##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840974.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9792782
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.628994702424706	35.0	33.0	39.0	22.0	39.0
2	34.84980856308248	35.0	33.0	39.0	27.0	39.0
3	35.52632745219898	35.0	35.0	39.0	32.0	39.0
4	35.648558499515254	35.0	35.0	39.0	33.0	39.0
5	36.44646485544149	35.0	35.0	40.0	33.0	41.0
6	36.521010372741884	35.0	35.0	40.0	34.0	41.0
7	36.53272185575049	35.0	35.0	40.0	33.0	41.0
8	36.470928383783075	35.0	35.0	40.0	33.0	41.0
9	36.4747024900585	35.0	35.0	40.0	33.0	41.0
10	36.5040096879518	35.0	35.0	40.0	33.0	41.0
11	36.4394507097166	35.0	35.0	40.0	33.0	41.0
12	36.38268635000759	35.0	35.0	40.0	33.0	41.0
13	36.33240472421422	35.0	35.0	40.0	33.0	41.0
14	36.36194055989401	35.0	35.0	40.0	33.0	41.0
15	36.3928438313035	35.0	35.0	40.0	33.0	41.0
16	36.37081188981844	35.0	35.0	40.0	33.0	41.0
17	36.36701307146427	35.0	35.0	40.0	33.0	41.0
18	36.350319755918186	35.0	35.0	40.0	33.0	41.0
19	36.296150164478284	35.0	35.0	40.0	33.0	41.0
20	36.26563534243895	35.0	35.0	40.0	33.0	41.0
21	36.18079663164155	35.0	35.0	40.0	33.0	41.0
22	36.109369839949466	35.0	35.0	40.0	33.0	41.0
23	36.038566262375696	35.0	35.0	40.0	33.0	41.0
24	35.96004189616393	35.0	35.0	40.0	33.0	41.0
25	35.88230263066715	35.0	35.0	39.0	33.0	41.0
26	35.81541697342185	35.0	34.0	39.0	33.0	41.0
27	35.723204103601795	35.0	34.0	39.0	32.0	41.0
28	35.614369586875966	35.0	34.0	39.0	32.0	41.0
29	35.619905744252684	35.0	34.0	39.0	32.0	41.0
30	35.62227383088556	35.0	34.0	39.0	32.0	41.0
31	35.53091521744948	35.0	34.0	39.0	32.0	41.0
32	35.53044480896835	35.0	34.0	39.0	32.0	41.0
33	35.40597738796334	35.0	34.0	38.0	31.0	41.0
34	35.34245629508764	35.0	34.0	38.0	31.0	41.0
35	35.2977942888895	35.0	34.0	38.0	31.0	41.0
36	35.27686297584482	35.0	34.0	38.0	31.0	41.0
37	35.20925754371407	35.0	34.0	38.0	31.0	41.0
38	35.127914422710774	35.0	34.0	38.0	31.0	41.0
39	35.04797776961284	35.0	34.0	38.0	31.0	41.0
40	34.833141746470694	35.0	34.0	37.0	30.0	40.0
41	34.75405334259355	35.0	34.0	37.0	30.0	40.0
42	34.67219080539552	35.0	33.0	37.0	30.0	40.0
43	34.58653248994887	35.0	33.0	37.0	30.0	40.0
44	34.39574591226152	35.0	33.0	36.0	29.0	40.0
45	34.25347604299159	35.0	33.0	36.0	29.0	40.0
46	34.03805200769572	35.0	32.0	36.0	28.0	40.0
47	34.02298748644011	35.0	33.0	36.0	29.0	39.0
48	34.78020330456507	35.0	34.0	37.0	31.0	39.0
49	35.32474043557092	35.0	34.0	37.0	32.0	40.0
50	35.6815291027628	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	76.0
21	335.0
22	821.0
23	2076.0
24	5019.0
25	11001.0
26	19193.0
27	36114.0
28	65892.0
29	116329.0
30	202712.0
31	376114.0
32	711348.0
33	1389437.0
34	2497387.0
35	673390.0
36	450747.0
37	755308.0
38	768197.0
39	829497.0
40	881779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.05613869480603	20.998711091495757	25.94515021369821
2	27.883588136650033	24.707391627833644	28.97860893870608	18.43041129681024
3	28.181399320438256	21.583835931403353	22.902531680987078	27.332233067171313
4	20.93736999353197	26.335049631453046	27.30985944545687	25.41772092955812
5	19.141445199127276	31.321926700706705	26.591034090210524	22.945594009955496
6	23.737963328500523	35.47935612168228	23.56628586238313	17.21639468743407
7	26.618697322170554	32.649108292209505	22.04299043928477	18.689203946335166
8	24.884236164963134	29.091008050623408	25.326265814964533	20.69848996944893
9	21.97582872773028	31.3550122937486	27.526304578208727	19.14285440031239
10	26.63346329980592	28.051150326842773	25.45740321800281	19.8579831553485
