##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840978.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4281962
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.212302911609214	39.0	37.0	39.0	34.0	39.0
2	37.778617605667684	39.0	38.0	39.0	35.0	39.0
3	38.08084284727422	39.0	38.0	39.0	35.0	40.0
4	38.71133046019558	39.0	38.0	41.0	35.0	41.0
5	39.14828459477221	40.0	38.0	41.0	35.0	41.0
6	39.1939891105993	40.0	38.0	41.0	35.0	41.0
7	39.194808594751656	40.0	38.0	41.0	35.0	41.0
8	39.13269010794584	40.0	38.0	41.0	35.0	41.0
9	39.131366882751415	40.0	38.0	41.0	35.0	41.0
10	39.097647994073746	40.0	38.0	41.0	35.0	41.0
11	38.96038404824704	40.0	38.0	41.0	35.0	41.0
12	38.923283532175205	40.0	38.0	41.0	35.0	41.0
13	38.80856719419743	40.0	38.0	41.0	35.0	41.0
14	38.740628711791466	40.0	38.0	41.0	35.0	41.0
15	38.7197095630461	40.0	38.0	41.0	35.0	41.0
16	38.72666758836253	40.0	38.0	41.0	35.0	41.0
17	38.72602979662127	40.0	38.0	41.0	35.0	41.0
18	38.65189228676014	40.0	38.0	41.0	35.0	41.0
19	38.64509260007445	40.0	38.0	41.0	35.0	41.0
20	38.488909289713455	40.0	37.0	41.0	35.0	41.0
21	38.411630462857914	40.0	37.0	41.0	35.0	41.0
22	38.382789945356826	40.0	37.0	41.0	34.0	41.0
23	38.23644838510944	40.0	37.0	41.0	34.0	41.0
24	38.02776671068076	39.0	36.0	41.0	34.0	41.0
25	37.92630139634126	39.0	36.0	41.0	34.0	41.0
26	37.81919515625898	39.0	36.0	41.0	33.0	41.0
27	37.72837906416907	39.0	36.0	41.0	33.0	41.0
28	37.49885334799068	39.0	35.0	41.0	33.0	41.0
29	37.49766930452659	39.0	35.0	41.0	33.0	41.0
30	37.41452337591652	38.0	35.0	40.0	33.0	41.0
31	37.312941651426364	38.0	35.0	40.0	33.0	41.0
32	37.26161799712357	38.0	35.0	40.0	33.0	41.0
33	37.05925332141549	38.0	35.0	40.0	32.0	41.0
34	36.954970723759544	38.0	35.0	40.0	32.0	41.0
35	36.75405172595432	38.0	35.0	40.0	32.0	41.0
36	36.71927063159852	38.0	35.0	40.0	32.0	41.0
37	36.64557303526151	37.0	35.0	40.0	32.0	41.0
38	36.350697254736595	37.0	34.0	40.0	31.0	41.0
39	36.3635176949958	37.0	34.0	40.0	31.0	41.0
40	36.05011104704087	36.0	34.0	40.0	31.0	41.0
41	35.98776804501273	36.0	34.0	40.0	31.0	41.0
42	35.88798365066725	36.0	34.0	40.0	30.0	41.0
43	35.75643296592536	36.0	34.0	40.0	30.0	41.0
44	35.33685229865337	36.0	33.0	39.0	29.0	41.0
45	35.13381905631183	36.0	33.0	39.0	29.0	41.0
46	34.7884986133052	35.0	32.0	39.0	28.0	40.0
47	35.19336629814471	36.0	34.0	39.0	29.0	40.0
48	36.048310268171065	36.0	34.0	39.0	31.0	41.0
49	36.72791524598861	38.0	35.0	39.0	32.0	41.0
50	36.6998120235636	37.0	35.0	39.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	33.0
22	102.0
23	192.0
24	380.0
25	754.0
26	1403.0
27	3082.0
28	6732.0
29	14118.0
30	28012.0
31	54854.0
32	102231.0
33	177275.0
34	275229.0
35	298670.0
36	476193.0
37	726373.0
38	698320.0
39	650942.0
40	767053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.65573304947592	15.154992034025524	36.18927491649856
2	26.08367379252782	20.12294831201211	32.55659438360266	21.236783511857414
3	25.130932969512575	26.22407671997089	22.21563386130003	26.429356449216506
4	21.254368908458318	26.48507856912322	26.135659307579097	26.124893214839368
5	14.03949871577562	30.2066202362375	26.719830769166098	29.034050278820782
6	21.14084618219405	28.096092398764867	23.603502319731	27.159559099310083
7	22.779160581060736	24.959866528474564	22.39779334800262	29.863179542462078
8	21.024824601432705	24.514930305313314	29.638562883089577	24.821682210164404
