FastQCFastQC Report
Tue 24 May 2016
ERR840973.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840973.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165805
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCGTGGAGAAAGAAATGGCTCC28311.7074273996562226No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC24831.497542293658213No Hit
AATTCGTGGAGAAAGAAATGGCTC19231.1597961460752089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA18971.1441150749374265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA18831.1356714212478514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17981.0844063809897169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA16330.9848918910768674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA15040.9070896535086397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13900.838334187750671No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC13340.8045595729923706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12460.7514851783721842No Hit
AAAAAAAAAAAAAAAAAAAAAAAA10650.6423207985283919No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10170.6133711287355629No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG9010.5434094267362263No Hit
TCAGAACTGCGTCTCGCGACCCAGGCGCGGG8800.5307439462018637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.4939537408401436No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCCC7630.46017912608184314No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT7600.4583697717197913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.3823768885136154No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.37091764422062057No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.3455866831518953No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA5160.31120895027291096No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5140.31000271403154306No Hit
ACACTTCCGGAAGAATTGCACTGGACTGTGGGTATCCTGGTCTCCGCGTG5140.31000271403154306No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4970.2997497059799162No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG4800.28949669792828925No Hit
ACTTTCCTGTCCGCGACTCCGCCCCCACCAGAGGGGCTAGGCTCTGGGAT4800.28949669792828925No Hit
CTCACACCTGGGAGGTTGCAAGGTGGGAGGATCCTTCAAGCCTAGGAAT4660.28105304423871413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.2792436898766623No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTACC4600.27743433551461055No Hit
TGAGTCTATGGATGTGTGTGCATGTCACACGTGTGTGCATATGTGACAT4250.25632520129067277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.253912728807937No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGAG4100.24727842948041373No Hit
TGGGCCTATTTTAAAAATAGATAGCAAATTTGGATGCCAGGTGTCCAAAA3980.2400410120322065No Hit
CTACGTTGGGGTGGTGGTATGTGCCTGTAATCCCAGCTACTTGGGTGGC3960.23883477579083864No Hit
ACCTGTATCCTGTAGAGTCGAAAATGGGAATCTGAATGTCTAGAGTGGAA3940.23762853954947077No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG3890.2346129489460511No Hit
AGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAG3850.23220047646331535No Hit
CTTTCTCTCTCGCGCGCGGTGTGGTGGCAGCAGGCGCAGCCCAGCCTCGA3830.23099424022194748No Hit
CCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAAG3810.22978800398057958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.21772564156690086No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA3420.20626639727390608No Hit
