##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840977.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2498766
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.67027084568943	39.0	37.0	39.0	32.0	39.0
2	37.48026665962319	39.0	37.0	39.0	35.0	39.0
3	37.6556880476203	39.0	37.0	39.0	35.0	39.0
4	38.033116346228496	39.0	37.0	40.0	34.0	41.0
5	38.5418246446446	40.0	38.0	41.0	35.0	41.0
6	38.60778040040564	40.0	38.0	41.0	35.0	41.0
7	38.58323268365265	40.0	38.0	41.0	35.0	41.0
8	38.492981335587245	40.0	38.0	41.0	35.0	41.0
9	38.53182971114542	40.0	38.0	41.0	35.0	41.0
10	38.528063452119966	40.0	38.0	41.0	35.0	41.0
11	38.37069017266923	40.0	38.0	41.0	34.0	41.0
12	38.30071723402671	40.0	38.0	41.0	34.0	41.0
13	38.209681098590266	40.0	37.0	41.0	34.0	41.0
14	38.13591948986019	39.0	37.0	40.0	34.0	41.0
15	38.04851074490369	39.0	37.0	40.0	34.0	41.0
16	38.00948748302162	39.0	37.0	40.0	34.0	41.0
17	38.065123344883034	39.0	37.0	40.0	34.0	41.0
18	38.1351811254035	39.0	37.0	40.0	34.0	41.0
19	38.14370453255727	40.0	37.0	40.0	34.0	41.0
20	38.030743174831095	39.0	37.0	40.0	34.0	41.0
21	37.96334430674981	39.0	36.0	40.0	34.0	41.0
22	37.82798069126921	39.0	36.0	40.0	33.0	41.0
23	37.757239373354686	39.0	36.0	40.0	33.0	41.0
24	37.56758696092391	39.0	36.0	40.0	33.0	41.0
25	37.43301780753503	39.0	35.0	40.0	33.0	41.0
26	37.276935446585775	38.0	35.0	40.0	33.0	41.0
27	37.16923706998855	38.0	35.0	40.0	33.0	41.0
28	36.96954463188397	38.0	35.0	40.0	32.0	41.0
29	36.91624457069248	38.0	35.0	40.0	32.0	41.0
30	36.886839654799715	38.0	35.0	40.0	32.0	41.0
31	36.730340419143495	38.0	35.0	40.0	32.0	41.0
32	36.656493673956945	38.0	35.0	40.0	31.0	41.0
33	36.51259783907182	38.0	35.0	40.0	31.0	41.0
34	36.38340523531637	37.0	34.0	40.0	31.0	41.0
35	36.26257607394355	37.0	34.0	40.0	31.0	41.0
36	36.24798127099082	37.0	34.0	40.0	31.0	41.0
37	36.11754363098189	37.0	34.0	40.0	31.0	41.0
38	35.96736207979726	36.0	34.0	40.0	31.0	41.0
39	35.863831254597926	36.0	34.0	40.0	31.0	41.0
40	35.64279296005422	36.0	34.0	39.0	30.0	41.0
41	35.513068915442155	36.0	34.0	39.0	30.0	41.0
42	35.37968580108143	36.0	33.0	39.0	29.0	41.0
43	35.24944475440067	35.0	33.0	39.0	29.0	41.0
44	34.942119259481665	35.0	33.0	39.0	29.0	41.0
45	34.77446649366509	35.0	33.0	39.0	28.0	41.0
46	34.498074170687225	35.0	32.0	39.0	28.0	40.0
47	34.66004213425243	35.0	33.0	39.0	28.0	40.0
48	35.441802717327484	36.0	34.0	39.0	30.0	40.0
49	35.961428358528096	36.0	34.0	39.0	31.0	41.0
50	36.00872166445665	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	22.0
22	34.0
23	84.0
24	176.0
25	448.0
26	1033.0
27	2438.0
28	5344.0
29	11409.0
30	23037.0
31	43713.0
32	78321.0
33	130525.0
34	192142.0
35	225345.0
36	343818.0
37	446572.0
38	391744.0
39	376172.0
40	226384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.833411371853146	16.57125957372559	32.59532905442126
2	28.72409821487886	20.848290716297566	28.463489578455924	21.964121490367646
3	25.947407640411306	20.558267560868043	27.369069372642336	26.125255426078315
4	21.13006980245449	21.17517206493125	31.258028963096184	26.436729169518074
5	17.16963493180234	25.27919781204002	32.063026309786515	25.48814094637113
6	22.96945772433273	26.402472260307686	27.990336029864345	22.637733985495238
7	26.851293798618997	23.425963055364125	25.62116660783763	24.101576538179245