9	19.88768232880161	29.374548396272548	28.645910449462185	22.091858825463653
10	31.669360914459304	19.889503923668634	26.359692122442937	22.08144303942912
11	22.849945889290936	22.11381137898935	23.94526154132148	31.09098119039823
12	21.3634777702371	25.593594711956808	25.141605647130916	27.901321870675172
13	24.962925873699955	24.722335228570454	22.779651010448017	27.535087887281577
14	19.299213771630853	23.295909678787435	22.878344086192264	34.52653246338945
15	21.9885417012108	31.855443836260104	24.320860390634014	21.83515407189508
16	19.300638352231992	33.5741419470794	21.438770358074173	25.686449342614438
17	20.16290663018495	37.42858997814553	17.726430080416407	24.682073311253113
18	20.483110312515617	28.460738325094898	21.450096941542217	29.606054420847265
19	25.770406182960055	22.59489925412696	23.02710766699938	28.6075868959136
20	22.503422496509778	21.452735918721373	27.19809283688178	28.845748747887068
21	21.099323160738	34.62996635654403	21.084656986680407	23.186053496037566
22	26.78828069936165	25.613678963054788	19.52196212857564	28.076078209007925
23	29.18472886961631	22.864728832250265	26.768313217165403	21.182229080968025
24	22.617529067282707	26.533257417978017	24.159999551607417	26.689213963131852
25	22.269140168491752	23.946457399229367	23.04289141116182	30.741511021117063
26	21.735336264651487	33.71660459520393	25.83099289530634	18.71706624483824
27	23.271871247833726	29.482282381749464	20.020914537851407	27.224931832565403
28	24.544587131514014	24.446268681576235	20.789188777610423	30.219955409299327
29	24.437759921029205	30.13109907960665	25.106586901952966	20.324554097411184
30	26.012261421342963	29.962323175210447	25.372985193003057	18.652430210443537
31	25.785310400010093	29.507592170890685	22.273885872637468	22.433211556461753
32	20.768280889029345	30.052412603799826	23.301540482847514	25.87776602432331
33	20.658575191676025	23.35941832824315	31.263728595043027	24.718277885037804
34	24.35209785322362	29.63698250722754	23.877164778973075	22.13375486057577
35	19.839073707068042	23.583361530138355	23.53135546689571	33.046209295897896
36	19.996615216967584	23.55975986484796	28.362512755248282	28.08111216293618
37	19.083790866765966	35.11514612262647	21.510358817432014	24.29070419317555
38	25.286671840753012	23.31759695681099	23.779158709682623	27.616572492753384
39	20.824048120559784	29.943015793200146	28.134441690299262	21.098494395940808
40	18.18320245205356	23.76350467680112	30.157964511445396	27.895328359699928
41	19.677017852620363	29.809931542583573	23.653530396705715	26.85952020809035
42	19.677100158876836	31.721257481998155	22.654268786166938	25.947373572958064
43	19.216397087382976	22.393389230187033	24.32332496552514	34.066888716904856
44	18.87306339031875	24.907626824202147	22.11887123475103	34.10043855072807
45	18.733374640478395	22.78510551714783	34.54600267155302	23.935517170820756
46	18.516956417602582	29.5318874362441	29.383205483432302	22.56795066272101
47	20.72113655758734	28.779369109022333	28.67739237045312	21.822101962937207
48	18.273875465394855	29.58768914954565	22.742258222901004	29.396177162158494
49	18.323349005245245	28.814566255358145	22.750822446542802	30.111262292853812
50	16.023942837660197	29.133795100214595	34.42083748880984	20.42142457331537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45574.0
1	39200.5
2	31243.5
3	27320.0
4	21386.0
5	14084.5
6	8102.5
7	5840.5
8	5688.0
9	5290.5
10	4856.0
11	4413.5