11	23.691357573363728	28.412855509292456	27.41897042127559	20.476816496068224
12	22.729955593824105	28.563752363730753	28.02588682153856	20.680405220906582
13	20.133216485366468	29.050345448310804	29.675264904293797	21.14117316202893
14	16.619781794386927	31.374179472186757	28.585901330183805	23.42013740324251
15	18.040603783480528	33.718365220424594	26.76834836106839	21.47268263502649
16	18.63977978882814	38.638090789726554	23.523100994181227	19.199028427264082
17	20.422582673646776	37.888334489627155	21.2149519921918	20.47413084453427
18	21.28416623590722	31.000363328827298	24.971739389276713	22.743731045988767
19	21.529397876926087	30.3019203327512	26.067699658789508	22.100982131533208
20	22.20743809062634	28.900296156904137	25.363742397206433	23.52852335526309
21	21.955425945354445	30.795692174093126	25.98029855050383	21.268583330048603
22	22.433451495193093	33.581008951286776	22.455386017987532	21.5301535355326
23	23.36085904904245	30.637105982753422	24.08601559801903	21.916019370185104
24	23.419534918677858	29.751657904771083	24.27121322623132	22.557593950319735
25	20.16258610149081	31.232885572215917	26.3961723431155	22.208355983177775
26	21.69081596154771	32.69402355260575	26.95494119897983	18.66021928686671
27	23.465226618549785	30.829987118664327	22.989428506927037	22.71535775585885
28	21.788192197167785	31.086739845186013	24.08322943085941	23.041838526786794
29	24.243370320899388	32.11599619182266	23.142285712366753	20.498347774911196
30	23.30124822522721	33.04947513968295	23.79196288394357	19.857313751146275
31	20.677482886781554	32.68491173866717	24.54213430641711	22.095471068134167
32	22.355558595239895	32.780919539293436	25.10996999074016	19.753551874726508
33	22.723761620625645	30.363240405717246	24.982527807748937	21.930470165908172
34	19.180974665939406	34.18728868534014	24.558547386165166	22.07318926255529
35	21.560286968134687	32.76688589570751	24.012315775775303	21.660511360382504
36	20.68946415171002	33.45811146136743	24.614397191104477	21.238027195818074
37	19.690951736451552	33.930488969616576	24.180472003191344	22.198087290740524
38	22.8847308862696	32.19419752140709	23.70340148742219	21.21767010490112
39	22.009632095004132	33.648525304754855	24.662402870005828	19.679439730235185
40	19.92594433057353	33.53266043316053	25.800536006721703	20.740859229544238
41	20.635945851475345	34.676407245891404	23.955149134925424	20.732497767707827
42	21.836703464102143	34.30788695485967	22.180763431776146	21.67464614926204
43	19.623157527482807	32.29470796987069	24.83043682061819	23.251697682028315
44	20.16393633800331	32.219805834300445	23.369674530620756	24.246583297075492
45	20.668168134629216	31.37823399031598	26.601459814884187	21.352138060170617
46	19.395438161728695	33.3452991472667	27.004487252163774	20.25477543884083
47	22.842887349992726	31.316131402184826	25.701699529605293	20.13928171821715
48	21.16614848123032	33.909901479436535	23.154084933465487	21.76986510586766
49	18.916495789614423	34.634779196859114	22.834484801001643	23.61424021252482
50	22.073672787648857	33.63261073443286	24.22016269100326	20.073553786915017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302434.0
1	240212.5
2	162713.5
3	135778.0
4	108073.5
5	82006.5
6	58831.0
7	44387.0
8	41118.0
9	38206.0
10	36088.5
11	33980.5
12	31971.5
13	31732.5
14	35308.0
15	39804.0
16	42321.0
17	43190.0
18	44864.0
19	51286.0
20	59761.5
21	69407.0
22	78975.5
23	89206.5
24	99895.5
25	111345.0
26	128195.5
27	149337.5
28	171585.0
29	193469.0
30	217929.0
31	249549.0
32	294982.5
