##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840976.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623613
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.622142258099174	39.0	37.0	39.0	32.0	39.0
2	37.395949090221016	39.0	37.0	39.0	35.0	39.0
3	37.658990431565734	39.0	37.0	39.0	35.0	39.0
4	38.09901974461725	39.0	37.0	40.0	35.0	41.0
5	38.77522918861538	40.0	38.0	41.0	35.0	41.0
6	38.76117880801074	40.0	38.0	41.0	35.0	41.0
7	38.806142591639365	40.0	38.0	41.0	35.0	41.0
8	38.757710310721556	40.0	38.0	41.0	35.0	41.0
9	38.77055641880461	40.0	38.0	41.0	35.0	41.0
10	38.78213892269725	40.0	38.0	41.0	35.0	41.0
11	38.65122600074084	40.0	38.0	41.0	35.0	41.0
12	38.66971342803951	40.0	38.0	41.0	35.0	41.0
13	38.61052768303419	40.0	38.0	41.0	35.0	41.0
14	38.56386893794709	40.0	38.0	41.0	35.0	41.0
15	38.432426841646986	40.0	38.0	41.0	35.0	41.0
16	38.46591716336895	40.0	38.0	41.0	35.0	41.0
17	38.45606329566574	40.0	38.0	41.0	35.0	41.0
18	38.457721375276016	40.0	38.0	41.0	35.0	41.0
19	38.4514482539652	40.0	37.0	41.0	35.0	41.0
20	38.41132882091939	40.0	37.0	41.0	35.0	41.0
21	38.3279950866964	40.0	37.0	41.0	34.0	41.0
22	38.26806047981681	40.0	37.0	41.0	34.0	41.0
23	38.206549574816435	40.0	37.0	41.0	34.0	41.0
24	38.04884118836522	40.0	36.0	41.0	34.0	41.0
25	37.979052591160915	39.0	36.0	41.0	34.0	41.0
26	37.77560425855316	39.0	36.0	41.0	33.0	41.0
27	37.67858591210272	39.0	36.0	40.0	33.0	41.0
28	37.593163074935234	39.0	36.0	40.0	33.0	41.0
29	37.549905694083236	39.0	35.0	40.0	33.0	41.0
30	37.55020041337875	39.0	35.0	40.0	33.0	41.0
31	37.49612520714169	39.0	35.0	40.0	33.0	41.0
32	37.50548671245148	39.0	35.0	40.0	33.0	41.0
33	37.386699953521436	38.0	35.0	40.0	33.0	41.0
34	37.29648209241835	38.0	35.0	40.0	33.0	41.0
35	37.14022230259526	38.0	35.0	40.0	32.0	41.0
36	37.10138467193082	38.0	35.0	40.0	32.0	41.0
37	36.998857357165264	38.0	35.0	40.0	32.0	41.0
38	36.77747495385657	38.0	35.0	40.0	32.0	41.0
39	36.80616244909393	38.0	35.0	40.0	32.0	41.0
40	36.611939126025696	38.0	34.0	40.0	31.0	41.0
41	36.506311522245795	37.0	34.0	40.0	31.0	41.0
42	36.410177301485206	37.0	34.0	40.0	31.0	41.0
43	36.1170383907543	37.0	34.0	40.0	31.0	41.0
44	35.788546884302534	36.0	34.0	39.0	30.0	41.0
45	35.57290020393187	36.0	34.0	39.0	29.0	41.0
46	35.257699566930256	36.0	33.0	39.0	29.0	41.0
47	35.192508547763346	36.0	33.0	39.0	29.0	40.0
48	35.9431843350605	37.0	34.0	39.0	31.0	41.0
49	36.91204380245804	37.0	35.0	40.0	33.0	41.0
50	36.55616434220854	37.0	35.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	21.0
22	32.0
23	54.0
24	118.0
25	198.0
26	328.0
27	640.0
28	1267.0
29	2517.0
30	4649.0
31	8805.0
32	15470.0
33	26155.0
34	37793.0
35	40560.0
36	68330.0
37	102664.0
38	107852.0
39	108951.0
40	97205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.74680130144817	15.390153829378155	38.86304486917367
2	22.23478343139094	28.879769985551935	30.480121485600847	18.405325097456274
3	24.03205192964226	28.408323752070597	18.996236447925234	28.563387870361908
4	17.88064071788112	31.798727736593047	25.849845978194807	24.47078556733102
5	13.451130749358978	33.46642869856786	27.38813975975485	25.694300792318316
6	18.93786691425612	32.817949593738426	23.62073914430905	24.623444347696406
7	20.521701760547007	33.49417026264687	19.983387132724943	26.000740844081182
8	19.067113738809166	30.15091090147255	28.221509173157074	22.560466186561218
9	16.73024776584196	37.38440346817658	26.86682285327599	19.018525912705474
10	27.490767511260987	25.45521020248135	24.931167246353105	22.122855039904557
11	17.56618287303183	32.000776122370766	22.8890353472426	27.544005657354802
12	17.98070277559961	29.84126373247511	24.48281225696065	27.695221234964635
13	18.51436708343155	31.29873815972406	23.14929291082771	27.037601846016678
