##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840971.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3852601
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.26921085261619	39.0	35.0	39.0	31.0	39.0
2	36.832509777160936	39.0	35.0	39.0	33.0	39.0
3	37.013343452903634	39.0	35.0	39.0	33.0	39.0
4	37.565023214186986	39.0	35.0	40.0	34.0	41.0
5	38.251565111466256	40.0	35.0	41.0	35.0	41.0
6	38.28809004617919	40.0	35.0	41.0	35.0	41.0
7	38.288411647092445	40.0	35.0	41.0	35.0	41.0
8	38.244670548546296	40.0	35.0	41.0	35.0	41.0
9	38.13483591994084	40.0	35.0	41.0	34.0	41.0
10	38.05897911566757	40.0	35.0	41.0	34.0	41.0
11	37.85605361157307	40.0	35.0	41.0	34.0	41.0
12	37.896353658216874	40.0	35.0	41.0	34.0	41.0
13	37.80805668689802	40.0	35.0	41.0	34.0	41.0
14	37.804381767019215	40.0	35.0	41.0	34.0	41.0
15	37.83562922815002	39.0	35.0	41.0	34.0	41.0
16	37.77026948806793	39.0	35.0	41.0	34.0	41.0
17	37.776500083969246	39.0	35.0	41.0	34.0	41.0
18	37.79971998138401	39.0	35.0	41.0	34.0	41.0
19	37.86769535698091	40.0	35.0	41.0	34.0	41.0
20	37.855471147933564	39.0	35.0	41.0	34.0	41.0
21	37.85655924400165	40.0	35.0	41.0	34.0	41.0
22	37.71429535526778	39.0	35.0	41.0	33.0	41.0
23	37.64534193912113	39.0	35.0	41.0	33.0	41.0
24	37.477309225637434	39.0	35.0	41.0	33.0	41.0
25	37.415377572480175	39.0	35.0	41.0	33.0	41.0
26	37.37561723359012	39.0	35.0	41.0	33.0	41.0
27	37.31783970981649	38.0	35.0	41.0	33.0	41.0
28	37.245092856751086	38.0	35.0	41.0	33.0	41.0
29	37.11551788650905	38.0	35.0	41.0	33.0	41.0
30	37.03695856148294	38.0	35.0	41.0	33.0	41.0
31	36.90422696972629	37.0	35.0	41.0	33.0	41.0
32	36.829431909619124	37.0	35.0	41.0	33.0	41.0
33	36.74560986303915	37.0	35.0	40.0	33.0	41.0
34	36.65988237706292	37.0	35.0	40.0	33.0	41.0
35	36.58038792203739	36.0	35.0	40.0	33.0	41.0
36	36.51040470669458	36.0	35.0	40.0	33.0	41.0
37	36.44766768320795	36.0	35.0	40.0	33.0	41.0
38	36.395643692834	35.0	35.0	40.0	33.0	41.0
39	36.29210891663014	35.0	35.0	40.0	33.0	41.0
40	36.24975239226184	35.0	35.0	40.0	33.0	41.0
41	36.158294801898144	35.0	35.0	40.0	33.0	41.0
42	36.105346374243595	35.0	35.0	40.0	33.0	41.0
43	36.012031520141754	35.0	35.0	40.0	32.0	41.0
44	35.883648102286436	35.0	35.0	39.0	32.0	41.0
45	35.75310679810823	35.0	35.0	39.0	32.0	41.0
46	35.53084104982515	35.0	34.0	39.0	31.0	41.0
47	35.392179124494454	35.0	34.0	39.0	31.0	41.0
48	35.38396887232409	35.0	34.0	39.0	31.0	41.0
49	35.591499617327784	35.0	34.0	39.0	31.0	41.0
50	35.92886260425495	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	26.0
21	72.0
22	188.0
23	519.0
24	1132.0
25	2397.0
26	4544.0
27	8200.0
28	14671.0
29	25492.0
30	43548.0
31	74894.0
32	125790.0
33	237648.0
34	621715.0
35	222813.0
36	273452.0
37	430957.0
38	489039.0
39	592616.0
40	682884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.080791392620206	18.47816578981317	38.441042817566625
2	27.29145841990904	18.912937000224	29.929753950642695	23.865850629224255
3	25.98670352834358	17.193034004819083	27.669540655780338	29.150721811056997
4	21.88251521504563	19.446732220647817	32.07233243203747	26.598420132269084