33	342217.0
34	400020.5
35	448700.5
36	473492.5
37	502219.0
38	526287.5
39	560540.5
40	602500.0
41	646879.0
42	710456.0
43	768535.5
44	779853.5
45	751452.0
46	723304.5
47	692105.5
48	683091.5
49	718956.0
50	775587.0
51	783534.5
52	732683.5
53	664915.5
54	578462.5
55	537875.0
56	519568.5
57	476936.5
58	406992.0
59	338582.5
60	316280.0
61	295916.0
62	263037.0
63	221767.0
64	172679.0
65	130733.0
66	98031.5
67	73777.5
68	55452.5
69	42003.5
70	33701.5
71	26368.5
72	21031.0
73	15802.0
74	9946.5
75	6097.0
76	4645.5
77	3378.5
78	2304.0
79	1798.0
80	1305.5
81	951.5
82	657.0
83	419.0
84	279.5
85	184.5
86	121.0
87	84.0
88	32.0
89	14.5
90	43.5
91	73.5
92	74.0
93	74.0
94	38.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	52097.0
25	50547.0
26	51386.0
27	59256.0
28	50528.0
29	46064.0
30	104335.0
31	50115.0
32	56578.0
33	51917.0
34	52530.0
35	51248.0
36	54166.0
37	54485.0
38	58698.0
39	90361.0
40	98417.0
41	101389.0
42	123056.0
43	217868.0
44	483186.0
45	884199.0
46	1086557.0
47	1042166.0
48	1620501.0
49	1436437.0
50	1764695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.08263245104839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43386424775147	7.805926295648676
2	12.644441805321465	2.802674021540972
3	4.3245836724092905	1.4378331403535167
4	2.1116325994024185	0.9360979188331566
5	1.1525354552473497	0.6386563418654054
6	0.7813537499977273	0.5195673855283899
7	0.5993321928983409	0.46495248869812034
8	0.4487222537807	0.3978419049006046
9	0.3612090983514593	0.36028329075034315
>10	3.49246673040899	8.733581999822674
>50	1.673090168049075	13.863825725275692
>100	1.8616072086990545	34.2405121635788
>500	0.06306425730243713	4.803372935616645
>1k	0.04265215469730047	9.403198669810815
>5k	0.0047222028414868385	3.5230362220162013
>10k+	0.0047222028414868385	10.068639495760033
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	51893	0.5299107036182363	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	49272	0.5031460927037894	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	38901	0.3972415601613515	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	31589	0.3225743205556909	No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTATACG	27057	0.27629533670819995	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	25004	0.25533091617887543	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23978	0.2448538117156085	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	21604	0.2206114666904665	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	21081	0.21527079843092597	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	20387	0.20818394609417426	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	18625	0.19019110197694586	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18569	0.1896192522206662	No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTATA	17909	0.18287959437879858	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	16549	0.16899181458343504	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA	15116	0.15435858778434974	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15030	0.15348038994434882	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	14564	0.14872178304387865	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	14228	0.14529068450620058	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13783	0.14074652126433532	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13569	0.1385612382671237	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13265	0.1354569110187483	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	