##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840969.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6845457
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.81034969615615	35.0	35.0	39.0	27.0	39.0
2	35.569764882023215	35.0	35.0	39.0	33.0	39.0
3	35.83660798687363	35.0	35.0	39.0	33.0	39.0
4	35.97641559942601	35.0	35.0	39.0	33.0	39.0
5	36.72545529100541	35.0	35.0	40.0	34.0	41.0
6	36.72878932699453	35.0	35.0	40.0	33.0	41.0
7	36.74642291960931	35.0	35.0	40.0	33.0	41.0
8	36.666167795663604	35.0	35.0	40.0	33.0	41.0
9	36.594901260792376	35.0	35.0	40.0	33.0	41.0
10	36.542463417709	35.0	35.0	40.0	33.0	41.0
11	35.97223326360826	35.0	35.0	40.0	31.0	41.0
12	36.26654086060288	35.0	35.0	40.0	33.0	41.0
13	36.285557852456016	35.0	35.0	40.0	33.0	41.0
14	36.22439130068307	35.0	35.0	40.0	33.0	41.0
15	36.262184540783764	35.0	35.0	40.0	33.0	41.0
16	36.25357342833357	35.0	35.0	40.0	33.0	41.0
17	36.28254505141147	35.0	35.0	40.0	33.0	41.0
18	36.31283711226292	35.0	35.0	40.0	33.0	41.0
19	36.389018877775435	35.0	35.0	40.0	33.0	41.0
20	36.434322208144756	35.0	35.0	40.0	33.0	41.0
21	36.43702458433381	35.0	35.0	40.0	33.0	41.0
22	36.33316022582568	35.0	35.0	40.0	33.0	41.0
23	36.3085031430334	35.0	35.0	40.0	33.0	41.0
24	36.17050972637765	35.0	35.0	40.0	33.0	41.0
25	36.15603695999454	35.0	35.0	40.0	33.0	41.0
26	36.15787252604473	35.0	35.0	40.0	33.0	41.0
27	36.13093457423301	35.0	35.0	40.0	33.0	41.0
28	36.0843468605793	35.0	35.0	40.0	33.0	41.0
29	36.03122462289862	35.0	35.0	40.0	33.0	41.0
30	35.98528877796221	35.0	35.0	40.0	33.0	41.0
31	35.8771399897898	35.0	35.0	40.0	33.0	41.0
32	35.8425538109191	35.0	35.0	40.0	33.0	41.0
33	35.79806066792619	35.0	35.0	39.0	33.0	41.0
34	35.76137893307182	35.0	35.0	39.0	33.0	41.0
35	35.66526429153877	35.0	35.0	39.0	33.0	41.0
36	35.65062479827057	35.0	35.0	39.0	33.0	41.0
37	35.63611381080033	35.0	35.0	39.0	33.0	41.0
38	35.59021553639634	35.0	35.0	39.0	33.0	41.0
39	35.56700534407802	35.0	35.0	38.0	33.0	41.0
40	35.52711927993972	35.0	35.0	38.0	33.0	41.0
41	35.45759587044352	35.0	35.0	38.0	33.0	41.0
42	35.34324591211314	35.0	34.0	37.0	32.0	41.0
43	35.29329369664853	35.0	34.0	37.0	32.0	41.0
44	35.17329369453786	35.0	34.0	37.0	32.0	40.0
45	35.01640944799764	35.0	34.0	37.0	31.0	40.0
46	34.82079350445348	35.0	34.0	37.0	31.0	40.0
47	34.71827923508233	35.0	34.0	36.0	31.0	40.0
48	34.489470870684336	35.0	34.0	37.0	30.0	40.0
49	34.809262664321615	35.0	34.0	37.0	31.0	40.0
50	34.920133042969034	35.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	26.0
21	132.0
22	442.0
23	1260.0
24	2796.0
25	6439.0
26	12548.0
27	23489.0
28	43545.0
29	75204.0
30	126161.0
31	212295.0
32	352817.0
33	704775.0
34	2079621.0
35	409177.0
36	327029.0
37	460420.0
38	544055.0
39	757406.0
40	705818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.07391851267198	18.023208676937127	39.902872810390896
2	26.69750171537123	19.366902750247352	28.07124491469306	25.86435061968836
3	29.24614090775824	17.284105356296884	26.762420682797366	26.707333053147508
4	26.215576257363093	19.482190889519867	29.886594861380328	24.41563799173671
5	25.537739847025552	21.290090639675334	27.866364510068504	25.30580500323061
6	24.033515950797735	22.94606481349602	24.988426630975844	28.031992604730405
7	32.828896595216364	20.571570312982757	21.137127879117493	25.462405212683393
8	27.151949095582662	20.64357427122835	22.630775417915853	29.573701215273136
9	30.02826546131252	20.910335131752344	24.036729761066354	25.024669645868787
10	32.95115285948038	19.01764922341927	22.46006365973813	25.571134257362218
11	26.737747384871458	20.52023699805579	27.075591885245938	25.666423731826814
12	32.37979582663363	20.48657963960624	22.303361192685895	24.830263341074232
13	31.538712462878664	19.09672356425583	24.88720621574279	24.477357757122718
14	23.586591808260575	21.05450373875696	22.193025242872753	33.16587921010971
15	24.43530066728927	21.953625594317515	27.45154925376056	26.159524484632655