5	23.44969022226802	21.023407303273814	29.7880834272742	25.73881904718397
6	23.235678960785194	23.219508067406927	27.600314696486866	25.94449827532101
7	29.904887633056216	20.72874922682105	22.854793423975128	26.51156971614761
8	27.746631431596473	20.0850023140211	23.21244790207966	28.955918352302767
9	27.314507783183362	21.695161268971276	25.88578469454792	25.10454625329745
10	30.31383213574414	18.843555301989486	24.257430239985922	26.585182322280453
11	28.191395890724213	20.803140527659107	24.31097847921443	26.69448510240225
12	29.44403015002073	20.396765717498386	23.898244328961134	26.26095980351975
13	28.840775361891875	20.13662977297675	25.743335476474204	25.27925938865717
14	24.09782378190734	22.33825926951688	22.770824178262945	30.793092770312835
15	25.72511402037221	23.06758473041979	24.194693403235892	27.01260784597211
16	27.074903422389184	25.19017671438075	20.561926864474156	27.17299299875591
17	27.219896376499932	27.135174392572708	19.57903245106358	26.065896779863785
18	26.04360015480451	22.686050281355374	19.49534872674331	31.775000837096805
19	28.112514117086096	21.834054447891177	22.16502565409706	27.88840578092567
20	28.108724469520723	20.65469536035525	23.252472809927628	27.984107360196397
21	28.702583008206663	25.75039564180147	18.452884168383903	27.09413718160796
22	28.099899262861634	23.397180242646463	23.66995699788273	24.832963496609175
23	30.57056778005301	22.459657774059654	21.785645593717078	25.18412885217026
24	29.436321072439114	22.316170296378992	23.973933454307883	24.27357517687401
25	28.787071479889185	25.212580276440637	21.19125479619379	24.809093447476382
26	29.553064156033816	25.268787115139602	22.801297881995104	22.37685084683148
27	28.2641818322874	24.63319367046988	22.276191536736576	24.826432960506143
28	29.12558667252776	22.076586913913715	22.347702289245024	26.4501241243135
29	29.423863326164966	24.678389049048917	21.006624632982017	24.891122991804096
30	30.24479221244573	24.664653241629498	20.232268383536297	24.858286162388474
31	26.464998198554586	25.245970731160476	22.5025189246085	25.786512145676436
32	26.778495069089143	23.1053622977806	25.05853220002993	25.057610433100326
33	25.826361447165507	21.931821441401326	25.03440070918595	27.207416402247215
34	27.0307107013346	24.82419499088337	24.3085570989054	23.836537208876635
35	25.484211378998513	22.100254156198933	22.723991565541915	29.691542899260643
36	26.045883490520545	22.58857084569805	26.206947922129864	25.15859774165154
37	25.882320396601994	24.261582507218897	25.43314774050087	24.42294935567824
38	25.991368838219785	24.38509642999083	23.129182342771998	26.49435238901739
39	26.804999081051278	24.763321222060426	21.680403771931065	26.751275924957234
40	28.16276053552087	24.60249822913903	22.312251059317514	24.922490176022585
41	26.94981870821141	23.488040247809412	22.186851556379395	27.37528948759978
42	25.87568926044555	26.516083876054108	22.070442540404475	25.537784323095863
43	27.360725210288916	23.465957035177276	22.587571478035677	26.585746276498128
44	26.855060843443525	24.534167152041515	22.24160401122344	26.369167993291516