8	23.450575203920653	22.148892693433478	28.59519458804866	25.805337514597205
9	23.5962471075723	23.312266934959098	28.534324542594224	24.557161414874383
10	27.534430995139203	20.76544982603413	28.014988198174617	23.68513098065205
11	26.06978804738019	21.041746205927243	28.302810267147866	24.585655479544705
12	23.870142302240385	24.269019187871134	27.925504028788612	23.935334481099872
13	26.20985718550677	23.22490381252186	26.94982243235261	23.615416569618763
14	22.179507805052573	23.89995701878447	26.7008195245173	27.219715651645654
15	22.458125330663215	25.840034641098846	27.609548072928796	24.092291955309143
16	21.93630776151108	29.311107962890482	25.466610318853387	23.28597395674505
17	24.803362939947156	28.95497217426522	22.055286489411174	24.18637839637645
18	23.743599840881462	26.239831981065855	24.166288479993725	25.850279698058966
19	26.694616462685982	22.697443458090913	25.37756636675863	25.23037371246447
20	25.40874175493023	23.94053704908743	26.633226160432788	24.017495035549548
21	25.77912457589066	25.857323174719042	24.58813670427723	23.77541554511307
22	27.321245766910547	26.494637753194976	22.071214351403853	24.112902128490624
23	28.59359379789864	24.58209372146091	24.823612935344887	22.000699545295557
24	25.50586969728258	25.974180855670358	24.217593804301803	24.302355642745262
25	25.267591928195177	24.40222677694039	26.41353773491889	23.91664355994554
26	24.470023639073172	27.28857523067225	26.966559152539833	21.27484197771474
27	27.61925272323793	25.897191737852648	23.82448982416658	22.659065714742837
28	27.490971234295035	25.37686174707642	23.419166088267772	23.71300093036077
29	27.99813380456144	27.680839292497232	23.826196099156082	20.494830803785238
30	30.783850745772295	25.78044197608873	23.354354079715325	20.081353198423653
31	25.56968463028062	25.999826256519597	25.2089138839596	23.221575229240184
32	24.330761619130527	26.844736525691864	26.502294489104948	22.32220736607266
33	24.89351009756679	24.53589955345849	28.275459598155518	22.295130750819204
34	25.53037067228066	23.836370889886343	27.10429874842193	23.528959689411067
35	23.04630380038371	23.62325988662511	26.558969935447145	26.771466377544034
36	25.58986533054732	23.4673609819602	26.981281930663283	23.961491756829194
37	23.118497845421665	26.66829004948529	25.7068100175343	24.50640208755875
38	23.85066497715971	24.78685946922506	25.58272017718527	25.779755376429957
39	24.60504176156578	25.67164899112508	26.645839856600535	23.077469390708604
40	21.75847232311361	25.279526728757677	28.33827826315799	24.623722684970716
41	23.829036910231963	26.608230885764613	25.4871025442896	24.07562965971383
42	23.71970414940198	28.669664110023803	24.060314364218755	23.550317376355462
43	23.40021097704147	24.659000329913372	25.37903975200193	26.561748941043223
44	21.698439695051484	27.594489694311598	25.131234692070453	25.57583591856647
45	22.29003205617463	24.462168625655117	27.61756474838447	25.63023456978578
46	20.99754149449433	26.383688450795262	27.046388420785338	25.57238163392507
47	23.775894150402515	26.00202838084087	27.61552563373159	22.606551835025023
48	23.841372943811443	26.41589973437014	24.88897803217901	24.853749289639406
49	20.171579267937094	27.610266245925963	25.063532278025804	27.15462220811114
