FastQCFastQC Report
Tue 24 May 2016
ERR840975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639719
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC213843.34271766197346No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA134142.096858151782267No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT96461.507849540188739No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT85451.3357427245399933No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC53640.8384931508990665No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34780.5436762078350026No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGTGT31080.48583831338447037No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA28140.43988063509134473No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC25720.4020515257480237No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG24770.3872012555512655No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT23060.3604707691971006No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATG20110.31435677227032494No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA19870.31060512506272286No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGT19040.29763067846976565No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG18880.2951295803313642No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC18170.28403095734220807No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACAC17860.27918507969905537No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT14320.22384828338692458No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG14220.2222850970504237No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT14180.22165982251582333No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA13870.21681394487267067No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGAC13700.21415652810061916No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC13610.21274966039776838No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA13330.20837273865556594No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATG12470.19492933616165847No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG12180.19039609578580594No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC11790.18429966907345255No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA11700.18289280137060177No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG11330.17710901192554857No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT11020.17226313428239587No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG10760.1681988498074936No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAA10690.167104619371943No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT10660.16663566347099273No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAAC10190.15928868768943866No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT10050.15710022681833744No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC9660.15100380010598405No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG9500.14850270196758264No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT9310.14553264792823098No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG8870.13865462804762715No Hit
AAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGA8650.13521561810732524No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA8540.1334961131371743No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA8450.13208924543432352No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC8400.13130765226607308No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAG7920.12380435785086889No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTA7860.12286644604896838No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA7710.12052166654421706No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA7530.1177079311385155No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTT7400.11567578890106438No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA7260.11348732802996316No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAG6990.10926672492141079No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT6890.10770353858490994No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGG6760.1056713963474588No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG6710.10488980317920836No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT6630.10363925411000768No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGA6550.102388705040807No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA6450.10082551870430612No Hit
CCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC6440.10066920007065602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC100.00338585185.5970344
GGGTCCA100.00338585185.5970344
TTGAGGA351.8189894E-12185.5970344
TTAAGCA351.8189894E-12185.5970344
TGGGGTC100.00338585185.5970344
CGTACTG206.213322E-7185.5970344
TCGTAGG800.0185.5970344
CTACAGC100.00338585185.5970344
GTCCAGT100.00338585185.5970344
CAGTAGT100.00338585185.5970344
TCAAGGT100.00338585185.5970344
CTATACG154.5904773E-5185.5970244
ATGACCA154.5904773E-5185.5970244
AGCGACG600.0170.130644
CTGGCAA500.0167.0373244
TCGGGCG450.0164.9751344
GGCATAT302.5131158E-8154.6641844
TGTATCA251.8876799E-6148.4776244
TGTATAT251.8876799E-6148.4776244
TGAAAGC251.8876799E-6148.4776244
Produced by FastQC (version 0.11.5)