40.0	33.0	41.0
31	37.33154049826705	38.0	35.0	40.0	33.0	41.0
32	37.243949846424826	38.0	35.0	40.0	33.0	41.0
33	37.11328930975374	38.0	35.0	40.0	32.0	41.0
34	36.99487859546407	38.0	35.0	40.0	32.0	41.0
35	36.88245808111281	38.0	35.0	40.0	32.0	41.0
36	36.83353837800101	38.0	35.0	40.0	32.0	41.0
37	36.762854833486145	38.0	35.0	40.0	32.0	41.0
38	36.609743918209844	38.0	35.0	40.0	31.0	41.0
39	36.49461445630157	37.0	34.0	40.0	31.0	41.0
40	36.31618695335408	37.0	34.0	40.0	31.0	41.0
41	36.19177774847048	37.0	34.0	40.0	31.0	41.0
42	36.016184985641075	36.0	34.0	40.0	31.0	41.0
43	35.86273213356981	36.0	34.0	40.0	30.0	41.0
44	35.59609709030956	36.0	34.0	39.0	30.0	41.0
45	35.40052890591859	36.0	33.0	39.0	29.0	41.0
46	35.00115622034106	36.0	33.0	39.0	28.0	41.0
47	34.83766509536554	35.0	33.0	39.0	28.0	41.0
48	35.66756122996266	36.0	34.0	39.0	30.0	41.0
49	36.499327779476054	37.0	35.0	40.0	32.0	41.0
50	36.14704446940797	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	11.0
21	21.0
22	65.0
23	126.0
24	267.0
25	520.0
26	834.0
27	1741.0
28	3890.0
29	8122.0
30	16442.0
31	31816.0
32	57915.0
33	99508.0
34	159149.0
35	197029.0
36	331841.0
37	497243.0
38	482601.0
39	437955.0
40	479170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.46452052012101	17.54342953457793	32.99204994530106
2	24.039347603758728	22.146942382593267	33.889468939196874	19.92424107445114
3	23.38385116186254	21.981348907981058	28.820854728874984	25.81394520128142
4	21.189051659320025	24.2567179922103	32.93977414860295	21.614456199866726
5	19.911412658083506	24.8999205350875	30.76770945062307	24.420957356205925
6	19.177484703895207	28.688971481717722	29.93553720775264	22.19800660663443
7	25.93481739105646	25.03978590798462	23.98379343398889	25.041603266970036
8	24.032897761085	24.817925573804374	27.853128886386557	23.296047778724073
9	23.959383808400474	26.107537763650683	28.75888635092133	21.174192077027513
10	25.13439547869592	23.72793779643441	26.077711695595934	25.059955029273734
11	24.614274463968187	24.39351879897515	27.63497453915696	23.3572321978997
12	24.535344068817327	24.723957423911454	27.349649178446832	23.39104932882438
13	22.838928542157383	25.179116763533088	26.37144679592484	25.61050789838469
14	20.682507385248034	23.76065025817188	26.52367733682076	29.033165019759327
15	21.35888563823153	29.891492978223766	25.80428825451578	22.945333129028924
16	21.463437231627747	31.429370659273697	25.764128184387104	21.34306392471145
17	21.474412654520055	31.961500497101134	22.55424460226564	24.009842246113166
18	22.94462043923072	26.210200729081663	24.811083751741634	26.034095079945978
19	23.35598499075285	25.300915450045792	24.86952431519419	26.47357524400717
20	22.666600148952167	25.22772220777046	26.05365841490662	26.052019228370753
21	22.78102249605348	29.010786560095784	26.211305398268873	21.996885545581858
22	25.396415883004842	27.486556888681417	24.073200369173314	23.043826859140427
23	24.747832532151218	26.90888617274888	25.18663564090412	23.156645654195785
24	24.868063301107878	27.262736657556115	25.764627067245844	22.10457297409016
25	22.923485018841927	27.955518443030925	25.767785246434645	23.353211291692507
26	26.39994296015205	29.43216543284386	25.151079976060547	19.016811630943543
27	24.32200920882541	29.820384004609128	23.571792158167433	22.285814628398025
28	24.836596052578688	27.677847738144397	23.444437541796546	24.04111866748037
29	23.16239061070033	30.115759246420463	25.566218512854704	21.155631630024498
30	24.060265924058076	29.613051875570495	26.272983640610896	20.053698559760527