16	26.263944101905835	24.19205905464018	20.317226446678433	29.226770396775553
17	26.724716845055045	28.821085283276194	18.47946747748178	25.974730394186977
18	24.19832598466399	21.364797120192268	20.7944188386546	33.64245805648915
19	31.073557251181334	20.157310169357576	21.243154401524983	27.52597817793611
20	26.474375633358004	21.091170392276222	21.83794303287567	30.596510941490102
21	25.39329368367956	24.204607522916294	19.600064685235772	30.80203410816838
22	28.53482243771307	22.8324127958148	25.2438515061887	23.388913260283424
23	32.13247851823479	23.22237945545491	20.77028604518296	23.87485598112734
24	28.109781421459516	22.506824599146558	26.512108687557312	22.871285291836614
25	27.6880932452302	27.646051781192497	20.96063494530048	23.705220028276823
26	29.097439998130337	28.187828827137785	21.112845182980575	21.601885991751303
27	26.542665829826902	23.928249655546356	25.33558141175859	24.193503102868156
28	26.970185768592348	23.70044002891435	20.91323201767459	28.41614218481871
29	27.72685507126621	24.465374067566096	20.322556408680224	27.485214452487465
30	27.901154556947365	24.13842499662234	20.796968187257534	27.16345225917276
31	25.489236426913976	27.995136119621723	21.591138210210357	24.924489243253944
32	28.854829468604713	22.219449051827755	23.81493171000017	25.11078976956736
33	24.213670172951492	21.892390866615656	22.76365511349355	31.1302838469393
34	25.818569205866076	28.128414685520564	23.04588517389669	23.007130934716667
35	24.541621819203048	22.489011657901628	21.659760390782402	31.309606132112922
36	25.649240371407338	22.23105244589898	27.541024572834022	24.57868260985966
37	24.190669120155402	24.635541954845838	27.199925616319682	23.973863308679082
38	24.575116207600452	23.506167364844018	26.278915296764065	25.63980113079147
39	30.00134340346026	23.891223700812933	21.41817037341217	24.689262522314632
40	29.2630653009617	23.994383708604197	22.28045696624478	24.462094024189327
41	29.367918797388814	23.768316710754267	21.22368002365624	25.64008446820068
42	24.02510035838698	29.535385090415602	22.13051720293092	24.3089973482665
43	30.17138210860927	22.697900234739095	21.919732115494124	25.21098554115751
44	30.065879851227983	23.92967510273071	21.910807049405477	24.09363799663583
45	25.741210293040105	23.278183088685534	22.401290523774783	28.579316094499575
46	25.180053122768285	24.53326391402099	22.101315632438645	28.18536733077208
47	29.847357823430503	23.3745960794625	21.597714749644226	25.18033134746277
48	23.3270808569207	28.308692096631482	20.87945419120736	27.484772855240465
49	26.646909259014663	25.088895299932567	21.860032047494247	26.404163393558523
50	24.205310738598932	27.711490130053924	22.985902691359833	25.09729643998731
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105067.0
1	75095.5
2	50074.0
3	48359.5
4	37371.5
5	27673.5
6	20188.5
7	19621.0
8	21654.5
9	22470.5
10	21962.5
11	20102.5
12	18406.5
13	17401.0
14	16861.5
15	17221.5
16	18522.5
17	18434.5
18	17685.0
19	19597.0
20	23129.5
21	28365.0
22	33852.0
23	37499.5
24	39763.0
25	42322.0
26	48498.0
27	57130.0
28	62514.5
29	68686.5
30	78748.0
31	88191.5
32	99994.5
33	116037.5
34	136142.0
35	147748.0
36	150147.5
37	155149.5
38	161042.5
39	178895.5
40	201557.0
41	220329.5
42	240969.5
43	252939.5
44	249891.0
45	239381.5
46	238931.0
47	257244.0
48	286872.0
49	316497.5
50	347077.0
51	365995.0
52	365042.5
53	366610.0
54	384984.5
55	393009.0
56	392580.0
57	400334.0
58	406462.0
59	403620.5
60	401995.0
61	415172.5
62	418583.0
63	411419.5
64	399579.0
65	387031.0
66	384543.0
67	398166.0
68	464854.0
69	504599.0
70	421177.5
71	308975.0
72	254559.5
73	226776.5
74	192525.5
75	161512.5
76	131751.0
77	97712.0
78	74096.5
79	62286.5
80	52802.5
81	41609.5
82	30808.5
83	23420.0
84	18661.5
85	15692.0
86	11856.5
87	7997.5
88	4811.0
89	2852.5
90	1919.0
91	1469.0
92	1003.5
93	694.5
94	678.0
95	441.5
96	198.0
97	143.0
98	104.0
99	65.0
