##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840970.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11378851
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.16982558256541	39.0	37.0	39.0	34.0	39.0
2	37.69412491647883	39.0	37.0	39.0	35.0	39.0
3	37.659359104007954	39.0	37.0	39.0	35.0	39.0
4	38.28977152438326	39.0	38.0	40.0	35.0	41.0
5	38.993415591785144	40.0	38.0	41.0	35.0	41.0
6	38.8747838424108	40.0	38.0	41.0	35.0	41.0
7	38.971904632550334	40.0	38.0	41.0	35.0	41.0
8	38.7186966416908	40.0	38.0	41.0	35.0	41.0
9	38.71311092833538	40.0	38.0	41.0	35.0	41.0
10	38.64408269341079	40.0	38.0	41.0	35.0	41.0
11	38.57221823187596	40.0	38.0	41.0	35.0	41.0
12	38.613665826189305	40.0	38.0	41.0	35.0	41.0
13	38.44474174062039	40.0	38.0	41.0	34.0	41.0
14	38.50112115889381	40.0	38.0	41.0	35.0	41.0
15	38.475926875217894	40.0	38.0	41.0	35.0	41.0
16	38.3379528389993	40.0	37.0	41.0	34.0	41.0
17	38.32968275970922	40.0	37.0	41.0	34.0	41.0
18	38.47107076101093	40.0	37.0	41.0	35.0	41.0
19	38.45364193625525	40.0	37.0	41.0	35.0	41.0
20	38.54551738132435	40.0	37.0	41.0	35.0	41.0
21	38.409646457274114	40.0	37.0	41.0	35.0	41.0
22	38.42059439920604	40.0	37.0	41.0	34.0	41.0
23	38.31782374160625	40.0	37.0	41.0	34.0	41.0
24	38.1903257191785	40.0	36.0	41.0	34.0	41.0
25	38.14457559439493	40.0	36.0	41.0	34.0	41.0
26	38.09587877612658	40.0	36.0	41.0	34.0	41.0
27	37.999319381353615	40.0	36.0	41.0	33.0	41.0
28	37.92262017288465	40.0	35.0	41.0	33.0	41.0
29	37.80688316925675	40.0	35.0	41.0	33.0	41.0
30	37.68822552909847	39.0	35.0	41.0	33.0	41.0
31	37.51530014315131	39.0	35.0	41.0	33.0	41.0
32	37.36666573777862	39.0	35.0	41.0	33.0	41.0
33	37.21478161816104	38.0	35.0	41.0	33.0	41.0
34	37.056346500788926	38.0	35.0	40.0	33.0	41.0
35	36.9056205373768	38.0	35.0	40.0	33.0	41.0
36	36.80865266149481	37.0	35.0	40.0	33.0	41.0
37	36.72088721445448	37.0	35.0	40.0	33.0	41.0
38	36.60531178960277	37.0	35.0	40.0	33.0	41.0
39	36.528812423301616	36.0	35.0	40.0	33.0	41.0
40	36.40546753600279	36.0	35.0	40.0	33.0	41.0
41	36.31042367429682	36.0	35.0	40.0	33.0	41.0
42	36.21175071408602	35.0	35.0	40.0	33.0	41.0
43	36.09450565978684	35.0	35.0	40.0	32.0	41.0
44	35.93195636107456	35.0	35.0	40.0	32.0	41.0
45	35.71639563914862	35.0	34.0	39.0	31.0	41.0
46	35.45598416947054	35.0	34.0	39.0	30.0	41.0
47	35.27691521843315	35.0	34.0	39.0	30.0	41.0
48	35.26981072509702	35.0	34.0	39.0	30.0	41.0
49	35.331946423435994	35.0	34.0	39.0	30.0	41.0
50	35.37744227991239	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	61.0
21	170.0
22	340.0
23	819.0
24	1832.0
25	3785.0
26	6868.0
27	13025.0
28	25548.0
29	48809.0
30	87381.0
31	155034.0
32	255492.0
33	435198.0
34	772951.0
35	730095.0
36	1188127.0
37	1812678.0
38	1975455.0
39	2062458.0
40	1802714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.525787533381006	23.59341905434916	33.88079341226983
2	30.70014714139415	24.64373599759765	24.848106368560412	19.808010492447785
3	23.74560489455394	19.05202027867313	27.964950063938794	29.23742476283414
4	22.961747192225296	23.581185833262076	27.518402341326027	25.938664633186605
5	23.251741322564115	20.04562675089075	29.442146663138484	27.260485263406647
6	25.84905980401712	27.541612066104037	24.810659705448295	21.798668424430552
