FastQCFastQC Report
Tue 24 May 2016
ERR840978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281962
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC1052872.4588494713404745No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT758461.771290824159579No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA708011.6534710023115573No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC447941.0461092368404952No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT381930.8919509327733409No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA275830.644167323297124No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA216420.5054225142586506No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG216170.5048386697499885No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA153590.3586907123416789No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC141400.33022245409931245No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG127580.2979475296604687No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA109640.2560508477188728No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC102980.2404972300081131No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG98610.2302916279966987No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA97680.2281197264244755No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG87530.2044156393727922No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT82590.19287887188162808No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT77310.1805480758586835No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT76160.1778623911188376No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC71060.1659519631421297No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT70740.16520464217104214No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG65080.15198640249493106No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG64060.14960431689958947No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA63240.14768930691117763No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT60440.14115024841416154No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC59890.1398657904951048No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA58160.13582558649516274No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG50580.11812342099252632No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG50350.11758628404455714No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA47530.11100051798684808No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44870.1047884124146828No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG44470.10385426120082335No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT44130.10306023266904285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT108300.0240.2547544
ACTTCTA45500.0166.6073244
GTACGTC502.4583642E-8155.3408244
GGTACGT259.188079E-8124.5713643
GAACACA29200.0113.8456744
TACCGAC900.0110.7300943
CCCATAC21700.0106.662644
AATCGCA5800.0101.7750344
CCTTAAC142200.099.1227143
CCTATCA109250.095.5522243
CGCTTAT207.0718373E-493.4285343
AAAGTCG855.8203295E-791.3769644
TCGTGCG9500.085.2330443
CCGCTTA150.001065035984.2804242
TACGCGA302.829438E-884.2804242
GTACGGT150.001065035984.2804242
GTACGTA951.4042598E-981.7583344
CAATGAA24150.080.2105443
TAATTCC9300.075.1649244
CCCTATC145100.074.9578742
Produced by FastQC (version 0.11.5)