45	26.942342561279865	21.952497352810756	24.496134551382298	26.60902553452708
46	25.570325051244918	24.690006511367102	22.69768999490277	27.04197844248521
47	26.966283187695144	23.4930952829991	23.286016638377234	26.254604890928523
48	24.639986098885206	24.170771449440938	21.103057620953226	30.08618483072063
49	25.266806457804464	24.34461861674233	21.83913953762917	28.549435387824033
50	22.958477749905875	28.807910593681875	25.31433361274099	22.919278043671262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68360.0
1	49919.5
2	34418.0
3	32583.0
4	23515.5
5	15340.0
6	9085.5
7	7108.0
8	8197.5
9	9294.0
10	9403.5
11	8975.0
12	8415.0
13	7812.0
14	8576.5
15	9937.5
16	10502.0
17	10411.0
18	10237.0
19	11778.5
20	13983.5
21	16252.0
22	17982.0
23	19168.5
24	21805.0
25	24049.5
26	25735.5
27	28941.5
28	32274.0
29	35667.5
30	40409.5
31	50226.5
32	65094.5
33	72908.5
34	77965.0
35	85199.5
36	88345.0
37	91573.5
38	95242.5
39	101476.5
40	112835.0
41	125926.0
42	138284.0
43	142819.0
44	137963.0
45	134471.5
46	138235.0
47	144295.5
48	156361.0
49	178689.5
50	199994.0
51	215000.5
52	228033.5
53	230545.0
54	228798.5
55	227278.5
56	232582.0
57	245034.5
58	248625.0
59	244302.0
60	241056.5
61	243539.5
62	242423.5
63	232686.5
64	217322.5
65	206196.0
66	204408.0
67	206016.5
68	204496.5
69	199698.5
70	182856.5
71	158804.5
72	146125.0
73	137478.5
74	119025.5
75	99725.5
76	83431.0
77	62456.0
78	46873.0
79	37838.5
80	30042.5
81	24272.5
82	20014.0
83	16513.5
84	12651.0
85	9821.0
86	7465.5
87	5450.0
88	3284.5
89	2011.0
90	1490.5
91	931.5
92	574.0
93	395.0
94	288.0
95	114.5
96	40.0
97	26.0
98	13.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	27693.0
25	19015.0
26	16291.0
27	15563.0
28	19114.0
29	23638.0
30	20378.0
31	22341.0
32	28033.0
33	22129.0
34	21339.0
35	19128.0
36	19137.0
37	18447.0
38	23705.0
39	19999.0
40	29455.0
41	28235.0
42	42352.0
43	70398.0
44	177756.0
45	325734.0
46	456671.0
47	463902.0
48	676957.0
49	599778.0
50	645413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.289114372469033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55667682129824	9.907922055094431
2	10.173593459830878	2.703960941333909
3	3.4907658862406645	1.3916756132929624
4	1.7371329114124472	0.9233983175976049
5	1.0195806895964599	0.6774662198004101
6	0.72096882802016	0.5748622328726918
7	0.558565764400672	0.5195991029366285
8	0.40552982952783434	0.43113058288346073
9	0.3291357807831828	0.393653073140965
>10	3.457947771088305	10.819294327699362
>50	1.7874924507048517	17.4308214127762
>100	1.6741766939208171	37.73828098231539
>500	0.05447210773551035	4.921978431432267
>1k	0.030934777232512048	8.314412407958256
>5k	0.00235373305029983	2.118751968268655
>10k+	6.724951572285228E-4	1.132792330596817
