FastQCFastQC Report
Tue 24 May 2016
ERR840970.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840970.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11378851
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA754420.6630019146924413No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA263050.23117448325845905No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223480.19639944314236998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA209200.18384984564786022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA208650.18336649280318373No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA203970.17925359950666372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA185710.16320628506340404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA179260.15753787443037967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA155780.13690310207946302No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA135190.1188081292214829No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130970.11509949466778326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124540.10944866050183802No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT121580.1068473433741245No Hit
AAAAAAAAAAAAAAAAAAAAAAAA116290.10219836783169056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114770.10086255633367552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA232600.0221.5857144
CTATCAT62000.0175.390744
TTAAGCC224650.0114.8762243
ACTTCTA17300.0105.47883644
ACGACAC5450.097.94134544
AACGACA8750.095.1859643
GTGTTAG22300.084.8585743
GATTAGC751.1175143E-782.7566444
ATTAAGC315650.080.2478642
CGACGGG52150.078.07529444
ACCACTC9200.075.5604244
ACGACAA2950.071.535444
CTAATCG5250.070.67589642
AAACTAC20600.066.8894143
TGTGTTA37050.064.9849442
AATCGCA10550.062.3616444
AATATCG609.44591E-662.0674844
AATGTAC1409.615178E-962.0674844
GATTAAG345100.061.0858541
TGCACCG17450.058.9597943
Produced by FastQC (version 0.11.5)