14	14.756267107965998	32.2882941824497	23.843313080387997	29.11212562919631
15	15.710063773526208	39.80834267406228	26.334601748199603	18.146991804211908
16	14.887598558721516	44.34994138993254	19.79176187795957	20.970698173386378
17	17.97492996457739	43.35332970929086	18.747845218108026	19.923895108023725
18	18.236470375056324	32.24788450529415	20.894368783203685	28.62127633644584
19	18.03698768306626	27.587622451744913	24.29150771391873	30.083882151270096
20	16.719022855520972	29.83998088558128	26.78151353483651	26.659482724061235
21	16.931334016449302	39.383559996343884	23.24999639199311	20.4351095952137
22	20.900301950087634	31.42574000221291	19.913632332873114	27.760325714826344
23	22.43089865028471	28.445205600268114	25.92761857113306	23.196277178314116
24	17.533951344824434	32.6503777182323	23.761371235044813	26.054299701898454
25	18.635030443438446	30.030749970153494	21.22135138536198	30.112868201046076
26	17.547531974874797	38.09573300248783	25.423354487525092	18.933380535112278
27	19.383777136764717	32.920272365141976	21.79640640228148	25.899544095811827
28	16.93722765358525	28.08390082470285	23.136342960360206	31.842528561351696
29	19.171935888594387	30.23672031440845	27.883143752129154	22.70820004486801
30	21.264570763913877	28.70923742500188	30.252964360727347	19.773227450356895
31	18.228637222624002	36.578988625961536	22.303365009364576	22.88900914204989
32	18.52876706750998	31.038607182605393	24.1915836477646	26.241042102120037
33	17.5456583552056	27.549998632983375	31.463938101487315	23.440404910323707
34	18.545560195364814	35.28978942066184	24.013849515456066	22.15080086851728
35	16.24369874416166	31.829107691988213	23.331562434564717	28.595631129285415
36	16.610314752008026	29.89612783087806	25.744028177699274	27.74952923941464
37	15.087441969691925	39.85650368817768	18.704993103835037	26.351061238295358
38	23.05214780706316	29.522276656095254	22.0413113725269	25.38426416431469
39	16.892393830813663	34.1195222231481	27.45656694957174	21.531516996466493
40	16.667856913309574	32.07845629771401	25.345469088104437	25.908217700871976
41	16.79010921466025	35.22880282058261	22.79466237907347	25.18642558568367
42	18.15005396448156	38.013162245939945	21.56380709423979	22.272976695338702
43	17.70743895555674	26.641870760994067	25.581211493876708	30.069478789572486
44	20.285510912782257	28.72538027116212	21.359604394035863	29.62950442201976
45	18.318659864981672	26.89151259689731	33.990161424635204	20.79966611348581
46	16.399083324727602	33.01788747217785	27.243404530203925	23.339624672890626
47	15.858111082699116	35.61695475550892	24.804462499242103	23.720471662549855
48	21.038298244026915	30.237426028580966	24.46102638170968	24.26324934568244
49	15.425003747002396	28.724052008393286	21.82769409472422	34.0232501498801
50	17.762482642033447	35.19289983698605	25.00981102457284	22.034806496407654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19070.0
1	16986.0
2	11045.0
3	6750.5
4	5582.0
5	3817.5
6	2191.0
7	1880.0
8	2483.0
9	2881.0
10	2778.0
11	2434.5
12	2207.5
13	1921.5
14	1608.5
15	1446.5
16	1363.5
17	1385.0
18	1500.0
19	1701.0
20	2045.5
21	2797.0
22	3449.5
23	4044.0
24	4875.5
25	5056.5
26	5392.5
27	6928.0
28	7775.0
29	7441.0
30	8569.0
31	12591.5
32	18867.0
33	24689.0
34	36057.5
35	49066.0
36	47345.5
37	39365.0
38	36900.0
39	38161.5
40	40855.5
41	42048.0
42	42645.0
43	42490.0
44	41117.0
45	43200.0
46	47273.0
47	48328.0
48	52386.5
49	60159.0
50	69516.5
51	73581.0
52	61006.0
53	43230.0
54	32468.0
55	27215.5
56	24617.0
57	21454.5
58	17552.0
59	14764.5
60	12524.0
61	10305.5
62	8768.0
63	7851.5
64	6685.0
65	5324.5
66	3793.0
67	2164.0
68	1141.5
69	751.0
70	509.5
71	413.5
72	366.0
73	323.5
74	258.5
75	199.0
76	174.0