31	24.290173047721147	30.211427154795427	25.02485651648308	20.473543281000346
32	22.774177071381377	28.73422718578113	25.475284454968232	23.016311287869257
33	22.191466519525044	27.703091681805226	29.160567917079522	20.944873881590205
34	22.841479516865935	29.595152608377457	26.402042294294453	21.161325580462155
35	21.57638195540486	27.055726408581265	25.692705591893105	25.675186044120768
36	22.198502889500833	28.236667571655744	26.81759298762751	22.74723655121592
37	21.629045257030256	33.2440540254407	23.613882098964865	21.513018618564182
38	24.10964699646796	29.75639024900434	22.971750359475468	23.162212395052233
39	22.350787549982062	30.707863475250946	24.95137762409534	21.989971350671656
40	20.85250174970513	29.92093364345371	25.74661720355494	23.479947403286218
41	21.842491684947987	31.98078370571471	24.170625832309366	22.00609877702794
42	22.163184098175524	30.846914829683307	24.368091656890478	22.62180941525069
43	22.42042365248093	28.348761051928683	24.634788001489724	24.59602729410066
44	21.24380071902069	29.298300185717824	23.990873390813846	25.46702570444764
45	22.435951375701904	27.97775246647819	28.26957459377822	21.316721564041686
46	21.297224688485443	29.793458959679146	26.296430594250374	22.612885757585037
47	22.73920369602993	30.88350774852161	25.69872914630042	20.67855940914804
48	23.182360307908333	29.590929552477473	23.46915977567853	23.75755036393566
49	21.32170723238055	29.586804494133034	22.900015729663476	26.19147254382294
50	19.881570853896957	29.118668834450435	26.664029132241545	24.33573117941106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46150.0
1	40067.0
2	37071.0
3	38712.0
4	33909.5
5	22088.0
6	10432.5
7	7627.0
8	8475.5
9	9150.0
10	9062.0
11	8382.5
12	7649.0
13	7176.0
14	7511.5
15	8207.5
16	8774.0
17	9621.5
18	10942.0
19	12325.0
20	13113.0
21	14526.5
22	17009.0
23	21211.0
24	25733.0
25	29408.0
26	32948.5
27	35902.5
28	39960.0
29	48429.5
30	57492.0
31	66164.5
32	79907.0
33	89058.5
34	94292.5
35	103143.5
36	112669.5
37	123176.5
38	130334.5
39	133303.5
40	137693.5
41	147273.5
42	159261.0
43	169550.5
44	176506.0
45	177571.5
46	184675.0
47	189801.5
48	197562.5
49	219154.5
50	244978.0
51	258883.0
52	252232.5
53	237114.5
54	224026.0
55	212128.0
56	195541.5
57	182909.0
58	174810.5
59	156167.5
60	132902.0
61	113115.5
62	94888.5
63	79591.5
64	63674.0
65	50870.0
66	37244.0
67	24771.5
68	17823.0
69	13540.5
70	10502.5
71	8000.5
72	6105.5
73	4410.0
74	2963.0
75	1934.5
76	1339.5
77	937.0
78	519.5
79	345.0
80	213.0
81	167.0
82	181.0
83	170.5
84	141.0
85	85.0
86	52.5
87	29.0
88	7.5
89	4.5
90	3.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	13562.0
25	15702.0
26	20737.0
27	19799.0
28	23339.0
29	19204.0
30	19578.0
31	19623.0
32	19623.0
33	18438.0
34	19566.0
35	18799.0
36	18543.0
37	16468.0
38	15132.0
39	17734.0
40	23367.0
41	22735.0
42	26285.0
43	39774.0
44	92748.0
45	215082.0
46	343260.0
47	238801.0
48	497542.0
49	318263.0
50	692566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.992323483341854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00676923916632	10.4951897863479
2	10.376518676981268	2.903832119185207
3	3.6140618693853668	1.5170736829575366
4	1.6264853035752382	0.9103323403410484
5	0.9353153076056263	0.6543617171469657
6	0.6216874126779239	0.5219310830226811
7	0.4557159353775981	0.4463567349021945
8	0.3513849507352293	0.3933353518292375
9	0.26959574121011526	0.3395043738668924
>10	3.249836720198999	11.15096156460097
>50	1.8667327022551397	19.116899324240286
>100	1.5394764448413691	35.039083558460646
>500	0.049909049234403584	4.840598862116771