50	21.49028645997769	28.883048136221085	27.963524236492603	21.663141167308623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15001.0
1	14725.0
2	22809.5
3	30201.5
4	26959.5
5	17402.5
6	7764.5
7	5618.5
8	5396.0
9	4636.5
10	4204.5
11	3869.0
12	3411.5
13	3020.5
14	3003.5
15	3092.5
16	3037.5
17	2945.5
18	2786.0
19	3124.0
20	3820.5
21	4773.5
22	5962.0
23	7827.5
24	10094.0
25	11799.5
26	13722.5
27	15982.0
28	18775.5
29	22943.0
30	27847.5
31	36840.0
32	50256.5
33	57547.0
34	63694.5
35	73363.5
36	79285.5
37	82260.0
38	83971.5
39	93218.5
40	108505.0
41	117969.5
42	129626.0
43	148837.0
44	164543.0
45	172384.0
46	177337.0
47	186505.5
48	208025.0
49	237058.5
50	267489.0
51	281907.0
52	272242.5
53	250222.5
54	223930.5
55	214362.5
56	209904.0
57	196389.5
58	172594.0
59	145846.5
60	129478.5
61	113564.0
62	96658.0
63	78822.0
64	65370.0
65	58536.0
66	48358.5
67	35545.0
68	25822.0
69	19511.0
70	14597.5
71	10641.0
72	7885.5
73	5917.0
74	4177.0
75	3094.5
76	2523.5
77	1938.5
78	1710.5
79	1550.0
80	1361.5
81	989.0
82	500.5
83	264.0
84	172.0
85	104.5
86	69.5
87	53.5
88	34.0
89	26.0
90	38.0
91	48.5
92	42.5
93	42.0
94	25.5
95	8.5
96	8.0
97	8.0
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	13919.0
25	14784.0
26	13704.0
27	18595.0
28	15724.0
29	16480.0
30	34248.0
31	17629.0
32	21537.0
33	16030.0
34	14901.0
35	15990.0
36	14247.0
37	17066.0
38	15131.0
39	19673.0
40	20341.0
41	20911.0
42	28807.0
43	54138.0
44	129611.0
45	232540.0
46	277412.0
47	288687.0
48	395006.0
49	391567.0
50	380088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.787539035995565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.93458065973867	8.361441385286456
2	10.794343718858128	2.544774959079874
3	3.9742922797061335	1.4054137616247555
4	1.913174301541777	0.9020646704834899
5	1.1511839061685087	0.6784812615785576
6	0.7653738956429017	0.5413124803213619
7	0.5795768258320203	0.47822491211973395
8	0.4698091386870764	0.44303148493930933
9	0.34944013359620574	0.3707135293957902
>10	4.587740606419058	13.66423855859899
>50	2.636035893995214	22.49983224844314
>100	1.753992623591027	33.10319989046129
>500	0.05590414736545083	4.51007062034566
>1k	0.03144608289306609	7.035719320132554
>5k	0.0019411162279670425	1.550095095357603
>10k+	0.0011646697367802257	1.9113858218314395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	16064	0.6428773242472484	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	12913	0.5167750801795766	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12791	0.5118926702220217	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	8567	0.3428492303801156	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7597	0.30403006924217796	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6333	0.25344510050160757	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	6060	0.24251970772773443	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5316	0.21274501093739873	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4744	0.1898537117921406	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4682	0.18737248705961262	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	4600	0.1840908672520756	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4544	0.18184976104205036	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	