7	36.53096433022983	19.11040051407651	19.42524776886524	24.933387386828425
8	23.553388650576405	20.343231491474842	29.59998333750921	26.503396520439544
9	28.993147023368177	23.448334106844353	25.98762388223556	21.570894987551906
10	27.736622968347156	18.064460111130728	24.730423133231994	29.46849378729012
11	25.142169451028053	25.618799297046774	25.7520289175067	23.487002334418474
12	26.022311040016255	20.27438446992583	29.891040844106314	23.8122636459516
13	24.135573969638937	24.091263696132412	23.724328581154637	28.048833753074014
14	26.020720369745593	20.40889717248253	25.298309996325642	28.27207246144624
15	23.56791560061732	22.49441529729144	29.96088972427884	23.9767793778124
16	34.41593531719503	22.35661579539094	18.35707313506434	24.870375752349688
17	26.270148014065743	21.910876590263815	24.675575767711518	27.14339962795892
18	30.99141556559621	21.853709131088895	18.625984293141723	28.52889101017317
19	29.79415935756607	27.239033185336552	19.929683585803172	23.03712387129421
20	33.56214085235847	21.822247255017224	21.552553944154816	23.06305794846949
21	27.544143077363437	28.528697669035303	21.390138600109974	22.53702065349129
22	27.672899486951714	30.670100170922353	18.54396370951689	23.113036632609038
23	31.389715886076726	27.990638070574963	18.75918754890103	21.860458494447286
24	28.151392438480826	28.19930588773858	19.10228897451948	24.54701269926111
25	26.514751225349748	32.76960130056854	18.826599304830673	21.88904816925104
26	28.1895266796642	29.306629400501784	21.49655245794323	21.00729146189079
27	25.760397842181494	34.673022336022385	17.753590758868715	21.812989062927404
28	25.856537341729297	28.78178165978288	22.015257182837928	23.346423815649896
29	30.163724987849406	30.530220538390644	18.884718713187336	20.421335760572607
30	25.886856250781985	31.95400102695416	17.29195889032529	24.867183831938565
31	24.31730092223238	30.016861026455455	23.44606948248299	22.219768568829174
32	23.176128320310323	29.446921479235638	23.694837983346893	23.682112217107147
33	26.768757821757795	28.54236846922728	20.99322570813098	23.69564800088395
34	24.7354251820718	29.80701091332531	24.0145195503819	21.443044354220994
35	22.849057850060035	29.52676881120596	20.361396318711915	27.26277702002209
36	23.312837416770133	29.340116104293458	25.7514856596978	21.595560819238614
37	23.402642625622473	30.604412856507096	20.182145906096448	25.810798611773983
38	24.16041559774491	33.54337092363438	18.6966720551111	23.59954142350961
39	23.578328776624645	35.265238384527194	18.84349906587877	22.312933772969387
40	23.678499805294997	35.05901217636295	19.231719728271383	22.03076829007067
41	26.885828939841005	30.86505509965572	18.489342863314715	23.75977309718856
42	23.824139019066497	34.30184875789435	18.817980754411643	23.05603146862751
43	23.809253538139583	29.881096131544865	24.31610360015673	21.99354673015882
44	23.99260280725762	29.976988029180646	22.730173000598324	23.300236162963415
45	24.22505977496599	32.3925128695903	21.835927994269728	21.546499361173986
46	23.17252924824874	34.66537773928784	19.84559389898977	22.316499113473654
47	28.21662380284013	28.453327946323885	19.48629431154881	23.843753939287176