13099	0.13376178495549068	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	12886	0.13158671356106977	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	12620	0.1288704272187413	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	12173	0.12430584077129461	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	11904	0.12155891962059402	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	10995	0.11227657268384	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	10610	0.10834510560941724	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10062	0.10274914728010896	No Hit
AAGCAGTGTGGATTCTTTACTGTGTTCTGACAGCCATGTGTCACTA	10050	0.10262660804662045	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10038	0.10250406881313195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03271797534143005	0.0	0.0	0.0	0.0
2	0.05905369893866728	0.0	0.0	0.0	0.0
3	0.08081462448566709	0.0	0.0	0.0	0.0
4	0.1061598226122056	1.0211602790708503E-5	0.0	0.0	0.0
5	0.13372093854432784	1.0211602790708503E-5	0.0	0.0	0.0
6	0.17582337685041902	1.0211602790708503E-5	0.0	0.0	0.0
7	0.2027105269983545	1.0211602790708503E-5	0.0	0.0	0.0
8	0.2292504826514059	1.0211602790708503E-5	0.0	0.0	0.0
9	0.25466716199747935	1.0211602790708503E-5	0.0	0.0	0.0
10	0.27769432629052704	1.0211602790708503E-5	0.0	0.0	0.0
11	0.30112995469520304	1.0211602790708503E-5	0.0	0.0	0.0
12	0.3236771736570874	1.0211602790708503E-5	0.0	0.0	0.0
13	0.3458261401101342	1.0211602790708503E-5	0.0	0.0	0.0
14	0.36983361827108985	1.0211602790708503E-5	0.0	0.0	0.0
15	0.3925442228776256	1.0211602790708503E-5	0.0	0.0	0.0
16	0.41738905246741936	1.0211602790708503E-5	0.0	0.0	0.0
17	0.44391879651768007	1.0211602790708503E-5	0.0	0.0	0.0
18	0.46947843830282343	1.0211602790708503E-5	0.0	0.0	0.0
19	0.49544654419959516	1.0211602790708503E-5	0.0	0.0	0.0
20	0.5210776672042735	1.0211602790708503E-5	0.0	0.0	0.0
21	0.545268954215462	1.0211602790708503E-5	0.0	0.0	0.0
22	0.5706447871503726	1.0211602790708503E-5	0.0	0.0	0.0
23	0.5959797736741204	1.0211602790708503E-5	0.0	0.0	0.0
24	0.619885135807169	1.0211602790708503E-5	0.0	0.0	0.0
25	0.6456490096481265	1.0211602790708503E-5	0.0	0.0	0.0
26	0.6715149995169912	1.0211602790708503E-5	0.0	0.0	0.0
27	0.6960841158314358	1.0211602790708503E-5	0.0	0.0	0.0
28	0.72410475388914	1.0211602790708503E-5	0.0	0.0	0.0
29	0.753177187034287	1.0211602790708503E-5	0.0	0.0	0.0
30	0.7829746439775745	1.0211602790708503E-5	0.0	0.0	0.0
31	0.8133847970883045	1.0211602790708503E-5	0.0	0.0	0.0
32	0.8435396601292666	1.0211602790708503E-5	0.0	0.0	0.0
33	0.8724895540409252	2.0423205581417006E-5	0.0	0.0	0.0
34	0.9010207722381648	2.0423205581417006E-5	0.0	0.0	0.0
35	0.9277139019330768	2.0423205581417006E-5	0.0	0.0	0.0
36	0.94995477281124	2.0423205581417006E-5	0.0	0.0	0.0
37	0.9695814733749817	2.0423205581417006E-5	0.0	0.0	0.0
38	0.9831424818810426	2.0423205581417006E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	4355	0.0	137.83185	44
CTATGCG	675	0.0	135.67915	44
CTATCAT	5160	0.0	100.93251	44
CGTGCGC	1480	0.0	95.43047	44
TCGTGCG	2700	0.0	83.15354	43
CCTTAGC	8330	0.0	76.84028	43
GAACACA	4490	0.0	74.216324	44
CAATGAA	11135	0.0	67.10059	43
AACGCTT	1115	0.0	64.936714	43
TACGGGC	210	0.0	63.05228	44
CCAGAAT	5175	0.0	61.620655	44
ACCTTAG	7615	0.0	57.68576	42
ACGACGC	115	3.9616498E-7	57.569473	44
CCCTATC	5165	0.0	56.334076	42
CCTATCA	5215	0.0	55.535515	43
ACTATAC	6555	0.0	55.228397	43
AACCTTA	10720	0.0	54.459583	41
CTCGTGC	3460	0.0	53.72677	42
CACTATA	7390	0.0	52.93464	42
GCAATGA	11125	0.0	52.199432	42
>>END_MODULE