>1k	0.034165926321538025	8.224237458666522
>5k	0.0013398402479034518	1.250175581410755
>10k+	0.001004880185927589	2.1961264609043916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	21129	0.75292113731038	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	13838	0.4931100713758833	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11890	0.4236940850310198	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8216	0.2927729691013338	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC	6761	0.24092478628214678	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6662	0.23739697178104743	No Hit
AGAGCTCGTATGCCGTCTTCTGCTTG	5035	0.1794196566973242	Illumina Single End Adapter 1 (100% over 26bp)
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4502	0.160426473575244	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4479	0.15960688030731185	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	3824	0.13626628941620014	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC	3184	0.11346021587373989	No Hit
AGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3106	0.11068072566075254	Illumina Single End Adapter 1 (100% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3024	0.10775869748812482	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2960	0.10547809013387878	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2827	0.10073870297583624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.039732456249755015	0.0	0.0	0.0	0.0
2	0.07971435392888068	0.0	0.0	0.0	0.0
3	0.10572753156324943	0.0	0.0	0.0	0.0
4	0.14118384902379316	0.0	0.0	0.0	0.0
5	0.1617093152120074	0.0	0.0	0.0	0.0
6	0.20261770962879552	0.0	0.0	0.0	0.0
7	0.23108966706696077	0.0	0.0	0.0	0.0
8	0.2607731971620692	0.0	0.0	0.0	0.0
9	0.2902785548076272	0.0	0.0	0.0	0.0
10	0.31561467713370417	0.0	0.0	0.0	0.0
11	0.3456545521279136	0.0	0.0	0.0	0.0
12	0.37573006161203304	0.0	0.0	0.0	0.0
13	0.41453602112412563	0.0	0.0	0.0	0.0
14	0.4395157985511016	0.0	0.0	0.0	0.0
15	0.4678808525195366	0.0	0.0	0.0	0.0
16	0.49788509302383593	0.0	0.0	0.0	0.0
17	0.5317022239485153	0.0	0.0	0.0	0.0
18	0.5656262583429249	0.0	0.0	0.0	0.0
19	0.5950959814985728	0.0	0.0	0.0	0.0
20	0.6231403250578169	0.0	0.0	0.0	0.0
21	0.6511133996372409	0.0	0.0	0.0	0.0
22	0.6753448527761049	0.0	0.0	0.0	0.0
23	0.701856913269215	0.0	0.0	0.0	0.0
24	0.728333339272415	0.0	0.0	0.0	0.0
25	0.7573398140592317	0.0	0.0	0.0	0.0
26	0.7891257790590357	0.0	0.0	0.0	0.0
27	0.8180966193559422	0.0	0.0	0.0	0.0
28	0.8488848186382636	0.0	0.0	0.0	0.0
29	0.8788534246526528	0.0	0.0	0.0	0.0
30	0.908786396177132	0.0	0.0	0.0	0.0
31	0.9390400781108019	0.0	0.0	0.0	0.0
32	0.9658728490131028	0.0	0.0	0.0	0.0
33	0.9938815580824368	0.0	0.0	0.0	0.0
34	1.0216051912324902	0.0	0.0	0.0	0.0
35	1.049542631322004	0.0	0.0	0.0	0.0
36	1.0722061669048237	0.0	0.0	0.0	0.0
37	1.0921971157443866	0.0	0.0	0.0	0.0
38	1.105524414970762	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCGA	30	3.3105607E-10	159.76413	44
ACGCGTG	25	3.990841E-6	127.8113	44
CACGGTA	20	2.6281676E-4	119.82309	44
CGTGCGC	95	0.0	109.312294	44
AAACGGT	20	0.0011478814	82.70254	43
TCGTGCG	220	0.0	80.1964	43
CTATCAT	2390	0.0	78.54513	44
CGAACGG	25	2.0452699E-6	73.983345	42
CCCGAGC	1520	0.0	72.524506	44
CGCGGTA	45	7.3854746E-5	71.00628	44
AACGACA	505	0.0	66.59875	43
CCTATCA	2000	0.0	65.33501	43
CTACCGC	50	1.2442413E-4	63.90565	44
ATGCGTC	50	1.2442413E-4	63.90565	44
CGGATAC	35	1.3343683E-4	63.011456	43
CCCTATC	2220	0.0	61.31953	42
TAACGGT	40	7.220369E-4	59.911545	44
AACGGTA	40	0.004121808	59.911545	44
GCCCGAG	1295	0.0	59.179684	43
ACCTTAG	3005	0.0	58.96509	42
>>END_MODULE