48	22.54599129446096	28.706441036214407	18.558511874667115	30.189055794657516
49	24.181893500324282	28.633713897712425	17.822340348612993	29.362052253350303
50	19.929291339017645	40.96677521550951	18.879378205712484	20.224555239760363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	361326.0
1	300909.0
2	182172.0
3	109358.5
4	83195.5
5	56906.0
6	31055.5
7	20232.0
8	20640.5
9	20763.0
10	20797.5
11	21426.5
12	22527.0
13	24353.0
14	29435.0
15	37461.5
16	47629.0
17	66725.5
18	99567.5
19	149079.0
20	209403.0
21	258040.0
22	267152.5
23	239467.5
24	207732.5
25	189769.5
26	170993.5
27	154508.5
28	149977.5
29	147465.5
30	144991.0
31	143653.0
32	147568.5
33	153769.5
34	168538.0
35	185516.0
36	190983.0
37	195101.0
38	206166.0
39	226978.0
40	290121.5
41	375991.5
42	397820.5
43	518240.0
44	648881.0
45	526007.5
46	422029.5
47	438072.0
48	474239.0
49	544898.0
50	623981.5
51	672074.5
52	676009.5
53	672237.5
54	670823.5
55	653229.0
56	635697.0
57	641667.0
58	646261.5
59	649437.0
60	665628.0
61	670799.0
62	643076.0
63	603555.5
64	568235.0
65	541839.0
66	502955.5
67	461880.0
68	420227.5
69	376438.5
70	350140.0
71	344921.0
72	343573.0
73	316503.5
74	273201.0
75	241699.5
76	213753.5
77	165732.0
78	135524.0
79	130090.0
80	113831.0
81	80868.0
82	55098.5
83	44459.0
84	38119.0
85	33310.5
86	26379.0
87	18926.5
88	12033.0
89	7767.0
90	5024.0
91	2769.0
92	1397.5
93	702.5
94	607.0
95	481.5
96	421.5
97	396.0
98	276.0
99	156.0
100	156.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	87789.0
25	69444.0
26	71468.0
27	73400.0
28	79345.0
29	79683.0
30	76745.0
31	75417.0
32	86215.0
33	71854.0
34	70527.0
35	58454.0
36	54559.0
37	72788.0
38	71448.0
39	74533.0
40	94774.0
41	98659.0
42	145123.0
43	202960.0
44	503895.0
45	1014497.0
46	1299175.0
47	1369356.0
48	1797821.0
49	1868677.0
50	1810245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.313823676038155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22653874706423	11.782910178501435
2	12.12542756062285	3.956241744411492
3	4.305675316366445	2.1072608375241595
4	2.138817748244118	1.39569182480142
5	1.3410755715790987	1.093903520549175
6	0.9220263150883248	0.9025064837410874
7	0.6714486903008494	0.7667726878752364
8	0.5068760275234071	0.6615268910902021
9	0.4035393962870966	0.5924943501626322
>10	3.342686100443401	11.983536736687086
>50	1.144253185673852	13.516337427626187
>100	0.7842257063051054	22.09157046704528
>500	0.046971013557655525	5.325167268506279
>1k	0.03406176148717404	11.06980766960264
>5k	0.0037327957794163332	4.259991723736641
>10k+	0.0026440636770865694	8.494280188139035
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	75442	0.6630019146924413	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	26305	0.23117448325845905	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22348	0.19639944314236998	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	20920	0.18384984564786022	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	20865	0.18336649280318373	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	20397	0.17925359950666372	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	18571	0.16320628506340404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17926	