12	3934.0
13	3368.5
14	3033.5
15	3117.5
16	3214.0
17	3172.5
18	3245.0
19	3533.0
20	3931.5
21	4896.0
22	6059.5
23	7904.0
24	10624.5
25	12956.5
26	15344.5
27	18573.5
28	22833.0
29	29301.5
30	40016.5
31	66247.5
32	112979.5
33	136619.0
34	139997.5
35	149116.5
36	140473.0
37	130599.0
38	134595.0
39	144127.0
40	163201.5
41	191394.5
42	221773.0
43	247183.5
44	268733.5
45	288456.5
46	307092.0
47	319362.5
48	424530.5
49	580453.0
50	588840.5
51	535130.0
52	502127.0
53	432414.0
54	358450.5
55	325208.5
56	304784.5
57	279436.5
58	245811.5
59	209493.5
60	187204.5
61	166792.5
62	145532.0
63	118240.0
64	89695.0
65	71555.5
66	56001.0
67	40476.5
68	29209.5
69	22125.0
70	17299.5
71	12577.5
72	9368.0
73	6916.5
74	4680.5
75	3200.5
76	2288.0
77	1490.0
78	1080.5
79	775.5
80	562.0
81	417.0
82	276.5
83	172.5
84	113.0
85	98.0
86	95.0
87	105.5
88	99.0
89	71.0
90	69.0
91	51.5
92	34.0
93	32.5
94	17.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18964.0
25	17354.0
26	18919.0
27	24055.0
28	20871.0
29	18497.0
30	41556.0
31	20824.0
32	24121.0
33	20440.0
34	22216.0
35	22071.0
36	20016.0
37	22275.0
38	22198.0
39	33478.0
40	32716.0
41	29980.0
42	43564.0
43	89235.0
44	238988.0
45	423436.0
46	447750.0
47	581933.0
48	658027.0
49	820005.0
50	548473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.110352325228549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41696610811582	7.321610416771858
2	11.954272293272908	2.4172380935341393
3	4.168016839410521	1.2642035623177772
4	1.8305449627105674	0.7402981808070477
5	1.08476650854081	0.5483685795977816
6	0.6703147738491374	0.40662711194524054
7	0.5119037049552827	0.36228687695814266
8	0.38919724617493573	0.3147937026269848
9	0.30690773180209463	0.2792650769870335
>10	3.030785193737298	7.204594909454923
>50	1.5810963932331306	11.90455437833699
>100	1.9174713438939754	33.047837507049174
>500	0.07722239541954982	5.365475813778957
>1k	0.05104531222648209	9.548022758221698
>5k	0.005235416638613548	3.749828606469262
>10k+	0.004253776018873508	15.524994425142994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	105287	2.4588494713404745	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	75846	1.771290824159579	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	70801	1.6534710023115573	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	44794	1.0461092368404952	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	38193	0.8919509327733409	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	27583	0.644167323297124	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	21642	0.5054225142586506	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	21617	0.5048386697499885	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	15359	0.3586907123416789	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	14140	0.33022245409931245	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	12758	0.2979475296604687	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	10964	0.2560508477188728	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	10298	0.2404972300081131	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	9861	0.2302916279966987	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9768	0.2281197264244755	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	8753	0.2044156393727922	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	8259	0.19287887188162808	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	