##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840975.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639719
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.17042013759166	35.0	33.0	39.0	23.0	39.0
2	35.33770921295131	35.0	35.0	39.0	31.0	39.0
3	35.8570341040363	35.0	35.0	39.0	33.0	39.0
4	36.047686562381294	35.0	35.0	39.0	33.0	39.0
5	36.825351443368106	35.0	35.0	40.0	33.0	41.0
6	36.757665474997616	35.0	35.0	40.0	33.0	41.0
7	36.85217572090246	36.0	35.0	40.0	34.0	41.0
8	36.73235592502333	35.0	35.0	40.0	33.0	41.0
9	36.83331118819357	36.0	35.0	40.0	34.0	41.0
10	36.741869477067276	36.0	35.0	40.0	34.0	41.0
11	36.64571475913643	36.0	35.0	40.0	33.0	41.0
12	36.610020962328775	35.0	35.0	40.0	33.0	41.0
13	36.49204259995404	35.0	35.0	40.0	33.0	41.0
14	36.391831413479984	35.0	35.0	40.0	33.0	41.0
15	36.334117010750035	35.0	35.0	40.0	33.0	41.0
16	36.3105957459447	35.0	35.0	39.0	33.0	41.0
17	36.23902525952801	35.0	35.0	39.0	33.0	41.0
18	35.94393944841407	35.0	35.0	39.0	33.0	41.0
19	35.866016172725836	35.0	35.0	39.0	33.0	40.0
20	35.692299275150496	35.0	35.0	38.0	33.0	40.0
21	35.53715459443912	35.0	35.0	38.0	33.0	40.0
22	35.28678060210812	35.0	34.0	37.0	32.0	39.0
23	35.22146755059644	35.0	34.0	37.0	32.0	39.0
24	34.97883758337645	35.0	34.0	37.0	32.0	39.0
25	34.94090322134704	35.0	34.0	36.0	32.0	39.0
26	34.7910010935732	35.0	34.0	36.0	32.0	38.0
27	34.598555957826214	35.0	34.0	36.0	31.0	38.0
28	34.461307413992024	35.0	34.0	36.0	31.0	37.0
29	34.423031803018574	35.0	34.0	35.0	31.0	37.0
30	34.39965612838736	35.0	34.0	35.0	31.0	37.0
31	34.32771481853393	35.0	34.0	35.0	31.0	37.0
32	34.2183190881789	35.0	34.0	35.0	31.0	37.0
33	34.14879304567162	35.0	34.0	35.0	31.0	36.0
34	34.115379574311355	35.0	34.0	35.0	31.0	36.0
35	33.907850603905445	35.0	34.0	35.0	31.0	36.0
36	33.975349160137384	35.0	34.0	35.0	31.0	36.0
37	33.85638417183439	35.0	33.0	35.0	31.0	36.0
38	33.73426129575323	35.0	33.0	35.0	30.0	36.0
39	33.690467080923575	35.0	33.0	35.0	30.0	36.0
40	33.58097422150601	35.0	33.0	35.0	30.0	36.0
41	33.47423966528592	35.0	33.0	35.0	30.0	36.0
42	33.496795788801144	35.0	33.0	35.0	30.0	35.0
43	33.25071780038211	35.0	32.0	35.0	29.0	36.0
44	32.981853217219154	35.0	32.0	35.0	28.0	35.0
45	32.933597349871725	34.0	32.0	35.0	28.0	35.0
46	32.774104221941734	34.0	32.0	35.0	27.0	35.0
47	32.94625065749804	34.0	32.0	35.0	29.0	35.0
48	33.47755840878184	35.0	33.0	35.0	30.0	35.0
49	34.11216736082409	35.0	34.0	35.0	32.0	35.0
50	33.66803312598253	35.0	33.0	35.0	31.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	5.0
21	14.0
22	32.0
23	108.0
24	260.0
25	528.0
26	1168.0
27	2361.0
28	4473.0
29	8278.0
30	14601.0
31	26050.0
32	46540.0
33	86526.0
34	151723.0
35	84802.0
36	87281.0
37	81118.0
38	22129.0
39	13429.0
40	8290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.895708897187674	21.213376498118706	35.89091460469363
2	25.14604068348759	22.86941610300773	34.80575064989472	17.17879256360996
3	24.570319155754323	28.441393799465075	21.161009755845924	25.827277288934674
4	20.048958996059206	36.28264910062074	23.386674461755867	20.281717441564187
5	14.693169969939927	35.482922970866895	22.24195310753628	27.5819539516569
6	17.585846285634787	29.456683325022393	25.89590116910706	27.06156922023576
7	20.463359693865588	29.297707274600253	19.13934086684935	31.099592164684807
8	17.621955890007957	26.585422662137592	33.89550724615027	21.897114201704184
9	19.883417563023762	35.83776626925259	27.405001258365004	16.87381490935864
10	32.12691822503318	20.725974998397735	25.877299251702702	21.269807524866387
11	18.663975901919436	24.54687136070681	23.99428499075375	32.79486774662
12	15.256229688347538	29.628633822037486	26.46724577509813	28.647890714516844
13	21.081443571318033	28.254905669520525	24.06322150819344	26.600429250968006
14	15.499461482307076	27.044061533266948	22.43109865425288	35.02537833017309