CGCCGCCGTCGCTCTCCAACGCCAGCGCCGCCTCTCGCTCGCCGAGCTC3360.20264768854980247No Hit
AAGCAGTGGTATCAACGCAGAGTG3320.20023521606706676No Hit
AGACGGAGAGGCCCCGCCGAGGTGAGCGAGGCTCCAACCGGTCACTGGTG3270.19721962546364707No Hit
CCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGAAGG3270.19721962546364707No Hit
CGAATAAGGGGACATAGAGGATGTCTTGGCATCTCGTGCCGATGAAGGA3270.19721962546364707No Hit
ACACTTCCGGAAGAATTGCACTGGACTGTGGGTATCCTGGTCTCCGCG3140.1893790898947559No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG3120.188172853653388No Hit
CTCAATACTGTAGCTGCCGTGCATGGGGAAACACAGGCCTTGGGGATGAA3090.18636349929133622No Hit
AACTGCTTCCTGCAGGCTGGCCATGGCGCTTCACGTTCCCAAGGCTCCG3080.18576038117065227No Hit
TGGAATTCGTGGAGAAAGAAATGGCTCC3040.18334790868791653No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3020.18214167244654864No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.18093543620518077No Hit
CCTTGCCTTGGGCTTCGGGCCGCTGCGTTCCGCCTTGCCAGGCGA2980.17972919996381292No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCT2970.17912608184312898No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.17852296372244505No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTA2960.17852296372244505No Hit
AGTGCAGTGCTATGCTTGGGGGGTTTCTGCTGGGTGTAGGTCCTTGGCTG2930.17671360936039324No Hit
AGTGCAGTGCTAAAGTTGGGAACACAAAGCACCCAACTTACACTTAGGAG2930.17671360936039324No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.1761104912397093No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2890.1743011368776575No Hit
CTCACACCTGGGAGGTTGCAAGGTGGGAGGATCCTTCAAGCCTAGGA2880.17369801875697355No Hit
AGAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTAAGCCGAGATCACAC2880.17369801875697355No Hit
TGAGTCTATGGATGTGTGTGCATGTCACACGTGTGTGCATATGTGAC2860.17249178251560568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2810.169476191912186No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2810.169476191912186No Hit
TGCGCATCATCGTTTCGACGCGCTCGGAAGCAACTTCCTTTGCCTGCT2800.16887307379150207No Hit
CAGTGCTATGCTTGGGGCCCGAGGATGGTCGGGCCTGGCGTTGGGCGTGC2760.16646060130876633No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGTC2710.16344501070534664No Hit
AGTGCAGTGCTAAAGTTGGGACCCTAACCTATGTGCTCCTGTAAGGCAAG2700.1628418925846627No Hit
TGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGGTGC2680.16163565634329483No Hit
CTTTTCCGCCCGCTCCCCCCTCCCCCCGAGCGCCGCTCCGGCTGCACCGC2630.15862006573987517No Hit
CAGGTGCTGGGGGCTTCCGAGACAATC2630.15862006573987517No Hit
AGCCCGTCCTCCAGTATTCCGTCTGTAGCAGGAGCTAGGGCTGCTGCCT2610.1574138294985073No Hit
TGCTAAAGTTGGGGGCTCACGCCTGTAATCCCAGCAATTTGGGAGGCTGA2550.15379512077440366No Hit
TCCCAGCATTTTGGGAGGCCAAGGCGGGCAGATCACTTGAGGTCAGGAGT2540.15319200265371974No Hit
CGGAGACCTCTCTGGGAAAGCCCAGAATGCATCCAAAGGGATCTATGC2510.15138264829166792No Hit
AGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGA2490.15017641205030005No Hit
AAGCTAGGGTCGCCGCCACTGCCGCAGGAGGCGTGAGGAAGTAGCTGAT2490.15017641205030005No Hit
ACTTTCCTGTCCGCGACTCCGCCCCCACCAGAGGGGCTAGGCTCTGGG2480.1495732939296161No Hit
TGTTGCCATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGACGG2470.14897017580893218No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG2470.14897017580893218No Hit
AATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG2470.14897017580893218No Hit
CTTTCTCTCTCGCGCGCGGTGTGGTGGCAGCAGGCGCAGCCCAGCCTC2450.1477639395675643No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAGT2440.14716082144688036No Hit