3533	0.14138979000034416	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	3516	0.14070945418658648	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3335	0.13346587875775484	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3216	0.12870352806145113	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3112	0.1245414736714042	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	3096	0.12390115761139699	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3031	0.12129987361761765	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	3029	0.12121983411011675	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA	3003	0.12017932051260503	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2893	0.11577714760005538	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	2771	0.11089473764250035	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	2579	0.10321094492241371	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2561	0.10249058935490557	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.10141005600364339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.022731220130256295	0.0	0.0	0.0	0.0
2	0.04194070193047288	0.0	0.0	0.0	0.0
3	0.058508879983159685	0.0	0.0	0.0	0.0
4	0.07287597157957168	0.0	0.0	0.0	0.0
5	0.08712300391473231	0.0	0.0	0.0	0.0
6	0.11589720686130674	0.0	0.0	0.0	0.0
7	0.13374601703400799	0.0	0.0	0.0	0.0
8	0.1526753605579714	0.0	0.0	0.0	0.0
9	0.17120450654443034	0.0	0.0	0.0	0.0
10	0.18689225001460721	0.0	0.0	0.0	0.0
11	0.20334036880604267	0.0	0.0	0.0	0.0
12	0.22451081854003135	0.0	0.0	0.0	0.0
13	0.2406387793014632	0.0	0.0	0.0	0.0
14	0.256406562279141	0.0	0.0	0.0	0.0
15	0.2752558662956035	0.0	0.0	0.0	0.0
16	0.2947054666183228	0.0	0.0	0.0	0.0
17	0.31451524472479614	0.0	0.0	0.0	0.0
18	0.3430093093951174	0.0	0.0	0.0	0.0
19	0.3614984356278259	0.0	0.0	0.0	0.0
20	0.3831891421605705	0.0	0.0	0.0	0.0
21	0.4039994141108051	0.0	0.0	0.0	0.0
22	0.42232846132851176	0.0	0.0	0.0	0.0
23	0.444259286383759	0.0	0.0	0.0	0.0
24	0.4647894200577405	0.0	0.0	0.0	0.0
25	0.48648012659048506	0.0	0.0	0.0	0.0
26	0.5096115442582458	0.0	0.0	0.0	0.0
27	0.53038179645473	0.0	0.0	0.0	0.0
28	0.5539934511674963	0.0	0.0	0.0	0.0
29	0.5797261528290364	0.0	0.0	0.0	0.0
30	0.6035779260643054	0.0	0.0	0.0	0.0
31	0.6286703116658383	0.0	0.0	0.0	0.0
32	0.6553634874173893	0.0	0.0	0.0	0.0
33	0.6805359125264231	0.0	0.0	0.0	0.0
34	0.7050280018216992	0.0	0.0	0.0	0.0
35	0.7258782935256842	0.0	0.0	0.0	0.0
36	0.7432068468996297	0.0	0.0	0.0	0.0
37	0.7588945903698066	0.0	0.0	0.0	0.0
38	0.7692196868374229	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAGT	50	0.0	205.74321	44
AGCGTCA	515	0.0	174.78186	44
TATCGAC	15	0.0042949594	171.45267	44
CTATCAT	2195	0.0	167.54715	44
GTACGTA	35	1.9913332E-6	146.95943	44
CGTGCGC	100	0.0	141.44846	44
CGTAGTT	50	7.592098E-8	128.58951	44
CAAACGC	70	4.3783075E-9	110.219574	44
GACGAAT	70	4.3783075E-9	110.219574	44
AACGACA	240	0.0	101.495674	43
CCAGAAT	850	0.0	99.84596	44
TCGTGCG	330	0.0	99.06754	43
ACTTCTA	1435	0.0	96.77817	44
AATCGCA	270	0.0	95.25148	44
AATAACG	55	1.8836341E-5	93.51964	44
TAATAGG	35	5.74797E-7	91.57504	43
CCTATCA	2110	0.0	86.88779	43
AGCGGAT	75	8.505285E-7	85.72633	44
CGACTCG	165	0.0	85.72633	44
GACGATT	30	2.9462142E-5	85.47004	43
>>END_MODULE