0.15753787443037967	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	15578	0.13690310207946302	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13519	0.1188081292214829	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13097	0.11509949466778326	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12454	0.10944866050183802	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	12158	0.1068473433741245	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	11629	0.10219836783169056	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11477	0.10086255633367552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05647318872529397	0.0	0.0	0.0	0.0
2	0.10575760241521749	0.0	0.0	0.0	0.0
3	0.12643631593383198	0.0	0.0	0.0	0.0
4	0.1571687686217176	0.0	0.0	0.0	0.0
5	0.1788757054644621	0.0	0.0	0.0	0.0
6	0.23468977667428811	0.0	0.0	0.0	0.0
7	0.2546390668091181	0.0	0.0	0.0	0.0
8	0.28165409670976443	0.0	0.0	0.0	0.0
9	0.30818577376573436	0.0	0.0	0.0	0.0
10	0.3286096285117012	0.0	0.0	0.0	0.0
11	0.3531551647877277	0.0	0.0	0.0	8.78823353957267E-6
12	0.3746248193249037	0.0	0.0	0.0	8.78823353957267E-6
13	0.3987660968581098	0.0	0.0	0.0	8.78823353957267E-6
14	0.4159470934279744	0.0	0.0	0.0	8.78823353957267E-6
15	0.4388843829662591	0.0	0.0	0.0	8.78823353957267E-6
16	0.4530598036655898	0.0	0.0	0.0	8.78823353957267E-6
17	0.47417792886118293	0.0	0.0	0.0	8.78823353957267E-6
18	0.49760735947768364	0.0	0.0	0.0	8.78823353957267E-6
19	0.5252199892590209	0.0	0.0	0.0	8.78823353957267E-6
20	0.5494403608940832	0.0	0.0	0.0	8.78823353957267E-6
21	0.5781163669337088	0.0	0.0	0.0	8.78823353957267E-6
22	0.6084709255793929	0.0	0.0	0.0	8.78823353957267E-6
23	0.635573837815435	0.0	0.0	0.0	8.78823353957267E-6
24	0.6611827503497497	0.0	0.0	0.0	8.78823353957267E-6
25	0.6900784622278646	0.0	0.0	0.0	8.78823353957267E-6
26	0.7258202080333067	0.0	0.0	0.0	8.78823353957267E-6
27	0.7575896722788619	0.0	0.0	0.0	8.78823353957267E-6
28	0.7876190662835817	0.0	0.0	0.0	8.78823353957267E-6
29	0.8186063777441149	0.0	0.0	0.0	8.78823353957267E-6
30	0.8510349595051381	0.0	0.0	0.0	8.78823353957267E-6
31	0.8816443769234696	0.0	0.0	0.0	8.78823353957267E-6
32	0.9109091946102467	0.0	0.0	0.0	8.78823353957267E-6
33	0.9381439303493824	0.0	0.0	0.0	1.757646707914534E-5
34	0.9609933375522713	0.0	0.0	0.0	1.757646707914534E-5
35	0.9790531574760931	0.0	0.0	0.0	1.757646707914534E-5
36	0.9945907543740576	0.0	0.0	0.0	1.757646707914534E-5
37	1.0047060111781059	0.0	0.0	0.0	1.757646707914534E-5
38	1.011938727381174	0.0	0.0	0.0	1.757646707914534E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	23260	0.0	221.58571	44
CTATCAT	6200	0.0	175.3907	44
TTAAGCC	22465	0.0	114.87622	43
ACTTCTA	1730	0.0	105.478836	44
ACGACAC	545	0.0	97.941345	44
AACGACA	875	0.0	95.18596	43
GTGTTAG	2230	0.0	84.85857	43
GATTAGC	75	1.1175143E-7	82.75664	44
ATTAAGC	31565	0.0	80.24786	42
CGACGGG	5215	0.0	78.075294	44
ACCACTC	920	0.0	75.56042	44
ACGACAA	295	0.0	71.5354	44
CTAATCG	525	0.0	70.675896	42
AAACTAC	2060	0.0	66.88941	43
TGTGTTA	3705	0.0	64.98494	42
AATCGCA	1055	0.0	62.36164	44
AATATCG	60	9.44591E-6	62.06748	44
AATGTAC	140	9.615178E-9	62.06748	44
GATTAAG	34510	0.0	61.08585	41
TGCACCG	1745	0.0	58.95979	43
>>END_MODULE