AGCCTGCGCCGGCGTCCTGCCGGCTTCGTCTCCTCAGCGGCCATGAGGC2410.14535146708482857No Hit
AATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGT2400.14474834896414462No Hit
TAATCTGCTGTGGTCTTTTTCCACGGTTTGGAAGCTTTATTTTTTTGC2380.14354211272277675No Hit
CATCTACACCACACAAGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGG2370.1429389946020928No Hit
AAGCAGTAGTGCTACATTGGGGGTAAGAGTCCAGTGCTACGTTGGGGGTA2330.14052652211935707No Hit
TGGGCCTATTTTAAAAATAGATAGCAAATTTGGATGCCAGGTGTCCAA2330.14052652211935707No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.13992340399867315No Hit
AGGTGTTACTGGTTGCGTCGGGTCACGTGGGCGCGCAGGCGCAGCGCGG2310.1393202858779892No Hit
TAGTCGCCGCCGCCGCCGCCGCCGCTGCAGCCACTGCAGGCACCGCTG2300.13871716775730528No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.13811404963662136No Hit
AAGCCGTAGCCGTCTTCGCCTCCGCCGGTAAAGTTGGAGCGGAAGTAC2280.13751093151593738No Hit
TCAAGTGAATAAGTGCATCAGGTGGATGCCTTGGCGATGATAGGCGACG2280.13751093151593738No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.13690781339525346No Hit
AGTAGCGTTGGGCTGCGGCAGCGGAGGAGCTCAACATGCGTGAGTGTATC2260.1363046952745695No Hit
AGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGG2250.1357015771538856No Hit
CGAATAAGGGGACATAGAGGATGTCTTGGCATCTCGTGCCGATGAAG2250.1357015771538856No Hit
CGATGCTCCCACCTCAGCCTCCCAAGTAGCTGGGACCACAGGCACATG2250.1357015771538856No Hit
ACCTGTATCCTGTAGAGTCGAAAATGGGAATCTGAATGTCTAGAGTGG2240.13509845903320164No Hit
AATTCCTTCGACTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGATGCT2230.13449534091251772No Hit
CCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA2200.13268598655046593No Hit
CGCCGCCGTCGCTCTCCAACGCCAGCGCCGCCTCTCGCTCGCCGAGC2190.13208286842978198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.13208286842978198No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2170.13087663218841408No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA2160.13027351406773016No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAG2160.13027351406773016No Hit
CTAGACAGGTGCTGGGGGCTTCCGAGACAATC2140.1290672778263623No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA2110.1272579234643105No Hit
ACACATTTCTGGTGTTTTAAGATGCCTGAGCCAGCCAAGTCTGCTCCCG2100.12665480534362655No Hit
TGCTGGGGGCTTCCGAGACAATCGCG2100.12665480534362655No Hit
AGGATTGAAGAAGAAGAAGAAGATGGAAATAAGTCGTCAAAAGATATTG2090.12605168722294263No Hit
AGTTGGTGTTGGGGGCTTAGGCGAGGGACGTTACCGGGAAGTTGCAGGCG2030.122432978498839No Hit
TCTACACCACACAAGCAGGTGCTGGGGGCTTCCGAGACAATC2000.12062362413678719No Hit
CTACGTTGGGGTGGTGGTATGTGCCTGTAATCCCAGCTACTTGGGTG1940.11700491541268357No Hit
AATTCCTTCGATTCTGTTTCGCTACCCGTTGTAGCGCCGGAAGATGC1930.11640179729199963No Hit
AGCTGGAGAGAACAGGACGCCTCCGTAATTTTACCGGGCCTTTTGTGTTT1930.11640179729199963No Hit
TGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.1157986791713157No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAGC1910.11519556105063176No Hit
CTCATTATCTCTTGCTGCCACCATGTAGGAAGTGCCTTTTGCTCTCCGCC1900.11459244292994783No Hit
AGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA1900.11459244292994783No Hit
AGTGCAGTGCTAAAGTTGGGAACACAAAGCACCCAACTTACACTTAGG1880.11338620668857996No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAG1870.11278308856789603No Hit
ACTAGGCGGCGGCGGCTGGCGTGGGGCTGCTTAGATGCGCCACGG1850.11157685232652814No Hit
ATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGCTCCGAAGCGGGGAAG1840.11097373420584421No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG1840.11097373420584421No Hit