15	18.862500566655047	39.01650568452711	24.349909882307703	17.77108386651014
16	17.100945884052216	39.64943983217631	21.243858631680475	22.005755652090997
17	20.15713149054507	43.70997265987098	15.977327545375392	20.155568304208565
18	18.249262566845754	29.567356917646652	22.414216241818675	29.769164273688915
19	23.933164404996567	23.386205505854914	21.193680350278793	31.486949738869725
20	18.979895860526263	26.232298868722047	25.068975597098103	29.71882967365359
21	15.312660715095221	41.764587264095645	23.099829769007954	19.82292225180118
22	20.3070410602155	30.124789165242866	20.336897919242666	29.231271855298967
23	24.551560919716316	25.44429663649196	29.200320765836253	20.80382167795548
24	16.860684144132033	29.051349107967717	25.96858933375435	28.119377414145895
25	18.736627466751287	24.08988227457954	24.23503611565808	32.93845414301109
26	18.416594499263578	39.073433756645194	26.34452612881407	16.165445615277154
27	19.26555055101191	33.51430215223631	21.35294323321774	25.867204063534043
28	18.11820205372588	26.456482742397743	20.800815473513914	34.624499730362466
29	18.374558872515966	30.893648246995987	29.580041807540486	21.15175107294756
30	19.126766668715447	30.855441291119828	29.73858640623433	20.279205633930392
31	22.87447346800381	35.262483535686805	19.855635685502275	22.00740731080711
32	16.048228499822038	31.17171110794005	25.563204364249504	27.216856027988406
33	16.47162918612502	28.41901611308377	32.54443437479881	22.564920325992396
34	21.021964658120154	37.79517238747655	21.393102510033334	19.789760444369968
35	15.667666779026343	28.251727947188726	22.410582278987963	33.670022994796966
36	15.863475367607599	30.0086535365461	24.559776483846733	29.56809461199957
37	13.614799398930419	42.61050387642657	19.80932399910278	23.96537272554023
38	25.140348376235472	24.446243347453027	24.385318236124686	26.028090040186814
39	16.60711296642333	35.565344528973505	26.808900943863524	21.018641560739653
40	14.527927993072018	30.562829591293884	25.883859904654177	29.02538251097992
41	16.199160434794695	38.43408579133191	21.371730004726032	23.99502376914737
42	16.630128186000466	40.03886126318413	19.66811708930192	23.66289346151348
43	18.36270355466846	24.16470094319148	22.852024606232675	34.620570895907385
44	15.868836589795004	30.00633217728641	19.983114193902914	34.141717039015674
45	15.591345315622384	24.680948083772563	38.008227046559256	21.719479554045794
46	17.782901007186833	31.727887827035197	29.37520929393885	21.114001871839125
47	20.261203125200456	37.36769856440915	24.076616162264102	18.29448214812629
48	24.35128578954461	27.88154074219276	22.11968440268062	25.647489065582008
49	18.031893391954434	30.61256932111859	20.233818259443794	31.121719027483184
50	19.20464546022099	29.64865698361001	29.67272672568768	21.47397083048132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13411.0
1	11101.0
2	6808.0
3	4374.0
4	3415.5
5	2312.5
6	1322.0
7	946.5
8	993.5
9	1107.0
10	1164.0
11	1046.0
12	920.5
13	840.0
14	805.0
15	830.5
16	857.0
17	873.0
18	935.0
19	1231.0
20	1653.0
21	2181.0
22	2972.0
23	4032.0
24	5107.0
25	6132.5
26	6515.5
27	6733.0
28	7838.5
29	9116.5
30	10676.0
31	14394.5
32	21092.0
33	26251.5
34	33763.5
35	42667.5
36	40860.5
37	34584.0
38	32299.0
39	33519.5
40	37595.5
41	40807.0
42	43434.5
43	47972.5
44	50947.5
45	50800.5
46	50668.5
47	49319.5
48	53586.5
49	66279.0
50	81222.0
51	86299.0
52	68498.5
53	44114.5
54	31928.5
55	29384.0
56	28064.0
57	24937.5
58	20791.0
59	16844.0
60	14187.5
61	11573.5
62	9483.0
63	7690.0
64	6184.0
65	5897.5
66	5145.0
67	3583.5
68	2168.5
69	1444.5
70	1073.5
71	838.0
72	742.0
73	667.5
74	571.5
75	495.5
76	424.0
77	340.0
78	208.5
79	113.5
80	75.0
81	44.5
82	29.5
83	25.5
84	19.5
85	10.0
86	6.0
87	5.0
88	4.5
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	3153.0
25	3778.0
26	3582.0
27	4293.0
28	3494.0
29	3165.0
30	4806.0
31	3767.0
32	3900.0
33	3040.0
