FastQCFastQC Report
Tue 24 May 2016
ERR840977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498766
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG160640.6428773242472484No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT129130.5167750801795766No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC127910.5118926702220217No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA85670.3428492303801156No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC75970.30403006924217796No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA63330.25344510050160757No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG60600.24251970772773443No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA53160.21274501093739873No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT47440.1898537117921406No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA46820.18737248705961262No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG46000.1840908672520756No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA45440.18184976104205036No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC35330.14138979000034416No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG35160.14070945418658648No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC33350.13346587875775484No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.12870352806145113No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.1245414736714042No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC30960.12390115761139699No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA30310.12129987361761765No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG30290.12121983411011675No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA30030.12017932051260503No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.11577714760005538No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG27710.11089473764250035No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG25790.10321094492241371No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.10249058935490557No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT25340.10141005600364339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGT500.0205.7432144
AGCGTCA5150.0174.7818644
TATCGAC150.0042949594171.4526744
CTATCAT21950.0167.5471544
GTACGTA351.9913332E-6146.9594344
CGTGCGC1000.0141.4484644
CGTAGTT507.592098E-8128.5895144
CAAACGC704.3783075E-9110.21957444
GACGAAT704.3783075E-9110.21957444
AACGACA2400.0101.49567443
CCAGAAT8500.099.8459644
TCGTGCG3300.099.0675443
ACTTCTA14350.096.7781744
AATCGCA2700.095.2514844
AATAACG551.8836341E-593.5196444
TAATAGG355.74797E-791.5750443
CCTATCA21100.086.8877943
AGCGGAT758.505285E-785.7263344
CGACTCG1650.085.7263344
GACGATT302.9462142E-585.4700443
Produced by FastQC (version 0.11.5)