FastQCFastQC Report
Tue 24 May 2016
ERR840974.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840974.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792782
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG518930.5299107036182363No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC492720.5031460927037894No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA389010.3972415601613515No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA315890.3225743205556909No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTATACG270570.27629533670819995No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA250040.25533091617887543No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239780.2448538117156085No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT216040.2206114666904665No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG210810.21527079843092597No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA203870.20818394609417426No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT186250.19019110197694586No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185690.1896192522206662No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTATA179090.18287959437879858No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG165490.16899181458343504No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA151160.15435858778434974No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150300.15348038994434882No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG145640.14872178304387865No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA142280.14529068450620058No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137830.14074652126433532No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135690.1385612382671237No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132650.1354569110187483No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG130990.13376178495549068No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT128860.13158671356106977No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA126200.1288704272187413No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG121730.12430584077129461No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA119040.12155891962059402No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA109950.11227657268384No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC106100.10834510560941724No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100620.10274914728010896No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTA100500.10262660804662045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100380.10250406881313195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG43550.0137.8318544
CTATGCG6750.0135.6791544
CTATCAT51600.0100.9325144
CGTGCGC14800.095.4304744
TCGTGCG27000.083.1535443
CCTTAGC83300.076.8402843
GAACACA44900.074.21632444
CAATGAA111350.067.1005943
AACGCTT11150.064.93671443
TACGGGC2100.063.0522844
CCAGAAT51750.061.62065544
ACCTTAG76150.057.6857642
ACGACGC1153.9616498E-757.56947344
CCCTATC51650.056.33407642
CCTATCA52150.055.53551543
ACTATAC65550.055.22839743
AACCTTA107200.054.45958341
CTCGTGC34600.053.7267742
CACTATA73900.052.9346442
GCAATGA111250.052.19943242
Produced by FastQC (version 0.11.5)