77	130.0
78	94.5
79	71.0
80	46.5
81	32.0
82	21.5
83	19.5
84	17.0
85	14.5
86	11.5
87	7.0
88	3.5
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3775.0
25	4042.0
26	4264.0
27	4768.0
28	4999.0
29	4250.0
30	4322.0
31	3769.0
32	4208.0
33	3535.0
34	3820.0
35	3790.0
36	3464.0
37	3354.0
38	3224.0
39	3921.0
40	5329.0
41	4417.0
42	6455.0
43	11458.0
44	26888.0
45	50438.0
46	75784.0
47	58776.0
48	107059.0
49	81000.0
50	132504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.85797441682582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.90526788386711	8.859821716352931
2	11.367604509627856	2.9232873593077757
3	4.225281851740996	1.6298569786069244
4	2.1500548737902823	1.1058140224786845
5	1.3705976254614387	0.8811554601972699
6	0.9690212511224184	0.7475790273775562
7	0.7794572483288437	0.7015568950615205
8	0.607353087897835	0.6247464372936421
9	0.5025940337224384	0.5816107104887166
>10	5.482390501845755	17.300312854286233
>50	2.2074229272672854	20.231457650818697
>100	1.3593734410855034	27.92372833792753
>500	0.0374139479197845	3.4402746575199683
>1k	0.031178289933153743	7.275345446615128
>5k	0.0037413947919784497	3.3163195764039552
>10k+	0.0012471315973261498	2.4571328692634697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	15323	2.4571328692634697	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	9227	1.4796035361674629	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	5967	0.9568434269330498	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	5487	0.879872613303443	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	4710	0.755276108740517	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3870	0.620577184888705	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	2965	0.4754551300245505	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2696	0.432319403219625	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	2420	0.3880611853826011	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	2311	0.3705823964542112	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2214	0.3550278778665615	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2017	0.323437773106077	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	1742	0.27933991113078144	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	1549	0.24839122981721035	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1512	0.2424580629332615	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	1503	0.24101486017770635	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	1474	0.23636454018758427	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1467	0.23524204915548586	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	1355	0.2172821926419109	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	1339	0.21471649885425736	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	1277	0.2047744354270998	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1200	0.19242703407401704	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1168	0.18729564649870994	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1161	0.1861731554666115	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1143	0.18328674995550126	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1077	0.1727032630814303	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1072	0.17190148377278858	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1069	0.17142041618760354	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1059	0.16981685757032006	No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC	991	0.15891265897279241	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	988	0.15843159138760737	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	936	0.1500930865777333	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	