FastQCFastQC Report
Tue 24 May 2016
ERR840971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852601
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG148320.3849866622575242No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105190.27303632013800544No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC92290.2395524478138276No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC82510.2141670004238695No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67250.17455739641867923No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA60880.15802311217798054No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA58150.15093699036053823No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA57620.14956129638132784No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA55460.14395469450379106No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG48920.1269791499301381No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46960.12189167785607696No Hit
CCGGGCGCGTTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG46930.12181380838555562No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45070.1169859012132323No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42550.11044486568943943No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA41480.10766752124084483No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC40070.10400765612634166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG151.6806971E-5238.8692544
CGATAGT355.820766E-11204.7450744
TATCGAG401.4915713E-10179.1519544
CGACGTA550.0173.723144
CTATCAT28800.0155.0991444
TAAGCCA16550.0130.6203544
ACGTTCT355.9699232E-9105.44105543
ACCACTC2750.0104.2338644
ACTTCTA11900.0103.3761944
CCCATAC2700.0101.7406144
TTAAGCC11950.093.6763843
TCGCAAT401.5095793E-892.2609243
TGTCACG404.1836756E-1179.88724542
ACGTAGA1351.8189894E-1279.62308544
TCACGGG1850.077.4711144
TTCAACG800.076.884143
AATGTCC1400.074.68741643
TACGGGC801.9383533E-674.64664544
CGAGATT2400.074.64664544
CCTATGC3050.074.401944
Produced by FastQC (version 0.11.5)