7731	0.1805480758586835	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	7616	0.1778623911188376	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	7106	0.1659519631421297	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	7074	0.16520464217104214	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	6508	0.15198640249493106	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	6406	0.14960431689958947	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	6324	0.14768930691117763	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6044	0.14115024841416154	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	5989	0.1398657904951048	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	5816	0.13582558649516274	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	5058	0.11812342099252632	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	5035	0.11758628404455714	No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	4753	0.11100051798684808	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4487	0.1047884124146828	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	4447	0.10385426120082335	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	4413	0.10306023266904285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.029122164092068074	0.0	0.0	0.0	0.0
2	0.050093858843212526	0.0	0.0	0.0	0.0
3	0.06847328397589703	0.0	0.0	0.0	0.0
4	0.08528800582536697	0.0	0.0	0.0	0.0
5	0.10892203153601084	0.0	0.0	0.0	0.0
6	0.1389082855008989	0.0	0.0	0.0	0.0
7	0.16032370207862656	0.0	0.0	0.0	0.0
8	0.18629310582391903	0.0	0.0	0.0	0.0
9	0.2053731443669981	0.0	0.0	0.0	0.0
10	0.2217908519505778	0.0	0.0	0.0	0.0
11	0.2395397250139072	0.0	0.0	0.0	0.0
12	0.2658827892447434	0.0	0.0	0.0	0.0
13	0.2862939932675722	0.0	0.0	0.0	0.0
14	0.30460335705921726	0.0	0.0	0.0	0.0
15	0.32751341557912006	0.0	0.0	0.0	0.0
16	0.3489288321568477	0.0	0.0	0.0	0.0
17	0.3839127951158838	0.0	0.0	0.0	0.0
18	0.4310407238550926	0.0	0.0	0.0	0.0
19	0.4566364671148413	0.0	0.0	0.0	0.0
20	0.48309630024740996	0.0	0.0	0.0	0.0
21	0.5066369108366678	0.0	0.0	0.0	0.0
22	0.5258570720618259	0.0	0.0	0.0	0.0
23	0.5500982960614783	0.0	0.0	0.0	0.0
24	0.570836453009158	0.0	0.0	0.0	0.0
25	0.5906404587429781	0.0	0.0	0.0	0.0
26	0.6230321520835542	0.0	0.0	0.0	0.0
27	0.6438170165919268	0.0	0.0	0.0	0.0
28	0.6699966043603376	0.0	0.0	0.0	0.0
29	0.696876805539143	0.0	0.0	0.0	0.0
30	0.722822855504089	0.0	0.0	0.0	0.0
31	0.7445652250066676	0.0	0.0	0.0	0.0
32	0.7690166330294383	0.0	0.0	0.0	0.0
33	0.7936548712949811	0.0	0.0	0.0	0.0
34	0.8180128641963661	0.0	0.0	0.0	0.0
35	0.8384941295602343	0.0	0.0	0.0	0.0
36	0.8568034933518793	0.0	0.0	0.0	0.0
37	0.8727541253285293	0.0	0.0	0.0	0.0
38	0.8828663122185577	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	10830	0.0	240.25475	44
ACTTCTA	4550	0.0	166.60732	44
GTACGTC	50	2.4583642E-8	155.34082	44
GGTACGT	25	9.188079E-8	124.57136	43
GAACACA	2920	0.0	113.84567	44
TACCGAC	90	0.0	110.73009	43
CCCATAC	2170	0.0	106.6626	44
AATCGCA	580	0.0	101.77503	44
CCTTAAC	14220	0.0	99.12271	43
CCTATCA	10925	0.0	95.55222	43
CGCTTAT	20	7.0718373E-4	93.42853	43
AAAGTCG	85	5.8203295E-7	91.37696	44
TCGTGCG	950	0.0	85.23304	43
CCGCTTA	15	0.0010650359	84.28042	42
TACGCGA	30	2.829438E-8	84.28042	42
GTACGGT	15	0.0010650359	84.28042	42
GTACGTA	95	1.4042598E-9	81.75833	44
CAATGAA	2415	0.0	80.21054	43
TAATTCC	930	0.0	75.16492	44
CCCTATC	14510	0.0	74.95787	42
>>END_MODULE