930	0.14913095140736324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	912	0.14624454589625296	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAG	910	0.14592383417279625	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	878	0.14079244659748916	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	837	0.13421785626662688	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	837	0.13421785626662688	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	793	0.1271621983505796	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	754	0.12090831974317405	No Hit
ATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTT	717	0.11497515285922519	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	705	0.11305088251848502	No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC	700	0.11224910320984328	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	699	0.11208874734811494	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	679	0.10888163011354798	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.10631593632589444	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	656	0.105193445293796	No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG	649	0.10407095426169756	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	633	0.101505260474044	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	631	0.1011845487505873	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	627	0.10054312530367392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05436063712590982	0.0	0.0	0.0	0.0
2	0.12331365766909927	0.0	0.0	0.0	0.0
3	0.1746275334221705	0.0	0.0	0.0	0.0
4	0.22305500366413145	0.0	0.0	0.0	0.0
5	0.2604179194468364	0.0	0.0	0.0	0.0
6	0.32905022826656916	0.0	0.0	0.0	0.0
7	0.3922304377875381	0.0	0.0	0.0	0.0
8	0.4478739218072747	0.0	0.0	0.0	0.0
9	0.4918114279208419	0.0	0.0	0.0	0.0
10	0.5387956954072478	0.0	0.0	0.0	0.0
11	0.5857799628936536	0.0	0.0	0.0	0.0
12	0.6346885007207996	0.0	0.0	0.0	0.0
13	0.6720514165035045	0.0	0.0	0.0	0.0
14	0.7079711295306544	0.0	0.0	0.0	0.0
15	0.7475790273775563	0.0	0.0	0.0	0.0
16	0.7860644341923597	0.0	0.0	0.0	0.0
17	0.8335297692639505	0.0	0.0	0.0	0.0
18	0.8829193746762816	0.0	0.0	0.0	0.0
19	0.9223669166614551	0.0	0.0	0.0	0.0
20	0.9605316117528018	0.0	0.0	0.0	0.0
21	0.9940459868540265	0.0	0.0	0.0	0.0
22	1.0180993661132787	0.0	0.0	0.0	0.0
23	1.0463219977774678	0.0	0.0	0.0	0.0
24	1.074544629441657	0.0	0.0	0.0	0.0
25	1.1050122431700429	0.0	0.0	0.0	0.0
26	1.1402905327502795	0.0	0.0	0.0	0.0
27	1.1715599257873073	0.0	0.0	0.0	0.0
28	1.2050743008885318	0.0	0.0	0.0	0.0
29	1.2397111670218548	0.0	0.0	0.0	0.0
30	1.2680941545477724	0.0	0.0	0.0	0.0
31	1.3033724441280088	0.0	0.0	0.0	0.0
32	1.3322364992391114	0.0	0.0	0.0	0.0
33	1.3575727253921903	0.0	0.0	0.0	0.0
34	1.3865971363650214	0.0	0.0	0.0	0.0
35	1.414178344582297	0.0	0.0	0.0	0.0
36	1.4388731472884626	0.0	0.0	0.0	0.0
37	1.4595590534514193	0.0	0.0	0.0	0.0
38	1.4743117927304275	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATC	10	0.003472777	184.02968	44
GCATTCT	10	0.003472777	184.02968	44
CCATCCA	20	6.48095E-7	184.02968	44
GTCAAGC	10	0.003472777	184.02968	44
CGATCAC	10	0.003472777	184.02968	44
CGTAGTG	15	4.7481226E-5	184.02966	44
TGGTCGA	15	4.7481226E-5	184.02966	44
AGAACCG	60	0.0	168.69386	44
GGCCGTG	30	2.6433554E-8	153.35806	44
TGAGTAC	30	2.6433554E-8	153.35806	44
TATAACT	25	1.9689141E-6	147.22372	44
CCTTGAA	25	1.9689141E-6	147.22372	44
GTCGCGC	40	9.113137E-10	138.02225	44
CCCGCCT	20	1.4941453E-4	138.02225	44
GTCAGTA	30	4.877209E-6	122.68645	44
GCTTATG	45	2.068191E-9	122.68644	44
TTTAACG	55	6.184564E-11	117.10979	44
GCCTTGA	20	6.3659572E-6	116.3178	43
GAGTCTT	15	2.9549468E-4	116.31779	43
CGACGAC	35	6.548362E-11	116.31779	43
>>END_MODULE