FastQCFastQC Report
Tue 24 May 2016
ERR840976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623613
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC153232.4571328692634697No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA92271.4796035361674629No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT59670.9568434269330498No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT54870.879872613303443No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA47100.755276108740517No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT38700.620577184888705No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC29650.4754551300245505No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA26960.432319403219625No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT24200.3880611853826011No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC23110.3705823964542112No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA22140.3550278778665615No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA20170.323437773106077No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA17420.27933991113078144No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA15490.24839122981721035No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA15120.2424580629332615No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG15030.24101486017770635No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC14740.23636454018758427No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG14670.23524204915548586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA13550.2172821926419109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA13390.21471649885425736No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG12770.2047744354270998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA12000.19242703407401704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA11680.18729564649870994No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG11610.1861731554666115No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA11430.18328674995550126No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC10770.1727032630814303No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10720.17190148377278858No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC10690.17142041618760354No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG10590.16981685757032006No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC9910.15891265897279241No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT9880.15843159138760737No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC9360.1500930865777333No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC9300.14913095140736324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9120.14624454589625296No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAG9100.14592383417279625No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT8780.14079244659748916No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT8370.13421785626662688No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG8370.13421785626662688No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA7930.1271621983505796No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG7540.12090831974317405No Hit
ATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTT7170.11497515285922519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7050.11305088251848502No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC7000.11224910320984328No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA6990.11208874734811494No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC6790.10888163011354798No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.10631593632589444No Hit
AAAAAAAAAAAAAAAAAAAAAAAA6560.105193445293796No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG6490.10407095426169756No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC6330.101505260474044No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC6310.1011845487505873No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC6270.10054312530367392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATC100.003472777184.0296844
GCATTCT100.003472777184.0296844
CCATCCA206.48095E-7184.0296844
GTCAAGC100.003472777184.0296844
CGATCAC100.003472777184.0296844
CGTAGTG154.7481226E-5184.0296644
TGGTCGA154.7481226E-5184.0296644
AGAACCG600.0168.6938644
GGCCGTG302.6433554E-8153.3580644
TGAGTAC302.6433554E-8153.3580644
TATAACT251.9689141E-6147.2237244
CCTTGAA251.9689141E-6147.2237244
GTCGCGC409.113137E-10138.0222544
CCCGCCT201.4941453E-4138.0222544
GTCAGTA304.877209E-6122.6864544
GCTTATG452.068191E-9122.6864444
TTTAACG556.184564E-11117.1097944
GCCTTGA206.3659572E-6116.317843
GAGTCTT152.9549468E-4116.3177943
CGACGAC356.548362E-11116.3177943
Produced by FastQC (version 0.11.5)