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	14832	0.3849866622575242	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10519	0.27303632013800544	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	9229	0.2395524478138276	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	8251	0.2141670004238695	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6725	0.17455739641867923	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA	6088	0.15802311217798054	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5815	0.15093699036053823	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5762	0.14956129638132784	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	5546	0.14395469450379106	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG	4892	0.1269791499301381	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4696	0.12189167785607696	No Hit
CCGGGCGCGTTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	4693	0.12181380838555562	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4507	0.1169859012132323	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4255	0.11044486568943943	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4148	0.10766752124084483	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	4007	0.10400765612634166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.019675019551726222	0.0	0.0	0.0	0.0
2	0.03587186942016575	0.0	0.0	0.0	0.0
3	0.04508642343185811	0.0	0.0	0.0	0.0
4	0.05549497599154441	0.0	0.0	0.0	0.0
5	0.06478739947375811	0.0	0.0	0.0	0.0
6	0.08051703251906958	0.0	0.0	0.0	0.0
7	0.09217149660709739	0.0	0.0	0.0	0.0
8	0.10356639579338738	0.0	0.0	0.0	0.0
9	0.11397494835307367	0.0	0.0	0.0	0.0
10	0.12267037256129042	0.0	0.0	0.0	0.0
11	0.13172918763194008	0.0	0.0	0.0	0.0
12	0.1419560447604099	0.0	0.0	0.0	0.0
13	0.1528058576530505	0.0	0.0	0.0	0.0
14	0.1634999316046484	0.0	0.0	0.0	0.0
15	0.17533609112389267	0.0	0.0	0.0	0.0
16	0.18686077276105156	0.0	0.0	0.0	0.0
17	0.19963136592655195	0.0	0.0	0.0	0.0
18	0.22283646814191244	0.0	0.0	0.0	0.0
19	0.23501006203341587	0.0	0.0	0.0	0.0
20	0.2477027857283949	0.0	0.0	0.0	0.0
21	0.2604993353840691	0.0	0.0	0.0	0.0
22	0.27236145139348716	0.0	0.0	0.0	0.0
23	0.2848984361474235	0.0	0.0	0.0	0.0
24	0.2974094644111861	0.0	0.0	0.0	0.0
25	0.31064727439981454	0.0	0.0	0.0	0.0
26	0.328401513678681	0.0	0.0	0.0	0.0
27	0.33927728306149535	0.0	0.0	0.0	0.0
28	0.35391674351950797	0.0	0.0	0.0	0.0
29	0.3696204200746457	0.0	0.0	0.0	0.0
30	0.38324757741588084	0.0	0.0	0.0	0.0
31	0.39627773548311906	0.0	0.0	0.0	0.0
32	0.40935980653070486	0.0	0.0	0.0	0.0
33	0.4245183967921931	0.0	0.0	0.0	0.0
34	0.4389502053288155	0.0	0.0	0.0	0.0
35	0.44878771510467863	0.0	0.0	0.0	0.0
36	0.4587290508412369	0.0	0.0	0.0	0.0
37	0.46488073901242305	0.0	0.0	0.0	0.0
38	0.47069499281135	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	15	1.6806971E-5	238.86925	44
CGATAGT	35	5.820766E-11	204.74507	44
TATCGAG	40	1.4915713E-10	179.15195	44
CGACGTA	55	0.0	173.7231	44
CTATCAT	2880	0.0	155.09914	44
TAAGCCA	1655	0.0	130.62035	44
ACGTTCT	35	5.9699232E-9	105.441055	43
ACCACTC	275	0.0	104.23386	44
ACTTCTA	1190	0.0	103.37619	44
CCCATAC	270	0.0	101.74061	44
TTAAGCC	1195	0.0	93.67638	43
TCGCAAT	40	1.5095793E-8	92.26092	43
TGTCACG	40	4.1836756E-11	79.887245	42
ACGTAGA	135	1.8189894E-12	79.623085	44
TCACGGG	185	0.0	77.47111	44
TTCAACG	80	0.0	76.8841	43
AATGTCC	140	0.0	74.687416	43
TACGGGC	80	1.9383533E-6	74.646645	44
CGAGATT	240	0.0	74.646645	44
CCTATGC	305	0.0	74.4019	44
>>END_MODULE