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	49785.0
25	35091.0
26	28906.0
27	31888.0
28	31620.0
29	36359.0
30	40363.0
31	44586.0
32	45961.0
33	37089.0
34	40183.0
35	28922.0
36	27716.0
37	34786.0
38	34764.0
39	33518.0
40	41460.0
41	43106.0
42	64480.0
43	108637.0
44	291968.0
45	536075.0
46	842564.0
47	755182.0
48	1278864.0
49	990128.0
50	1311456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.39729621674618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2567497672313	15.17969356194768
2	9.85522671784732	3.8232950385855133
3	3.171732963257544	1.845691314261741
4	1.496457154324093	1.1610889079237392
5	0.8465885424800568	0.8210764366094534
6	0.5532493168978044	0.6438924528947514
7	0.43507532457541737	0.5907499463170451
8	0.3228754289278651	0.5010328268818222
9	0.2572428530782508	0.4490834238719773
>10	2.7392631382416024	12.81741030577868
>50	1.2320532645909252	17.184075990185217
>100	0.7746512561599042	25.346127130848757
>500	0.03642121624018849	4.7128550113775445
>1k	0.018911016124713256	6.964239927206993
>5k	0.0023346933487300315	2.8763871933239615
>10k+	0.0011673466743650157	5.083300531985118
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	41332	0.6037873001028273	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	28743	0.41988431159526673	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	18025	0.26331331859947404	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13751	0.20087775001727423	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10396	0.15186714342081178	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7975	0.11650062223749269	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7525	0.10992691941531442	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	7291	0.10650859394778173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01747144127850047	0.0	0.0	0.0	0.0
2	0.03161220646043062	0.0	0.0	0.0	0.0
3	0.04097608092491122	0.0	0.0	0.0	0.0
4	0.0522098086365892	0.0	0.0	0.0	0.0
5	0.06354579394772329	0.0	0.0	0.0	0.0
6	0.08038908140099339	0.0	0.0	0.0	0.0
7	0.09156437619869645	0.0	0.0	0.0	0.0
8	0.10301722733778038	0.0	0.0	0.0	0.0
9	0.11322837905489729	0.0	0.0	0.0	0.0
10	0.12278216048979637	0.0	0.0	0.0	0.0
11	0.1327595805510136	0.0	0.0	0.0	0.0
12	0.1425617018703061	0.0	0.0	0.0	0.0
13	0.15229078204712995	0.0	0.0	0.0	0.0
14	0.16264801604918416	0.0	0.0	0.0	0.0
15	0.1731221158791882	0.0	0.0	0.0	0.0
16	0.1841659366204477	0.0	0.0	0.0	0.0
17	0.19601320992886231	0.0	0.0	0.0	0.0
18	0.21330935246543803	0.0	0.0	0.0	0.0
19	0.22337442189761764	0.0	0.0	0.0	0.0
20	0.233454099558291	0.0	0.0	0.0	0.0
21	0.2447024354984627	0.0	0.0	0.0	0.0
22	0.25434386630432415	0.0	0.0	0.0	0.0
23	0.2657967174434081	0.0	0.0	0.0	0.0
24	0.2769720122411112	0.0	0.0	0.0	0.0
25	0.2884102551517013	0.0	0.0	0.0	0.0
26	0.30073960000040906	0.0	0.0	0.0	0.0
27	0.3118564618841372	0.0	0.0	0.0	0.0
28	0.32561741312523035	0.0	0.0	0.0	0.0
29	0.3384726541997123	0.0	0.0	0.0	0.0
30	0.3510941636182946	0.0	0.0	0.0	0.0
31	0.36508884651528745	0.0	0.0	0.0	0.0
32	0.37718445970809544	0.0	0.0	0.0	0.0
33	0.3889002589600665	0.0	0.0	0.0	0.0
34	0.4009520474673933	0.0	0.0	0.0	0.0
35	0.4103013137033802	0.0	0.0	0.0	0.0
36	0.4200011774232166	0.0	0.0	0.0	0.0
37	0.42672096253033215	0.0	0.0	0.0	0.0
38	0.4317023684466939	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	3645	0.0	138.86647	44
TCATACG	100	0.0	105.01416	44
TAAGCCA	2145	0.0	100.85277	44
TAAACGT	165	0.0	95.46742	44
ACGACAC	320	0.0	91.8874	44
TCGAATC	50	9.0221874E-10	83.69358	43
AACGACA	440	0.0	76.08507	43
TCGCAAA	645	0.0	73.2203	43
CCTATCA	4035	0.0	71.55956	43
CCCATAC	635	0.0	71.11196	44
ACCGCAT	60	1.996714E-5	70.009445	44
TTAAGCC	1870	0.0	67.453575	43
TATAGCG	45	3.8800517E-6	66.42348	43
CGATACT	65	7.203198E-9	64.37968	43
TGATCGA	115	7.617928E-9	63.921665	44
ACTTCTA	1250	0.0	61.328274	44
AGGCCGA	14785	0.0	59.69002	42
GTGTTAG	1425	0.0	59.57137	43
CATGACG	145	0.0	57.71971	43
ATTAAGC	2195	0.0	56.56393	42
>>END_MODULE