ACACTTCCGGAAGAATTGCACTGGACTGTGGGTATCCTGGTCTCCG1840.11097373420584421No Hit
AGAAGGAGGAGAAGCTGAGCCAGGCCTATGCAATCAGTGCTGGTGTCTCT1820.10976749796447634No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGC1810.1091643798437924No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGG1790.10795814360242453No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC1790.10795814360242453No Hit
CGACACACTTTTCTACAGAAAACGCAAGTGGTAGTACATCTCAAGCCACA1780.1073550254817406No Hit
AGCTCTTTCTGGCCTTCACCACATCTCACCTCCCCTTAGCGGAGCAGAAA1770.10675190736105666No Hit
AACTGCTTCCTGCAGGCTGGCCATGGCGCTTCACGTTCCCAAGGCTC1770.10675190736105666No Hit
AGAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTAAGCCGAGATCAC1760.10614878924037274No Hit
AGACGGAGAGGCCCCGCCGAGGTGAGCGAGGCTCCAACCGGTCACTGG1750.10554567111968878No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1750.10554567111968878No Hit
AGTGCAGTACTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.10554567111968878No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG1750.10554567111968878No Hit
AGAGTCAGTAAGAGCATGGCTACGTCCTCGATGTCTAAGGTATGTGGTGG1740.10494255299900485No Hit
CCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGAA1730.10433943487832091No Hit
TGAGTCTATGGATGTGTGTGCATGTCACACGTGTGTGCATATGTG1720.10373631675763698No Hit
TCGCGCGAGGTTTTGGTTTGTGAGGATCGGCGAG1720.10373631675763698No Hit
ACTCGCGGAACTTTGGTGCAGCCTGATGCGCAACGTGGGGACTCAGGCGC1720.10373631675763698No Hit
CACCACACAAGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCC1720.10373631675763698No Hit
AGGTGTTACTGGTTGCGTCGGGTCACGTGGGCGCGCAGGCGCAGCG1720.10373631675763698No Hit
CCCGGACGCGGAGCGAGAGGCTGAGAGAGTCGGAGACACTATCCGCTTC1710.10313319863695304No Hit
ACTTTCGCTTTTTCCCGTCCTGTTACCCGGCTGTTTTTGATCTCGGTGC1700.1025300805162691No Hit
TGCGCATCATCGTTTCGACGCGCTCGGAAGCAACTTCCTTTGCCTG1700.1025300805162691No Hit
CTCAATACTGTAGCTGCCGTGCATGGGGAAACACAGGCCTTGGGGATG1700.1025300805162691No Hit
CTTTCTCTCTCGCGCGCGGTGTGGTGGCAGCAGGCGCAGCCCAGCC1690.10192696239558517No Hit
AGTGCAGTGCTATGCTTGGGGGGCCGGGCGCGGTGGCTCACACCTGTAAT1690.10192696239558517No Hit
AGTGCAGTACTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1680.10132384427490124No Hit
CTACGTTGGGAGAGTGCAGTACTACGTTGGGAAAAAAAAAAAAAAAAAA1680.10132384427490124No Hit
AAGCAGTAGTGCTACATTGGGGGTAAGAGTCCAGTGCTACGTTGGGGG1680.10132384427490124No Hit
AGTGCAGTGCTAAAGTTGGGACCCTAACCTATGTGCTCCTGTAAGGCA1670.10072072615421732No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA1670.10072072615421732No Hit
AGAGGTTGCATTTTCCCAAGCTTGAACGCTTCATGTGCCTACTCTGCAGG1670.10072072615421732No Hit
ATTTTTAGTGTGTATGTGGTGAACAAAGCTGGCGGCTTGATTTACCAGT1670.10072072615421732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACA100.0014520326245.8600944
AGCCTAA100.0014520326245.8600944
GGATGAA550.0245.8600944
GTGTATC400.0245.8600944
GGCGTGC100.0014520326245.8600944
TGTCTCT151.4803461E-5245.8600944
TATGTTC201.5042133E-7245.8600944
AGCTAAG600.0245.8600944
GAAAAGC201.5042133E-7245.8600944
AGTGGAA450.0245.8600944
GTCTGCG100.0014520326245.8600944
AGGTCTC151.4803461E-5245.8600944
ATCACAC301.6370905E-11245.8600944
TCCCGGG100.0014520326245.8600944
ACTGGTG301.6370905E-11245.8600944
TTCAGGG151.4803461E-5245.8600944
TGTGTTT151.4803461E-5245.8600944
CTCGCCA100.0014520326245.8600944
CCGCGTG650.0226.9477544
GCCTCGA400.0215.1275844
Produced by FastQC (version 0.11.5)