34	3475.0
35	2978.0
36	3345.0
37	3695.0
38	3782.0
39	4633.0
40	5297.0
41	5817.0
42	7585.0
43	12560.0
44	27637.0
45	56085.0
46	76117.0
47	63243.0
48	106321.0
49	87224.0
50	132947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.71561263617307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.23017599103366	7.876426993726933
2	11.7950018012729	2.763713442933538
3	4.3737574552683895	1.5372374433149556
4	2.445728314675704	1.1461282219224378
5	1.4903865398214737	0.8730395689357358
6	1.0180527572818125	0.7156267048500983
7	0.8325883624427929	0.68279979178358
8	0.640452586494456	0.6002635532163341
9	0.5603960131826491	0.5908844351973288
>10	5.882823862195952	16.166629410725648
>50	2.145516164756428	17.78640309260785
>100	1.4837151587121566	27.98087910473192
>500	0.054705325096401455	4.318145935950003
>1k	0.0400282866559035	7.840161070720113
>5k	0.004002828665590351	3.682085415627799
>10k+	0.0026685524437269005	5.439575813755727
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	21384	3.34271766197346	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	13414	2.096858151782267	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	9646	1.507849540188739	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	8545	1.3357427245399933	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5364	0.8384931508990665	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3478	0.5436762078350026	No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGTGT	3108	0.48583831338447037	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2814	0.43988063509134473	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2572	0.4020515257480237	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	2477	0.3872012555512655	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2306	0.3604707691971006	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATG	2011	0.31435677227032494	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1987	0.31060512506272286	No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGT	1904	0.29763067846976565	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1888	0.2951295803313642	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1817	0.28403095734220807	No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACAC	1786	0.27918507969905537	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1432	0.22384828338692458	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1422	0.2222850970504237	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1418	0.22165982251582333	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	1387	0.21681394487267067	No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGAC	1370	0.21415652810061916	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1361	0.21274966039776838	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1333	0.20837273865556594	No Hit
TGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATG	1247	0.19492933616165847	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	1218	0.19039609578580594	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1179	0.18429966907345255	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	1170	0.18289280137060177	No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG	1133	0.17710901192554857	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1102	0.17226313428239587	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	1076	0.1681988498074936	No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAA	1069	0.167104619371943	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1066	0.16663566347099273	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAAC	1019	0.15928868768943866	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	1005	0.15710022681833744	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	966	0.15100380010598405	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	950	0.14850270196758264	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	931	0.14553264792823098	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	887	0.13865462804762715	No Hit
AAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGA	865	0.13521561810732524	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	854	0.1334961131371743	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	845	0.13208924543432352	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC	840	0.13130765226607308	No Hit
TTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAG	792	0.12380435785086889	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTA	786	0.12286644604896838	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	771	0.12052166654421706	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	753	0.1177079311385155	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTT	740	0.11567578890106438	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	726	0.11348732802996316	No Hit
TTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAG	699	0.10926672492141079	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	689	0.10770353858490994	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGG	676	0.1056713963474588	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	671	0.10488980317920836	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	663	0.10363925411000768	No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGA	655	0.102388705040807	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	645	0.10082551870430612	No Hit
CCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC	644	0.10066920007065602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04314394288742401	0.0	0.0	0.0	0.0
2	0.08222360129994576	0.0	0.0	0.0	0.0
3	0.1094230435550609	0.0	0.0	0.0	0.0
4	0.1431878684234797	0.0	0.0	0.0	0.0
5	0.1660103889363924	0.0	0.0	0.0	0.0
6	0.20743482685366543	0.0	0.0	0.0	0.0
7	0.23150789643577882	0.0	0.0	0.0	0.0
8	0.2663669517397482	0.0	0.0	0.0	0.0
9	0.2980996343707159	0.0	0.0	0.0	0.0
10	0.3184210567452272	0.0	0.0	0.0	0.0
11	0.33811720458513816	0.0	0.0	0.0	0.0
12	0.3700062058497559	0.0	0.0	0.0	0.0
13	0.39532982450107	0.0	0.0	0.0	0.0
14	0.4170581145784321	0.0	0.0	0.0	0.0
15	0.4447265127344975	0.0	0.0	0.0	0.0
16	0.4775534258010158	0.0	0.0	0.0	0.0
17	0.5131940742732356	0.0	0.0	0.0	0.0
18	0.567749277417116	0.0	0.0	0.0	0.0
19	0.5910407538309789	0.0	0.0	0.0	0.0
20	0.618240196086094	0.0	0.0	0.0	0.0
21	0.6477844178459605	0.0	0.0	0.0	0.0
22	0.6735769923982249	0.0	0.0	0.0	0.0
23	0.7029648955244412	0.0	0.0	0.0	0.0
24	0.7325091172843077	0.0	0.0	0.0	0.0
25	0.7586143291038722	0.0	0.0	0.0	0.0
26	0.79050333036849	0.0	0.0	0.0	0.0
27	0.819422277593756	0.0	0.0	0.0	0.0
28	0.8502170484228232	0.0	0.0	0.0	0.0
29	0.8874208832315439	0.0	0.0	0.0	0.0
30	0.9247810366739146	0.0	0.0	0.0	0.0
31	0.9543252584337811	0.0	0.0	0.0	0.0
32	0.9840257988272976	0.0	0.0	0.0	0.0
33	1.0143516137554145	0.0	0.0	0.0	0.0
34	1.0410821001095794	0.0	0.0	0.0	0.0
35	1.0631230274542416	0.0	0.0	0.0	0.0
36	1.0804743957894012	0.0	0.0	0.0	0.0
37	1.1059543330743655	0.0	0.0	0.0	0.0
38	1.119554054201923	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	10	0.00338585	185.59703	44
GGGTCCA	10	0.00338585	185.59703	44
TTGAGGA	35	1.8189894E-12	185.59703	44
TTAAGCA	35	1.8189894E-12	185.59703	44
TGGGGTC	10	0.00338585	185.59703	44
CGTACTG	20	6.213322E-7	185.59703	44
TCGTAGG	80	0.0	185.59703	44
CTACAGC	10	0.00338585	185.59703	44
GTCCAGT	10	0.00338585	185.59703	44
CAGTAGT	10	0.00338585	185.59703	44
TCAAGGT	10	0.00338585	185.59703	44
CTATACG	15	4.5904773E-5	185.59702	44
ATGACCA	15	4.5904773E-5	185.59702	44
AGCGACG	60	0.0	170.1306	44
CTGGCAA	50	0.0	167.03732	44
TCGGGCG	45	0.0	164.97513	44
GGCATAT	30	2.5131158E-8	154.66418	44
TGTATCA	25	1.8876799E-6	148.47762	44
TGTATAT	25	1.8876799E-6	148.47762	44
TGAAAGC	25	1.8876799E-6	148.47762	44
>>END_MODULE