FastQCFastQC Report
Tue 24 May 2016
ERR840972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855533
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA68360.7990340524561882No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59900.7001483285858057No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT34250.40033522961709256No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC33710.39402337490196165No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33450.39098433374282465No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC32410.3788281691062764No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC32390.37859439670941974No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25970.3035534573184202No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA25710.30051441615928315No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG25500.2980598059922878No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA25240.2950207648331508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24550.28695561714159473No Hit
AATTCGTGGAGAAAGAAATGGCTCC24170.28251394160131754No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23430.2738643629176198No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA22860.26720184960720395No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21990.25703275034393763No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC20980.24522724430267448No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20950.24487658570738943No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG19380.22652545255413878No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA19180.22418772858557182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA18880.22068114263272134No Hit
AATTCGTGGAGAAAGAAATGGCTC18860.22044737023586466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA18340.21436928791759058No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA17570.20536905063860775No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA17450.20396641625746756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA16900.19753767534390843No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC16160.18888809666021064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15470.18082294896865464No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15200.17766702161108924No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG14910.1742773218566671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA14730.17217337028495688No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC14730.17217337028495688No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT14720.17205648408652852No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT14600.17065384970538833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13220.1545235543222763No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC13170.15393912333013454No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13130.15347157853642115No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG12680.14821169960714548No Hit
ATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAAA12640.14774415481343212No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC12570.14692595142443365No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA12080.14119852770144461No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11590.13547110397845555No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT11570.13523733158159884No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA11470.13406846959731536No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC11310.13219829042246178No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC11280.13184763182717676No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA11100.12974368025546648No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACT11050.12915924926332473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10940.1278735010806129No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT10710.1251851185167609No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.12273050834976558No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC10470.12237984975448053No Hit
AAAAAAAAAAAAAAAAAAAAAAAA10280.12015901198434192No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGG9190.10741841635565198No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC9040.10566512337922675No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA9020.10543135098237005No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA9010.10531446478394171No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC9000.10519757858551335No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.1046131475933716No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG8920.10426248899808657No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC8900.10402871660122988No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG8770.10250919602166136No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8770.10250919602166136No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC8720.10192476502951962No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT8640.10098967544209282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGGC207.709277E-7177.7672144
CGGATAC100.0038530284177.7672144
GTCAATC207.709277E-7177.7672144
ATTGCAA100.0038530284177.7672144
TGCAGCA207.709277E-7177.7672144
AAGGATA100.0038530284177.7672144
GTAGTAC100.0038530284177.7672144
CCATTAA100.0038530284177.7672144
GCACAGT155.4544354E-5177.767244
ACGTGTT155.4544354E-5177.767244
CATCGTT500.0177.767244
ATCATCA301.5643309E-10177.767244
ATCGCCC251.0955773E-8177.767244
GTTATCC251.0955773E-8177.767244
GCTTTAG155.4544354E-5177.767244
CTATGCG500.0159.9904844
GTACAAG450.0158.0152944
ACTTGCA407.2759576E-12155.5463144
CTGTTCG407.2759576E-12155.5463144
CGAGCAA354.5838533E-10152.3718944
Produced by FastQC (version 0.11.5)